[Objective]To explore the feasibility and outcome of posterior single open-door and pedicle screw in treating canal stenosis and instability of cervical vertebrae at one time.[Method]The preoperative radiographs of 15 patients were measured and were diagnosed to be developmental spinal canal stenosis and instability of cervical vertebrae.MRI showed the spinal cords were compressed obviously.Posterior C_(3~7) single open-door decompression and canal-plasty was adopted.Self-designed pedicle screws and intertransverse fusion were used in the unstable segments.[Result]Complications were not found after the operation.The postoperative radiographs showed that the screws were in the right places.The average follow-up time was 13 months,ranging from 7 to 18 months.The short-term radiographs showed firmly fixed screws.[Conclusion]It is a simple and reliable method to treat canal stenosis and instability of cervical vertebrae at one time with posterior single open-door and pedicle screw.Anterior and posterior operations have been avoided and the short-term outcome is satisfied.

?Objective:To study the inductive effect of absorbable crystalline glass (ACG) and absorbable crystalline glass powder(ACGP) , in osteogenesis.Methods:Samples of ACG,ACGP and ? TCP in the size of 10 mm?3 mm?2.5 mm were prepared and implanted subperiosteally into the mandible deffects in 48 rabbits .Specimens obtained 2,4,12,and 24 weeks after implantation were observed with X ray film,measurement of area of crosssection energy dispersive X ray analysis(EDX),light and scanning electron microscopes.Results:Bone formation was found in 12 weeks and increased in 24 weeks in all implannts.The degradation (%) of ACG,ACGP and ? TCP in 24 weeks were 33,80 and 40 respectively.24 weeks after implantation the Ca ++ content (% of wt)in ACG,ACGP,? TCP and rabbit mandible were 20.54,20.29,19.96 and 19.71;the P ++ content (% of wt)10.54,10.51,10.72 and 10.96,respectively.Conclusion:ACG and ACGP are degradable,biocompatible and osteoinductive.

OBJECTIVE: To study the effect of insulin injection on the stability of cefodizime sodium for injection in 5% glucose injection.METHODS: The appearance and the pH value of the mixed solution in 12 h were monitored and the content of cefodizime sodium in the mixed solution was determined by HPLC.RESULTS: There were no evident changes for the mixture in appearance,pH value and the content of cefodizime sodium within 12 h at room temperature.CONCLUSION: Insulin injection has no effect on the stability of cefodizime sodium within 12 h at room temperature.

Objective The study consisted of two parts: in part Ⅰ the effects of propofol or/and procaine on CD18, CD62L expression and superoxide anion (SOA) release of phorbol 12-myristal 13-acetate(PMA) stimulated human neutrophils (PMNs) were studied in vitro; in part Ⅱ the effects of propofol or/and procaine on IL-6 and TNF-?production in endotoxin-stimulated human whole blood were studied. Methods PMNs were separated from the whole blood obtained from healthy 20-40yr old subjects. Part Ⅰ consisted of 9 groups: in group 1 (control) phosphate buffer solution was added to PMNs; in group 2 PMNs were stimulated by PMA 100mg?ml-1 ; in group 3-5 different concentrations of propofol (0.4, 4.0,40?g?ml-1) were added to PMA stimulated PMNs; in group 6-8 different concentrations of procaine (1.5,15,150?g?ml-1 ) were added to PMA stimulated PMNs; in group 9 propofol 2?g?ml-1 and procaine 8?g?ml-1 were added to PMA stimulated PMNs. Part II also consisted of 9 groups: in groupl whole blood was mixed with normal saline; in group2 lipopolysaccharide (LPS) l?g?ml-1 was added to whole blood; in group 3-5 different concentrations of propofol (0.4,4.0,40?g?ml-1) were added to LPS stimulated whole blood; in group 6-8 different concentrations of procaine (1.5, 15, 150?g?ml-1 ) were added to LPS stimulated whole blood; in group 9 propofol 2?g?ml-1 and procaine 8?g?ml-1 were added to LPS stimulated whole blood. CD18, CD62L expressions were measured by flow cytometry. SOA release was determined by cytochrome C reduction in the presence or absence of superoxide dismutase (SOD) . IL-6 and TNF-? production were measured by using radioimmunoassay. Results Propofol or/and procaine depressed CD18 up-regulation, CD62L shedding and SOA release of PMA-stimulated PMNs and propofol was more effective than procaine. Propofol enhanced but procaine inhibited the increased production of TNF-? and IL-6 in the LPS stimulated whole blood but when propofol and procaine were used in combination. The effectof procaine predominated. Conclusions Propofol or/and procaine can attenuate tissue damage induced by neutrophils by inhibiting the function of neutrophils.

Objective To investigate the clinical and pathological characteristics as well as immunophenotypes of subcutaneous panniculitis-like T-cell lymphoma for its differential diagnosis with other similar diseases.MethodsThe clinical、histological and immunophenotypic features of 6 cases were described in detail and related literatures were reviewed.Results All of 6 patients presented with subcutaneous nodules or /and erythematous plaques without lymph nodes swelling and with 5 cases had fever,one case developed to ulcer from its nodules.All of 6 patient presented typical histological changes and 2 of them associated with prominent hemophagocytic syndrome.The neoplastic cells were of T-cell phenotype.Two patients under went an aggressive clinical course with short survival period of 9～16 months and four patients who treated with chemotherapy have an improved survival state,but two of them had recurrence.Conclusion SPTCL is a uncommon type of T-cell lymphoma with clinical and pathological characteristics,and it needs to be differentiated from benign panniculitis or other lymphomas of the skin.

Objective To investigate the regulatory effects and possible mechanisms of autophagy induction and bactericidal activity in muring bone marrow derived macrophages ( BMDMs) . Methods Murine BMDMs were isolated and cultured in vitro. Then standard strain of E. coli was used to infect BMDMs and cellular autophagy levels and AMPK activation were detected. We next modulated functions of AMPK by pretreating cells with the specific agonist or antagonist of AMPK. Then cellular AMPK activation, autophagy levels and bactericidal activity were observed after E. coli infection. Results In E. coli infected BMDMs,autophagy related molecules like LC3B and Beclin1 were upregu-lated,accompanied with elevated LC3Ⅱ/Ⅰratio. Phosphorylation of AMPK was also upregulated by E. coli treatment. Enhanced AMPK activi-ty by its agonist leads to increased cellular autophagy and bactericidal activity,whereas inhibition of AMPK by its suppressor downregulated autophagy and dampened bactericidal activity. Conclusion AMPK and its related signaling pathway is required for anti-bacterial response in macrophage,which is dependent on its function in upregulating autophagy related molecules and inducing autophagy.

Objective To evaluate the clinical value of bedside echocardiography in full term infants in NICU.Methods The bedside echocardiography was performed for 313 full term infants,and then they were ana-lyzed.Results The main clinical characteristics of totally 313 cases of neonates were tachypnea,heart murmur and cyanosis.Patent foramen ovale and patent ductus arteriosus were common in the no cyanotic blood shunt,no cyanotic congenital heart disease(CHD)was mainly made of atrial septum defect(31 cases),followed by ventricular septal defect (20 cases).Cyanotic CHD was made of pulmonary atresia(6 cases),complete transposition of great artery (5 cases),tetralogy of fallot(5 cases),total anomalous pulmonary venous drainage(5 cases).Tricuspid regurgitation was common in abnormal blood flow,moderate to severe pulmonary hypertension was common for full term infants. Conclusion There were many abnormal echocardiography examination results,and atypical noise was common in neonatal CHD,routine examination of bedside echocardiography for full term infants in NICU was important for early detection of CHD and may help treatment timely to improve the prognosis.

Objective To investigate the effect of taurine on iron bioavailability in rats and its possible mechanism. Method SD rats were randomly divided into three groups:normal group (NG),taurine treated group (TG),experimental control group (EG). TG and EG were induced to be anemic experimentally for 4w before the study. Then the rats were fed iron supplements for 25 d,meanwhile TG was treated with taurine following hemoglobin(Hb) repletion bioassay. Regression analysis of TG and AG were measured following Hb repletion bioassay using iron supplements. And the relative biological value of TG following Hb repletion were calculated. The serum iron,unsaturated iron-binding capacity(UIBC),total iron-binding capacity(TIBC),transferin saturation(TS)and the expression of hepcidin were detected. Results The iron bioavailability of TG was higher than EG,and the RBV of TG was 126%. Other hematological variables of TG were close to NG,and better than EG. The expression of hepcidin was increased compared with NG,but decreased compared with EG. Conclusion Taurine can improve iron bioavailability in rats via suppressing hepcidin expression in rats.

Objective To explore the method for repair of total avulsion of 2~4 fingers and to find the best procedure. Methods The wrap-round flap with part toenail combind with the dorsal pedical flap to repair soft tissue defect of the dorsal of finger and part of the palmar of finger, the second simepulp flap to repair soft tissue defect of the palmar of finger,transfer of two tissues with one pedicle to repair total avulsion of 2~4 fingers. Results All of 9 composite transplants in 6 cases were survived,the received areas were primary healing in 8 finger,1 case repaired by the bilateral wrap-round flaps and second simepulp flaps for index and middle finger,the distal phalanx of middle finger necrosis because of dislocation of DIP,3 months later,injury healed after the necrosis of phalanx excised,but the nail and the nail bed were dead.Except 1 case which the grafted skin necrotized in the donor area of the big toe,the rest had primary healing.All cases were followed up 6~14 months,the activity of joints were: MP 0-70,PIP 0-40.The repaired pulps had excellent appearance,the nail was fine,1 finger's mail was atrophy and 1 finger's nail was defect.The donor area had good fountion without pain and edema,the grafted skin had normal color and the activity of joints were nomal. Conclusion The wrap-round flap with part toenail combined with the second simepulp flap is a good approach in treatment of the skin avulsion injury in the finger.

Objective:To investigate the effects of Tripterygium Wlilfordii on renal interstitial fibrosis and the expression of inflammatory factor in mice kidney with the Unilateral Ureteral Obstruction(UUO).Methods:Mice were randomly assigned to shame operation group(shame proup), UUO group, Tripterygium Wlilfordii and ACEI treatment group after UUO. The protein expression of ?-SMA,ICAM-1 were assessed by immunohistochemistry or Western blot; MCP-1 mRNA were detected by reverse transcription-polymerase chain reaction analyses(RT-PCR). And the change of renal pathology was observed at the same time.Results:In comparison with the shame group, the protein levels of ?-SMA, ICAM-1 and the mRNA expression of MCP-1 in UUO mice and treatment groups increased significantly, meanwhile Tripterygium Wlilfordii and ACEI could significantly reduce the expression of ?-SMA, ICAM-and MCP-1 on levels of protein and gene. The effects of Tripterygium Wlilfordii on ICAM-1 and MCP-1 were more significant than ACEI.Conclusion:Tripterygium Wlilfordii could reduce the transdifferentiation of renal interstitial cells and prevent renal interstitial inflammatory injury.