OBJECTIVETo observe the efficacy difference on the motor function in the children of cerebral palsy between acupuncture combined with acupoint embedding therapy and simple acupuncture.

METHODSOne hundred and twenty children of cerebral palsy were randomized into an observation group and a control group, 60 cases in, each one. In the observation group, acupuncture was applied in combination with acupoint embedding therapy. For the acupoint embedding therapy, the main acupoints were Pishu (BL 20), Shenshu (BL 23); the supplementary points were Dazhui (GV 14), Mingmen (GV 4), Yaoyangguan (GV 3), Sanjiaoshu (BL 22), Xinshu (BL 15) and Ganshu (BL 18). The catgut was embedded to the acupoints. Acupuncture was applied to the acupoints on the head and four limbs and the needles were retained for 40 min after qi arrival. In the control group, the simple acupuncture was applied, except for the acupoint embedding therapy. The rest treatment was the same as the observation group. The treatment lasted continuously for 3 months in the two groups. Before and after treatment, the gross motor function measure (GMFM) and the gross motor function classification system (GMFCS) were used for the assessment and the efficacy was analyzed.

RESULTS(1) After treatment, the total score of GMFM was increased in the two groups (both P<0. 01) and the score in the observation group was higher than that in the control group (P<0. 05). (2) The scores in A and B areas of GMFM were all improved in the two groups after treat ment (all P<0. 01) and the scores in the observation group were higher than those in the control group (P<0. 01 P<0. 05). (3) The total effective rate was 76. 7% (46/60) in the observation group, which was better than 55. 0% (33/60) in the control group (P<0. 01).

CONCLUSIONThe combined therapy of acupuncture and acupoint embedding much better improves the motor function in the children of cerebral palsy as compared with the simple acupuncture.

Acupuncture Points ; Acupuncture Therapy ; Catgut ; utilization ; Cerebral Palsy ; physiopathology ; therapy ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Motor Activity

Objective To investigate the clinical features, the video electroencephalography ( V-EEG) and synchronous electrocardiography ( ECG) changes in non-epileptic seizures and the significance of ECG monitoring in the diagnosis of epilepsy.Methods We collected 3 patients who came in a chief complaint of“episodes of unconsciousness with limbs twitch”, whose clinical features and EEG, ECG in longterm VEEG monitoring were analyzed.The 3 patients were followed up for at least 6 months.Results The 3 patients were all diagnosed as epilepsy in other hospital,and during the 24-hour VEEG monitoring in our hospital,2 patients showed abnormal cardiac rate and arrest during the attack and corresponding EEG changes after cerebral ischemia and hypoxia.After consultation with the department of cardiology, they were diagnosed as cardiac syncope episode and no attack showed up after the placement of pacemaker.The Q-T interval was prolonged in the other patient during the longterm ECG monitoring, after the coherence check who was diagonsed as severe hypocalcemia combined with myocardial damage due to a lower parathyroid function and had no attack after symptomatic treatment.Conclusions The synchronous ECG monitoring during the attack is of great significance in the differential diagnosis of epileptic and non-epileptic seizures. The ECG changes in patients with longterm VEEG monitoring should be focused on.It is necessary to add the early warning function to prevent the occurrence of accidents.

OBJECTIVE To investigate the effects of Anerdian(AnEr Iodine) combined with moist dressing on wound infection.METHODS Totally 124 patients with wound infection were divided into two groups,in the study group,the wounds treated by Anerdian combined with moist dressing.In the control group,the wounds were treated by traditional method.Their healing time was compared in the two groups.RESULTS The effects of wound healing in study group were much better than the control group(96.1% vs 44.7%,P

To study the role and molecular mechanism of epigallocatechin gallate (EGCG) in reversing drug-resistance to 5-fluorouracil (5-FU) in gastric cancer drug-resistant cell line SGC-7901/5-FU.

METHODSDrug-resistance gastric cancer cell line (SGC-7901/5-FU) was established by high doses of repeated impact joint drug concentration increment methods. The cell viability of the parent cell line and the drug-resistance cell line were determined by standard MTT assay. Cell survival rate of drug-resistance was calculated by the formula [(Aof the treatment group / Aof the control group) × 100%]. Cell half inhibitory concentration (IC) and resistance index (RI) were calculated by the Graphpad prime 6.0 software(RI=ICvalue of drug-resistance cells / ICvalue of parent cells). The apoptosis rate of SGC-7901/5-FU cells was quantified by flow cytometry after staining with annexin-V and PI. Western blot was used to detect the protein expression of drug-resistance-related proteins (ABCG2, P-gp, MDR-1 and GST-π) and apoptosis-related proteins (PARP, Survivin, Bax and bcl-2).

RESULTSICvalue was significantly increased in drug-resistant cells compared with parental cells [(64.7±3.9) mg/L and (4.1±0.3) mg/L, respectively, t=26.46, P=0.000], and the RI was 15.6. Proliferation activity in the drug-resistant cells was higher than that in parental cells at different 5-FU concentrations (all P<0.05). In drug-resistant cells, the ICvalue of 5-FU combined with EGCG group obviously decreased compared with 5-FU group [(7.3±0.1) mg/L and (63.1±1.4) mg/L respectively, t=40.84, P=0.000], and the RI was 0.12. Proliferation activity in drug-resistant cells was significantly decreased after EGCG treatment at different 5-FU concentrations (all P<0.05). Cell apoptosis rates in control group, 5-FU group, EGCG group and 5-FU combined with EGCG group were (3.0±1.0)%, (7.0±1.3)%, (6.0±1.2)% and (18.0±1.4)%, while apoptosis rate in 5-FU combined with EGCG group was significantly higher than those of other 3 groups(F=129.5, P=0.000). Western blot revealed that after EGCG treatment, the expression levels of drug-resistance-related proteins (ABCG2, P-gp, MDR-1 and GST-π) in the drug-resistant cell line SGC-7901/5-FU decreased significantly; the expression levels of apoptosis marker protein PARP and pro-apoptotic protein Bax increased significantly; and the expression levels of anti-apoptotic protein Survivin and Bcl-2 decreased significantly (all P<0.05).

CONCLUSIONEGCG can reduce the resistance of gastric cancer resistant cell line SGC-7901/5-FU, whose role may be via the inhibition of the expression of drug-resistance-related proteins, and the elevation of the protein expression ratio of PARP/Survivin and Bax/Bcl-2.

Anticarcinogenic Agents ; pharmacology ; Apoptosis ; Apoptosis Regulatory Proteins ; Catechin ; analogs & derivatives ; pharmacology ; Cell Line, Tumor ; Cell Proliferation ; Cell Survival ; Drug Resistance, Neoplasm ; Fluorouracil ; pharmacology ; Humans ; Stomach Neoplasms ; drug therapy ; pathology ; bcl-2-Associated X Protein

Humans ; Severe Acute Respiratory Syndrome ; diagnosis ; therapy