Aging is a complex pathological and physiological phenomenon characterized by the decline of many functions in human. Several lines of experimentation showed that the defect of the mitochondria function is one of the driving forces for the progress of aging. During the development of aging, the alteration of biology of mitochondria, the accumulation of the generation of "free radical"in mitochondria, the mutation in mitochondria genomes and the control of the progress of death by mitochondria were suggested to be a number of novel mechanisms for mitochondria in aging. The experiment data focused on the role of mitochondria in aging are reviewed.

Airway remodeling is one of the main pathological changes of bronchial asthma. It has shown that a variety of cytokines, including chemokines, growth factors, matrix proteins, participate in and even strengthen airway remodeling through building complex signal transduction pathways. Many traditional Chinese medicines,such as shegan mahuang decoction,have a regulatory role in airway remodeling.

Postural tachycardia syndrome (POTS) is one type of orthostatic intolerance.The treatment for POTS including non-drug treatment and medications,such as α-receptor agonists,β-recepter blockers and oral rehydration salts.The prognostic meaning of biomarkers and hemodynamic parameters in the POTS children treated with midodrine hydrochloride are discussed in this paper.

Objectives To explore the correlation between the heteroplasmy level of mt5178C＞A mutation in ND2 gene of mitochondria DNA and essential hypertension(EH)in middle-aged and elderly adults.Methods EH patients and normotensive controls were recruited consecutively from 2014-2015 from general population.Demographics,clinical characteristics and blood leukocytes were collected.The mt5178C＞A mutation heteroplasmy level was quantified by the rapid and sensitive realtime polymerase chain reaction(PCR) method for each participant.Results A total of 108 EH patients and 109 controls were recruited.The mt5178C＞A mutation heteroplasmy level was(42 ± 11％)in EH patients and (54± 13)％ in control subjects,with statistically significant difference between the two groups(P＜0.01).Using a two-step cluster analysis,the mt5178C＞A heteroplasmy level exceeding 44％ was associated with a decreased risk of EH(OR=0.18,95％,CI:0.10-0.31,P＜0.01).Correlation analysis showed mt5178C＞ A heteroplasmy level was significantly negatively correlated with both systolic blood pressure (r =-0.38,P＜ 0.001) and diastolic blood pressure (r =-0.49,P＜ 0.01)in 109 controls.Logistic regression analysis demonstrated that in single-factor analysis,mt5178C ＞ A heteroplasmy level (OR =0.82,95 ％ CI:0.77 0.87,P ＜ 0.01) was protective factor for EH,however,BMI(OR=1.30,95％CI:1.12-1.45,P＜0.01),total cholesterol(OR=2.13,95％CI:1.39-3.28,P=0.00),triglyceride(OR=7.62,95％CI:3.45-16.84,P＜0.01)and blood urea nitrogen(OR =1.35,95 ％ CI,P =0.03) were risk factors for EH.And a multiple logistic regression analysis showed that mt5178C＞ A heteroplasmy level (OR =0.83,95 ％ CI:0.78-0.89,P＜ 0.01) was independent protective factor for E H,however,only total cholesterol (OR =2.17,95 ％ CI:1.58-2.98,P =0.02) and low density lipoprotein cholesterol (OR =0.06,95％ CI:0.01-0.83,P =0.04) were independent risk factors for EH,and the P at critical 0.05 value.Conclusions Mitochondrial ND2 gene 5178C＞ A mutation heteroplasmy level exerts protective role against EH in middle-aged and elderly adults in Chinese population.

Objective To assess the relationship between eosinophil count and intracranial aneurysm (IA).Methods For the retrospective study,we collected data of the count of eosinophils obtained from the peripheral blood samples and other clinical data of 79 patients with IA admitted to our hospital between January 2014 and December 2015 and 65 healthy people as control group.Risk factors for IA were analyzed by multivariate Logistics regression analysis.Results Clinical characteristics were comparable between the two groups (P＞0.05).Compared with those in the control group,eosinophil and platelet counts in IA group were significantly higher [(0.18±0.12)109/L vs.(0.12±0.09)109/L;(196.44±57.33)109/L vs.(178.80±47.23)109/L,respectively;all P＜0.05],while platelet distribution width (PDW) and mean platelet volume (MPV) were lower [(13.95 ± 3.332)fL vs.(15.30±3.5)fL;(11.02±1.73)fL vs.(11.66±1.31)fL,respectively;all P＜0.05].Logistic regression analysis indicated that eosinophil was the independent risk factor for IA.Corclusion Our study demonstrated that increased eosinophil count is the independent risk factor for IA.

With social progress and improvement of human health consciousness,the medical device industry of China has been fast developing,and a new request for the specialized talents cultivation has been put forward.Based on the consideration of the development of medical device industry and the students,a cultivating mode for the medical engineering talents is explored in this paper through the major course reform,training double-quality teachers and cooperation between industry,university and research institutes.

Objective To investigate the effect of continuous health education on the control of glucose and the changes of behavior. Methods One hundred and twenty-six newly diagnosed type 2 diabetic patients were randomly divided into control group and observation group,each group included 63 patients. The control group just received health education in hospitalization period,and the observation group received continuous health education both in and after hospitalization. The control of glucose and the changes of behavior were evaluated 3 months and 1 year later. Results After 3 months and 1 year treatment,the FPG,2hPG and HbA1c were significantly decreased compared to pre-treatment both in control group and observation group(P<0. 05). The FPG,2hPG and HbA1c were significantly decreased in the ob-servation group compared to the control groups(P<0. 05). The behaviors contained that mastery of diabetic knowledge,control of food in-take,persist in physical exercise, rational administration, self-monitoring of blood glucose and regular reexamination were significantly im-proved both in control group and observation group compared to pre-treatment (P<0. 05),and the behaviors were significantly improved in the observation group compared to the control group (P<0. 05). Conclusion Compared to control group,observation group had more ad-vantages in the control of glucose and the changes of behavior in newly diagnosed type 2 diabetic patients.

Principles of medical equipment course were explored of formative evaluation as the center examination,constructed an examination reform scheme as” One center (formative evaluation as the center),three systems (content system,operation system,monitoring system).” Made students to learn from the center of focus scores change to autonomous learning and culture analysis,solves the question ability and practical ability.At the same time,strengthen the teaching process of teaching and learning to communicate and exchange,Get the students learning feedback information to improve teaching and learning in time,Strengthen the students′ ability of autonomous learning and lifelong learning ability.

Medical treatment technology has made a lot of breakthrough because of the rapid development of the medical devices industrial.Minimally-invasive and noninvasive medicine is the development tendency of the future medicine.To adapt to the needs of the future medicine,Chongqing medical university exerted much efforts on training of minimally-invasive and noninvasive medical talents.We explored and practiced in aspects of fixing educational conception and goal,building curriculum and teaching content,constructing professional teaching team and developing practice and innovation abilities in order to fill the void of personnel training in the minimally-invasive and noninvasive medical field.

Objective To investigate the effect of suppressor of cytokine signaling-1 (SOCS-1)on expression of monocyte chemoattractant protein-1 (MCP-1)in human glomerular mesangial cells (HMCs) under high concentration of glucose. Methods Stable transfections of HMC with pCR3.1 vector and pCR3. 1-SOCS-1 were performed with hpofectamine 2000, and cells were selected with geneticin. Cells were stimulated with low glucose (LG, 5.5 mmol/L), high glucose (HG, 30 mmol/L), LG plus mannitol (24.5 mmol/L) and AG490 (10 μmol/L). The protein expression levels of SOCS-1, signal transducer and activators of transcription 1,3 (STAT1, STAT3),p-STAT1 and p-STAT3 were observed by Western blotting. The protein synthesis of MCP-1, FN and type Ⅳ collagen in the supernatants of the HMCs were detected by ELISA and radioimmunoassay. The expression level of SOCS-1 and MCP-1 mRNA was measured by BT-PCR.Results HG induced the expression of SOCS-1 protein and mRNA in HMCs in time-dependant manner, peaked at 4 h, and gradually decreased to baseline at 24 h. Compared with low glucose control group, the phosphorylation levels of STAT1 and STAT3 and the expression of MCP-1 mRNA [(0.39±0.05) vs (0.16±0.02)]were significantly increased in HMCs under high glucose medium (P ＜0.01 ). Exposure of HMCs to high glucose conditions showed high concentration of MCP-1 [(459±67) ng/L vs (241±19) ng/L], fibronectin [(5.84±0.61) mg/L vs (3.41±0.31) mg/L]and type Ⅳ collagen [(16.45±2.30) μg/L vs (9.56±1.52) μg/L]in the supernatants (all P＜0.01).Overexpression of SOCS-1 inhibited the phosphorylation levels of STAT1 and STAT3 and the expression of MCP-1 mRNA [(0.34±0.04) vs (0.42±0.05)]in HMCs under high glucose condition (all P＜0.05). Compared with vector control group, the concentration of MCP-1 [(387±47) ng/L vs (463±56) ng/L], fibronectin [(4.61±0.57) mg/L vs (5.76±0.74) mg/L]and type Ⅳ collagen [(13.4±2.32) μg/L vs (17.1±2.57) μg/L]was decreased in supernatants of HMCs with SOCS-1 overexpression (all P＜0.05). Compared with HG group, the expression of MCP-1 mRNA (0.31±0.04) and the concentration of MCP-1 [(361±53) ng/L], FN [(5.46±0.71) mg/L]and type Ⅳ collagen [(15.2±1.97) μg/L]in supernatants were decreased in HMCs treated with AG490.Conclusion Overexpression of SOCS-1 inhibits overproduction of MCP-1 and ECM proteins in HMCs under high glucose conditions, which may be partly by regulating the phosphoryhtion of STAT1 and STAT3.