Disseminated sclerosis(DS) is an autoimmune demyelinating disease of the central nervous system(CNS) that attacks generally the youth.The common symptoms of DS are hypopsia,paralysis,abnormal sense,urine deposited,dysfunction of bowels,etc.Sometimes MS also injures neural advanced functions.Traditional Chinese Medicine(TCM) mainly defines DS as "atrophy syndrome" according to its clinic symptoms.During acute relapsing period,most DS patients present deficiency of yin of both the kidney and liver,occurring with blood stasis and phlegm.Moreover,high dose methylprednisolone can activate pathogenic fire to damage the yin that aggravates deficiency of yin.Along with repeated relapse and evolvement of DS,the body's condition will change into syndrome of deficiency of both the yin and yang.So,the drugs of nourishing both the kidney and liver yin,activating blood circulation and clearing phlegm should be applied first to treat DS. Besides,the drugs of clearing heat,activating yang,motivating flow of qi,dredging the Fu-organ,invigorating blood and regulating liver-qi should also be used according to the different syndromes.

On basis of syndrome differentiation of TCM, 1 case of brain stem glioma was treated with decoction and Xihuangwan produced by Tongrentang. She has lived past 3 years with good life quality. More attention should be paid to the function of liver, spleen and stomach. The method of checking liver-yang and smoothing liver-qi, strengthening spleen-qi and harmonizing stomach-qi were used. Smoothing liver-qi can keep good blood and liquid circulation, and can reduce phlegm and blood stasis in the body; Checking liver-yang can prevent brain being disturbed by internal wind. Strengthening spleen-qi and harmonizing stomach-qi was the source of qi-blood. Xihuangwan can directly arrive at brain tumor because of its specific fragrant, it can smooth the qi and remove the blood stasis, can clear heat and detoxify. Only with the combination of disease and syndrome differentiation, whole body and local tumor, dispelling xie and strengthening zheng, can this case be treated successfully.

Objective To investigate the effects of prednisone(PRD) on experimental allergic encephalomyelitis(EAE) and the mechanism.Methods Lewis rats were injected with mixture of guinea pig spinal extracts and Freund's adjuvant(FA) to induce EAE.PRD(5 mg/kg) was orally given ten days after the injection.The clinical manifestation of rats,their peripheral blood lymphocytes CD3~(+),CD4~(+),CD8~(+) and NK subsets,and pathology changes of brain and spinal were studied on day 26.Results In model CD3~(+) ratio is 63.2%,CD4~(+)ratio 46.8%,CD8~(+)ratio 17.4% and NK ratio 10.2%.In PRD CD3~(+) ratio is 69.5%,CD4~(+)ratio 51.8%,CD8~(+)ratio 18.1% and NK ratio 5.3%.PRD obviously decreased the clinical scores of EAE rats and peripheral blood NK ratio,increased CD3~(+) and CD4~(+)ratio,inhibited brain inflammatory reaction and demyelination.However,PRD didn't affect peripheral blood lymphocytes CD8~(+) and CD4~(+)/CD8~(+)ratio.Conclusions PRD is effective in the treatment of EAE rats,and the therapeutic mechanism may be contributed to the decrease of the peripheral blood NK ratio and increase CD3~(+) and CD4~(+)ratio.

Objective: To observe the clinical effect of Huoxue Tongqiao Decoction on nervous tinnitus. Methods: 60 Patients(90 ears) were randomly divided into treatment group(30 cases, 44 ears) and control group(30 cases, 46 ears). Patients of control group took orally sibelium and oryzanol. Based on the control group, patients of treatment group took Huoxue Tongqiao Decoction to treat tinnitus. Results: After treatment, the total effi cient of treatment group was 86.4%, while that of control group was 63.0%. There was signifi cant difference(?2=13.83,P

0.05).Conclusion:The combination of TCM with western medicine was superior to simple western medicine in treating multiple sclerosis.Regulation of cytokines(TNF-? and IL-12 p40) in plasm may be partial reasons for the clinical effects of treatment based on syndrome differentiation.

Insomnia is a commen disease which disturbs life of modern persons terribly.As usual,insomnia means the subjective experience the short time of sleep and poor sleep quality which will influence the normal activity in the day-time.Here are the manifestations of insomnia:tired,dizziness,headache,tinnitus,palpitation,not concentration,poor efficiency etc.In this article,through the study on traditional twelve two-hour thery,the author connects the theory to the real reasons of insomnia and finds out the suitable time for patients to take the medicine to get twice the power with half the effort.

Kidney deficiency is the core of the currence of multiple sclerosis.Invigorating the kidney is the core in treatment of multiple sclerosis,though there are some other commonly used methods,such as dissipating phlegm,promoting blood flow,clearing heat,dispelling dampness and diuresis in aspects of eliminating pathogen,and invigorating spleen for benefiting qi,dispersing stagnated liver-qi,calming mind for tranquillizing in aspects of regulating the function of organs.Either invigorating the kidney is used through the whole course of disease or used at the later stage,it plays an important role in treatment of multiple sclerosis.

0.05) in sex,attack frequency,cause of disease,MRI and EEG among 202 patients. The TCM syndrome of weak in spleen and phlegm excess was the main type of child patients,while deficiency in liver and kidney was common in middle-aged and old patients,and they had prominent statistical significance (P 0.05) in sex,attack frequency,cause of disease,MRI and EEG. The weak in spleen and phlegm excess type is common in children,and the deficiency in liver and kidney type is usual in middle-aged and the elderly. Long disease course manifests deficiency or deficiency mixed with excess syndromes,while short course mainly shows excess syndromes. Earlier age of onset manifests deficiency or deficiency mixed with excess syndromes,and onset in youth and middle age often show excess syndromes. Discharge location in temporal lobe is often of wind phlegm type,while discharge location in frontal lobe often belongs to phlegm fire disturbing upper body type. Weak in spleen and phlegm excess type often can be seen in general discharge.

Objective To observe the immunohistochemical expression of lymphocyte subpopulationin in brain and spinal cord tissue,and explore the mechanism of Zuogui wan and Yougui wan on rats with experimental autoimmune encephalomyelitis(EAE).Methods Lewis rats were immunized with the myelin basic protein(MBP).120 rats were grouping randomly into normal group,EAE group,prednisone group,Zuoguiwan group and Youguiwan group after post immunization(PI).Rats in normal group and EAE group were administered normal soline,each 3 mL/d.Rats in prednisone group were administered suspension of prednisone after developed clinical signs,each 5 mg/kg.Rats in Zuoguiwan group were administered suspension of Zuoguiwan,each 2 g/kg,and rats in Youguiwan group were administered suspension of Youguiwan,each 3 g/kg.On 15th and 27th day after PI,rats were killed and the immunohistochemical staining was performed on the sections of brain and spinal cord.Results On 15th and 27th day after PI,the expression of CD4+ in brain and spinal cord tissue of EAE group were significantly higher than that in normal group(P

Objective To observe the effects of adiponectin on mRNA and protein expressions of connective tissue growth factor (CTGF) in hepatic stellate cells (HSCs) and the levels of procollagen type Ⅲ (PC Ⅲ) and hyaluronic acid (HA).Methods Cultured rat HSCs were treated with different concentrations of adiponectin.CTGF mRNA and protein expressions were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and Western Blot.The levels of PCⅢ and HA were detected by enzymelinked immunosorbent assay (ELISA).Results Compared with the control group,the result of RT-PCR showed that the four groups had different degrees of inhibitory effect,of which group D exhibited the strongest inhibitory effect.The absorbance ratio was 1.54 ±0.18,1.21 ±0.14,0.96 ±0.10,and 0.79 ± 0.08,respectively (t =2.42,P ＜0.05;t =2.73,P ＜0.05;t =3.28,P ＜0.01;t =4.67,P ＜0.01).Western Blot also indicated that four groups had different degrees of inhibitory effect,of which D group exhibited the strongest inhibitory effect.The ratio of integral absorption was 1.54 ± 0.18,1.21 ±0.14,0.96±0.10,and 0.79 ±0.08,respectively (t =2.84,P ＜0.01;t =4.05,P ＜0.01;t =6.25,P ＜0.01;t =9.72,P ＜0.01).The levels of PCⅢ and HA secreted in culture media were also decreased.It was significantly decreased with the concentration of adiponectin increased.Conclusion Adiponectin can inhibit the levels of PC Ⅲ and HA,which may be achieved through reducing CTGF mRNA and protein expressions.