Objective To explore the effect of different speed of bladder irrigation after transurethral resection of prostate.Methods 477 cases of benign prostatic hyperplasia,who received transurethral resection surgery were collected.According to the date of surgery,they were divided into observation group(in a single day,249 cases)and control group(double day,228 cases).The normal saline was used in the two groups,and the control group was washed with the conventional bladder irrigation,and the observation group was treated with the early rapid(no speed limit opening)and the routine velocity alternating flushing.The incidence of bladder spasm,the incidence of bladder spasm,the bleeding of bladder and the changes of heart rate and the mean arterial pressure of 24h were compared. Results Urine turned clear time,the total fluid,postoperative 5d red blood cell count of the observation group[(19.8 ± 8.3)h,(44.7 ±6.1)L,(25.8 ±11.3)months/μL]were significantly lower than the control group[(25.4 ±9.0)h, (52.3 ±7.4)L,(33.4 ±10.1)months/μL],the differences between the two groups were statistically significant(t =-6.270,-2.417,-10.459,all P <0.05).The incidence rate of bladder spasm,seizure frequency and duration per capita of the observation group[24.5%,(2.2 ±0.6)times,(15.4 ±4.6)min]were significantly lower than the control group[46.9%,(6.7 ±1.2)times,(27.6 ±5.1)min],the differences between the two groups were statistically significant(χ2 =8.725,t =-13.250,t =-18.066,all P <0.05).The postoperative rinse 1h,2h and 24h heart rate,mean arterial blood pressure had no statistically significant differences(all P >0.05).Conclusion The early rapid and conventional alternating washing method can improve the seizure of bladder spasm after prostatectomy,and reduce the bleeding of bladder.

Objective To observe the effect of acupuncture analgesia on delivery and observe the result of analgesia in mother and infant.Methods Totally 324 primiparae were randomized into four groups:Electro-acupuncture group,82 cases,transcutaneous electrical nerve stimulation(TENS)group,82 cases,control group,81 cases,and spinal-epidural analgesia group,79 cases.The VAS scores of pain,degree of satisfaction of analgesia,outcome of delivery and changes of endorphin concentration were observed during the delivery and after.Results Compared with the TENS group and control group,viewing from the VAS scores of 30~120 minutes after analgesia,the effect in the electro-acupuncture group was better,but not as good as that in the spinal-epidural analgesia group,the difference was significant(P

Objective To test the content of menthol in Chuanbei Pipa Syrup(CPS)by GC, and to evaluate its quality. Meth-ods The content of menthol in CPS was determined by capillary gas chromatography. DB-WAX capillary column (30 m ×0. 319 mm × 0. 50μm) was used with nitrogen as carrier gas. The precolumn pressure was 500 kPa and the column temperature was at 110 ℃ with split ratio of 25 : 1. The temperature was set at 250℃ for sample injection and for the detector. Results The content of menthol in CPS produced by Henan Pharmaceutical Co. , Ltd. , Zhengzhou Pharmaceutical Co. , Ltd. and Yunan Pharmaceutical Co. , Ltd. was 0. 154 mg/mL, 0. 203 mg/mL and 0. 109 mg/mL, respectively. Of them, the men-thol content in CPS produced by Zhengzhou Pharmaceutical Co. , Ltd. met the requirements of Pharmacopoeia(2005 edition), and that of the other two species was slightly lower. Conclusion The methods are convenient, reliable and accurate for the de-termination of menthol content in Chuanbei Pipa Syrup.

Objective To analyses the CT characteristics of primary malignant ovarian tumors and metastatic ovarian carcinoma and its diagnosis and differential diagnosis.Methods CT findings in 31 patients with pathologically-proved malignant ovarian tumors,encountered during 1999-04~2003-11 were retrospectively analyzed.The observation of the lesion was focused on the following aspects:location,internal density,septa,wall,intra-cystic and extra-cystic vegetation,the composition of the soft tissue,ascites,peritoneum,mental implantation and metastatic lymphadenopathy.The characteristic of each in both primary malignant ovarian tumor and metastatic ovarian tumor were compared.Results Among the 24 primary malignant ovarian tumors,13 patients had lesions in both sides of ovary,type Ⅱa:n=3,Type Ⅱb:n=11,Type Ⅱc:n=7,Type Ⅲ:n=3.Among the metastatic ovarian carcinomas,Type Ⅱa:n=6,Type Ⅱc:n=1.Conclusion Type Ⅱb and Ⅱc are commonly seen in primary malignant ovarian tumors.Type Ⅱa is always seen in metastatic ovarian carcinoma.The metastatic tumors often happen in the right peritoneum,epiploon,and liver."arborization and silk screen likely"are the characteristic representation of the metastatic ovarian carcinoma.

Objective To evaluate the relationship between maternal antepartum fever and early-onset neonatal bacterial sepsis.Methods A retrospective multi-centred clinical study of 404 neonates including 103 new borns with maternal antepartum fever and 311 neonates without was conducted.Results The frequency of early-onset neonatal bacterial sepsis was significantly higher in neonate with maternal antepartum fever than those without(4.9% vs 0.3%,P=0.001).Both maternal antepartum temperature ≥39℃ and maternal postpartum fever lasting several days was higher suggested the possibility of neonatal sepsis.Conclusions The neonates born with high risk factor of early-onset neonate bacterial sepsis require comprehensive lab evaluation including blood culture and uterine culture,clinical observation and aggressive treatment to decrease the mortality.

Objective To determine the prevalence of renal artery stenosis (RAS) and the efficacy of percutaneous transluminal renal angioplasty (PTRA) and stenting in patients with documented coronary artery disease.Methods Selective renal artery angiography or abdominal digital subtraction angiography was performed immediately after coronary angiography to detect and quantify RAS in 200 consecutive patients with known or suspected coronary artery disease. Eight severe stenotic renal arteries in 7 patients were treated with PTRA and stenting. All the lesions are considered to be atherosclerotic. Results RAS (defined as ≥50% diameter stenosis) was detected in 21 of 200 patients (10.5%) and 19 of 99 patients with coronary artery disease (19.2%) who had at least one coronary artery of ≥50% diameter stenosis. Procedure success rate was 100% in the 7 patients received PTRA and stenting. Blood pressure control improved in 74.1% patients with hypertension. Angiographic follow up was done in 3 patients in 3 to 9 months and no restenosis was found in the vessels.Conclusions It is necessary to routinely perform RAS for the patients with coronary artery discase undergoing coronary angiography to identify RAS, especially in those associated with hypertension, so as to diagnose and treat RAS in early stage. PTRA and stenting carries high success rate and appears to be an effective and safe treatment for RAS.

Objective To investigate the change of anxiety in patients undergoing coronary angiography. Methods The Spielberger State-Trait Anxiety Inventory was carried out in hundred and forty five patients, 104 men, 41 women, mean age (63?9) years, before and after the coronary angiography procedure respectively. Results The state anxiety score after the procedure was significantly decreased from 35.8?10.2 to 31.7?9.9(P

Objective To summarize the experience of seven-step nursing method used in the care to patients with spontaneous esophageal rupture.Method Twenty patients with spontaneous esophageal rupture were treated with the seven-step nursing method. Results All patients were discharged after cure.The follow-ups found no complications of esophageal stenosis,reflux esophagitis and chronic empyema.Conclusion The seven-step nursing method used for caring the patients with spontaneous esophageal rupture can promote the recovery of patients and reduce their suffering.

Objective To explore the antibacterial activity of extract of Ginkgo biloba leaves on Porphyromonas gingivalis in vitro ,and to provid pharmacological reference for developing a new type of antibacterial drugs in the treatment of periodontal disease.Methods This experiment was divided into negative control group,imipenem control group and different concentrations and forms of extract of Ginkgo biloba leaves groups.Solvent extraction method was used to extract the extract of Ginkgo biloba leaves, punching method and test tube method were performed to detect the antibacterial activity of extract of Ginkgo biloba leaves in anaerobic environment invitro and compared with Staphylococcusaureus and E.coli.By observing the antibacterial ring diameter and determination of the minimum bacteriostasis concentration (MIC),the antibacterial activities of extract of Ginkgo biloba leaves in vitro were measured.Results In the experiment of bacteriostatic ring,Porphyromonas gingivalis was treated with extract of Ginkgo biloba leaves,Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule concentrate and 1∶4 diluent,the bacteriostatic ring diameters were decreased with the decreasing of the concentration.The maximum bacteriostatic diameter of Ginkgo biloba extract was 1 6.5 mm,and the maximum bacteriostasis diameters of Ginkgo biloba leaf tablet and soft capsule were 15.3 and 14.5 mm,respectively;the bacteriostatic diameter of the exact of Ginkgo biloba leaves was bigger than those of Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule (P < 0.05), but there was no statistical difference of bacteriostatic diameter between Ginkgo biloba leaf tablet and Ginkgo biloba soft capsule (P> 0.05);E.coli and Staphylococcusaureus groups get the same results.When the concentration of extract of Ginkgo biloba leaves was more than 1.95 mg·L-1 ,there was no growth of Porphyromonas gingivalis but E. coli and Staphylococcus aureusa still grew;only the concentrations of exact of Girkgo biloba leaves were more than 6.25 and 12.5 mg· L-1 ,E. coli and Staphylococcus aureus didn’t grow;the bacteriostatic effect of extract of Ginkgo biloba on Porphromonas gingivalis was better than E.coli and Staphylococcus aureus . Conclusion Extract of Ginkgo biloba leaves has antibacterial effect on Porphyromonas gingivalis.

AIM: To investigate the effect of granulocyte colony stimulating factor (G-CSF) on arabinosyl cytosine (Ara-C)-induced apoptosis in HL-60 leukemia cells. METHODS: The proliferation of HL-60 leukemia cell was observed by hemopoietic cell culture. Apoptosis was measured by the morphology of apoptosis cell , the quantitation of DNA fragmentation with the diphenylamine reaction. The change in drug sensitivity was measured by “MTT”. RESULTS: G-CSF could stimulate the proliferation of HL-60 leukemia cell and colonies of cell increased to 76.5?18.0, compared to the control group (46.5?13.5. P