Intravenous thrombolysis has been proved as an effective therapy for acute ischemic stroke.However,symptomatic intracranial hemorrhage (sICH) occurred after thrombolysis will affect the recovery of patients,even endangers life when it is serious.Therefore,determining the risk factors for sICH after thrombolysis is helpful to select appropriate patients for thrombolytic therapy.The risk factors for slCH after thrombolysis include advanced age,male gender,obesity,higher National Institutes of Health Stroke Scale scores,hypertension,diabetes or higher blood glucose,atrial fibrillation,delayed thrombolysis time,antiplatelet therapy,anticoagulation therapy,and leukoaraiosis.However,some of the risk factors still have conflicting reports.Further large sample clinical studies are needed to confirm them.

The molding of humanistic spirit in medical colleges and universities has a direct bearing on whether or not the cultivated medical students can establish fundamentally the mental idea of humanistic solicitude and orientation of value.This article expounds the importance and urgency of strengthening the molding of humanistic spirit in medical colleges and universities,and analyzes the current situations of construction of humanistic spirit in colleges and universities of our country at present,and investigates the ways and countermeasures of strengthening the molding of humanistic spirit in medical colleges and universities.

Articular cartilage as a load-bearing connective tissues may often be caused defects by trauma,sports, degenerative or age-related diseases. As articular cartilage has a limited inherent repair capacity, a large variety of treatment approaches including autologous chondrocyte transplantation, microfracture and mosaicplasty methods have been developed. However, there are limitations in these strategies especially when using autologous chondrcytes. Mesenchymal stem cells are considered the most promising seed cells in the study of tissue engineered cartilage. This review focuses on the induction of mesenchymal stem cells into chondrocytes in vitro and their application in cartilage repair.

The porcine reproductive and respiratory syndrome virus (PRRSV) can cause reproductive barriers in breeding pigs and respiratory symptoms in piglets. In this review, we summarize research progress of the infection and replication mechanisms of the PRRSV. We also review the virulence determinants of the PRRSV. All these fundamental studies are important for the control and elimination of the PRRSV.
Animals ; Porcine Reproductive and Respiratory Syndrome ; virology ; Porcine respiratory and reproductive syndrome virus ; genetics ; pathogenicity ; physiology ; Swine ; Virulence ; Virus Replication

Autophagy is a process for the degradation of long half-life proteins and organelles via the lysosome system. A large body of evidence indicates that the autophagic phenomena exist in the progressive atherosclerotic plaques. Vascular smooth muscle cells, macrophages, and endothelial cells treated with some stimulants that result in atherosclerosis formation in in vitro experiments, these cells show certain autophagic features, such as myelin-like structures, accumulation of ubiquitinated inclusions in the cytoplasm, and extensive vacuolization. However, although the interests in research on autophagy are increasing, the exact role of autophagy in atherosclerosis remains unclear. Therefore, understanding the underlying cellular and molecular mechanisms of autophagy will provide a new idea for studying the mechanisms and treatment of atherosclerotic disease.

Objective To evaluate our early results of a combined minimally invasive surgery with surgical procedures for critical extremity ischemia. Methods From July 1999 to October 2000 intraluminal iliac minimally invasive surgery combined with simultaneous conventional surgery were performed on 20 lower extremities of 15 pa- tients suffering from critical multilevel atherosclerotic occlusive disease. There were 11 men and 4 women with an aver- age age of 73 years. The indications for surgery included disabling claudication in 6, rest pain in 9(included tissue gan- grenes in 3)patients. Mean preoperative ankle - brachial index(ABI)was 0. 35 ? 0. 18. Results 17 iliac mini mal- ly invasive approaches combined with simultaneous 11 femoro - popliteal bypass and 3 femoro - femoral popliteal by- pass as well as 5 profundaplasty were performed in 20 limbs of 15 patients. minimally invasive surgery were technically successful in all patients. One femoral - popopliteal bypass was failure. There were no additional instances of procedural or postoperative morbidity or mortality, and no wound - related complications. Postoperative ABI of femoral - popliteal bypass was 0.74 ? 0. 16(p

Purpose:To explore the relationship between the expression of nm23mRNA,p16 and the oncogenesis,development and metastasis of lung cancer.Methods:Expressional level of nm23mRNA,p16 was detected in 72 human lung cancer samples and 23 normal tissues adjacent to cancer respectively,and their correlation with lymph node metastasis and the degree of cell differentiation was studied by immunohistochemistry (IHC) and in situ hybridization (ISH) methods on tissue microarray.Results:The positive expression for nm23mRNA in cancer samples,in human tissues adjacent to cancer and in primary cancer with lymph node metastasis was 55.6%(40/72),82.6%(19/23),25.0%(6/24) respectively.The positive expression for p16 was 45.8%(33/72),73.9%(17/23),25.0%(6/24) respectively.The expression level of nm23mRNA and p16 were closely related to the degree of cell differentiation( P 0.05).Conclusions:These results suggest that nm23 gene and p16 gene are closely related to the degree of cell differentiation and lymph node metastasis of lung cancer.The lower expression level of nm23mRNA and p16 predicts the metastasis of lung cancer, and the two genes may be an index in monitoring the status and evaluating the prognosis of the lung cancer.

0.05).There are significant statistical differences in NIHSS(91.3% vs 71.3%)and BI(93.8% vs 75.0%)between two groups af- ter treatment(P0.05). Conclusion Multidisciplinary integrated treatment mode is an effective treat ment for stoke.

OBJECTIVE:To systematically review the efficacy of isotretinoin in the treatment of acne vulgaris,and to provide evidence-based reference for clinical treatment. METHODS:Retrieved from PubMed, Cochrane Library, Wanfang, VIP and CJFD,randomized controlled trials (RCT) about isotretinoin (test group) versus viaminati,macrolides,tetracyclines antibiotics and other drugs (control group) in the treatment of acne vulgaris were collected. Meta-analysis was performed by using Rev Man 5.2 software after data extract and quality evaluation by Cochrane 5.1.0. RESULTS:Totally 28 RCTs were enrolled,involving 3 534 patients. Results of Meta-analysis showed,the total effective rate of isotretinoin was significantly higher than viaminati [RR=1.56, 95%CI(1.27,1.91),P<0.001],macrolides[RR=1.44,95%CI(1.31,1.58),P<0.001] and tetracyclines antibiotics [RR=3.05,95%CI(2.29,4.07),P<0.001];improvement of skin lesions scores was significantly better than control group [MD=0.33,95%CI(0.04, 0.62),P<0.05],the differences were statistically significant. Dry lips,cheilitis,dry skin and nasal mucosa and pruritus were the main adverse reactions,and no serious adverse reactions were showed. CONCLUSIONS:Isotretinoin has better efficacy and safety than viaminati,macrolides and tetracyclines antibiotics.

Objective To explore the effect of microRNA‐21(miR‐21) on the migration and invasion ability in human laryn‐geal squamous carcinoma cell Hep2 .Methods The MTT method was used to detect the viability of Hep2 cells at 48 h after miR‐21 inhibitor and miR‐21 NC transferring into Hep2 cells by LipofectamineTM 2000 .The cell migration ability was detected by using the scratch test .The cell invasion ability was detected by using the Transwell method .The activation of phosphatase and tensin homo‐logue deleted on chromosome 10 (PTEN)/phosphatidylinositol 3 kinase (PI3K) /protein kinase B(Akt) signal pathway and the expression of matrix metalloproteinase 2 (MMP2) ,MMP9 ,reversion inducing cysteine rich protein with kazal motif (RECK) was detected by using the Western blotting .Results Compared with miR‐21 NC ,miR‐21 inhibitor could significantly reduce the Hep2 cellviability[(0.688±0.043)vs.(0.375±0.012)],inhibitedthemigrationability[(6.57±0.02)μm vs.(20.49±2.18)μm]and invasion ability[(100 .7 ± 10 .2) vs .(46 .8 ± 4 .3)] ,and the differences were statistically significant (P<0 .01) ,meanwhile miR‐21 inhibitor could down‐regulate the expression of PI3K ,MMP2 and MMP9(P<0 .01) ,and reduced the phosphorylation level of Akt (P<0 .01) ,up‐regulated the expression of PTEN and RECK (P<0 .01) .Conclusion miR‐21 inhibitor can significantly suppress the migration and invasion ability of Hep2 ,which may be related with the PTEN/PI3K/Akt signal pathway .