1.Advance in methods of induced-embryonic stem cell differentiation to retinal cells
Chinese Journal of Experimental Ophthalmology 2016;34(9):851-854
Retinal degenerative disease is the leading cause of visual loss,the mechanism is not clear and has no effective treatment method.In recent years,the field of stem cell research has made great progress.Stem cells have the potential of differentiating into all the body cells.Embryonic stem cells (ESCs) can be used to differentiate a variety of retinal cells,which has brought a new promise for the treatment of retinal degeneration disease.However,the most important step of this treatment is how to differentiate ESCs into photoreceptor cells and retinal pigment epithelium (RPF) cells.In this paper,we introduced the recent progress in the differentiation methods of ESCs into retinal cells,which contains spontaneous differentiation method,co-culture method,growth factors and small molecules induced method,adherent monoculture method,three dimensional differentiation method.
2.The effects of intravesical therapy with elemene in preventing postoperative recurrence of bladder cancer
Chuangang LI ; Yongji LIU ; Zhilu FAN
Chinese Journal of Urology 2001;0(04):-
Objective To investigate the effects of intravesical therapy with elemene in preventing postoperative recurrence of superficial bladder cancer and its toxic and side effects. Methods This series included 123 patients with superficial bladder cancer (T 1),consisting of transitional cell carcinoma GⅠ in 37 cases,GⅡ in 73 and GⅢ in 13.They all underwent surgical treatment. Postoperatively, they were randomly assigned to 2 groups:63 patients in elemene group received instillation of elemene (400 mg,once a week) 2 weeks after operation and 60 patients in mitomycin C (MMC) group received instillation of MMC(40 mg,once a week) 2 weeks after operation. The instillations were repeated for 6 weeks and thereafter monthly for 1 year.The recurrence rates,side effects,and NK cell activity before and after treatment were evaluated. Results The recurrence rate of elemene group (mean follow-up of 19.7 months) was 7.9% (5 cases),which was significantly lower than that (25.0%,15 cases) of MMC group (mean follow-up of 19.4 months;P
3.Effect of antisense phosphorothioate oligonucleotide on activity of telomerase for human bladder tumor cell
Zhiyu LIU ; Yongji LIU ; Yuyan LI
Journal of Clinical Surgery 2002;0(S1):-
Objective To detect the Telomerase inhibitor-antisense phosphorothiate oligonucleotide (asONS) effect on telomerase activity of human bladder tumor cell line BIU-87.Methods BIU-87 cells and cell extracts are cultured with asONS,non-asONS and control fluid at different concentrations and times respectively.Telomerase activity was assayed by TRAP PCR-ELASA.Results AsONS can inhibit the activity of BIU-87 cells ,whereas non-asONS and control group not, and have significant difference(P
4.Determination of deoxyschizandrin and schisandrin B in Liganlong Tablet by HPLC
Shaowa LV ; Qingxia GUAN ; Yongji LI
Chinese Traditional Patent Medicine 1992;0(05):-
AIM: A quantitative method was developed for the determination of deoxyschizandrin and schisandrin B in Liganlong Tablet(Fructus Schisandrae Chinensis,Radix Astragali,Radix Angelicae Sinensis,Radix et Rhizoma seucaulis Acanthopanacis Senticosi,etc.) by HPLC. METHODS: The chromatographic conditions included column Symmetry shield~(TM) RP_(18) 5 ?m,3.9 mm?150 mm,mobile phase: methanol-tetrahydrofuran-water(64∶4∶32),flow rate at 1 mL/min,wavelength at 220 nm. RESULTS: The number of theoretical plates is 3555.5.The Deoxyschizandrin liner is 0.068~0.340 ?g (r=0.999 9) and the Schisandrin liner range is 0.06~0.30 ?g (r=0.999 8).The resolutions are 5.09,1.12,respectively. CONCLUSION: The method is sensitive,quick and accurate for the determination of deoxyschizandrin and schisandrin B in Liganlong Tablet.
5.Influence of escin on the renal function of BXSB mice
Yongji LI ; Anbin HUANG ; Xingruo ZENG
Chinese Journal of Rheumatology 2008;12(10):690-692
Objective To study the effect of escin on proteinuria and renal function of BXSB mice. Methods BXSB mice were divided randomly into group A (control group), B (steroid treatment group), C(steroid combined with high-dose esein treatment group) and D (streroid combined with low-doze escin group). The proteinufia and renal function were detected by albustix and automatic biochemistry analyzer after a month's treatment. Results Group C reduced the level of proteinuria and parameters of renal function (BUN and Cr) significantly when compared with other three groups (P<0.05). Conclusion Escin can reduce the level of proteinuria and protect renal function of BXSB mice.
6.Content Determination of Sinomenine in Sinomenine External Applied Powder by UPLC
Rui WANG ; Chenxue LI ; Yue HU ; Yuyan GUO ; Yongji LI
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(3):89-90,91
Objective To establish an UPLC method for the determination of sinomenine in Sinomenine External Applied Powder. Methods The UPLC method was carried out on a C18 column by using acetonitrile-water-ethylene diamine (50:50:0.25) as mobile phase. The flow rate was 0.2 mL/min; the sample quantity was 2 μL; the detection wavelength was 283 nm. Results The peak time was within 1 min or so. The calibration curve of sinomenine was in the linear range of 34.2–2188.0 ng. Conclusion The method is simple, rapid, stable and reliable, which can be used for the determination of sinomenine in Sinomenine External Applied Powder.
7.Pseudo-color enhancement in calcium sparks detection of skeletal muscle fiber
Changhai LEI ; Yongji YANG ; Dongfang LI ; Zhinian ZHU
Chinese Medical Equipment Journal 2004;0(07):-
Fluorescence probe Fluo-3AM can be luminary in confocal microscope after combining Ca2+,So nomadic Ca2+ in cytoplasm can be measured and tested by this way.Some confocal laser scan images are obtained after calcium in muscle fibers signed with F1uo-3AM. Then mapping function is used to convert the gray level image into colorful image by pseudo-color enhancement theory in digital image.Compared to the original gray level image,the processed pseudo-color image could be more easily distinguished.So the methods had a great value in Ca2+ detection fields.
8.Method of calling MATLAB in VB application based on ActiveX
Changhai LEI ; Yongji YANG ; Weiwei SUN ; Dongfang LI
Chinese Medical Equipment Journal 2003;0(11):-
Objective To discuss the method of calling MATLAB in VB application program based on ActiveX.Methods MATLAB provided automatic service support to ActiveX,and MATLAB could be called as ActiveX of interpreter through ActiveX automatic interface.Results Good effect was achieved when this method was applied to noise filtering of patch clamp.Conclusion By using this method,users can embedded mix programming in self-developed application software conveniently,thus reducing the difficulties in programming.
9.Research on synchronous control by remote apparatus based on Windows API
Changhai LEI ; Yongji YANG ; Zhinian ZHU ; Dongfang LI
Chinese Medical Equipment Journal 2004;0(08):-
This paper gives the techniques and steps of creating remote control instruments system using Windows API functions,and explains its hardware and software structures.The whole system is proved to be stable and reliable by experiment.It can provide a new experimental method for biomedical research,which enable the construction of multifunctional experiment platform.
10.Signal noise filtering of patch clamp based on Wavelet transform
Changhai LEI ; Yongji YANG ; Dongfang LI ; Leping ZHANG
Chinese Medical Equipment Journal 2003;0(11):-
Wavelet transform(WT) is a powerful technique in signal separation and can improve the signal-to-noise ratio by separating the white noise and useful signal.The method in the paper introduces the concepts of Wavelet transform.According to the analysis of Wavelet coefficients between the noise and signal at different levels,the appropriate filter is realized.The patch clamp experiment results demonstrate the feasibility of the method.