NF-κB is a key regulator in inflammation-associated tumors. Constitutively activated NF-κB participates in the development of many hepatitis virus-associated cancers(hepatocellular carcinoma, cholangiocarcinoma). Inhibition of NF-κB's activity can effectively slow or stop cancer growth and metastasis and enhance cancer cells' sensitivity to chemotherapy. Thus, NF-κB may become an effective therapeutic target in hepatitis virus-associated cancers in the near future.

OBJECTIVE: To explore the correlation between the severity of patients with rhinitis-nasosinusitis and the bronchial asthma and the pulmonary function. METHOD: Sixty-four cases of patients with rhinitis-nasosinusitis and 53 cases of patients with chronic sinusitis from June 2012 to September 2013 were randomly selected, and the patients were divided into group of rhinitis-nasosinusitis with nasal polyps and group of chronic sinusitis according to disease species, and analyzed the correlation between the severity of the deseases and the changes of the pulmonary function respectively for the patients of two groups by using Spearman method. RESULT: The incidence of asthma for patients with different levels of sinusitis and nasal polyps seemed no significant difference (P > 0.05); the incidence of allergic rhinitis also seemed no significant difference (P > 0.05); the incidence of asthma for patients with different lesion range of sinusitis and nasal polyps seemed no significant difference (P > 0.05); the incidence of allergic rhinitis also seemed no significant different (P < 0.05). The conditions of the patients with different levels of sinusitis and nasal polyps were directly related to the reduction of pulmonary function (r = 2.431, P < 0.05); The conditions of the patients with different lesion range of sinusitis were directly related to the reduction of pulmonary function (r = 2.641, P < 0.05). CONCLUSION There was some correlation between the severity of patients with rhinitis-nasosinusitis and the bronchial asthma and the condition of pulmonary function of patients.
Asthma ; physiopathology ; Chronic Disease ; Humans ; Lung ; physiopathology ; Nasal Polyps ; physiopathology ; Respiratory Physiological Phenomena ; Rhinitis ; physiopathology ; Sinusitis ; physiopathology

BACKGROUND:The traditional repair methods for laryngeal cartilage defect are restricted by donor source, rejection, which are difficult to be popularized. OBJECTIVE:To investigate the effect of polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold in repair of laryngeal cartilage defect. METHODS: A total of 20 Wistar rats were randomly divided into polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold and polylactic acid-glycolic acid copolymer scaffold groups. Polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold and polylactic acid-glycolic acid copolymer scaffold were respectively used for repair after the establishment of laryngeal cartilage defect models. RESULTS AND CONCLUSION: The laryngeal cartilage defect diameter of rats at 3, 5 and 7 days after modeling in the polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold group was significantly smaler than that in the polylactic acid-glycolic acid copolymer scaffold group. The laryngeal cartilage defect of rats in the polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold group was basicaly repaired and presented with a smooth surface, and there were no clear boundaries with surrounding tissues; however, the laryngeal cartilage defect of rats in the polylactic acid-glycolic acid copolymer scaffold group had indentations with a rough surface, and there were obvious boundaries with surrounding tissues. These results demonstrate that polylactic acid-glycolic acid copolymer/hydroxyapatite composite scaffold can promote the repair of laryngeal cartilage defect part, and its repair effect is more ideal.

Objective:To evaluate the effects of different ultrasonic retroprep tips on the root-end preparation in cadavers.Methods:34 anterior teeth in situ in maxillary and mandibular jaws in cadavers were chosen and the root-end of 3 mm of all teeth was exposed. Root canal was prepared and filled with gutta-purcha in all teeth,3 mm of the root end was cut off.The teeth were divided into 2 treat-ment groups(n=1 5),and 1 control group(n=4).The root-end preparation was made using ultrasonic diamond tip and stainless steel tip respectively to 3 mm depth.The teeth in control group were not treated.Microcracks,marginal integrity and gutta-percha clearance were observated under SEM.Results:Diamond ultrasonic tips provoked a smaller number of cracks(P=0.059)and destroy of margin-al integrity(P=0.269)than stainless steel tips.Less gutta-percha clearance was produced by stainless steel tip than that by diamond ultrasonic tip(P=0.006).Conclusion:Diamond ultrasonic tip is more suitable for root-end preparation.

Na +/H + exchangers(NHE) are vital transmembrane proteins involved in multiple cellular functions including the regulation of intracellular pH, the control of cell volume and the onset of proliferation, differentiation and apoptosis of cells, hence associated with liver fibrosis due to mediating hepatic stellate cell(HSC) activation and collagen synthesis effects induced by cytokines and oxidative stress. NHE is expected to be a new important target for antihepatofibrosis.

AIM: To investigate the effect of antioxidant curcumin on the lipid peroxidation of oxidative stress in rat hepatic stellate cell in vitro. METHODS: HSC T6 were incubated with 0.1mM ferric nitrilotriacetate complex (FeNTA), MTT colorimetry was used for assaying proliferation of HSC, Cytotoxicity was measured by LDH colorimetry, Collagen type Ⅰ accummulation in the culture media was measured by ELISA, Intracellular malonildialdehyde (MDA)、SOD、GSH and GSH PX levels in the culture media were measured by their reagent boxes. RESULTS: HSC incubation with FeNTA resulted in a significant production of intracellular MDA and GSH, associated with decrease SOD and GSH PX activity. Exposure of HSC to FeNTA significantly enhanced the number of proliferating HSC and collagen type Ⅰ levels in the culture medium. All these effects were reversed by the antioxidant curcumin. CONCLUSION: The mechanism of curcumin on fibrosis may be related to inhibit lipid peroxidation

Objective:To measure the expression of the MCP-1(Monocyte Chemoattractant Protein-1) in the serum of patients with acute spinal cord injury and explore the possible mechanism of secondary spinal cord injury.Methods:MCP-1 in the serum of patients with acute spinal cord injury,single spine compression and healthy subjects were detected by ELISA irrespectively.The MRI data of these patients were studied at the same time on a blind base.Results:MCP-1 in the serum of patients with acute spinal cord injury was correlated positively with the degree of spinal cord compression,which was elevated markedly(P

BACKGROUND: Studies in recent years have suggested the involvement of chemoattractant cytokines in the recruitment of peripheral blood cells to the injured spinal tissue. Monocyte chemoattractant protein-1(MCP-1) belongs to the CC-type chemokines and is capable of specific chemotactic attraction of the macrophages.OBJECTIVE: To observe MCP-1 expression in the serum of patients with acute spinal cord injury and explore the possible mechanism of secondary spinal cord injury.DESIGN: A non-randomized and controlled concurrent pilot study.SETTING and PARTICIPANTS: Eight patients with acute incomplete spinal cord injury and 8 with compression fracture of the spine were treated in the Department of Orthopaedics, Renmin Hospital of Wuhan University during the period from January 2001 to December 2002. Another 8 healthy subjects were included as the controls.METHODS: In the next morning after hospitalization, totally(8-10) mL of fasting peripheral venous blood was collected from the patients and the serum separated for determination of MCP-1 level with enzyme-linked immunosorbent assay(ELISA) . Serum MCP-1 level was also measured in the healthy subjects in the same manner.MAIN OUTCOME MEASURES: Serum level of MCP-1 in each group.RESULTS: Serum levels of MCP-1 in the healthy subjects, patients with compression fracture of the spine and those with acute incomplete spinal cord injury were(124 ± 15), (184 ±21) and(428 ± 11) ng/L, respectively, with significant differences between any two groups( P ＜ 0.01 ).CONCLUSION: MCP-1 may induce secondary inflammation by recruiting inflamnatory cells to the injury site and thus aggravate the spinal cord injury.

The effect of the selective debridement with the aid of the arthroscope performed under local anesthesia for osfeoarthritis of 296 knees in 260 patients was studied. The pathological changes of osteoarthritis under the arthroscopy include hyperplasia of synovial membrane, degeneration of cartilage or exposure of subchondral bone on the stress areas of the femur condyles and patella , wear and tear of meniscus, formation of osteophyte and loose body. The debridement included the removal of the loose body, hyperplasia of synovial membrane, degenerated cartilage, and the torn meniscus. Many factors,including age of the patients, selection of the indication, stress of the knee joint, and the degeneration of cartilage,may influence the curative effect. After selecfive debridement and lavage for osteoarthritis of the knee joint, the majority of patients were released from pain, with restoration of function and range of motion, and the development of pathological process was retarded. Good and excellent results constitute 74.7% of the patients.

Objective:To guide antithrombotic medication in the patients with PCI by GRACE and CRUSADE risk score. Meth-ods:The GRACE and CRUSADE score at the admission of 147 patients with ACS undergone PCI in one 3-level hospital were analyzed, and the two risk scores of the PCI patients and the correlation between the two scores were studied. Results:The risk factors in the two scores were part of the same, and the two scores had significant positive correlation. Conclusion: The GRACE score and CRUSADE score should be assessed at the same time in clinics to weigh the risk of bleeding and ischemia fully and then develop strategies for an-tithrombotic treatment.