Objective To evaluate the effectiveness and predictive value of high-resolution manometry(HRM) for POEM in treating achalasia. Methods A total of 84 achalasia patients categorized into subtypes by HRM, who also underwent POEM, were enrolled in our study. Eckardt score, Barium esophagogram and HRM were performed before, 6 months and 1 year after POEM. Results POEM was successfully performed in all 84 patients. No perforation occurred in any patient. The Eckardt scores and esophageal diameter after POEM significantly reduced compared with those before(P<0. 05). The 4s-IRP decreased from 33. 4±9. 0 mmHg (1 mmHg =0. 133 kPa) to 14. 6±3. 8 mmHg six months after POEM (P<0. 05) and to 16. 4±3. 9 mmHg one year after POEM (VS preoperate, P<0. 05). The LESP before treatment was 41. 8±15. 4 mmHg, decreasing to 18. 4±7. 1 mmHg six months after POEM (P<0. 05) and 20. 7±7. 6 mmHg one year after POEM (VS preoperate, P <0. 05) . When categorizing patients into 3 subtypes by HRM, 4s-IRP of type II showed the most dramatic decrease six months after POEM(62. 8%), followed by typeⅠ(53. 5%), while type III had the least decrease(41. 8%). The mean decreasing rate of LESP in type III was 42. 3% six months after POEM, followed by typeⅠ(55. 3%) , while type II showed the highest rate(57. 8%). Conclusion POEM is a safe treatment for achalasia and has significant short-term efficacy with Type II responding best to POEM. HRM plays a vital role in typing AC and predicting the effectiveness of POEM and can be useful in selecting an appropriate treatment.

Objective To cultivate and breed laboratory cats in conventional laboratory animal facilities, collect background data on laboratory cats, and compare them with purchased domestic cats to assess the feasibility of breeding laboratory cats. Methods Indigenous cat breeds were introduced for reproduction and population expansion under conventional laboratory environment, with recording of kitten survival rates and growth curves. Indicators of 20 laboratory cats of F1 generation (half male and half female), including complete blood count, blood biochemistry, organ mass, organ coefficient, heart rate, and blood pressure, were detected and comparisons between sexes were made. Blood pressure values and sensitivity to histamine of these cats were measured using depressor substance detection method in the Pharmacopoeia of the People's Republic of China-Four Parts: 2020, and were compared with the data from 173 concurrently purchased domestic cats. Results Laboratory cats adapted well to the environment of conventional laboratory facilities, with a survival rate of 77.08% of kittens at 8 weeks of age. Red blood cell count, hemoglobin content, mean corpuscular hemoglobin concentration, and hematocrit in male laboratory cats were significantly higher than those in females (P<0.01), while the mean corpuscular volume in males was significantly lower than that in females (P<0.01). The levels of serum alanine aminotransferase, total bilirubin, creatinine, triacylglycerol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol in male laboratory cats were significantly higher than those in females (P<0.05 or P<0.01), while cholesterol, globulin, total protein, and the albumin-globulin ratio were significantly lower in males (P<0.01). The liver coefficient in male laboratory cats was significantly lower than that in female cats (P<0.05), while the kidney coefficient was significantly higher (P<0.05). The spleen-brain and kidney-brain ratios were significantly higher in males compared to females(P<0.05 or P<0.01). No significant differences were found in heart rate, systolic pressure, diastolic pressure, mean blood pressure, or sensitivity to histamine between male and female laboratory cats (P>0.05). Compared to laboratory cats, purchased domestic cats had significantly higher heart rate, systolic pressure, and mean blood pressure (P<0.01), and the magnitude of blood pressure changes induced by medium and high doses of histamine was significantly reduced (P<0.05 or P<0.01). Conclusion It is feasible to breed laboratory cats in conventional laboratory animal facilities. The accuracy of experimental results can be improved by using laboratory cats with clear and standardized background data.