Objective To study the quality of life(QOL) and its associated factors in patients with traumatic brain injury (TBI) . Methods The 148 TBI patients treated and discharged from department of neurosurgery Chongqing beibei district people′s hospital were selected out and involved in the investigation .the World Health Organization health-related QOL BREF (WHOQOL-BREF) was used to investigate the QOL .116 valid questionnaires were obtained .Results The scores of physiological ,psychological ,social relationship ,environment domains of QOL among the patients with TBI were (69 .37 ± 17 .46) ,(59 .16 ± 14 .47) ,(54 .17 ± 14 .64) , (49 .33 ± 18 .33)scores .Multiple stepwise regression analysis results showed that ,age ,place of residence ,brain injury parting were risk factors for brain injury patient's quality of life ,GOS scores ,degree ,discharge time interval were protect factors (P< 0 .05) . Conclusion The QOL of the patient with TBI discharged from the hospital was lower than the domestic general population .The patient′s age ,residence ,educational level ,the type of TBI ,GOS score ,the time to discharges were the main influencing factors .

OBJECTIVETo screen long non-coding RNA (lncRNA) associated with radiosensitivity in colorectal carcinoma cell lines.

METHODSColony formation assay was performed in colorectal cancer cell lines HT29, SW480, RKO, Lovo and HCT116 after irradiation with different radiation doses. Radiation sensitivity of these 5 cell lines was detected through survival fraction at 2 Gy (SF2 value). High-throughput lncRNA chip was used to screen lncRNA genes with expression differences more than 2 folds among SW480, RKO and Lovo. Further experiment on the expression differences of lncRNAs selected was conducted by realtime PCR.

RESULTSThe radiosensitivity order of these 5 cell lines from low to high (SF2 value from high to low) was HT29 (0.83 ± 0.03), SW480 (0.69 ± 0.02), RKO(0.53 ± 0.02), Lovo (0.47 ± 0.05), HCT116 (0.32 ± 0.03) (P < 0.01). Five lncRNAs associated with radiation sensitivity were screened. Among them, expression levels of R05532, NR_015441, and NR_033374 were positively correlated with radiation resistance(all P < 0.01), and expression levels of the other 2 lncRNAs, NR_073156 and AA745020, were not correlated with radiation resistance of colorectal cancer cells (both P>0.05).

CONCLUSIONSlncRNA R05532, NR_015441 and NR_033374 may be used as the predictive marker of radiosensitivity of colorectal cancer cells. Higher expression of these genes shows radiation resistance.

Cell Line, Tumor ; Colorectal Neoplasms ; genetics ; radiotherapy ; Humans ; Oligonucleotide Array Sequence Analysis ; RNA, Long Noncoding ; genetics ; Radiation Tolerance