High-intensity focused ultrasound (HIFU) is a newly-developed noninvasive technique recently. Being a safe, non-radioactive and reproducible therapy, high-intensity focused ultrasound has been extensively used in the clinical treatment for a variety of solid tumors such as uterine adenomyosis. This paper aims to make a comprehensive review about this technique, focusing on the mechanism, clinical indications and contraindications, safety, efficacy and the complications of HIFU for the treatment of uterine adenomyosis.

Objective To investigate the delivery efficacy of periadventitial delivery of 125I-iododeoxyuridine (125I-IUdR) in comparison of intravascular delivery to determine the optimal delivery method for inhibiting post-angioplasty restenosis. Methods In 8 pigs, one side carotid, subclavian and iliac arteries of each pig were injured by balloon angioplasty with a 20% overstretches. Then, 4 mCi of 125I-IUdR was delivered at each targeted vessel with periadventitial method in 4 pigs (periadventitial group) and with intravascular method via a porous balloon catheter in other 4 pigs (intravascular group). The animals survived for 5 hours and the blood radioactivity was investigated prior to and hourly after procedure until sacrifice. The targeted vessels and renal arteries (for control) were harvested for gamma-counting and histological observation. Meanwhile, the radioactivity in thyroid, liver, bladder, small bowel and each kidney also were measured to determine the biodistribution of 125I. The activities of 125I presented in arterial and tissue specimens were compared between the two delivery groups. The targeted arteries were histologically observed and the ratio of intima to media (I∶M ratio) was calculated. Results The target arterial walls in the periadventitial group had 3.4 times as much of 125I radioactivity as in the intravascular group, respectively (P=0.038); the blood activity in intravascular group was significantly higher than periadventitial group immediate after procedure (P<0.05) and intravascular delivery resulted in much higher activity in urine than periadventitial delivery (P<0.05). The systemic biodistributions of 125I-IUdR in the organs were slightly higher in the intravascular group (P>0.05). The mean I: M ratios in both groups were 0.05 without additional injury at the vessel wall. Conclusion The periadventitial delivery offered substantial advantage over intravascular approach with high local delivery efficacy. The apparent redistribution rate is more rapid following intravascular delivery.

Objective: To explore the effects of early higher protein nutrition support and growth hormone in patients with severe craniocerebral injury. Methods: 60 patients with severe craniocerebral injury were randomized into observation group and control group.The observation group (30 cases) had received on higher protein nutrition support [2-2.5 g/(kg?d)] and growth hormone for one week. The control group(30 cases) had received lower [1.2-1.5 g/(kg?d) protein nutrition support] for one week. The levels of protein index were determined. The clinical effect and complication were compared between the two groups. Results: The serum prealbumin, albumin, transferrin and hemoglobin in the observation group were significantly higher than those in the control group.The staying time in ICU was significantly shorter and the progrosis was better in the observation group. There were no statistical differences about the index of liver function, renal function and the morbility between the two groups. Conclusion: Early higher protein nutrition support and growth hormone can improve the nutrition status and the prognosis of patients with severe craniocerebral injury.

We have conducted a tireless practice and exploration in order to establish a solid,long-term and appropriate social practice basement for medical students,which is the platform for them carrying on social practice activities.Practice has proved that early preparation for basement construction is the basis,support from local government is the key,leaders' attention 、abundant fund and multi-sectional coordination are the guarantee,and that diverse forms as well as effective social practice activities is the fundamental way to promote basement construction.

Expression of epidermal growth factor receptor (EGFR) in SK BR 3 cells, a human breast carcinoma cell line,was analysed with immunohistochemistry. Inhibitory effects of TP40 on SK BR 3 cells growth and protein synthesis were analysed with crystal violet staining and 3 H leucine incorporation. Competitive assays were performed by the addition of excess of EGF. The results showed that the SK BR 3 cells exhibited large amounts of brown immunoperoxidase reaction indicative of EGFR. When the concentration of TP40 was in the range of 1 100?g/L,TP40 inhibited SK BR 3 cell growth and protein synthsis in a dose dependent form. An excess of EGF could completely block inhibitory effects of TP40. The results suggested that the human breast carcinoma SK BR 3 cells express EGFR at a high level. TP40 could significantly inhibit the growth of SK BR 3 cells. The cytotoxic effects of TP40 were specifically mediated by EGFR.

Objective To investigate CT examination in the evaluation of the percutaneous radio frequency ablation (RFA) in the treatment of hepatic carcinoma.Methods 50 cases of hepatic carcinoma (including primary hepatic cancer 36 and hepatic metastases 14) with 76 lesions treated by RFA associated with pre and post procedure data of CT follow up were analyzed retrospectively. CT examinations including plain and contrast enhanced CT scanning were carried out at 1 week and 3 weeks after the RFA, but sometimes with furthermore intermittent scanning at 3～6 months. Results Complete ablation of the tumors occurred in 49 lesions (64.5%) of 35 cases and incomplete ablation of tumors happened in 27 lesions (35.5%) of 18 cases. Among RFA treated 76 lesions, there were 9 with internal hemorrhage, 1 with abscess formation. 3 cases showed intraperitoneal bleeding; 6 cases demonstrated reactive pleural effusion and 1 case revealed with small amount of pneumothorx.Conclusions The contrast enhanced CT scanning provides a great diagnostic value for evaluation of the efficacy of hepatic tumor treated by RFA and also the local recurrence and complications.

Objective To compare the effectiveness and advantage of salvage liver transplantation post-resection with primary liver transplantation for HCC patients. Methods Medline, Embase, Cochrane, CBMdisc, CNKI and Wanfang Database were searched. Randomized and nonrandomized controlled trials comparing primary liver transplantation with salvage liver transplantation were analyzed. Ten retrospective studies involving 1115 patients were included. 164 received salvage liver transplantation,and 951 patients received primary liver transplantation. Results In most researches, the two groups of patients were similar for age, sex,and cirrhosis severity at time of transplantation. Some researches showed no difference in tumor size and number between the two groups. Few studies referred to microvascular invasion. Most of the researches showed there was no significant difference in blood loss,operative time,overall survival and disease-free survival between the two groups. Conclusion Compared to primary liver transplantation, salvage liver transplantation after liver resection is associated with similar operative time, risks and overall survival. Resection first and salvage liver transplantation for recurrent tumors or liver failure seem to be a feasible treatment strategy for hepatocelluar carcinoma,especially in early-stage.

Objective To evaluate preventive and therapeutic effects of low dose of unfractioned heparin on severe sepsis. Methods 52 Patients with severe sepsis hospitalized in Intensive Care Units were randomly divided into control group and heparin group. On the basis of conventional therapy, patients with sepsis were given low dose of unfractioned heparin or isovolume physiologic saline by continuous intravenous drip infusion over 5 days. Acute Physiologic and Chronic Healthy Evaluation (APACHE-Ⅱ) score, blood platelet count(BPC), prothrombin time(PT), activated partial thromboplastin time(aPTT), thrombin time(TT), plasma fibrinogen(FDP) and D-dimer levels were assessed on admission, 3rd, 5th day after intervention. Incidence of disseminated intravascular coagulation(DIC) and 28-day mortality between heparin group and control group were compared. Results There were no significant difference in APACHE-Ⅱ, BPC, PT, aPTT, TT, and FDP levels, incidence of DIC, and 28-day mortality between heparin group and control group. D-dimer levels in both groups decreased along with time. D-dimer levels on 5th day after treatment in heparin group was markedly decreased than that of control group(740?135?g/L vs 853?179?g/L, P

Objective: To explore the differential expression of tumor associated antigen CA X in tumor cells in vitro and in vivo . Methods: Tumor xenografts of the human lung adenocarcinoma cell line SpcA1 were grown in athymia mice. The antigen was measured before and after xenograft by Western blotting and flow cytometric analysis. Results: The antigen CA X was a glycoprotein with a molecular weight of 170 000 to 200 000 . Human lung adenocarcinoma cell lines regain expression of antigen CA X when xenografted into athymia mice. Conclusion: These data indicate that CA X synthesis in human lung adenocarcinoma cells SpcA1 is regulated by factors presented only in the xenograft.

Objective To study the effects of lipopolysaccharide(LPS) on the expression of toll like receptor 4 (TLR4), reactive oxygen species(ROS) and on the proliferation of cells as well as secretion of six proinflammmatory cytokines including TNF-α, IL-1, IL-6, IL-8, IL-10, IL-12 levels in SKOV3 cells. And to explore the mechanism of SKOV3 cells in regulation. Methods Cultured primary SKOV3 cells were stimulated with different concentrations of LPS (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 20 μg/ml) for 4 h, the TLR4 expression in SKOV3 cells were examined by flow cytometry;1 μg/ml LPS stimulated SKOV3 for 4 h, 8 h, 12 h, 24 h respectively, the TLR4 expression and cell cycle in SKOV3, cell proliferation, ROS level as well as cells and TNF-α and IL-1, IL-6, IL-8, IL-10, IL-12 levels in the culture medium were assayed by flow cytometry, MTT, CBA assay respectively. Results LPS with different concentrations of LPS stimulation in-duced an increased TLR4 expression, however, the expression was reduced when LPS concentration up to 10 μg/ml. LPS stimulation for 4 h, 8 h induced an increased TLR4 expression and cell proliferation. Stimulated for 24 h, however, the TLR4 expression and cell growth were inhibited in S period. Meanwhile, LPS stimulation for 4 h, 8 h, 12 h, 24 h induced a higher ROS secretion in comparison with control group. LPS stimulation induced a stronger cytokine response in comparison with control group, as demonstrated by the production of TNF-α, IL-1, IL-6, IL-8 secretion in cultured SKOV3 cells, while IL-10 and IL-12 with low expression have no obvious difference in the all medium samples. Conclusion TLR4 expression, cell proliferation, ROS and proin-flammmatory cytokine secretion could be induced in SKOV3 through LPS stimulation. The study provide new ex-periment evidences for human ovarian cells SKOV3 immunity regulation and inflammation reaction to promote cells inhibition after LPS stimulation.