AIM To study the effect of antisense oligonucleotide s of telomerase on the telomerase activity and the mechanisms of its inhibiting cervical carcinoma HeLa cell line proliferation. METHODS The cytotoxic effect in vitro was determined using the trypan blue dye exclusion assay. The telomerase activity was performed by PCR-TRAP and the flow cytometry analysis was employed to investigate the effects of ASON on the cell cycle and apoptosis. RESULTS There was no significant decrease in cell viability after treated with ASON for 5 days and the ASON with FuGENE6 reduced the telmerase activity compared with the tandem primer and the empty controls. The efficancy depended on the dose of ASON and the time of treatment. ASON did not induce cell apoptisis but increased G 2/M-phase cells. CONCLUSION Antisense oligonucletides of telomerase inhibited the telomerase activity but did not induce apoptosis in HeLa cell lines .Retardation of G 2/M-phase may be the mechanism of inhibiting proliferation in HeLa cell lines.

The extracts of marine mollusks( Abalone, Aplysia, Sepia, Arcagranosa linnaeus, Cyclina sinensis, Myti-lus coruscus, Chlamys farrei, Meretrix meretrix, Ostrea, Sinonovacula constricta and Solen gradis) classified as poly-saccharides, proteins and peptides, possess the antitumor, anti-aging, and anti-bacterial activities. Cancer patients undergoing long-term chemotherapy usually suffer from the damage to vital organs, and resistances affecting the outcomes of chemotherapy. Therefore, it is imperative to search for anti-cancer drugs with low toxicity and high efficiency. At present, several marine mollusks are being studied. Summary on the antitumor activities of the marine mollusks extrats is presented, which might provide a basis for the development of marine anti-cancer drugs.

Objective To investigate the effect of family nursing intervention on infant brain injury rehabili-tation therapy. Methods 102 cases of brain injury infants were randomly divided into observation group(32 cases),intervention group(38 cases) and control group (32 eases), respectively. The patients in observation group received routine treatment, while those in intervention group received family nursing intervention based on routine treatment.The children in control group whose parents refused the comprehensive rehabilitation therapy. All subjects were as-sessed by Gesell test before and after rehabilitation treatment. Results Compared with the control group patients, all indicators in the observation group and the intervention group were significantly improved. There were also signifi-cant differences between observation group and the intervention group( P < 0.01 ). Conclusion Family nursing in-tervention can significantly improve the effect on infant brain injury rehabilitation therapy.

Objective To observe the clinical effect of gonadotropin releasing hormone analogues in treatment of the girls with idiopathic central precocious puberty(ICPP). Methods Twelve girls with ICPP received therapy with GnRH-A.Before and after the end of treatment for 6 months,secondary sexual characteristics and growth velocity were observed,pelvic ultrasonic examination,X-ray bone age(BA),serum level of E_2 and LHRH stimulating test were detected,and the predicted adult height(PAH) was analysed. Results After treatment,the size of breasts,uterus and ovaries were significantly reduced.The basic and peak levels of serum LH and FSH by LHRH stimulation were also significantly reduced.The ratio of BA/CA(chronology age)was decreased.The PAH was increased. Conclusions GnRH-A can effectively depress the sexual characteristics,reduce the maturation of BA,and also improve the PAH in girls with ICPP.

In this studies, an anticancer peptide was isolated from the enzymatic hydrolysate of Mytilus coruscus.CCK-8 assay was used as guide in activity screening through the isolation process of membrane filtration, DEAEsepharose chromatography, Sephadex G-25 exclusive chromatography and RP-HPLC. An anticancer peptide was obtained and identified as Asp-Leu-Tyr.

Hyperphosphatemia is one of the common complications in maintenance hemodialysis patients and is closely related to cardiovascular disease and related death events. Therefore, the effective management of blood phosphorus is an important link to improve the prognosis of patients with maintenance hemodialysis. This study on maintenance hemodialysis patients with hyperphosphatemia 3Ds management including diet, along with the progress of dialysis and drug related nursing intervention were summarized, discuss how to reasonable dietary phosphorus limited, improve the efficiency of dialysis, and the correct use of problems still existing in phosphate binder, in order to reduce hyperphosphatemia to provide the reference for clinical nursing practice.

Objective To discuss the coping methods of pediatric nurses with adverse psychology and behavior of parents with terminal children. Methods 198 parents who were assessed with adverse psychology and behavior with terminal children were chosen and randomly divided into the observation group(100 cases) and the control group(98 cases). The control group was given conventional care, while in the observation group, nursing intervention of humanistic care and reinforced health education were adopted in addition. The changes of adverse psychology and behavior of the two groups were observed. Χ2 test was used. Results The adverse psychology and behavior in the observation group was reduced significantly than that of the control group after nursing intervention. Conclusions Nursing intervention can effec-tively mitigate the adverse psychology and behavior in parents with terminal children. It plays an active role beth on the terminal children and their parents.

Objective: To compare the efficacy and side effects of patient-controlled intravenous morphine with epidural single bolus morphine in postoperative pain relief. Method: Sixty patients undergoing gynecological procedures under epidural anesthesia were randomly assigned to epidural morphine(EPI)group or patient-controlled intravenous analgesia (PCIA) group. In the EPI group,2 mg of morphine was injected into epidural space at the end of operation. In PCIA group, 1 mg of morphine as a demand dose would be injected intravenously by the patient through a patientcontrolled analgesic delivery system until the pain relieved. The patients were followed up at 4, 8, 12, 24 h after operation,and the degree of pain,sedation, nausea and vomiting were assessed. Result: The total dosage of morphine was higher in the PCIA group(19.08?5.0 mg)than that in the EPI group(2mg,P

Objective To discuss the diagnostic accuracy of 2B-mode feature model in grading the degree of hepatic fibrosis compared to acoustic radiation force impulse (ARFI) and liver biopsy.Methods A total of 140 patients was enrolled in the study and divided into four groups (F1-F4) according to pathological grading using METAVIR scores system,and 47 healthy volunteers were enrolled as the control group (F0) at random.All of subjects underwent standard ultrasound examination and acoustic radiation force impulse (ARFI).Ultrasound raw images were obtained and analyzed with 2B-mode feature intelligent model and then compared to the value of liver stiffness (ARFI).Results The area under the receiver operating characteristic (ROC) curve of grading of liver fibrosis (F2) using 2B-mode feature intelligent model was training =0.973 2,and testing =0.751 1,which was superior to the area under the ROC curve (F2) using ARFI with training =0.840 1,and testing =0.656 4.Conclusions 2B-mode feature intelligent model could be used for grading of liver fibrosis in patients with chronic hepatitis B (CHB).There is great potential in the quantitative diagnosis of liver fibrosis stage using 2B-mode ultrasound.

{L-End}Objective To analyze sound pressure level and spectrum characteristics of noise from various equipment in noise-related worksites in wooden furniture enterprises in Zhongshan City. {L-End}Methods A total of 107 wooden furniture enterprises with equipment sound pressure levels ≥85.0 dB(A) were selected as the research subjects using the judgment sampling method. Individual noise meters were used to detect the sound level and spectrum of equipment in noise-related worksites. Cluster analysis was used to analyze the noise spectrum characteristics of each noise equipment. {L-End}Results The incidence of sound pressure level exceeding the national standard of occupational exposure limits for noise in workplace among the research subjects was 60.4% (365/604). The equipment with the highest sound pressure level was the sliding table saw (rough shape cutting position) at 101.4 dB(A), while the lowest was the sanding machine (grinding position) at 85.0 dB(A). The clustering analysis results showed that the noise spectrum characteristics of nail guns, sliding table saw, edge banders, spray guns, and drilling machines were similar, with the highest sound pressure level of 4 000.0 Hz, mainly concentrating in the high-frequency. The noise spectrum characteristics of punching machines, sanding machine, lathes, and engraving machines were similar, with the highest sound pressure level ranging from 500.0 to 1 000.0 Hz, mainly concentrating in the mid-frequency. The noise spectrum characteristics of drilling machines, planers, hot press machines, and mortise and tenon machines were similar, with the highest sound pressure level ranging from 500.0 to 1 000.0 Hz, mainly concentrating in the mid- and high-frequency. The sound pressure level of the mixer reached its peak at 8 000.0 Hz, demonstrating a wide frequency domain characteristic of sound pressure level with the presence of low-, mid- and high-frequencies. {L-End}Conclusion The noise-exposed hazards of wooden furniture enterprises are relatively serious, and the spectral characteristics of the equipment with noise are distinct. It is recommended to implement targeted noise reduction measures and personal hearing protection based on the specific spectrum characteristics of equipment noise.