1.Determination of the Content of Emodin in Qinhuang Houzhen Capsule by HPLC
Traditional Chinese Drug Research & Clinical Pharmacology 1993;0(03):-
Objective To determine the content of emodin in Qinhuang Houzhen Capsule and to provide evidence for its quality criteria.Method A HPLC method was established as follows: Dionex AcclaimTM120 C18 cloumn(4.6? 150 mm,5 ? m),methnol-0.4 % phosphoric acid aqueous solution(85∶ 15) as mobile phase,flow rate at 1.0 mL/min and detection wavelength at 254 nm.Results The average content of emodin in Qinhuang Houzhen Capsule was 0.2057 mg/g,and RSD=4.11 %.Conclusion The content of rhubarb calculated by emodin in 1 g Qinhuang Houzhen Capsule could be affirmed at 0.150 mg/g tentatively.The method is simple,acurate,repeatible,and can be used for the quality control of Qinhuang Houzhen Capsule.
2.Influence of Gamma-rays Radiation Sterilization on the Content and Fingerprint Chromatography of Effec-tive Constituents in Simiao Junyi Ointment
Qun HE ; Guangyu CHEN ; Shi WANG ; Siyuan PENG ; Yongheng HE ; Kun LUO
China Pharmacist 2017;20(3):405-408
Objective:To explore the degradation effect of gamma-rays radiation sterilization on the effective constituents in Simiao Junyi ointment and study the changes of fingerprint chromatography before and after the sterilization to provide basis for the feasibility of gamma-rays radiation sterilization for Simiao Junyi ointment. Methods:The contents of tetrahydropalmatine and PNS ( Panax Notogin-seng saponins) in Simiao Junyi ointment were determined by HPLC, and the fingerprint chromatography was established. The content changes of tetrahydropalmatine and PNS in Simiao Junyi ointment before and after the gamma-rays radiation sterilization were compared among the same batch and various batches, and the relative retention time and relative peak area in the fingerprint chromatography were also compared. Results:The content of tetrahydropalmatine had no change basically before and after the gamma-rays radiation steriliza-tion(P >0. 05), and there was no change in the total content of PNS (P >0. 05). Comparing the HPLC fingerprint chromatography at 280 nm, the relative retention time had statistically significant change after the gamma-rays radiation sterilization ((P <0.05), while the relative peak area had no statistically significant change(P >0. 05). The number of characteristic peaks reduced by one, namely the C8 characteristic peak disappeared in the pasteurized chromatography, and the areas of C6, C9 and C14 peak decreased significantly, while that of C12 increased. Conclusion:Gamma-rays radiation sterilization have no notable effect on the content of tet-rahydropalmatine and PNS in Simiao Junyi ointment, it can be used for the sterilization of Simiao Junyi ointment.
3.Role of functional magnetic resonance imaging in evaluating the therapeutic effect of transcatheter arterial chemoembolization in hepatocellular carcinoma
Journal of Clinical Hepatology 2016;32(12):2277-2281
Transcatheter arterial chemoembolization (TACE) is the first-line treatment for unresectable hepatocellular carcinoma (HCC). Postoperative follow-up helps to determine tumor survival and necrosis timely and accurately and plays an important role in evaluating therapeutic outcome, guiding future treatment, and reducing pain. This article introduces the application of functional magnetic resonance imaging, including magnetic resonance spectroscopy, magnetic resonance perfusion weighted imaging, diffusion weighted magnetic resonance imaging, intravoxel incoherent motion, and blood oxygen level-dependent magnetic resonance imaging, in observing the changes in metabolism, blood flow, energy, and blood oxygen in tumor cells and points out that functional magnetic resonance imaging has promising prospects in evaluating the therapeutic effect of TACE in HCC.
4.Rapid Identification and Quality Analysis of Olibanum by TGA-DTA Thermal Analysis
Yongheng WEI ; Zhiquan ZHENG ; Juyuan LUO ; Guohang JIN ; Gongsen CHEN ; Yanni LI ; Xinjie LI ; Jinli SHI
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(4):629-635
This study was aimed to quickly identify Chinese medicine Olibanum.Thermal analysis method was used on the quality analysis of Chinese materia medica (CMM).A total of 25 batches of Olibanum on the market were collected.This study examined three important factors of temperature range,heating rate,powder mesh on the TGA and DTA thermal analysis experiments.And a method of rapid authentication of medicinal materials using TGA and DTA feature maps was built.Methods of the first-order points,connection on thermogravimetric analysis and heat enthalpy calculation were adopted in the quantitative analysis of Olibanum.The results showed that the best condition of TGA and DTA experiment on Olibanum was confirmed.The temperature range is 50-750℃.The heating rate is 20℃· min-1.The powder mesh is 100 mesh.Under these conditions,good quality goods of Olibanum,counterfeit Olibanum and adulterants of Olibanum could be distinguished through the characteristic peak (T1=447 ± 5℃,T2=549 ± 5℃,T3=350 ± 5℃),thermogravimetric analysis (TV-max,△W2+△W3) and thermal enthalpy analysis (△H).It was concluded that the TGA-DTA technology was simple.It was thought to be a rapid,accurate and simple new method for Olibanum identification and quality analysis.
5. Applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou
Yanshan CAI ; Yuzhou GU ; Fei ZHONG ; Lirui FAN ; Yuteng ZHAO ; Yefei LUO ; Yongheng LU ; Weiyun HE ; Weibin CHENG ; Huifang XU
Chinese Journal of Epidemiology 2019;40(10):1212-1216
Objective:
This article was to evaluate the applicability and feasibility of "Lingnanzhun" -an "Internet Plus-based HIV Self-testing Tool" targeting MSM in Guangzhou. Hopefully, the results could be used to improve the existing HIV testing services and to support the implementation and scale-up of HIV self-testing programs.
Methods:
Data were collected from a survey on HIV testing preferences among the Internet-using MSM in April to June, 2015. Univariate and multivariate logistic regression analysis were applied to identify factors associated with the use of HIV self-testing service provided by Lingnanzhun. Information related to the users of Lingnanzhun during September 2014 and December 2018 was also collected.
Results:
769 MSM were recruited as participants. Of them, age distribution was 16-77(28.6±6.8) years old, 88.3
6. A new method measuring the rotation center of hip joint and its significance for hip replacement surgery
Liwen ZHENG ; Wanchun WANG ; Zhihong LI ; Tang LIU ; Yongheng LUO ; Lei WANG ; Ren WU ; Guoliang HUANG ; Ding LI ; Shuo JIE
Journal of Chinese Physician 2019;21(11):1626-1631,1635
Objective:
To design and evaluate a new method for measuring the center of rotation of the hip joint, and to compare it with the traditional methods.
Methods:
Data of healthy hips of 120 total hip arthroplasty (THA) patients (120 hips) and bilateral hips of 20 healthy volumteers (40 hips) were collected. A series of X-rays and magnetic resonance imaging (MRI) were taken from each patient with the hip joint abductted at differenct angles (0°, 7.5°, 15°, and 22.5°). The motion track of rotation center was obtained by comparing these X-ray and MRI images. The preoperative and postoperative data of 67 surgical patients who underwent two different measurement methods before surgery were compared.
Results:
Compared with traditional measurement methods, the accuracy of our new method was significantly improved; the results of hip MRI verified the objectivity and accuracy of our new method; preoperative measurements and postoperative follow-up results showed that our new method had a better clinical effect than traditional measurements.
Conclusions
The team′s innovative new measurement method based on pelvic orthotopic X-ray photographs with different abduction angles of the hip shows more accurate data than traditional measurement methods and shows better clinical results.