OBJECTIVE:To study the effects of Cornus officinalis polysaccharide on learning and memory ability of vascular dementia (VD) model rats and its mechanism. METHODS:30 rats were randomly divided into sham operation (normal saline) group,model(normal saline)group and C. officinalis polysaccharide(0.28 g/kg)group. Except sham operation group,VD model was induced in other groups and given relevant medicine intragastrically once a day. After 4 weeks,the frequency of leaping over original platform position,water maze mean escape latencies,platform retention time and average swimming distance were detect-ed,and the mRNA and protein expression of brain-derived neurotrophic factor(BDNF)and apoptosis related gene Bcl-2 in hippo-campal tissue of rats were determined. RESULTS:Compared with sham operation group,learning and memory ability of rats in model group weakened,manifesting as the frequency of leaping over original platform position decreased,water maze mean escape latencies prolonged,platform retention time shortened,average swimming distance extended(P<0.05);mRNA and protein expres-sion of BDNF and Bcl-2 in hippocampus decreased significantly (P<0.05). Compared with model group,learning and memory ability of rats in C. officinalis polysaccharide group increased,manifesting as the frequency of leaping over original platform posi-tion increased,water maze mean escape latencies shortened,platform retention time prolonged,average swimming distance short-ened(P<0.05);mRNA and protein expression of BDNF and Bcl-2 in hippocampus was enhanced significantly(P<0.05). CON-CLUSIONS:C. officinalis polysaccharide can improve the learning and memory ability of VD rats,and its mechanism may be asso-ciated with the expression up-regulation of BDNF and Bcl-2 in hippocampus.

The status and disadvantage on Information System of Battlefield Blood Supply are analyzed.By combining with practical condition,the adaptation future war information system of war blood supply based on Beidou Navigation Satellite is designed.The system aims at demand of present military,and goal for improving the ability of blood supply and promot-ing informationization level of blood management in battlefield.The advanced computer technology,communications tech-nology,satellite navigation technology and integrating principle are adopted.It is practicality exploration in informatization construction of war blood supply.

Rough set is a mathematical approach to study imprecise, uncertain or incomplete information or knowledge. The data mining technology based on the rough set theory can be trained through the training data set. The technology is applied to such fields as the prognosis of the pain in the chest, emergence diagnosis in ICU, distinguishing and diagnosis of lung tumor and rheumatoid disease, diagnosis of galactophore disease, diagnosis of premature birth, classification and diagnosis of liver disease and acute appendicitis. The technology has a brilliant future in medical data mining.

As the head transplant program“Heaven Surgery” was put forward, there was birth to many bioethi-cal confusion, clinical-medical ethical confusion and other questions of ethical confusion. Face with various ethi-cal confusions, clinician need rational return of humanism, and the public need pay more attention to the essence of life and the concept of life and death.

Thin endometrium is an important factor influencing the in vitro fertilization and embryo transfer, and there is a lack of effective therapy in the treatment of thin endometrium.The aim of this study was to explore a stable animal model of effective thin endometrium, and to promote the research on thin endometrium pathogenesis and provide experimen-tal basis of treatment.To analyze a variety of establishments of endometrial damage animal model reported in the domestic and foreign literatures,it is concluded that perfusing 95% ethanol into uterine cavities of rats can establish a rat model of thin endometrium,and put forward some experience and methods for its improvement.

Objective The purpose of this study was to compare the adenomyosis models in nude mice generated by four different methods,and to find out an optimal modeling method, and to provide an ideal animal model for exploring pathogenesis and experimental treatment of uterine adenomyosis. Methods 1. 80 female healthy nude mouse were divided randomly into 4 groups: Intraperitoneal implantation group, subcutaneous implantation group, intraperitoneal injection group, and subcutaneous injection group. The transplants were taken for pathological examination at 4 weeks after surgery. Results The success rate of intraperitoneal implantation group was 95%,and that of the subcutaneous implantation group was 45%,while the success rate of intraperitoneal injection group and subcutaneous injection group was 0%. Conclusions Establishment of a nude mouse model of uterine adenomyosis by intraperitoneal implantation method has a high success rate and with good stability, and is an ideal mouse model of human-derived uterine adenomyosis.

Objective To observe the effect of Juli Sanjie Pill(JSP) on estrogen receptor(ER)? and ER? expression in the myometrium and hysteromyoma tissue,and to explore the correlation of ER? and ER? expression levels with the incidence of hysteromyoma. Methods Forty hysteromyoma patients were divided into two groups: 20 patients with operative indications after oral use of JSP and asking for operation,were enrolled in the treatment group,and other 20 patiens without mediation of medicine but asking for operation were in the control group.The ER? and ER? expression levels in hysteromyoma tissue and the surrounding normal myometrium of the two groups were detected by radioimmunoassay.Results There presented the expression of ER? and ER? in the hysteromyoma tissue and the surrounding normal myometrium of the two groups,and the levels in the hysteromyoma tissue were higher than that in the myometrium(P0.05).Conclusion The incidence of hysteromyoma is correlated with the local expression of ER? and ER? in the uterus.The therapeutic mechanism of JSP for hysteromyoma is probably related with the decrease of ER? and ER? expression levels in hysteromyoma tissue and with the decrease of ER? level in the surrounding normal myometrium.

Several critical information technologies are discussed, which will deeply impact the informatization construction and medical support of the field hospital. The importance and development trends of these technologies in military medical service are also analyzed. These technologies will be an indispensable part in military informatization.

OBJECTIVE:To study the bio-equivalence of domestic loratadine syrup and tablets in human beings.METHODS:a dose fo40mg domestic loratadine(syrup or tablet)was given to18healthy male volunteers to a randomized crossover design.Blood samples were collected before administration and20min,40min,1hr,1.5hr,2hr,3hr,4hr,6hr,8hr,12hr,24h after administration.The concentrations of Loratadine in blood were determined by HPLC.Pharmacokinetic parameters and relative bio-availability were detected with3p97program.RESULTS:The main pharmacokinetic parameters of domestic Lortadine syrup and tablet were as follows:C max were(40.91?15.42)ng/ml and(41.57?18.68)ng/ml respectively;T max were(1.04?0.19)h and(1.19?0.25)h respectively;T 1/2 were(4.43?1.67)h and(4.21?1.49)h respectively;AUC 0～24 we_ re(127.60?46.28)(ng?h)/ml and(133.13?45.65)(ng?h)/ml respectively;AUC 0～∞ were(132.98?47.43)(ng?h)/ml and(138.16?47.26)(ng?h)/ml respectively.The relative bio-availability was(96.25?21.30)%.CONCLUSION:the do?mestic Lortadine syrup and tablet are bio-equivalent.

Objective:To compare the difference on the positive detection rate of the main aeroallergens and food allergens specific IgE measured with the systems between fluorescence enzyme immunoassay(FEIA, referred to as ImmunoCAP system)and western blot(referred to as Allergy Screen system), in order to provide a basis for the rational application of methods and interpretation of the test results.Methods:The clinical information and sIgE test results data were collected from a total of 458 cases of allergic diseases from October 2017 to April 2019 in the outpatient clinic of Allergy Department in Beijing Children′s Hospital.All of the 458 cases were detected for a panel of common aeroallergens and food allergens sIgE level in their serum with the Allergy Screen system.Simultaneously, the above 141 cases were detected main aeroallergen and food allergen sIgE with ImmunoCAP system, while 303 cases only for aeroallergens and 14 cases only for food allergens.All of the cases were divided into three different phenotype groups according to the main target organ and diagnosis such as airway allergic disease group( N=293), skin allergic disease group( N=14)and multi-system allergic disease group( N=151). Meanwhile, three different age groups were referred to as <3 years old group( N=97), 3 to 6 years old group(3 years and 6 years included)( N=186)and >6 years old group( N=175). The same kinds of the allergens included in the two systems were house dust mite(HDM), cat dander, dog dander, egg white, milk, crab, shrimp, therefore data of sIgE to those seven allergens were compared. Results:In all the enrolled cases, the positive detection rate of HDM, egg white and milk sIgE detected by ImmunoCAP system were significantly higher than those by Allergy Screen system(30.6%, 36.1% and 43.2% vs 21.2%, 21.3% and 21.3%). Among the disease groups, the positive detection rate of HDM sIgE detected by ImmunoCAP system was significantly higher in the airway allergic disease group and multi-system allergic disease group than those by Allergy Screen system, respectively(33.7% and 24.6% vs 23.4% and 16.2%). The positive detection rate of egg white and milk sIgE detected by ImmunoCAP system in the multi-system allergic disease group was significantly higher than those by Allergy Screen system, respectively(47.6% and 47.6% vs 26.2% and 25.2%). There was no significant difference in the positive detection rate of cat dander, dog dander, crab and shrimp sIgE among the disease groups.The positive detection rate of HDM sIgE detected by ImmunoCAP system in all of the age groups were significantly higher than those by Allergy Screen system, respectively(14.9%, 26.9% and 42.3% vs 5.7%, 17.6% and 32.6%). The positive detection rate of cat dander sIgE detected by ImmunoCAP system in <3 years old group was significantly lower than that by Allergy Screen system(6.9% vs 16.1%). The positive detection rate of egg white sIgE detected by ImmunoCAP system in the <3 years old group and the 3 to 6 years old group were higher than those by Allergy Screen system, respectively(47.1% and 33.3% vs 32.4% and 15.0%). The difference in the positive detection rate of milk sIgE detected by these two methods in the <3 years old group and the 3 to 6 years old group was similar to the egg white.Conclusion:According to the analysis of seven kinds of major aeroallergen and food allergen sIgE results, there are differences between the two methods to detect HDM, cat dander, egg white and milk sIgE.It depends on the type of allergic disease and age.The positive detection rates of dog dander, crab, and shrimp sIgE detected by the two methods are consistent.In clinical application, comprehensive analysis should be made, testing methods should be selected rationally, and the results should be interpreted scientifically.