Objective:To clone and analyze a full-length cDNA encoding mouse integrin ?7,and repair the mutation of ?7 cDNA that caused the change of amino acids. Methods:The cDNA of ?7 gene was amplified by RT-PCR using the total RNA as extracted from mouse small intestine Peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed E.coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of ?7 cDNA that caused the change of amino acids was repaired. Results:The cDNA of mouse ?7 has an complete open reading frame with a length of 2418 bp,which encodes a product of 806 amino acid,and has 10 base pairs mutation of ?7 gene and 5 base pairs mutation that caused the change of amino acids was repaired. Conclusion:The cDNA of mouse ?7 was cloned successfully,which posed a basis for further researching on its biological function.

Objective To study the quality of life(QOL) and its associated factors in patients with traumatic brain injury (TBI) . Methods The 148 TBI patients treated and discharged from department of neurosurgery Chongqing beibei district people′s hospital were selected out and involved in the investigation .the World Health Organization health-related QOL BREF (WHOQOL-BREF) was used to investigate the QOL .116 valid questionnaires were obtained .Results The scores of physiological ,psychological ,social relationship ,environment domains of QOL among the patients with TBI were (69 .37 ± 17 .46) ,(59 .16 ± 14 .47) ,(54 .17 ± 14 .64) , (49 .33 ± 18 .33)scores .Multiple stepwise regression analysis results showed that ,age ,place of residence ,brain injury parting were risk factors for brain injury patient's quality of life ,GOS scores ,degree ,discharge time interval were protect factors (P< 0 .05) . Conclusion The QOL of the patient with TBI discharged from the hospital was lower than the domestic general population .The patient′s age ,residence ,educational level ,the type of TBI ,GOS score ,the time to discharges were the main influencing factors .

Objective To observe the effect of lycopene on myocardial interstitial fibrosis and to explore the possible mechanisms involved.Methods Sprague-Dawley rat myocardial infarction (MI) model was established by left anterior descending coronary artery ligation.Established MI model rats received lycopene or saline.After 28 days,myocardial fibrosis was observed by Masson staining.The expressions of Ⅰ type collagen,matvix metalloproteina-ses-9 (MMP-9) and matrix metall opeptidaseg(MAPKs) protein were detected in the peri-infarcted zone by Western blotting.Results In MI group,collagen volume fraction (CVF) was increased and the protein expressions of collagen Ⅰ,P-p38 and MMP-9 were increased in the peri-infarcted zone as compared with the sham group [CVF:(21.68±4.63)％ vs.(8.21±2.17)％; collagen Ⅰ:(1.58±0.22) vs.(0.97±0.09); P-p38:(1.93±0.44) vs.(0.85±0.21); MMP-9:(4.85±0.47) vs.(1.03±0.59); all P＜0.05].Lycopene attenuated the increments of myocardial infarction-induced collagen Ⅰ,p38MAPK and MMP-9 expressions [collagen Ⅰ:(1.24 ± 0.24) vs.(1.58± 0.22) ; P-p38:(0.85 ± 0.21) vs.(1.93 ±0.44); MMP9:(1.77±0.28) vs.(4.85±0.47); all P＜0.05],and decreased the collagen volume fraction in the peri-infarcted zone [CVF:(15.17±2.56)％ vs.(21.68±4.63)％,P＜0.05] as compared with the MI group.Conclusions Lycopene may improve ventricular remodeling after MI by inhibiting p38MAPK signaling pathway and MMP-9 expression.

Objective To observe and evaluate the clinical curative efficacy of perineal stapled prolapse resection in the treatment of complete rectal prolapse.Methods 15 patients of complete rectal prolapse were all treated by perineal stapled prolapse resection,The anal function of the patients were evaluated according to the Wexner incontinence score standard.Results All patients were successfully completed the operation.Postoperative complications:postoperative bleeding in 2 cases,anastomotic stenosis in 2 cases.These symptoms were relieved after symptomatic treatment.The anal sphincter function was improved in all patients after operation.There were no obvious incontinence.The preoperative Wexner incontinence score was 13.5±1.8,andpostoperative Wexner incontinence score was 4.2±1.5,there were significant statistical difference between them(P<0.05).The mean follow-up were 18 months(2-30 months).There was no recurrence during the follow-up period.Conclusion Perineal stapled prolapse resection as a new type of operation had small trauma,simple operation,less complications and short-term curative effect of good characteristics.They can effectively improve the symptoms of anal incontinence in patients with complete rectal prolapse.

Objective To investigate the effect of XueBiJing injection(a concoction of Chinese herbs)on the expression of high mobility group box-1 protein(HMGB1)in the lung with acute lung injury of rats following delayed resuscitation after burn injury.Methods 78 male rats were randomly divided into sham scald group(n=18),scald group(n=30)and XueBiJing treatment group(n=30).In the scald group and XueBiJing treatment group,4ml/kg normal saline or XueBiJing was intravenously injected 2 hours after scald injury.In the latter two groups,rats were subjected to 30% TBSA full-thickness scald injury,and fluid resuscitation was delayed.The animals were sacrificed at 8h,24h and 72h after injury,respectively.Lung tissue samples were collected to determine the expression of HMGB1 mRNA and protein,and the activity of pulmonary myeloperoxidase(MPO).HMGB1 mRNA level was semi-quantitatively measured by RT-PCR using GAPDH as an internal standard,and protein expression of HMGB1 was determined by Western blot.Results Compared with sham scald controls,both mRNA and protein expressions of HMGB1 were significantly enhanced in the lung at 8-72h after scald injury(P

Objective To investigate whether CD4~+ CD25~+ regulatory T cells (Treg) from C57BL/6J mice express alpha7 nicotinic acetylcholine receptor (α7nAChR). Methods CD4~+ CD25~+ regulatory T cells were isolated from mouse splenocytes with a CD4~+ CD25~+ regulatory T Cell isolation kit (Mihenyi Bio-tee). The purity of isolated Tregs was analyzed by flow eytometry. Expressions of α7nAChR in mouse CD4~+ CD25~+ Tregs were examined by immunofluorescence staining, Western blotting, and reverse transeription-PCR, respectively. Results It was revealed by flow cytometry that Tregs could bind alpha-bungarotoxin (α-BGT)-F/TC, a specific α7 nAChR antagonist. Moreover, a positive binding to α-Bgt was also observed on the cell surface of Treg, as viewed by fluorescent confoeal microscopy. In addition, a clear band of a7nAChR with a molecular mass of approximately 55 kD was found from Tregs by Western blotting analysis, and α7nAChR mRNA was expressed with the expected size of 199 bp from Tregs by reverse transcription-PCR. Conclusion Natural CD4~+ CD25~+ Tregs from mice express α7nAChR.

AIM To investigate the potential effect of riboflavin on the growth of probiotic strains Bifidobacterium adolescentis ( B. adolescentis) and Bacillus cereus ( B. cereus) . METHODS By means of routine bacterial quantitative culture, Gram's staining, and light microscopy, changes in B. adolescentis and B. cereus counts were detected in the presence of riboflavin at different concentrations (1, 0 5, 0 25, 0 g?L -1 ). RESULTS ①The counts of B. adolescentis increased by 10 to 100 fold in 1g?L -1 riboflavin group after 48 h, and 10 to 390 fold in 0 5 g?L -1 and 0 25 g?L -1 riboflavin groups within 72 h as compared to that without riboflavin supplement. Meanwhile, the chain lengths of B. adolescentis were markedly longer in culture system with riboflavin than those without. ②Compared with 0 g?L -1 riboflavin group, the count of B. cereus increased significantly in 0 5 g?L -1 riboflavin group at 36 h, and in 0 25 g?L -1 riboflavin group within 72 h, while it decreased by 50 to 100 000 fold in 1 g?L -1 riboflavin group within 72 h. Likewise, the chain length of B. cereus was markedly longer in 0 5 g?L -1 and 0 25 g?L -1 riboflavin groups, together with the delayed spore formation of B. cereus. ③The 6 month survival rates of B. adolescentis and B. cereus counts in preparations (depositing at 4℃) with 0 5 g?L -1 riboflavin were much higher than those without riboflavin supplement. CONCLUSION Riboflavin in concentrations of 0 5 g?L -1 and 0 25 g?L -1 could markedly enhance the growth of B. adolescentis and B. cereus. 0 5 g?L -1 riboflavin might be beneficial to improve the survival rate of B. adolescentis as well as B. cereus when storing for a long term.

Objective:To study the effect of the cyclic-tension force on the expression of matrix metalloproteinase-3 ( MMP-3) mRNA in osteoclasts. Methods: Bone marrow cells were cultured and induced by the combination of IL-6 and 1,25(OH) 2D 3 and identified. The bone marrow cells were seeded onto elastic 6-well culture plate at a density of 2?105 cells/ml in 2ml in each well and cultured for 7 days. Then the cells were subjected to 12% elongation by a strain unit at 6 cycles/min (i.e.5-s elongation and 5-s relaxation) . After 24 hours of stretching, the expression of MMP-3 mRNA in osteoclasts were determined by in situ hybridization analysis. Results:The stretched osteoclasts showed enhanced MMP-3 mRNA expression level. Conclusion: The mechanical stretching may affect the bone-resorbing activity by up-regulated the expression of MMP-3 mRNA in osteoclasts.

Fourteen kinds of ethyl ester of fatty acids form Hai Haozi(Sargassum pallidum) are analysed by GC methods.They are identified by comparision of their rentention time with those of known standards and quantitated by using an internal standard.It shows that this drug contains lots of saturated and unsaturated fatty acids.The analytical resulits may provide material basis for futher research of Hai Haozi.

PurposeTo study the pharmacokinetic characteristics of omeprazole in Chinese extensive and poor metabolizers.Methods The pharmacokinetics of omeprazole was studied in eighteen healthy volunteers. After a single oral dose of omeprazole capsule(40 mg), the plasma concentrations of omeprazole and its two metabolites, 5-hydroxyomeprazole and omeprazole sulfone, were determined with reversed HPLC method. The plasma concentration-time data were analyzed to estimate the pharmacokinetic parameters. Results Both plasma omeprazole concentration and the pharmacokinetic parameters exhibited marked interindividual variation. The metabolic ratio (MR= plasma omeprazole/5-hydroxyomeprazole) obtained 3.5 h after medication was used to distinguish between extensive and pcr)r metabolizers (EMs, PMs). The variances of AUC(0-12) caused by the two metabolizer phenotypes accounted for 75.4 % of the total interindividual variances. AUC(0-12) of omeprazole was (1 971.78 ±1 221.78)ng·h/ml in EMs( n=12) and (8 587.18±2 855.48) ng·h/ml in PMs (n = 6),respectively (P<0.01),and CL in EMs and PMs was estimated as (16.00±9.71) and (4.79±1.32) L/h (P<0.01). Accordingly,significantly lower level of plasma 5-hyclroxyomeprazole was found in PMs, revealing a slower hydroxylation rate compared with EMs. Conclusions The results suggest that individualized dose regimen of omeprazole, based on identification of metabolizer phenotype, can be of great benefit from the viewpoint of pharmacoeconomics.