Objective To observe clinical effect of Chinese medicine combined chemotherapy in the treatment of elderly patients with advanced non-small cell lung cancer. Methods 61 cases of elderly patients with advanced non-small cell lung cancer patients were randomly recruited into two groups. 30 cases in treatment group, which was treated with Chinese medicine combined single-drug chemotherapy, 31 cases in control group, which was treated with Chinese medicine combined combination-chemotherapy. After 42 days of 2 cycle, the clinical effect of both groups was observed. Results The treatment group was superior to the control group on improvement of quality of life, symptomatic improvement, the toxicity of chemotherapy and treatment costs. The difference between the two groups were significant (P< 0.05) . Conclusion Chinese medicine combined single-drug chemotherapy in the treatment of elderly patients with advanced non-small cell lung cancer is superior to combined combination-chemotherapy.

This paper described the progress of treating elderly patients with advanced non-small cell lung cancer both domestically and abroad. The advantages of Chinese medicine and Chinese medicine combined with chemotherapy were emphasized.

Objective To analyze the development, treatment, death cause and survival situation of malignant tumor in aging patients Method Retrospectively analysis was made on 106 aging patients died of malignant tumors, who received the treatment in our hospital. Result Of all 106 patients, 102 patients were in advanced stage(Ⅲ+Ⅳstage), occupying 96.23%,most of who also had some other basic senile diseases; 96.23% patients had been treated with TCM, which exceeded the ones who were treated with chemotherapy, radiotherapy and operation. Some aging patients with tumor had better survival time than other age patients; the top three causes of death for tumor were respiratory failure, infection and congestive heart failure. Conclusion Most of aging patients with malignant tumor were in advanced stage, TCM treatment is easily to be accepted by patients, due to its fewer side effects. In the meantime, we should treat the basic senile diseases positively and prevent their complications.

Objective To develop a set of Immediate communication system of remote medical monitoring based on Internet,which is mainly used on the patient's condition for remote on-line monitoring.Methods The developing platform was Visual C++ 6.0 and the programming language was C++.The development of class libraries MFC(Microsoft Foundation Class) applied by Microsoft was also used.The real-time ECG images could be transmitted unhindered in the system.Results Medical care person could see the real-time ECG in system monitoring terminal from the client so that diagnostic results could be fed back timely to the client.Conclusion The development of community health care system is a positive solution to achieve the goal of universal health care for everybody.Its ultimate goal is to change high-tech medical care into conventional technology.On this basis,the function of hospital can be extended in to family care,thus really enhancing the quality of life

Objective To analyze the clinical features and interval of multiple primary carcinoma (MPC). Methods 52 patients with MPCs between October 2002 and May 2007 were reviewed. Results 7 patients had synchronous carcinoma (13.46%), and 45 had metachronous carcinoma (86.54%). The interval between the first primary cancer and MPC was from 0 to 31 years, averaged 7.1 years. Male was 7.3 years, and female was 9.3 years (P

Objective:To develop a new preparation process of PLGA microparticles for protein drugs by SPG membrane emulsifi-cation combined with W/O/W double emulsion-solvent technique. Methods:Lysozyme was used as the model drug to prepare the mi-croparticles. The influence of formula factors on the properties of the microparticles was studied, and the physicochemical properties, in vivo compatibility and degradation of the microparticles were investigated as well. Results:The drug loading of lysozyme-loaded mi-croparticles was 35%, the entrapment efficiency was 72. 43% and the average size was 63. 89 μm with PDI of 0. 675. DSC and FTIR showed that lysozyme was entrapped in the microparticles. The microspheres had promising biocompatibility and sustained degradation in vivo. Conclusion:The paper describes a new satisfactory preparation process of PLGA microparticles for protein drugs with good in vitro and in vivo properties.

Objective:To establish a patient-derived xenotransplantation (PDX) animal model of gastric cancer, and observe the anti-cancer effect of chemotherapeutic drugs on this model.Methods:Human gastric cancer tissues were inoculated into the subcutaneous tissues of both axillaries of NOG mice and were subcultured for 3 generations. The tumor tissues of the third-generation NOG mice were selected and inoculated into the subcutaneous tissues of left axillary of 21 severe combined immunodeficiency-non-obese diabetes mellitus (SCID-NOD) mice to establish PDX mouse model of gastric cancer. The inoculated mice were divided into control group (mice received only 0.9% sodium chloride injection), oxaliplatin group, cisplatin group, paclitaxel group, fluorouracil group, tegafur, gimeracil and oteracil porassium capsules group and capecitabine group, with 3 mice in each group, and the corresponding drugs were given. The mice survival status, tumor volume and tumor weight at different times were recorded. Mice were sacrificed on the 61st day of administration, and the tumor inhibition effects of 6 kinds of chemotherapy drugs on the PDX model of gastric cancer were evaluated.Results:After being subcultured for 3 generations, the stability of tumor transmission in PDX animal model of gastric cancer was improved, and the homogeneity of tumor growth was good at the initial stage. At the early stage of administration, the model was more sensitive to oxaliplatin, fluorouracil and capecitabine, and the tumor growth inhibition (TGI) values on the 31st day were 63.37%, 52.11% and 78.48%, at the end of administration, the model had the best sensitivity to capecitabine with a TGI value of 59.22% and a tumor inhibition rate of 58.65% on the 61st day. The TGI curve after administration showed that paclitaxel had no obvious anti-tumor effect, cisplatin had the worst anti-tumor effect, and the model had poor tolerance to tegafur, gimeracil and oteracil porassium capsules.Conclusion:The PDX animal model of gastric cancer is successfully established, and capecitabine has the best tumor suppressive effect on this model.

In order to investigate if there exists interaction between mitogen-activated protein kinase (MAPK) and signal transducer and activator of transcription 3 (STAT3) protein, and how the interaction regulates tumor necrosis factor-? (TNF-?) transcription activity, the human p38 and extracellular-signal regulated protein kinase 2 (ERK2) genes were amplified from human flag-p38 and flag-ERK2 by polymerase chain reaction (PCR) and cloned into pcDNA3-HA. Protein expression of the plasmids was examined by Western blotting. Co-immunoprecipitation was used to identify if there exists interaction between MAPK and STAT3 proteins. If the interaction was approved to be true, report gene system was applied to find how the interaction affect transcriptional expression of TNF-?. After STAT3 pathway was inhibited by RNA interfering, the action on TNF-? activity was determined. The results of DNA sequencing and enzyme digestion showed that the cloned p38 and ERK2 genes were correct, to be 1 080 bp or so. p38 and ERK2 proteins were expressed in 293T cell to be approximately 40 ku. Co-immunoprecipitation data showed that p38 and ERK2 proteins integrated with STAT3 protein in vivo. TNF-? reporter gene activity results found that protein complex of p38-STAT3 and ERK2-STAT3 coordinately increased TNF-? activity. After STAT3 was interfered, the TNF-? activity markedly decreased. These data indicated that there exists interaction between p38 and STAT3 protein, ERK2 and STAT3 protein. The complex of the proteins can coordinately regulate TNF-? expression. After interfereing STAT3 pathway, the coordinated action on TNF-? transcription activity might be obviously reduced.

Objective To explore ICSI treatment process in the different proportion of oocytes maturation and dosage of gonadotropin affecting embryo quality and clinical outcome. Methods From Jan. to Dec. 2015,we retrospectively analyzed the patients from the center line of ICSI cycle ,divided them into three groups according to the maturity of oocytes. The 3 groups were due to maturity rate of oocytes in 50% in group A(n = 19),50% 0.05). Group A had the best egg rate,high quality embryo rate,clinical pregnancy rate than the other two groups (P < 0.05). In pregnancy and non-pregnancy groups ,the dosage of gonadotropin in the non-pregnancy group was obviously higher than that in the pregnancy group in oocyte maturation in the same proportion interval(P<0.05). Conclusion In ICSI treatment process,the high dose of gonadotropin and rising rates of oocytes maturation are not conducive to the development potential of the egg and clinical outcome,so the dose of gonadotropin and oocyte maturation ratio should be controlled in a certain range to collect and protect the best dominant egg which matches to the state of the patient.

In this cross-sectional study, consecutive Chinese Han ethnic inpatients in the Division of Cardiology, the First Affiliated Hospital of Soochow University from January 1, 2010 to December 31, 2013 were included. According to the exclusion criteria, 9 587 patients [1 604 with type 2 diabetes mellitus (T2DM) and 7 983 without T2DM]were obtained for final analysis. Logistic regression model was used to analyze the association of ApoB/A-1 ratio with T2DM and the possible interactions between ApoB/A-1 ratio and other risk factors. The results showed that the distribution of ApoB/A-1 ratio was positively skewed in Chinese Han ethic population. The median of ApoB/A-1 ratio of female was lower than that of male (0.68 vs 0.73,P<0.01). In all groups, the proportion of T2DM was increased with the raised ApoB/A-1 ratio. By the stratification analyses of sex, age, coronary artery disease, and the use of statins, ApoB/A-1 ratio was still correlated with T2DM. There existed significant interactions between ApoB/A-1 ratio and the smoking status or creatinine in T2DM.