Objectives To investigate the regularity of fluctuation and clinical senses of serum c reactive protein (CRP) level in patients with acute cerebral infarction (ACI) Methods 100 subjects (56 males and 44 females) were divided into five groups including 24 large artery arteriosclerosis (LAA), 27 small artery occlusion (SAO), 2 cardiac embolism(CE), 34 other Certain(OC) and 13 undetermined etiology according to Trial of ORG 10172 in Acute Stroke Treatment (TOAST) Through enzyme linked immunoabsorbent assay (ELISA), we detected and analyzed CRP levels of 100 inpatients Results The risk factors of stroke included coronary heart disease , hypertension, diabetes and intoxication of CO and so on Among the above mentioned, the level of CRP and rate of positive CRP of patients who poisoned by CO was the highest That of coronary heart disease group was lower However, there was no notable difference between hypertension and diabetes groups Conclusions CRP may play a certain role in the occurrence and development of ACI It represents different levels in different types of stroke

Objective To study the regulatory effect of ethanol extract of glossy privet fruit and its monomer tyrosol on the adhesion and migration of human epidermal melanocytes.Methods Epidermal melanocytes were isolated from human foreskin,and subjected to a primary culture.Mter 3-5 passages,the melanocytes were treated with various concentrations of ethanol extract of glossy privet fruit (0.0375-0.6 mg/ml)and tyrosol (0.125-2 mmol/L) for 24-72 hours.The XTT colorimetric assay was carried out to evaluate the proliferation of melanocytes,fibronectin (FN)-coated culture plates were used to evaluate the adhesion activity of melanocytes,and Transwell assay was conducted to assess the migration activity of melanocytes.Confocal laser microscopy was utilized to observe the structure and distribution of actin cytoskeleton in melanocytes,and cellular fluorescence intensity was determined by a semi-quantitative analysis.Statistical analysis was carried out by using unpaired t test.Results The adhesion activity of melanocytes to FN was significantly enhanced by the ethanol extract of 0.0375-0.6 mg/ml from glossy privet fruit (P ＜ 0.05 or 0.01),and by tyrosol of 0.5-2 mmol/L (P ＜ 0.05 or 0.01).As XTT assay showed,neither the ethanol extract of 0.15 mg/ml nor tyrosol of 2 mmol/L had cytotoxicity or promotive effect on cell proliferation.Hence,0.15 mg/ml and 2 mmol/L were determined as the working concentrations of ethanol extract and tyrosol respectively.The number of cells migrating through micropore membranes per high-power field (× 200) was 43.7 and 51.0 in melanocytes treated with the ethanol extract of 0.15 mg/ml and tyrosol of 2 mmol/L,respectively,significantly higher than that in untreated melanocytes (20.3,both P ＜ 0.01).Compared with untreated melanocytes,those treated with the ethanol extract of 0.15 mg/ml and those with tyrosol of 2 mmol/L showed higher intracellular fluorescence intensity (P ＜ 0.01) and more stress fiber bundles which congregated inside the cell membrane and around the nuclei.Conclusions The ethanol extract of glossy privet fruit and its monomer tyrosol can promote the adhesion and migration of human melanocytes in vitro,likely by promoting the congregation of actin cytoskeleton in melanocytes.

To investigate the correlation between p16 gene mutation and laryngeal cancer biological behavior as well as its prognosis in laryngeal cancer.Method:24 speciments of primary laryngeal cancer and 10 speciments with benign lesion in larynx were examined for mutations in exon2 of p16 by using PCR-SSCP silver stainning technique.Result:Mutations frequency of laryngeal cancer was62.5% (15/24).Nothing was found in 10 cases with laryngeal benign lesion.Conclusion:There is a strong correlation between p16 gene mutation and the biological behavior of chinese laryngeal cancer, such as histologic differentiation, invasion stage, and regional lymph nodes metastasis(P≤0.05).PCR-SSCP silver tainning technique is one of the most sensitive and simplest measure for detecting genetic mutation.It is worth using in clinical laboratory because of its readiness, repetition and lower cost.

Objective We conducted a study using MRI and ambulatory blood pressure monitoring(ABPM) to determine whether an inapporpriately low nocturnal blood pressure,or an excess fall in nocturnal blood pressure,might be responsible for lacunar infarct.Method ABPM and Casul blood pressure(CBP) were examined in 35 hypertentives with lacunar infarct( LI） and 33 hypertentives without lacunar infarct as control group.Results There is no significant difference of CBP between two groups.But the mean nighttime systolic blood pressure(nSBP) and diastolic blood pressure(nDBP) in patients with lacunar infarct were significantly smaller than in patients without lacunar infarct.The ratio of nSBP/dSBP and nDBP/dDBP in SI were smaller than in control group respectively.Conclusions The results indicate that an inappropriately low nocturnal blood pressure,or an excessive fall in nocturnal blood pressure,is associated with lacunar infarct.It is necessary not only to controlhigh blood pressure but also to pay attention to circadian changes of blood pressure during the course of anti-hypertensive treatment.

BACKGROUND: At present, international classification of diseases by WHO, cerebrovascular disease classification used in China and TOAST classification used abroad are all based on patho-physiology. There is no report about human cerebral infarction by magnetic imaging types in Beijing and other areas.OBJECTIVE: To analyze retrospectively the subtype distribution of acute cerebral infarction of patients in Beijing by magnetic resonance imaging in order to make Beijing patients with acute ischemic stroke receive more exact and individualized treatment.DESIGN: Retrospective case study.SETTING: Department of Neurology, General Hospital of Beijing Military Area Command of Chinese PLA.PARTICIPANTS: The study was carried out in General Hospital of Beijing Military Area Command of Chinese PLA, Beijing Sino-Japan Friendship Hospital, Beijing Chaoyang Hospital, Beijing Naval General Hospital and Beijing Jishuitan Hospital between January and December 2004. Totally 602 inpatients with ischemic stroke who received treatment in each hospital were recruited. They were 352 male and 250 female with average age of (65.6±10.2) years.METHODS: The 602 patients with acute ischemic cerebral stroke who received the treatment in the 5 hospitals from Dongcheng, Xicheng,Chaoyang and Haidian districts in Beijing were recruited in the study. According to results of magnetic resonance imaging of brain, the maximal infarction area were determined. The patients were classified as: large infarction: over 1 lobe of brain, ＞ 5.0 cm in diameter; moderate infarction: less than 1 lobe of brain, 3.1-5.0 cm; small infarction: 1.6-3.0 cm; lacunar infarction, ＜ 1.5 cm.MAIN OUTCOME MEASURES: Constitution ratio of MRI classification of patients in each groupRESULTS: Totally 602 patients with ischemic stroke participanted in final result analysis. Among the patients with acute cerebral infarction of 5 hospitals in Beijing, 280 (46.5%) suffered from lacunar infarction, 299(38.1%) from small infarction, 57 (9.4%) from moderate infarction, and 36 (6%) from large infarction. There were significant differences between lacunar infarction and other types (P ＜ 0.01).CONCLUSION: Acute cerebral infarction of patients of Beijing mostly occurs in the type of lacunar infarction. It suggests that small arterial diseases are the major disease type of ischemic stroke in Beijing. This MRI classfication can be used for early classification, treatment guidance, evaluation and prognosis of acute ischemic cerebral stroke.

Objective To study the effects of lipopolysaccharide(LPS) on the expression of toll like receptor 4 (TLR4), reactive oxygen species(ROS) and on the proliferation of cells as well as secretion of six proinflammmatory cytokines including TNF-α, IL-1, IL-6, IL-8, IL-10, IL-12 levels in SKOV3 cells. And to explore the mechanism of SKOV3 cells in regulation. Methods Cultured primary SKOV3 cells were stimulated with different concentrations of LPS (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 20 μg/ml) for 4 h, the TLR4 expression in SKOV3 cells were examined by flow cytometry;1 μg/ml LPS stimulated SKOV3 for 4 h, 8 h, 12 h, 24 h respectively, the TLR4 expression and cell cycle in SKOV3, cell proliferation, ROS level as well as cells and TNF-α and IL-1, IL-6, IL-8, IL-10, IL-12 levels in the culture medium were assayed by flow cytometry, MTT, CBA assay respectively. Results LPS with different concentrations of LPS stimulation in-duced an increased TLR4 expression, however, the expression was reduced when LPS concentration up to 10 μg/ml. LPS stimulation for 4 h, 8 h induced an increased TLR4 expression and cell proliferation. Stimulated for 24 h, however, the TLR4 expression and cell growth were inhibited in S period. Meanwhile, LPS stimulation for 4 h, 8 h, 12 h, 24 h induced a higher ROS secretion in comparison with control group. LPS stimulation induced a stronger cytokine response in comparison with control group, as demonstrated by the production of TNF-α, IL-1, IL-6, IL-8 secretion in cultured SKOV3 cells, while IL-10 and IL-12 with low expression have no obvious difference in the all medium samples. Conclusion TLR4 expression, cell proliferation, ROS and proin-flammmatory cytokine secretion could be induced in SKOV3 through LPS stimulation. The study provide new ex-periment evidences for human ovarian cells SKOV3 immunity regulation and inflammation reaction to promote cells inhibition after LPS stimulation.

Objective:To explore the e ects of clinical treatment and protection on endothelial function of early essential hypertension patients treated with YiNianJiangYa(YNJY) soup in southern Guangdong area.Methods:79 early essential hypertension cases with traditional Chinese medicine(TCM) syndrome of phlegm stasis blocking collaterals,excess of liver yang were randomly divided into treatment and control groups,treated with YNJY soup and TianMaGouTeng(TMGT) soup respectively for two courses of treatment.Di erences of clinical treatment e ect,blood pressure and pulse pressure after treatment,as well as plasma endothelin-1(ET-1) and von Willebrand factor(vWF) between the two groups were measured.Results:The clinical total e ective rate of treatment group was higher than that of control group(P 0.05).Conclusion:YNJY soup can signi cantly improve clinical treatment e ect of the early essential hypertension patients,has good e ect of decreasing blood pressure and no obvious side e ects.It has many e ects that reduces PP and the levels of plasma ET-1,vWF,may be related to protection of endothelial function,thereby improve the artery exibility.

Objective To investigate the medical cost of 70 patients with panic attack and efficacy of selective serotonin reuptake inhibitors (SSRIs) in the treatment Methods The course, medical cost, types and times of main examination of all 70 patients were reviewed and analyzed The effect of SSRIs on the patients was studied with Anxiety and Depression Scale Results The 70 patients had long course, high medical cost and received many unnecessary medical examinations SSRIs were effective on patients with panic attack in light of Anxiety and Depression Scale Conclusion Patients with panic attack have long course and spend much unnecessary cost before final diagnosis SSRIs is useful and safe in the treatment of panic attack

Objective To investigate the relationship between the protein kinase A (PKA) and apoptosis of cultured neurons after hypoxia Methods Model of cultured rat neuron under hypoxia condition was established. Rp cAMP, a specific inhibitor for PKC, at 4 different concentrations were separately cocultured for 2 h with the neurons having been cultivated under hypoxic condition for different times The activity of PKA, the expression of caspase 3 and the situation of neuron apoptosis were studied Results With the prolonging of hypoxic time the activity of PKA was increased significantly And the expression of activated caspase 3 and apoptotic DNA were increased as well The positive rate of fluorescence staining and the average fluorescent intensity of caspase 3 and TUNEL were significantly decreased along with the increasing concentration of Rp cAMP Conclusion ① PKA and caspase 3 are involved in the neuronal apoptosis after hypoxia ② Rp cAMP can attenuate the hypoxic neuronal apoptosis through the signal transduction of caspase 3 ③ The activating of PKA can aggravate hypoxic neuron apoptosis

Objective To explore the effect and possible mechanism of protein kinase C (PKC) on the apoptosis of cultured neurons after hypoxia Methods Model of cultured rat neurons under hypoxia condition was established. Calphostin C, an inhibitor for the catalytic subunit of PKC, at 4 different concentrations were separately cocultured for 2 h with the neurons having been cultivated under hypoxic condition for different times The activity of membrane PKC (mPKC), the expression of Bcl 2 and the situation of neuron apoptosis were studied Results With the prolonging of hypoxic time the activity of mPKC was increased significantly And the expression of Bcl 2 was decreased obviously and positive rate of TUNEL were significantly increased in a calphostin C concentration dependent manner Conclusion ① The activation of mPKC and Bcl 2 are involved in the apoptosis of neurons after hypoxia ② Hypoxia and calphostin C can aggravate the hypoxic neuronal apoptosis through the signal transduction of Bcl 2 ③ The activation of PKC can protect neuron against hypoxia