OBJECTIVEThe study was to explore extracting and purifying technology of taxol from the branches of Taxus media.

METHODIn extracting phase, the solvent extraction, ultrasonic extraction and the supercritical CO2 fluid extraction were studied respectively; In purifying phase, the extracts were disposed by silica gel column chromatography and preparation lamella chromatography, then crystaled by N-Hexane. The content of taxol was detected by HPLC.

RESULTThe results showed that the recovery of extracting toxal with the method of ultrasonic was the highest and the selectivity of supercritical CO2 fluid extraction was the best; The sample after being extracted should be purified two stages by silica gel column chromatography with dichloromethane-chloroform-methane (53:44:3) as elution and the lamella chromatography was chloroform-ethyl acetate-methane (88:7:5) as elution, finally we reached the fawn crystal.

CONCLUSIONIn that production, the content of taxol reached 87. 3% and the recovery 89.7%, which indicat that the taxol is well enrichment.

Chloroform ; chemistry ; Chromatography, High Pressure Liquid ; Chromatography, Supercritical Fluid ; Methane ; chemistry ; Methylene Chloride ; chemistry ; Paclitaxel ; chemistry ; isolation & purification ; Taxus ; chemistry