Objective To investigate the imaging diagnostic value of the early avascular necrosis of the femoral head(ANFH) in adult.Methods There were 25 cases (34 hips) with early ANFH diagnosed by imaging and clinical data.Radiography,CT and MRI findings of ANFH were analysed comparatively.Results In the 34 ANFH included stage Ⅰ 13 hips,stage Ⅱ 21 hips.The diagnostic accurary was 32.4% for X-ray,61.8% for CT and 100% for MRI.Conclusion MRI is better than the other technique in early finding the lesions of ANFH,and the diagnostic sensitivity and accuracy of MRI are higher than that of CT and X-ray.

Objective: To investigate the effect of zinc sulfate and zinc methionine on growth and their possible regulating mechanism in mice. Method: Ninety male KM mice were randomly divided into three groups. The control group was fed on basal diet containing zinc of 11. 67 mg/kg 10d. The ZnSO4 group and Zn-Met group were fed on the diets supplemented with ZnSO4 or Zn-Met at 30 mg/kg(on the basis of Zn) for 10 d. Initial and final body weight,serum zinc concentration, growth hormone (GH),the levels of growth hormone receptor (GHR) and insulin like growth factor 1 (IGF-1) mRNA were determined. Results: Both ZnSO4 and Zn-Met enhanced body weight and serum zinc concentration of mice,Zn-Met more effectively than ZnSO4 for body weight . Both forms of zinc had no effect on GH and the expression of GHR mRNA , but both up-regulated the expression of IGF-1 mRNA. As compared to ZnSO4, Zn-Met enhanced the level of IGF-1 mRNA significantly. Conclusion: Both ZnSO4 and Zn-Met had no effect on GH and the expression of GHR Mrna,but enhanced the expression of IGF-1 mRNA. Zn-Met enhanced the body weight gain and up-regulated IGF-1 mRNA expression more effectively than ZnSO4.

Objective:To investigate the antihypertensive effects of mung bean protein,peanut protein and rice protein alcalase hydrolysates with in vitro angiotensin I-converting enzyme(ACE) inhibitory activity in spontaneously hypertensive rats(SHR) . Method:The impact of digestive proteases on ACE inhibitory activity of hydrolysates of peanut,mung bean and rice protein isolates were evaluated under simulated gastrointestinal digestion and their antihypertensive effects were investigated in SHR after single oral administration. Results:All of three kinds of protein hydrolysates showed antihypertensive activities after single oral administration at a dose of 600 mg/kg bw,most potent in mung bean protein while least in peanut protein. There were no significant changes in the heart rate of SHR after oral administration of protein hydrolysates. Conclusion:Mung bean protein,peanut protein and rice protein hydrolysates all showed antihypertensive activity,but their potent inhibitory activities on ACE did not correlate with their antihypertensive activities found in SHR.

Objective:To study the protective effects of exogenous nucleotides on lipopolysaccharide(LPS)immunnostimulation and its mechanism.Method:Forty healthy Kunming mice were randomly divided into five groups:control group,nucleotides(NT)groups(4h,18h),nucleotides free(NF)groups(4h,18h).Control group and NF groups were fed with nucleotide-free diet.NT groups were fed with nucleotide-supplemented diet(0.25% nucleotides).On D 15,mice were lavaged with physiological saline(control)or LPS,and were killed 4 or 18 h later.Serum,liver,small intestine,and peritoneal macrophage were sampled in germfree state.Results:Hepatic Na+K+-ATPase,intestinal superoxide dismutase(SOD),serum total anti-oxidation ability,peritoneal macrophage-produced interleukin 10(IL-10)were increased,and intestinal malonaldehyde(MDA),serum alanine aminotransferase(ALT),intestinal myeloperoxidase(MPO),peritoneal macrophage-produced interleukin 1(IL-1)were decreased with nucleotides supplement.Conclusion:Exogenous nucleotides can help to maintain oxidation-antioxidation and inflammation-antiinflammation balance,and protect mice from injury under LPS immunostimulation.

Plasma D-dimer is one of the degradation products of the cross-linked fibrin hydrolyzed by fibrinolysin and is also a unique metabolite of secondary fibrolysis.The change of its content is a reliable indicator for the identification of the hypercoagulabale state in vivo and primary and secondary fibrinolysis,as well as for the observation of the effectiveness of thrombolytic therapy.In recent years,D-dimer determination has gained new clinical application.

The HPLC-ELSD method was used in the content determination of astragaloside, astragalosideⅠ, astragalosideⅡand astragalosideⅢ in Mongolia Radix Astragali (Astragalus membranaceus(Fisch.) Bge. var. mongholicus(Bge.) Hsiao) among 16 batches from various habitats. The DIKMA Diamonsil C18 (150 mm× 4.6 mm, 5μm) was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1.0 mL·min-1. And the column temperature was 30℃. The ELSD detector parameters were the drift tube temperature at 90℃, and the air flow rate of 2.8 L·min-1. The SPSS 16.0 software was used in the cluster analysis of content determination. The results showed that when the injection volume was within the range of 0.093 2-1.02μg (r = 0.999 5), 0.789-8.78μg (r = 0.999 7), 0.506-3.13μg (r = 0.999 6), and 0.016 1-1.38μg (r = 0.999 2) for astragaloside, astragalosideⅠ, astragalosideⅡ and astragalosideⅢ, respectively, the average recoveries were 97.55%, 98.61%, 99.68%, 98.58%with RSD of 1.2%, 1.3%, 1.3%, 1.2%, respectively. The results of cluster analysis showed that the single using of astragaloside as index was unable to differentiate Mongolia Radix Astragali from various habitats. However, the simultaneous determination of 4 types of astragalosides as indexes can differentiate Mongolia Radix Astragali from various habitats. It was concluded that the method was simple, quick and accurate, which can directly reflect the quality status of Mongolia Radix Astragali from different origins. It also provided new ideas for the quality control of Mongolia Radix Astragali.

Objective To investigate the correlation of serum interleukin-6 (IL-6) and C-reactive protien (CRP) lev?els with left atrial thrombus and severe spontaneous echocontrast (SEC) in patients with atrial fibrillation (AF). Methods A case-control study of patients with atrial fibrillation (n=76) was carried out. All patients were divided into control group (n=45) and study group (n=31) according to their conventional echocardiography performance. Serum IL-6 and CRP were exam?ined in both groups. Results The levels of serum IL-6 in patients with thrombus and severe SEC were (324.13±42.86) ng/L and (332.29±53.17) ng/L, respectivly which is higher than that in patient without thrombus or without severe SEC (108.75± 25.43) ng/L and (93.59 ± 27.82) ng/L respectively. In parallel, CRP levels in patients of thrombus and severe SEC were (66.97 ± 17.65) mg/L and (71.81 ± 20.19) mg/L respectively which is higher than that in patients without thrombus or without severe SEC (17.28±6.52) mg/L and (16.76±8.73) mg/L respectively. All differences were of statistically significant(P<0.05). Conclusion Increase of serum IL-6 and CRP as well as high systemic inflammatory state correlate with left atrial thombus and severe SEC in patients with AF.

AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8). The control group consumed an ordinary diet. The other two experimental groups were fed with a high fat diet,high fat plus 0.1% LA. After 6 weeks,the activities of digestive enzymes were examined. In order to evaluate the antioxidant status of the mice,superoxide dismutase (SOD),malondialdehyde (MDA),total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured. To investigate the molecular mechanisms underlying the effects of LA,the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system,accompanied by digestive and absorptive function impairment,was observed in HFD-fed mice. These changes were partially restored in the LA-treated group. DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes,digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD. The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.

Objective:To explore the effect of lipoic acid (LA) on chronic oxidative stress,cytokines and inflammatory gene expression with mice fed with high fat diet (HFD) and whether LA supplementation could prevent development of chronic inflammation.Methods:C57BL/6 mice were randomly assigned to three groups.The control group were administrated with an ordinary diet.The two experimental groups were fed with a high fat diet or high fat plus 0.1% LA.Antioxidants defense index such as SOD,CAT,GSH-Px and MDA were examined after 10 week.Cytokines such as IFN-?,IL-4,IL-6,TNF-? and IL-10 were examined after 10 week,respectively.Gene expression related to oxidative stress and inflammation were confirmed by QRT-PCR.Results:HFD led to potently weaken antioxidant defenses in mice.HFD significantly increased levels of IFN-?,IL-6 and TNF-?,and decreased levels of IL-4 and IL-10 in mice plasma.QRT-PCR results showed an up-regulation of inflammation related genes and a down-regulation antioxidant-related genes.Conclusion:LA is a possibly effective supplementation with HFD,both to prevent from the development of long-term oxidative stress and to attenuate chronic inflammation.

Objective To explore the influence of high fat diet on the intestinal gene expression profile in C57BL/6 mice. Method C57BL/6 mice were randomly assigned to two groups (n=8). The control group consumed an ordinary diet. The experimental group was fed with a high fat diet. All mice were sacrificed at the end of 6 w and the intestinal gene expressions were detected by oligonucleotide microarray analysis with Affymetrix GeneChip Mouse U430A consisting of 13 097 genes. Results Among the 13 097 genes obtained from gene expression profile analysis, there were 88 and 179 genes up -and down-regulated respectively, in mice fed with high fat diet compared with the control. The differentially expressed genes were mainly related to free radical oxidative stress, DNA repair, induction of apoptosis, transport, signal transduction and inflammation immune response. Conclusion High fat diet may widely modulate the expression of many genes in the intestine in mice.