OBJECTIVE: To study the effect of Qi-replenishing and Yin-nourishing in inhibiting tumor metastasis. METHODS: Fifty mice were randomly divided into replenishing Qi group, replenishing Yin group, replenishing Qi and Yin group, chemotherapy group and control group. Models of Lewis lung cancer and B(16) melanoma were observed in vivo. The tumor weight inhibiting rate, metastatic lung tumor, concentrations of t-PA and TXB(2) in tumor tissue and IL-2 and TNF-alpha in spleen were observed. RESULTS: The tumor weight inhibiting rates of replenishing Qi and Yin group for Lewis lung cancer and B16 melanoma were both 42.9%. Replenishing Qi and Yin could decrease the contents of TXB(2) and 6-keto-PGF(1alpha), and increase the levels of IL-2 and TNF-alpha. CONCLUSION: Replenishing Qi and Yin can promote the healthy energy to inhibiting tumor metastasis.

Objective To investigate the effect of different dose dexmedetomidine on reducing agitation after sevoflurane anesthesia in patients underwent repair of indirect inguinal hernia.Methods 105 cases of ASA Ⅰ ～ Ⅱ received operation with inhalation of 3％ sevoflurane to induce anesthesia,and then randomly divided into three groups,35 cases in each group.The control group injected with 0.9％ sodium chloride,observation 1 group injected dexmedetomidine 0.15μg/kg,observation 2 group injected dexmedetomidine 0.30μ g/kg.Then placed oropharynx airway ventilation,caudal block.Anesthesia was maintained with 1.2％ sevoflurane,maintain its independent ventilation.Hemodynamic and respiratory changes of the children were recorded.Time to eye opening(TEO) at the end of anesthesia was also recorded.Results Three groups of children had similar operation process.TEO of the control group was (8.45 ± 4.02) min,observation 1 group was (9.23 ± 5.03) min,observation 2 group was (10.81 ± 4.06) min (t =1.23,1.64,all P ＞ 0.05).The incidence rate of agitation of the control group was 42.8％,that in the observation 1 group and observation 2 group was 20％,8.6％.Observation 1 group was significantly lower than that of control group (x2 =3.72,P ＜ 0.05),observation 2 group was significantly lower than that of control group and observation 1 group (x2 =5.14,4.27,all P ＜ 0.05).Conclusion The dose of dexmedetomidine 0.3 μg/kg administered after induction of anesthesia could reduce the postsevoflurane agitation in children and have no adverse effects.

The present study used the experimental patterns of Go/No Go and no motion contingent negative variation (CNV) task into the research in order to study whether the CNV can express the implication of expectation. Through comparing the CNV under different conditions, the data collected from experiment showed that the key to evoked CNV was close to the warning signal and command signal. Whether the command signal was related to the task would impact on the amplitude of the CNV. This characteristics responses to the subjects' expectation. On this basis, CNV can be used as the electrophysiological index for the reflection of expected value in the conditions of this experiment.
Anticipation, Psychological ; Brain Waves ; Contingent Negative Variation ; Humans

Objective To investigate the therapeutic effect of island flap pedicled with dorsal cutaneous branches of digital inter-nal artery for severe thermos injury of fingers .Methods From May 2008 to May 2012 ,15 fingers in 12 cases with severe thermos injury were treated with island flap pedicled with dorsal cutaneous branches of digital internal artery .The defect size ranged from 1 .5 cm × 2 .0 cm to 2 .2 cm × 3 .0 cm ,4 fingers with anterograde flap repair ,11 fingers with retrograde flap repair .Results Partial necrosis happened at the distal end of 2 island flaps ,All the other flaps survived completely .The patients were followed up for 6-12 months with satisfactory cosmetic and functional results ,cicatricial contracture around the skin graft in 3 fingers ,The two-points discrimination distance was 9-11 mm in flaps .Conclusion These flaps have the advantages of an extended skin paddle and a versa-tile pivot point on the phalanx ,and they allow coverage of wide and distal defects .

The effect of cigarette smoke extract (CSE) on the proliferation of human airway epithelial cells and the possible mechanism was studied. After airway epithelial cells were treated with different concentrations of CSE for 24 h, the cell proliferation was measured by MTT and the distribution of different cell cycles by flow cytometry. The FAK expression level was detected by Western blot and the degree of tyrosine phosphorylation by immunoprecipitation. The results showed that CSE could inhibit the proliferation of human airway epithelial cells, arrest the epithelial cells in G1 phase of cell cycle, dramatically decrease the number of epithelial cells in S and G2 phases; Meanwhile CSE could decrease the expression level of FAK and the degree of its tyrosine phosphorylation. The above effects of CSE were concentration-dependent. The expression of FAK and the degree of its phosphorylation was positively correlated to the increased number of epithelial cells in G1 phase, and negatively to the number of epithelial cells in S and G2 phases. It was concluded that the mechanism by which CSE could inhibit the proliferation of human epithelial cells was contributed to the increased expression and activation of FAK.
Bronchi/*cytology ; Bronchi/metabolism ; Cell Cycle/drug effects ; Cell Proliferation ; Cells, Cultured ; Enzyme Activation ; Epithelial Cells/*cytology ; Epithelial Cells/enzymology ; Focal Adhesion Protein-Tyrosine Kinases/biosynthesis ; Focal Adhesion Protein-Tyrosine Kinases/*metabolism ; Phosphorylation ; Tobacco/adverse effects ; Tobacco Smoke Pollution/*adverse effects

To rapidly identify the compound Yiganling tablets from different manufacturers by near-infrared fibre-opti-cal spectroscopy and detect the content of silymarin. Methods:The ninety-three batches of compound Yiganling tablets from six manu-facturers were used as the analysis objects. Clustering analysis model of compound Yiganling tablets was established. The spectra were pretreated with the second derivative and vector normalization;the number of smoothing point was thirteen; the wavelength range was 9 000-4 100 cm-1;the distance between the spectra was Euclidean distance, the distance between the spectra and the class was the sum of square of the variance(Ward’s method). The quantitative model was established using the same samples. The spectra were pretreated with the first Derivative and vector normalization;the number of smoothing point was seventeen;the wavelength ranges were 9 100-7 300 cm-1 and 7 100-4 100 cm-1;the regression method was partial least squares ( PLS) algorithm. Results:The clustering model could identify the samples of training set, and validate the thirty spectra of test set accurately. For the quantitative model of the content of silymarin, the root mean square error of cross validation (RMSECV) was 0. 082 8 and the determination coefficient R2 was 97. 98%. The root mean square error of prediction (RMSEP) was 0. 044 1 and the correlation coefficient R2 was 98. 95%. Conclu-sion:The clustering analysis and the quantitative model are rapid and simple, accurate and reliable, and can be applied in fast drug a-nalysis.

Interleukin 24(IL 24),also called the melanoma differentiation associated gene 7(MDA 7),is stably expressed in human tissues associated with the immune system such as the spleen,thymus,peripheral blood leukocytes and normal melanocytes. IL 24 binds to IL 20 and IL 22 receptor complexes(IL 22R1/IL 20R2 and IL 20R1/IL 20R2),and induces secretion of high levels of IL 6,TNF alpha,and IFN gamma and low levels of IL 1beta,IL 12,and GM CSF from human PBMC. Adenoviral IL 24(Ad IL 24) induces growth suppression and apoptosis selectively in diverse human cancers and tumor cell lines without producing any apparent harmful effect in normal cells. The effects of Ad IL 24 are associated to the ratio of pro apoptotic(BAX,BAK) to anti apoptotic(Bcl 2) proteins,and the up regulation of p38 MAPK and a family of growth arrest and DNA damage(GADD) inducible genes. These demonstrate the potential therapeutic effects of Ad IL 24 in human cancer.

Objective To investigate the application value of amniotic cell culture and karyotype analysis in prenatal diagnosis . Methods 1 016 pregnant women in second trimester were subject to amniocentesis under the guidance of B-type ultrasonic inspec-tion ,and the cell culture and karyotype analysis were performed on the amniotic fluid which had been drawn out .Results Among 1 016 pregnant women ,1 011(99 .5% ) succeeded in the first operation of amniocentesis and cell culture .The detection rate of fetal chromosomal abnormal karyotype was 10 .3% (105/1 016) ,in which 8 .3% (85/1 016) of structural abnormality and 2 .0%(20/1 016) of quantity abnormality .856 cases were received follow-up .Conclusion Amniotic fluid cell culture and karyotype analy-sis is a safe and reliable method for prenatal diagnosis .

Objective To study the effect of clomiphene citrate (CC) and human menopausal gonadotropin (hMG) on the expression of integrins ? 4? 1 during implantation window in endometrium. Methods Endometrium integrin ? 4? 1 expression was detected by immunohistochemistry method in the mid secretory phase of 48 normal natural cycles. CC+hCG or CC+hMG+hCG cycles in 30 polycystic ovary syndrom (PCOS) patients and 48 normal women. Results The expression of endometrial integrin ? 4? 1 was stronger in the natural cycls than those of ovulation induction cycles with CC+hCG or CC+hMG+hCG. So was the pregnancy cycles as compared with the non pregnant cycles. Conclusion The endometrial receptivity in the min luteal phase and pregnant rate declined during ovulation induction by CC+hCG or CC+hMG+hCG in either normal women or PCOS patients.

Objective To investigate the water source contamination in Jiangsu Province. Methods Using blowing and arresting device and GC-MS, 90 water samples collected from 15 city drinking water sources in Jiangsu Province were analyzed. Results The main detected compounds were 1, 2-dichloroethane, chloroform, benzene, trichloroethene, toluene, tetrachloroethene, chlorobenzene, m-xylene, p-xylene, o-xylene, 1, 4-dichlorobenzene, 1, 2-dichlorobenzene, 1, 2, 4-trichlorobenzene and hexachlorobutadiene, while 1, 1-dichloroethene, trans-1, 2-dichloroethene, cis-1, 2-dichloroethene, 1, 1, 1-trichloroethane, carbon tetrachloride, bromodichloromethane, dibromochloromethane, bromoform, isopropylbenzene, 1, 2, 3-trichlorobenzene and styrene were not found. The highest concentration of 1, 2-dichloroethane was 27.79 ?g/L, which was close to the limit of surface water. It should be noticed that the detected concentrations of chlorobenzene in water source of district 3 and water source of district 4 were a little higher. Compared with the others, in water source of district 1, water source of district 9 and water source district of 14 a higher concentration of toluene and xylene were detected and the concentration of benzene in water source of district 1, water source of district 2, water source of district 3 and water source district 4 was higher. Only in water source of district 13, none of 25 volatile organic compounds was detected. Conclusion Some drinking water sources have been contaminated by volatile organic compounds in Jiangsu Province.