AIM:To establish a HPLC method for simultaneously determining chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin in Shuanghuanglian Powder for Injection(Flos Lonicerae japonicae,Fructus Forsythiae,Radix Scutellariae,etc.).METHODS:HPLC assay was carried out on a Phenomenex Luna C_ 18 column(250 mm?4.6 mm,5 ?m).The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set as multi-wavelength detection.RESULTS:The linear ranges of chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin fell within the range of 0.204-2.04 ?g、0.010 2-0.102 ?g、0.186-1.86 ?g、0.058 5-0.585 ?g and 2.52-25.2 ?g,respectively.The five components showed good linear correlations(r=0.999 7、0.999 4、0.999 8、0.999 8、0.999 9).The average recoveries of five components were in accordance with the determination requirements.CONCLUSION:The method is simple,quick,reliable,accurate and very suitable to the quality control of Shuanghuanglian Powder for Injection.

AIM: To establish a HPLC method for simultaneously determining chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin in Shuanghuanglian Powder for Injection (Flos Lonicerae japonicae,Fructus Forsythiae,Radix Scutellariae,etc.).METHODS: HPLC assay was carried out on a Phenomenex~((R)) Luna C_(18) column (250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.2% phosphoric acid solution as gradient elution.The flow rate was 1.0 mL/min.The detection wavelength was set as multi-wavelength detection.RESULTS: The linear ranges of chlorogenic acid,caffeic acid,forsythin,forsythoside A and baicalin fell within the range of 0.204-2.04 μg ,0.010 2-0.102 μg,0.186-1.86 μg,0.058 5-0.585 μg and 2.52-25.2 μg,respectively.The five components showed good linear correlations ( r = 0.999 7,0.999 4,0.999 8,0.999 8,0.999 9 ).The average recoveries of five components were in accordance with the determination requirements.CONCLUSION: The method is simple,quick,reliable,accurate and very suitable to the quality control of Shuanghuanglian Powder for Injection.

Objective To observe the efficacy and safety of endoscopic ultrasonography submucosal resection for treatment of gastric muscularis propria mesenchymal tumor.Methods Endoscopic ultrasonography preliminary diagnosis of 19 cases were diagnosed stromal tumors originating from gastric muscularis propria layer,the endoscopic ultrasonography diagnosis the source,echo,size,blood supply,etc.than treated by endoscopic submucosal excavation.To observe the therapeutic effect and complications.Results Eighteen patients were the full excavation,1 patient was failed.Intraoperative perforation was seen in 2 cases,the wound was healed by the titanium clamp.A small amount of bleeding happened in operation and bleeding was stopped by hot biopsy forceps or argon coagulation.No delayed bleeding happened.Conclusion Submucosal resection for gastric muscularis propria mesenchymal tumors is safe and effective,endoscopic ultrasonography has important value in the strict assessment.

Objective To optimize the formulation of the Garlic oil ?-CD sustained-release tablet. Methods The preparation formulation of the sustained-release tablet was optimized by orthogonal design which took the released rate as the index. Result The optimized formulation contained 9% HPMC, 15% lactose, 10% PVP, 10% microcrystalline cellulose. The ingredient of Garlic oil sustained-release tablet by this formulation was released slowly in 8 h. Conclusion The optimized formulation is rational and stable, the tablet could be released slowly.

Objective To determine the total sodium in Danshen for injection.Methods Flame atomic absorption spectroscopy was used to determine the total sodium in Danshen for injection.Results The calibration curves were linear in the range of 0.5～10 mg/L for sodium chloride.The average recovery rates of sodium chloride were 101.8%,RSD=1.60%.Conclusion This method was found to be simple,quick and accurate for simultaneous determination of sodium in Danshen for injection.

Objective To establish a determination method of Allicin in Garlic oil Sustained-release Tablets. Method Diamonsil C18 column was used with a mobile phase of mathanol-water-formic acid (80∶20∶0.1). The detective wavelength was at 218 nm. The flow rate was 1.0 mL/min. Column temperature was at 25 ℃. Results Allicin showed a good linear relationship at the range of 0.05～0.25 ?g (r=0.999 9). The average recovery rate was 99.5% (RSD=1.7%). Conclusion The method is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Allicin in Garlic oil Sustained-release Tablets.

Objective To optimize the extraction process of Yangwei Jinshi Granules. Methods An orthogonal test was adopted in the study. With the paste-forming rate, the content of extract and the content of hesperidin of Yangwei Jinshi Granules as indices, data processing was carried out with grading method of synthesizing multiple indexes for optimizing the extraction condition. Results The best extraction technology was as follows:boiled 2 times, first with 10 times amount of water for 1.5 h, and then boiled with 8 times amount of water for 1 h. Conclusion The optimized process is reasonable, stable and economic for Yangwei Jinshi Granules.

Objective To establish a method of HPLC gradient elution method determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules. Methods Kromasil C18 column was used with a mobile phase of acetonitrile-water gradient elution, column temperature at 25 ℃. Results Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1 showed good linear relationship at the range of 0.495～1.782 ?g (r =0.9999), 2.65～9.54 ?g (r =0.9995), 1.85～6.66 ?g (r =0.9999), respectively. The average recovery:Notoginsenoside R1 was 100.60% (RSD=1.99%), Ginsenoside Rb1 was 100.86% (RSD=2.19%) and Ginsenoside Rg1 was 98.83% (RSD=1.95%), respectively. Conclusion The methods is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules.

Objective To evaluate the safety and possibility of coronary intervention in uremic patients on dialysis. Methods Three uremic patients with unstable angina pectoris were treated successfully with percutaneous transluminal coronary angioplasty and stenting. In order to minimize the adverse effect on kidney due to contrast, non-ionic and low osmolar contrast medium was used and coronary angiography and intervention therapy were performed separately. Further protection of the renal function was effected by more frequent dialysis and increasing fluid administration. Results Coronary contrary shows the stenosis of multiple vessels in one patient, unique vessel lesion in two patients. One to four stents were placed. Clinical follow-up period of 12-18 months after procedure, angina pectoris disappeared in all three patients and no deterioration of renal function was noted. Conclusion It is possible to perform coronary angiography and stenting in uremic patients on hemodialysis successfully and safely, provided due attention was paid to the choice of contrast medium and protective measures for renal function.

Objective To describe the early use of nasal continuous positive airway pressure (nCPAP) ventilation for infants presenting acute congestive heart failure (CHF) complicated by congenital heart disease (CHD) and pulmonary artery hypertension (PH).Methods Sixty infants with CHD treated for acute CHF were randomly divided into the nCPAP group (n =32) and the non-nCPAP group (n =28).Data were analyzed,which included lactic acid value (Lac) by arterial blood gas analysis,calculation of oxygenation index [pa (O2)/FiO2],detection of serum N-terminal pro-brain natriuretic peptide of type B (NT-proBNP) level,determination of left ventricular end diastolic volume index (LVEDVI),left ventricular ejection fraction (LVEF) and pulmonary artery systolic pressure (PASP) by noninvasive bedside ultrasonic cardiogram (UCG).Results 1.Comparison of arterial blood gas between the 2 groups:1 d after treatment,there were significant differences in pa (O2)/FiO2 and Lac between the 2 groups (t =4.743,5.402,all P ＜ 0.05).A significant difference was found in the pa (O2)/FiO2 between the nCPAP group and the non-nCPAP group 3-7 d after treatment(t =6.366,P ＜ 0.05).The level of Lac had no significant difference between the 2 groups 3-7 d after treatment(t =1.812,P ＞ 0.05).2.Comparison of index of heart function between the 2 groups:after 3-7 d treatment,LVEDVI,LVEF,and PASP were statistically different between the 2 groups (t =2.052,2.704,2.019,all P ＜0.05).3.Comparison of serum indexes between the 2 groups:3-7 d after treatment,serum NT-proBNP level was improved dramatically compared with the non-nCPAP group(t =9.869,P ＜0.05).4.Comparison of clinical prognosis between the 2 groups:the differences in needing endobronchial intubation rate,mechanical ventilation time,time in PICU and mortality rate were all statistically significant between the 2 groups (x2 =5.505,P =0.019; t =4.788,P =0.000;t =5.068,P =0.000 ;x2 =4.284 ;P =0.038).Conclusions The early use of noninvasive nCPAP for eligible patients with acute CHF complicated by CHD and PH seems to improve their prognosis by improving pa (O2)/FiO2,reducing left ventricular and right ventricular afterload and improving the left ventricular function.