Liver cancer is one of the most common malignant tumors in the world.The mortality of patients with liver cancer remains high e-ven with increasing progress in therapies.Early diagnosis is helpful for prolonging the survival of patients,so new biomarkers need to be ex-plored for timely diagnosis and treatment of liver cancer.The present research focuses on tumor-associated antigens (TAAs)and their cor-responding autoantibodies as serological biomarkers of liver cancer.The production of autoantibodies and the TAAs and anti-TAAs currently found in the liver cancer tissue are summarized.In addition,it is pointed out that these newly found autoantibodies have relatively high sen-sitivity and specificity for the early diagnosis of liver cancer.

Bevacizumab is an efficient and low-toxicity monoclonal antibody towards serum ventricular endothelial growth factor.At present,bevacizumab has been approved by FDA to use clinically for kinds of cancer,such as colorectal cancer,lung cancer,kidney cancer and breast cancer.As more and more patients received bevacizumab cliniclly,it's toxicities are also focused on increasingly.

The occurrence and outcome of cancer is related to the involved gene;Detecting the expres-sion of gene in body by the real-time PCR technology can diagnose and evaluate the prognosis of patients early. Through analyzing the abnormal genes and molecular,designing and studying the targeted drugs can achieve the therapeutic effect of steady,accurate,relentless,at the same time to reduce side effects and to improve the quality of life.

Objective To extract the flavomids from orange peel and determine the content of total flavmoids in orange peel. Methods The total flavonoids in orange peel were determined by using hesperidine as standard sample and edible alcohol as extraction solvent. Results The total content of flavonids is 19.44 g/kg. Conclusion The method is simple and reproducible. It provides a scientific basis for using orange peel.

Objective To determine the colorific value of Matrimory Vine in Qinhai. Method Calorific value of Qinghai matrimony-vine was determined by the method of oxygen bomb type-calorimeter instrument. Results Combustion heat of Qinghai matrimony-vine was 18.36 kJ/g. Conclusion The method is easy and reliable, and its principle is accurate. The data may provide a scientific basis for people’s diet structure and medical use.

Objective:To observe the dynamic expression of mRNA of TLR4 and TLR9 in Lewis rats with experimental autoimmune neuritis(EAN) and the effect of TWP on the disease.Methods:Male Lewis rats were immunized with P0 180-199(100 microgram),TWP was profused into post-immunization rats’ stomach daily.The clinical signs of rats and pathological changes in the sciatic nerves were observed.TLR4 and TLR9 were detected by RT-PCR dynamically which spleens,sciatic nerves and peripheral blood lymphonodes as sample.Results:EAN group got the peak of clinical score at the 17 d.p.i,and ameliorated obviously at 33 d.p.i,and the mRNA expression of TLR4 got the peak at the 16 d.p.i,then reduced gradually(P

Objective:To investigate the in vitro effect of capsaicin on four major rat cytochrome P450 ( CYP) enzymes using a cocktail probe drug method. Methods:The in vitro incubation was divided into capsaicin group and the control group. Rat liver micro-somes, probe drugs, capsaicin at various concentration ( buffer solution in the control group) and cofactors were cultured altogether for 20 min. After the culture, the concentration of metabolites was determined by HPLC to assess the activities of enzymes. IC50 value of capsaicin on every isoform was calculated using Graphpad prism 5. 0. Capsaicin and hepatic microsomess were pre-incubated respec-tively for 0, 5, 10, 15, 20 and 30 min, and then the relative activity of the four isoforms at different time was calculated. Results:The activity of the rat liver microsomes enzyme CYP450 isoforms (CYP1A2, CYP2C11, CYP2E1 and CYP3A2) was all inhibited by capsaicin in vitro with IC50 value of 36. 21, 17. 19, 51. 64 and 18. 86 μmol·L-1 , respectively. Pre-incubation could not increase cap-saicin inhibitory activity against the four CYP enzymes. Conclusion:Capsaicin shows inhibition on CYP1A2, CYP2C11, CYP2E1 and CYP3A2 in rat liver microsomes in vitro without pre-incubation time-dependent property.

Objective E cadherin catenin complex mediates cell cell adhesion and plays an important role in maintaining normal architecture and function of epithelial tissues. We evaluated the expression patterns of E cadherin, ? catenin, and ? catenin in gastric carcinoma and determine the potential relationship between E cadherin catenin complex and tumor clinicopathological features as well as patient survival. Methods Immunohistochemical staining of E cadherin, ? catenin, and ? catenin in 148 gastric carcinomas was performed. Results The abnormal expression rates of E cadherin, ? catenin and ? catenin in the tissues of gastric carcinomas were 44.6%, 73.6% and 43.2%, respectively. The total abnormal expression rate of E cadherin complex in 148 cases of gastric carcinoma was 91.9%. Reduced expression of E cadherin, ? catenin and ? catenin were significantly correlated with tumor differentiation status ( P

The intestinal microecosystem is composed of natural microflora, intestinal epithelial cells, and intestinal mucosal immune system. Nonalcoholic fatty liver disease (NAFLD) is a metabolic stress-induced liver injury associated with insulin resistance and genetic susceptibility. In recent years, there has been increasing evidence showing the involvement of imbalanced intestinal microflora in the pathogenesis of NAFLD. Overgrowth of intestinal microflora, increased permeability of intestinal mucosa, intestinal endotoxemia, and production of inflammatory cytokines play important roles in the development of NAFLD. Further studies on the relationship between intestinal microflora imbalance and the pathogenesis of NAFLD may shed light on the treatment and prevention of NAFLD.

Objective:This study was conducted to investigate Epstein-Barr virus (EBV) infection in gastric cancer in Wuwei ar-ea of Gansu province (China) and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas (EBVaGC). Meth-ods:Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-en-coded small RNAs (EBERs), miR-101, EZH2, and COX-2, in gastric cancer tissues (n=120) and the corresponding adjacent tissues (n=120). Results:The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant (P<0.05). The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric can-cer (EBVnGC) tissues were significant (P<0.05). The infection of EBV in gastric cancer tissues was positively related to miR-101 ex-pression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues (P<0.05). Conclusion:EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.