BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.

BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix.
OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels.
METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28.
RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.

Objective To analyze the proportion of HPV infection among the patients in the gynecology and obstetrics department and urology surgery department of our hospital,distribution of HPV high risk and low risk subtypes and distribution characteristics of high risk HPV predominant subtypes in various age groups,and to understand the HPV infection situation in this area to provide reference for the prevention of cervical cancer and vaccine development aiming at this area.Methods The cervical exfoliated cells in 889 female patients in our hospital during 2014-2015 were detected by PCR reverse spot hybrid technology,26 HPV subtypes were detected,including 17 high-risk subtypes and 9 low-risk subtypes.Results Among 889 cells samples,168 cases of HPV positive were detected with the positive rate of 18.9%,which was dominated by single infection,148 cases of high-risk subtypes were detected,accounting for 84.5% of the total number of positive cases.24 kinds of HPV subtypes were detected.High risk subtype HPV45 and low risk subtype HPV57 were not detected.The top HPV high-risk subtypes in the detection rates were HPV52,HPV16 and HPV58,in young and middle-age women,HPV16 and HPV52 were predominant infection subtype,accounting for 84.5% of total positive cases.Women >60 years old were mainly infected by HPV58.Conclusion Different subtypes of vaccine can be developed and adopted according to these study results and by aiming at different ages for preventing and treating cervical cancer and increasing the female health level.

Objective: To investigate the quality of life of n ur ses in general hospital Method: 574 nurses in a general hospit a l were sampled and tested with WHOQOL-100 Results: Compared wi t h Chinese norm, the following fields and aspects had no significant difference They were fields of physiology, social relationship and aspects of medical servi ce and social security, of recreation There were significant difference to nor m in the other four fields and 22 aspects Among them, aspects of pain, sleep, d e pendence on medication or other medical service, sex life, and environment had l ower scores than the counterparts in norm Other aspects had higher scores than norm (p

Objective: To investigate factors related to quality of life among nurses. Methods: 1103 nurses were sampled from 5 hospitals and administered with WHOQOL-100. Results: There were significant differences between nurses' on major variables of quality of life. Levels of depression and anxiety had important influence on the quality of life of nurses. Conclusion: The quality of life of nurses was within normal range. Some variables such as mental health status and family factors might play an important role in nurses' quality of life.

Objective To build emergency specialist nurse training evaluation index system model.Methods By Delphi method,21 emergency care specialists participated in the consultation,using the Analytic Hierarchy Process (AHP)to build a hierarchical model,establishing judgment matrix,through statistical calculations to determine the indicators and weight coefficients of the index system of emergency specialist nurse training.Results Four first-level indicators,11 second-level indicators,31 third-level indicators emergency specialist nurse training evaluation system model were established.Conclusions Establishment of emergency nurse specialist training evaluation index system provides an effective method and reference to improve the overall capacity of the emergency specialist nursing team to ensure the quality of training of emergency nurse specialists.

A nitrite electrochemical sensor based on electrodeposition of zirconium dioxide nanoparticles on reduced graphene oxide modified electrode was successfully constructed for the detection of nitrite. The electrochemical behavior of the modified electrode was investigated by cyclic voltammetry and amperometric i-t curve. Under the optimal conditions, the amperometric i-t curve response of the electrode showed a linear relationship with nitrite concentration in the range of 3.0×10Symbolm_7-1.0×10Symbolm_6 mol/L and 1.0×10Symbolm_6-6.0×10Symbolm_6 mol/L, and the detection limit was 1.0×10Symbolm_7 mol/L (S/N=3). The fabricated sensor exhibited high sensitivity, good stability and high reproducibility. This sensor was applied for the detection of nitrite in sausage samples with favorable recoveries of 93.7％-110.4％ and relative standard deviation (RSD) of 1.6％-2.1％.

The beta-secretase is one of prospective targets against Alzheimer's disease (AD). A three-dimensional quan titative structure-activity relationship (3D-QSAR) model of Hydroethylamines (HEAs) as beta-secretase inhibitors was established using Topomer CoMFA. The multiple correlation coefficient of fitting, cross validation and external validation were r2 = 0.928, q(loo)2 = 0.605 and r(pred)2 = 0.626, respectively. The 3D-QSAR model was used to search R groups from ZINC database as the source of structural fragments. As a result, a series of R groups with relatively high activity contribution was obtained to design a total of 15 new compounds, with higher activity than that of the template molecule. The molecular docking was employed to study the interaction mode between the new compounds as ligands and beta-secretase as receptors, displaying that hydrogen bond and hydrophobicity played important roles in the binding affinity between the new compounds and beta-secretase. The results showed that Topomer CoMFA and To pomer Search could be effectively used to screen and design new molecules of HEAs as beta-secretase inhibitors, and the designed compounds could provide new candidates for drug design targeting AD.
Amyloid Precursor Protein Secretases ; antagonists & inhibitors ; Drug Design ; Hydrophobic and Hydrophilic Interactions ; Ligands ; Molecular Docking Simulation ; Quantitative Structure-Activity Relationship

Objective To evaluate the bactericidal effect of diode laser on Porphyromonas gingivalis (Pg),and to explore an optimized protocol for a safe dose of photodynamic therapy (PDT) to eliminate periodontal pathogens as well as the impact on the implant surfaces,so as to provide theoretical and experimental basis for PDT in periimplantitis therapy.Methods Artificial in vitro models were formatted by culturing Pg standard strain and ITI (International Team for Implantology) implants together in CDC broth.Then artificial in vitro models were treated by different doses of hematoporphyrin monomethyl ether (HMME) and different energy density of laser (EDL) for 60 s.The cultures were counted by colony form unit (CFU),and SPSS 17.0 statistical software was used for data statistical analysis to select the best EDL and HMME dose.Finally,ITI implants were observed by scanning electron microscope (SEM) to evaluate the impact of HMME-PDT on Pg of implant surfaces.Results When EDL was 12 J/cm2 and mass concentration of HMME was 25 μg/ml,SEM observations showed that PDT could effectively kill Pg ((13.00±5.00) CFU)without damaging the implant surfaces.Conclusions PDT therapy combining 630 nm diode laser with photosensitizer HMME have good bactericidal effect on Pg,and the EDL and HMME dose is as small as the clinical applicable safe dose.

Objective: To investigate the prevalence and influencing factors of dyslipidemia among residents in Chengdu City, so as to provide insights into improving the prevention and control of dyslipidemia. Methods: Based on the baseline survey of the Natural Population Cohort Study in Southwest China, residents aged 30 to 79 years was selected from 34 towns (communities) in 5 counties (districts) of Chengdu City using the multi-stage stratified cluster random sampling method in 2018. Demographic information and lifestyle behaviors were collected through questionnaires. Blood pressure, fasting blood glucose, serum uric acid, total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were collected through physical examination and laboratory tests. A multivariable logistic regression model was used to identify the factors affecting dyslipidamia. Results: A total of 21 113 participants were surveyed, including 9 331 males (44.20%) and 11 782 females (55.80%), and had a mean age of (50.80±12.32) years. The prevalence rate of dyslipidemia was 35.64%, and the prevalence rates of high TG, low-HDL-C, high TC and high LDL-C were 17.25%, 11.88%, 10.11% and 7.35%, respectively. Multivariable logistic regression analysis identified gender (male, OR=1.584, 95%CI: 1.463-1.716), age (50 to 79 years old, OR：1.221-1.444, 95%CI: 1.079-1.632), residence (urban, OR=1.123, 95%CI: 1.052-1.198), marital status (not married, OR=1.246, 95%CI: 1.128-1.376), educational level (high school and above, OR=0.914, 95%CI: 0.849-0.983), current smoking (OR=1.220, 95%CI: 1.121-1.327), drinking (1 to 2 d/week, OR=1.525, 95%CI: 1.368-1.700; 3 to 5 d/week, OR=1.857, 95%CI: 1.575-2.191; almost every day, OR=1.512, 95%CI: 1.269-1.801), sedentary time in leisure time (>2 h/d, OR=1.123, 95%CI: 1.046-1.206), central obesity (OR=2.212, 95%CI: 1.986-2.265), hypertension (OR=1.489, 95%CI: 1.388-1.598), diabetes (OR=1.998, 95%CI: 1.833-2.157) and hyperuricemia (OR=2.012, 95%CI: 1.848-2.192) as factors affecting dyslipidemia. Conclusion The prevalence of dyslipidemia among residents in Chengdu City was mainly associated with smoking, drinking, sedentary time, central obesity, hypertension, diabetes and hyperuricemia.