1.Study on the Mechanism of Preventing and Treating Scar by Traditional Chinese Medicine
Journal of Zhejiang Chinese Medical University 2013;(10):1265-1267
[Objective]The purpose of the review was to summarize the research progress on traditional Chinese medicine to prevent and treat scar and its mechanism in recent 10 years. [Methods]Retrieving the literature of the past 10 years on Chinese medicine to prevent scar , it is to summarize scar mecha-nism of prevention and cure both in vivo and in vitro experiments. [Result]Main mechanism of preventing and treating scar by traditional Chinese medicine is through studying fibroblasts and col agen. The specific mechanism is that Chinese medicine can inhibit the growth of scar fibroblasts in a con-centration-time-dependent manner, so as to reduce the scar tissue and control of infection and other complications; Additionly, by adjusting the levels of col agenⅠ,Ⅲ, Chinese medicine can regulate synthesis of col agen, reduce scar, and improve the quality of wound repair. [Conclusion]Traditional Chinese medicine has certain advantages in preventing and treating scar, but needs to further standardize methodology to broaden ideas. So Chinese medicine has a promising furure on anti-scar in the clinic.
2.The inhibitory effect of ghrelin on insulin release of mouse islet ?-cell line and its mechanism
Lin LI ; Yonghua WU ; Tianpei HONG
Chinese Journal of Diabetes 2000;0(06):-
Objective To explore the effect of ghrelin on insulin release of mouse pancreatic islet ?-cell line(NIT-1 cells) and its probable mechanism.Methods NIT-1 cells were incubated in high-glucose DMEM with ghrelin.Then,the media was sampled for the assay of insulin by RIA.The(mRNA) expressions of glucose transporter 2(GluT2),pancreatic-duodenal homeobox-1(PDX-1),inwardly rectifying potassium channel with two transmembrane regions(Kir6.2) and sulphonylurea receptor 1(SUR-1) in the cells were detected by using RT-PCR.The cell proliferation was determined by MTT assay.Results(1) 10~(-9)mol/L to 10~(-7)mol/L of ghrelin inhibited dose-dependently the high-glucose challenged insulin release of the NIT-1 cells.(2) The mRNA expression of Kir6.2,but not GluT2,PDX-1and SUR-1,was down-regulated by 10~(-7)mol/L of ghrelin.(3) Ghrelin had no effect on proliferation of the cells.Conclusions Ghrelin inhibits high-glucose induced insulin secretion of the islet ?-cells.This effect may be secondary to the down-regulation for the expression of Kir6.2,(a component) of ATP-sensitive potassium channel.
3.Analysis on Chinese literature for MRI major subsystem eliminable faults
Xinhuai WU ; Yonghua ZHAO ; Zhouzhu HAN
Chinese Medical Equipment Journal 1989;0(01):-
MRI system is composed of many independent subsystems, in which RF, Gradient, Coil, MRI magnet subsystem are the most important and complex subsystems. Every subsystem consists of different components. Based on the analysis of MRI eliminable faults in CBMDisc (1995 ~ 2006), the types and occurrence frequency of MRI eliminable faults are expatiated so as to provide reliable data for reference.
4.Analysis on Army Writers of Chinese literature on MRI System Repairing and Maintenance from 1994 to 2007
Xinhuai WU ; Yonghua ZHAO ; Zhouzhu HAN
Chinese Medical Equipment Journal 2004;0(09):-
Based on the analysis on "China biomedical medical literature database"(CBMdisc) in the field of MRI Maintenance composed by army writers from May,1994 to 2007,the following results can be obtained: the total number of writers amounts to 75,106 person-time,27 of them are the first writers,and 11 are main writers;among the total 48 pieces of work,33 pieces are written by more then one writer;the unit with the largest number of published writers is Jinan Military Area Command with Nanjing Military Area Command the second,Lanzhou Military Area Command the third;one main writer has a maximum of 7 pieces.The research makes a close study of the army writers in field of MRI maintenance,indicating the lack of intellectual personnel in the domain of Medicine Engineering in military system.
5.Computer-Administered Neurobehavioral Evaluation System and Its Application in Evaluation of Children's Neurobehavioral Functions
Hailei WU ; Jinliang ZHANG ; Yonghua HU
Journal of Environment and Health 1993;0(03):-
Computer-administered neurobehavioral evaluation system (NES)was developed based on the neurobehavioral core test battery (NCTB)recommended by WHO/NIOSH in 1985. Compared with NCTB,the reliability and validity of NES was much improved and some disadvantages of NCTB were made up in NES. This article reviewed the history of NES development,its application in the evaluation of children's neurobehavioral functions and its defects. The studies suggested that some items of NES test,such as finger tapping,continuous performance test and hand-eye coordination,could be used to evaluate earlier damage induced by chemicals on neurobehavioral functions of children.
6.Analysis on MRI system eliminable faults in Chinese literature
Xinhuai WU ; Yonghua ZHAO ; Zhouzhu HAN
Chinese Medical Equipment Journal 2003;0(10):-
MRI system,as an imaging diagnosis equipment,plays a very important role in diagnosis and treatment.For the steady avail of MRI system,MRI eliminable faults in CBMDisc(1995~2006) were analyzed.The types and occurrence frequency of MRI eliminable faults were elaborated so as to provide reliable data for reference.
7.Expression of THANK in human PBMC activated with different stimulators
Dong WU ; Feng SHEN ; Yonghua LOU ; Binghua JIAO ; Mengchao WU
Journal of Cellular and Molecular Immunology 2001;17(4):304-306
Aim To analyze THANK gene expression in peripheral blood mononuclear cells(PBMC) stimulated with different stimulators and to clone whole length human THANK gene. Methods PBMC were conventionally isolated and cultured in RPMI1640 containing 10% FCS. After stimulated with LPS,TNF α ,IL 2,IFN γ ,PHA or PMA,the THANK gene expression in PBMCs was analyzed by RT PCR and THANK cDNA was cloned. Result RT PCR detection showed that THNAK gene expressed in PBMCs after stimulated with interferon γ for 3 days, whereas THANK'expression could not be detected after stimulated with LPS,TNF α ,IL 2,IFN γ ,PHA or PMA respectively. Then THANK gene was cloned by cloning PCR product and sequenced. Conclusion Human THANK gene is cloned successfully, thus providing the possibility for further research of THANK'function.
8.Study and experience from work of computer X-ray photography
Yuefeng HAN ; Xinhuai WU ; Zhouzhu HAN ; Xiangyong GAO ; Yonghua ZHAO
Chinese Medical Equipment Journal 1993;0(06):-
Technology of radiotheraphy has stepped into an era of digital photography and replaced the screen piece combination system.This thesis briefly expounds the advantages and prospect of computer X-ray photography,and discusses its problems and effective solutions in clinical practice.
9.Study of the Correction between the Age Related Hearing Loss and the Cytoactivity Factors of the Cochlear Hair Cell in C57BL/6J Mice
Liangqiang ZHOU ; Shaoling WU ; Yan WANG ; Hanqi CHU ; Yonghua CUI
Journal of Audiology and Speech Pathology 2009;17(4):363-368
Objective To establish the mice model of AHL, to investigate the relationship between AHL and the cytoactive factors of the cochlear hair cells in C57BL/6J mice, and to classify the presbycusis models of the C57BL/6J mice. Methods C57BL/6J mice were divided into 6 experimental groups by age (A: 3 months old(m), B: 8 m, C: 9 m, D: 10 m, F: 17 m, G: 18 m) . The auditory functions mice were measured by auditory brainstem response (ABR) with the stimulus click and toneburst at 6 kHz and 8 kHz. 3 months later, Groups C , G, E and H were tested again for ABR. After ABR testing, the cytoactive of the hair cells was detected by succinate dehydrogenase staining and surface preparation technique(two mice from each group except groups C and G). Results The ABR thresholds elevated with age, and the marked change of the cochlea was the degeneration of the cytoactive of the cochlear hair cells, especially those of the outer hair cells. In the beginning, the basement of the basal membrane suffered from the mitochondrion degeneration in the outer hair cells, then it spread to the top region. Subsequently, the inner hair cells were involved. Conclusion C57BL/6J mouse was a typical animal model for the AHL,and the main change of the cochlea was the degeneration of the hair cells, especially the outer hair cells. Thus, C57BL/6J mice can be used as a suitable animal model for the study of presbycusis.
10.Fault Statistics and Maintenance Cost Analysis About Using Digital Gastrointestinal Machine of SIREGRAPH CF
Xinhuai WU ; Zhouzhu HAN ; Yonghua ZHAO ; Jinpu LI
Chinese Medical Equipment Journal 1989;0(02):-
Statistically analyses SIEMENS SIREGRAPH CF digital gastrointestinal machine the breakdown and maintenance cost which appears in the radialized practice by the note for the fault maintenance,making fault statistics by Excel,discusses in the emission practice,how displays the equipment benefit fully and saves the service cost possibly,and the results of digital gastrointetinal machine's fault frequency of system is got.to discuss the reasonality of this kind of equipment voluntarily services in second level of hospital.[Chinese Medical Equipment Journal,2008,29(2):78-79]