Objectives To investigate the regularity of fluctuation and clinical senses of serum c reactive protein (CRP) level in patients with acute cerebral infarction (ACI) Methods 100 subjects (56 males and 44 females) were divided into five groups including 24 large artery arteriosclerosis (LAA), 27 small artery occlusion (SAO), 2 cardiac embolism(CE), 34 other Certain(OC) and 13 undetermined etiology according to Trial of ORG 10172 in Acute Stroke Treatment (TOAST) Through enzyme linked immunoabsorbent assay (ELISA), we detected and analyzed CRP levels of 100 inpatients Results The risk factors of stroke included coronary heart disease , hypertension, diabetes and intoxication of CO and so on Among the above mentioned, the level of CRP and rate of positive CRP of patients who poisoned by CO was the highest That of coronary heart disease group was lower However, there was no notable difference between hypertension and diabetes groups Conclusions CRP may play a certain role in the occurrence and development of ACI It represents different levels in different types of stroke

BACKGROUND: At present, international classification of diseases by WHO, cerebrovascular disease classification used in China and TOAST classification used abroad are all based on patho-physiology. There is no report about human cerebral infarction by magnetic imaging types in Beijing and other areas.OBJECTIVE: To analyze retrospectively the subtype distribution of acute cerebral infarction of patients in Beijing by magnetic resonance imaging in order to make Beijing patients with acute ischemic stroke receive more exact and individualized treatment.DESIGN: Retrospective case study.SETTING: Department of Neurology, General Hospital of Beijing Military Area Command of Chinese PLA.PARTICIPANTS: The study was carried out in General Hospital of Beijing Military Area Command of Chinese PLA, Beijing Sino-Japan Friendship Hospital, Beijing Chaoyang Hospital, Beijing Naval General Hospital and Beijing Jishuitan Hospital between January and December 2004. Totally 602 inpatients with ischemic stroke who received treatment in each hospital were recruited. They were 352 male and 250 female with average age of (65.6±10.2) years.METHODS: The 602 patients with acute ischemic cerebral stroke who received the treatment in the 5 hospitals from Dongcheng, Xicheng,Chaoyang and Haidian districts in Beijing were recruited in the study. According to results of magnetic resonance imaging of brain, the maximal infarction area were determined. The patients were classified as: large infarction: over 1 lobe of brain, ＞ 5.0 cm in diameter; moderate infarction: less than 1 lobe of brain, 3.1-5.0 cm; small infarction: 1.6-3.0 cm; lacunar infarction, ＜ 1.5 cm.MAIN OUTCOME MEASURES: Constitution ratio of MRI classification of patients in each groupRESULTS: Totally 602 patients with ischemic stroke participanted in final result analysis. Among the patients with acute cerebral infarction of 5 hospitals in Beijing, 280 (46.5%) suffered from lacunar infarction, 299(38.1%) from small infarction, 57 (9.4%) from moderate infarction, and 36 (6%) from large infarction. There were significant differences between lacunar infarction and other types (P ＜ 0.01).CONCLUSION: Acute cerebral infarction of patients of Beijing mostly occurs in the type of lacunar infarction. It suggests that small arterial diseases are the major disease type of ischemic stroke in Beijing. This MRI classfication can be used for early classification, treatment guidance, evaluation and prognosis of acute ischemic cerebral stroke.

Objective We conducted a study using MRI and ambulatory blood pressure monitoring(ABPM) to determine whether an inapporpriately low nocturnal blood pressure,or an excess fall in nocturnal blood pressure,might be responsible for lacunar infarct.Method ABPM and Casul blood pressure(CBP) were examined in 35 hypertentives with lacunar infarct( LI） and 33 hypertentives without lacunar infarct as control group.Results There is no significant difference of CBP between two groups.But the mean nighttime systolic blood pressure(nSBP) and diastolic blood pressure(nDBP) in patients with lacunar infarct were significantly smaller than in patients without lacunar infarct.The ratio of nSBP/dSBP and nDBP/dDBP in SI were smaller than in control group respectively.Conclusions The results indicate that an inappropriately low nocturnal blood pressure,or an excessive fall in nocturnal blood pressure,is associated with lacunar infarct.It is necessary not only to controlhigh blood pressure but also to pay attention to circadian changes of blood pressure during the course of anti-hypertensive treatment.

Objective To discuss the methods of specification and quantification of thinking process of syndrome differentiation and treatment of old and famous TCM practitioners; To inherit the clinical experience and ideas of TCM practitioners more effectively. Methods Based on one typical case of Professor Lv Renhe treating chronic nephritis, indexing the prescriptions with TCM Subject Headings thesaurus and analyzing them with “Chinese medicine prescription intelligence analysis system” (“CPIAS” for short), relative dose, comprehensive property, comprehensive taste, comprehensive channel tropism, and prescription efficacy were under quantitative analysis. Results The typical case included two prescriptions of initial diagnosis and six diagnosis. CPIAS quantitative analysis showed that relative dose, comprehensive property, comprehensive taste, comprehensive channel tropism, and prescription efficacy were in accordance with syndrome differentiation and treatment of Professor Lv Renhe for chronic nephritis. Conclusion This method has guiding significance for studying and inheriting the speculative regularities and academic thoughts of Professor Lv Renhe.

Objective To know the relationship between the uterine fibroid symptom severity and the quality of life andthe correlation among all the subscales of the quality of life base through analysis of the questionnaires of the uterine fibroids health-related quality of life.Methods Retrospectively analyze the UFS-QOL subscales and SF-36 subscales of the 302 women with uterine fibroids who underwent ultrasound ablation in the high intensity focused ultrasound center of the First Affiliated Hospital of Chongqing Medical University in 2013.Results The Cronbach'sa of the UFS-QOL symptom severity subscale and the HRQL subscales were 0.794 and 0.953,reflecting adequate internal consistency.Except the sexual function field,the symptomatic group reported significantly greater symptom severity and worse health-related quality of life(HRQL) than the asymptomatic group(P＜ 0.05).Spearman's correlations between the symptom severity subscale and each HRQL subscales were moderately in the asymptomatic group,there was a linear positive correlation between the UFS-QOL subscales of activities,energy/mood and control(P＜ 0.05).In the symptomatic group,there was a linear negative correlation between the symptom severity subscale and the UFS-QOL subscales of concern,activities,energy/mood(P＜0.05),and there was a linear positive correlation between the UFS-QOL subscale of activities and the UFS-QOL subscales of concern,energy/mood.Conclusion Uterine fibroids related symptoms have an impact on the quality of life in women with uterine fibroids,and the assessments among the subscales of the quality of life influence each other.

In order to investigate the interelationship between copper deficiency and lipidemia, an experiment was done in weanling rats fed normol and copper deficient diet for one month. At the end of experimental period, the growth rate, the contents of copper, zinc, cholesterol and triglyceride in plasma and the activity of monoamine oxidase (MAO) in plasma were measured. The differences between the two diets were compared by student's test and the relation between plasma copper and plasma cholesterol or MAO was ascertained by regression analysis.The results showed that a lower body weight gain and a highly significant hypercholesterolemia were found in copper deficiency rats and the plasma cholesterol concentration correlated reversely with the plasma copper (r = -0.724, p

Objective:To explore the e ects of clinical treatment and protection on endothelial function of early essential hypertension patients treated with YiNianJiangYa(YNJY) soup in southern Guangdong area.Methods:79 early essential hypertension cases with traditional Chinese medicine(TCM) syndrome of phlegm stasis blocking collaterals,excess of liver yang were randomly divided into treatment and control groups,treated with YNJY soup and TianMaGouTeng(TMGT) soup respectively for two courses of treatment.Di erences of clinical treatment e ect,blood pressure and pulse pressure after treatment,as well as plasma endothelin-1(ET-1) and von Willebrand factor(vWF) between the two groups were measured.Results:The clinical total e ective rate of treatment group was higher than that of control group(P 0.05).Conclusion:YNJY soup can signi cantly improve clinical treatment e ect of the early essential hypertension patients,has good e ect of decreasing blood pressure and no obvious side e ects.It has many e ects that reduces PP and the levels of plasma ET-1,vWF,may be related to protection of endothelial function,thereby improve the artery exibility.

Objective To study the effects of lipopolysaccharide(LPS) on the expression of toll like receptor 4 (TLR4), reactive oxygen species(ROS) and on the proliferation of cells as well as secretion of six proinflammmatory cytokines including TNF-α, IL-1, IL-6, IL-8, IL-10, IL-12 levels in SKOV3 cells. And to explore the mechanism of SKOV3 cells in regulation. Methods Cultured primary SKOV3 cells were stimulated with different concentrations of LPS (0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 20 μg/ml) for 4 h, the TLR4 expression in SKOV3 cells were examined by flow cytometry;1 μg/ml LPS stimulated SKOV3 for 4 h, 8 h, 12 h, 24 h respectively, the TLR4 expression and cell cycle in SKOV3, cell proliferation, ROS level as well as cells and TNF-α and IL-1, IL-6, IL-8, IL-10, IL-12 levels in the culture medium were assayed by flow cytometry, MTT, CBA assay respectively. Results LPS with different concentrations of LPS stimulation in-duced an increased TLR4 expression, however, the expression was reduced when LPS concentration up to 10 μg/ml. LPS stimulation for 4 h, 8 h induced an increased TLR4 expression and cell proliferation. Stimulated for 24 h, however, the TLR4 expression and cell growth were inhibited in S period. Meanwhile, LPS stimulation for 4 h, 8 h, 12 h, 24 h induced a higher ROS secretion in comparison with control group. LPS stimulation induced a stronger cytokine response in comparison with control group, as demonstrated by the production of TNF-α, IL-1, IL-6, IL-8 secretion in cultured SKOV3 cells, while IL-10 and IL-12 with low expression have no obvious difference in the all medium samples. Conclusion TLR4 expression, cell proliferation, ROS and proin-flammmatory cytokine secretion could be induced in SKOV3 through LPS stimulation. The study provide new ex-periment evidences for human ovarian cells SKOV3 immunity regulation and inflammation reaction to promote cells inhibition after LPS stimulation.

Objective To evaluate the role of ERK1/2 signal transduction pathway in sevoflurane postconditioning-induced reduction of oxygen-glucose deprivation(OGD)injury in rat hippocampal slices.Methods Male adult SD rats weighing 80-100 g were anesthetized with ether and decapitated.The hippocampi were removed and sagittally sliced(400μm thick)and placed in artificial cerebrospinal fluid(aCSF)aerated with 95％ O2-5％CO2.Fifty hippoeampal slices were randomly divided into 5 groups(t =10 each):OGD group; 4％ sevoflurane postconditioning group(group Sevo); PD98059(specific inhibitor of ERK)group(group PD); dimethyl sulfoxide (DMSO)group; 4％ sevoflurane postconditioning + PD98059 group(group SPD).OGD was induced by incubating the slices in glucose-free aCSF aerated with 95％ N2-5％ CO2 for 15 min in group OGD.The hippocampal slices were perfused with aCSF saturated with 4％ sevoflurane for 30 min after OGD was induced in group Sevo.The hippocampal slices were perfused with aCSF containing PD98059 50 μmol/L for 10 min after OGD was induced in group PD.The hippocampal slices were perfused with aCSF containing DMSO 1 mol/L for 10 min after OGD was induced in group DMSO.The hippocampal slices were perfused with aCSF containing PD98059 50 μmol/L and aerated with 4％ sevoflurane for 30 min after OGD was induced in group SPD.The hippocampal slices were then perfused with plain aCSF for 1 h again in all the groups.The electrophysiological technique was used 1o record the amplitude of orthodromic population spike(OPS)in the stralum pyramidale of the CAI region.TTC staining was used to determine the degree of tissue injury.Results Compared with group OGD,the recovery amplitude and rate of OPS were significantly increased,and the degree of tissue injury was significantly decreased in group Sevo(P ＜0.01),while no significant change was found in each parameter in the other three groups(P ＞ 0.05).Compared with group Sevo,the recovery amplitude and tale of OPS were significandy decreased,and the degree of tissue injury was significantly increased in groups PD,DMSO and SPD(P ＜ 0.01).Conclusion ERK1/2 signal transduction pathway is involved in sevoflurane postcondilioning-induced reduction of OGD injury in tat hippocampal slices.

A total of 107 vitiligo patients were randomly divided into 3 groups.Group A received an intralesional injection of Bacillus Calmette-Guérin-polysaccharide nucleic acid (BCG-PSN) (n =34),group B Compound Glycyrrhizin Tablets (n =36) and group C both (n =37).Before treatment and 3 months after treatment,cellular immune function was detected for each group.Paired comparisons of 3 groups before and after treatment showed that CD4 +,CD4 +/CD8 + ratio increased (all P ＜ 0.05) and CD8 + decreased (P ＜0.05).After treatment,as compared with groups A and B,CD4 + increases (both P ＜ 0.05) and CD8 + decreased in group C (P ＜0.05).Group C had an efficiency rate of 91.9％ and it was higher than the other two groups (both P ＜ 0.05).An intralesional injection of BCG-PSN plus Compound Glycyrrhizin Tablets could improve immune function and treat vitiligo patients efficiently.