[Objective] To analyze the effect of operating and traditional Chinese drugs in treating appendicular abscess.[Methods] A study was made on 235 cases of appendicular abscess in acute period;strengthen post-operation support therapy,use antibiotics,meanwhile apply Chinese medicines.[Results] No case had stump fistula or intestinal fistula,among which,227 cases went out hospital after 7 days after operation;only 8 cases had incision infection,who also went out of hospital after 11~15 days.All cases were cured.[Conclusion] Operating combined with TCM in acute stage after operation was feasible,with satisfying result.

Background and purpose:As a regulatory factor of cytoskeletal structure,a close correlation existed between Tiam 1(T lymphoma invasion and metastasis inducing factor 1) and the invasion and metastasis of gastroenteric tumor.We aimed to observe the effects of Tiam 1 antisense oligodeoxynucleotides(Tiam 1 ASODN) transfection on the invasive and migratory potentials of gastric cancer cells in vitro and vivo.Methods:The higher invasive and migratory subgroup(MH) were separated from human gastric cancer cell line MKN-45(M0) by laminin adhesion method in vitro.Tiam 1 ASODN was transfected into MH cells with liposome,and the expression of Tiam 1 mRNA and protein was determined by RT-PCR and ELISA separately.Changes in the invasive and migratory potentials of MH cells in vitro and vivo after transfected with ASODN were observed by Boyden chamber test and inoculation into nude mice respectively.Results:The expression of Tiam 1 mRNA and protein in MH cells after transfected with 0.43 ?mol/L ASODN(0.162?0.018,0.982?0.119) was significantly lower than that of either Liposome-transfected cells(0.789?0.054,1.237?0.108),sense oligodeoxynucleotides(SODN)-Liposome-transfected cells(0.754?0.039,1.234?0.103) or No-transfected cells(0.801?0.065,1.290?0.182)(P

0 05),at six months it changed significantly (compared with normal P

Objective To investigate the relationship between the expression of T lymphoma invasion and (metastasis) inducing factor 1 (Tiam1), ras-related C3 botulinum toxin substrate 1 (Rac1) with the invasion and metastasis of gastric cancer. Methods The expressions of Tiam1 and Rac1 proteins in 60 cases of (gastric) cancer and paracarcioma gastric mucosa tissues were detected using Strept Avidin-Biotin Complex(SABC) immunohistochemical method,and the correlation between the expression of Tiam1, Rac1 and (clinicopathological) parameters of gastric cancer were analysed. Results (1)There was negative staining of Tiam1 protein in paracarcioma gastric mucosa tissues, while positive staining was detected in gastric cancer (tissues)(78.33%)(P 0.05). (4) The expression of Rac1 in patients with positive staining of Tiam1 was significantlyhigher than that in patients with negative staining(P

Objective To measure the fluorescence spectral information of UVRF in the facial skin of patients with acne vulgaris and seborrheic dermatitis and to comprehend the optical properties of UVRF.Methods The lesions were squeezed to extract the sebaceous secretions containing UVRF in the facial skin.Ocean Optics USB2000 +F02243 Spectrometer and the 308nm excimer laser were used as an excitation light source to measure the signal of the LIF spectrum of UVRF in 6 acne vulgaris and 4 seborrheic dermatitis patients' facial part,compared with protoporphyrin Ⅸ.Results 1-4 peaks were detected in 10 patients' UVRF specimen respectively.The fluorescence spectra's characteristic peak of UVRF in 10 patients was at 680 nm.Protoporphyrin Ⅸ was at 688 nm and more gentle and generous than UVRF's characteristic peak.Conclusions LIF system can be used to detect the fluorescence spectroscopy of UVRF.UVRF has similar spectral characteristics to protoporphyrin Ⅸ,but it is not the same species.

Objective To evaluate the effect of an equivalent uiform dose optimization (EUD) based optimization algorithm in sparing the organ at risk (OAR) of tumor treated with intensity-modulated radiation therapy (IMRT).Methods 10 patients of nasopharyngeal carcinoma (NPC),10 patients of esophagus cancer,6 patients of rectum cancer were randomly selected from all patients received IMRT treatment.Then physical optimization constraints (dose/dose-volume constraints) were designed as EUD optimization,while the physical objectives for targets and other OAR were unchanged.Results There was statistically significant difference in protecting OAR in 8 NPC patients of PLANED group compared with those of PLANNO_EuD group (t values were 2.590,2.352,3.656,2.148,2.283,3.683,2.192,2.353,2.146,2.276,2.126,P ＜ 0.05).There was no statistically significant difference in protecting OAR in 10 cases of esophageal cancer between PLANEUD group and PLANNO_EUD group (t value were 0.408,0.427,0.920,P ＞ 0.05).There was statistically significant difference in protecting OAR in 6 cases of rectum cancer of PLANED group compared with those of PLANNO_EUD group (t values were 2.332,2.693,2.279,2.244,P ＜ 0.05).Conclusion EUD optimization can reduce the doses to OAR which may be quite helpful to reduce the occurrence rate of OAR complications.

Objective To study the effects of CO2 pneumope ritoneum on the abdominal viscera metastasis and peritoneal implantation of gastric cancer cell line MKN45 during laparoscopic gastric surgery.Methods The gastric cancer cells metastatic model was eslhblished by injecting 1 ml(2×107/ml)human gastric cancer cell line MKN45 susDension into the caecum wall of 60 Balb/C mice.Two weeks later,the Balb/C mice were divided into CO2 pneumoperitoneum group(n=30)and laparotomy group(n=30). All mice were sacrificed 7 weeks later to investigate the abdominal viscera metastasis and peritoneal implantation of gastric cancer cells.Results Twenty-seven rats in CO2 pneumoperitoneum group were induced with tumor,including 8 with troear port implantation and 19 with organ metastasis;26 rats in laparotomy group were induced with tumor,including 7 with tracar port implantation and 19 with abdominal viscera metastasis,and the differences between the two groups had no statistical significance(x2=0.007,0.240,0.202,0.106,0.042,P＞0.05).Conclusions CO2 pneumoperitoneum does not stimulate the metastasis of gastric cancer(cells to peritoneum and abdominal viscera in Balb/C mice.Laparoscopic surgery for gastric cancer is safe and feasible.

BACKGROUND: Existing research shows that in situ regeneration of skin deep within the second degree bum wound and donor site wound healed without physical scarring, can promote three-degree burn wounds liquefied necrotic tissue removement, the growth of transplanted skin, reduce scar; scar-shift using the in situ regeneration is expected to reach significantly reduce scar symptoms, and to reduce the effect of scar, which have not be reported.OBJECTIVE: To observe effects of skin regeneration in situ method to remove scar in the treatment of hypertrophic scar. METHODS: A total of 32 patients with many hyperplastic scars, including 25 males and 7 females, aged 16-52 years, disease course of 1-11 years. Two similar scar regions were selected from each patient for self control. In the experimental group, scar removal, scar skin replantation after the application of in situ regeneration of the skin treatment using burn cream coated yarn. In the control group, scar removal, scar skin replantation after the application of traditional Vaseline covered by treatment. Curative effects were observed and compared. Scar hyperplasia was assessed using Vancouver Scar Assessment Scale assessment. RESULTS AND CONCLUSION: Replanted scar skin explants were survived in both groups. In the experimental group, healing speed and quality of wound surface were better than the control group (P< 0.05). After 6 months, the Vancouver Scar Assessment Scale assessment in the experimental group was better than control group (P < 0.05, P < 0.01). Scar caused by pain, itching and other symptoms disappeared, skin formation and color back to pre-implantation were significantly improved compared with the surrounding skin almost. Results indicated that with regarding to the lack of autologous skin source, large area of scar in patients with hypertrophic scars or unwilling to add a new donor site wounds in patients, in situ replantation method is an ideal approach.

Objective To investigate the anti-gastric cancer effects of dendritic cells tumor vaccine loaded with heat shock protein gp96 polypeptide complex. Methods Con A sepharose affinity chromatography and mono Q ion exchange chromatography were applied to purify gp96 polypeptide complex from gastric cancer tissues and DC was loaded with gp96 polypeptide complex to prepare tumor DC vaccine. Fluorescence-activated cell sorter (FACS) was used to detect surface molecules expression of dendritic cells. Enzyme-linked immunosorbent assay ( ELISA) was used to detect IL-10 and interferon-? (IFN-?) of the supernatant secreted by activated T lymphocytes and 51Cr release test was performed to evaluate the gp96 peptide specific CTL response. Results DCs derived from patient's peripheral blood mononuclear cells loaded with gp96 polypeptide complex had high express surface molecule of GD1? (79. 3 ?4. 1)% , CD80 (84. 3 ? 2. 4) % , CD83 (85. 7 ? 3. 2) % and HLA-DR( 83.4 ? 2. 9) %. The effector T lymphocytes induced by DCs vaccine loaded with gp96 polypeptide complex had rised killing effect to primary culture gastric tumourcells(58.47?10. 71)% when compared with SGC 7901 cells(23. 98 ?4. 16)%. The level of IFN-? (2875 ? 177.66 pg/ml) significantly increased and that of IL-10 (36 ? 6.72 pg/ml) decreased. Conclusion Autologous heat shock protein gp96 polypeptide complex/DC vaccine induces strong peptide complex specific CTL response with high specificity.

Objective To compare detection of STAT3 and CEA by nest RT-PCR and peritoneal lavage cytology examination(PLC) in predicting peritoneal micrometastasis in gastric carcinoma patients. Methods Peritoneal washings of 66 patients with gastric carcinoma and 12 patients with benign gastric diseases were collected to detect the mRNA level of STAT3 and CEA by nest RT-PCR.PLC were carried out by H-E staining.Results The positive rates of STAT3 mRNA and CEA mRNA in peritoneal washings were 43.9% and 48.5%,respectively.The positive rates of STAT3 mRNA and CEA mRNA were significantly higher than those of PLC(31.8%).The positive rates of STAT3 mRNA and CEA mRNA were concurrently increased with the depth of tumor invasion,TNM staging and pathogenesis.Conclusion Detecting STAT3 mRNA in peritoneal washings by nest RT-PCR can improve the sensitivity of free tumor cell detection in peritoneal washings,and may be used to predict peritoneal micrometastasis of gastric carcinoma.