Objective To test the reliability and validity of the Chinese version of the Pregnancy Exercise Self-Efficacy Scale(P-ESES) among Chinese population. Methods The Chinese version of P-ESES was translated from the original one,back-translated and adjusted for cultural adaptation. The reliability and validity were tested among 274 eligible pregnant women recruited in one obstetric outpatient clinic from a tertiary hospital in Guangzhou using convenience sampling. Results The content validity index (CVI) of the Chinese version of P-ESES was 0.98. Factor analysis extracted three common factors,which explained 60.649% of the total variance,and each item had high fac-tor loading quantity(>0.4). The test-retest reliability was 0.531,and Cronbach's α coefficient was 0.804. Conclusion The Chinese version of the P-ESES has been proved to be reliable and valid. It is a valuable tool for evaluating exercise self-efficacy among pregnant women in mainland China.

Objective To investigate the relationships between exercise and exercise self-efficacy and knowledge in pregnant women. Methods A total of 274 pregnant women were recruited from a tertiary level first class hospital from July to November 2015 in Guangzhou by convenience sampling method. They were investigated with the social relational scale, the Pregnancy Physical Activity Questionaire, the Pregnancy Exercise Self-efficacy Scale, the Pregnancy Exercise knowledge Scale. Results Only 22.6％ (62/274) of the women met the international guideline for exercise. Score of the exercise self-efficacy and exercise were positively correlated(r=0.203, P=0.000). Score of the knowledge and exercise were positively correlated(r=0.103, P=0.032). Score of the exercise self-efficacy and knowledge were meaningless(r=0.098, P=0.106). Conclusions It is suggested to take more actions to enhance the recognition of pregnant women for exercise. Conviction formed after thinking knowledge gradually, by faith again dominate action, ultimately promote the perinatal health of pregnant women.

Objective To investigate the effect of family nursing intervention on infant brain injury rehabili-tation therapy. Methods 102 cases of brain injury infants were randomly divided into observation group(32 cases),intervention group(38 cases) and control group (32 eases), respectively. The patients in observation group received routine treatment, while those in intervention group received family nursing intervention based on routine treatment.The children in control group whose parents refused the comprehensive rehabilitation therapy. All subjects were as-sessed by Gesell test before and after rehabilitation treatment. Results Compared with the control group patients, all indicators in the observation group and the intervention group were significantly improved. There were also signifi-cant differences between observation group and the intervention group( P < 0.01 ). Conclusion Family nursing in-tervention can significantly improve the effect on infant brain injury rehabilitation therapy.

Objective To detect the expression of HBV in human early embryos of infertility patients combined with hepatitis B virus (HBV) infection after undergoing in vitro fertilization and embryo transfer (IVF-ET) treatment,so as to provide reliable evidence for the vertical transmission of HBV through human germ cells.Methods We collected the abandoned early embryos of patients combined with HBV infection after undergoing IVF-ET treatment in the department of reproductive medicine of the First People's Hospital of Yunnan Province,total genomic DNA was obtained by single cell PCR,and then polymerase chain reaction (PCR) was used to detect the expression of HBV-DNA and covalently closed circular DNA of hepatitis B virus (HBV-cccDNA) in early embryos.Results Both HBV-DNA and HBV-cccDNA were detected in the early embryo among the mother or father or parents HBV infected patients.The positive detection rate of HBV-DNA in mother,father and parents HBV infected patients was 78.9％,76.3％ and 100％,and the positive rate of HBV-cccDNA was 63.2％,78.9％ and 100％.The difference between the mother and the father was not significant (P＞O.05).However,the positive rate in parents HBV infected patients group was significantly higher than the mother or father group,showing a significant difference (P＜0.05).Conclusion HBV can be transmitted from mother or father to early embryos via IVF-ET treament,and the risk of vertical transmission increases significantly when the couple are HBV infected.

Objective To discuss the coping methods of pediatric nurses with adverse psychology and behavior of parents with terminal children. Methods 198 parents who were assessed with adverse psychology and behavior with terminal children were chosen and randomly divided into the observation group(100 cases) and the control group(98 cases). The control group was given conventional care, while in the observation group, nursing intervention of humanistic care and reinforced health education were adopted in addition. The changes of adverse psychology and behavior of the two groups were observed. Χ2 test was used. Results The adverse psychology and behavior in the observation group was reduced significantly than that of the control group after nursing intervention. Conclusions Nursing intervention can effec-tively mitigate the adverse psychology and behavior in parents with terminal children. It plays an active role beth on the terminal children and their parents.

Objective To investigate the correlation between asymmetric dimethylarginine (AD-MA) and endothelial dysfunction in patients with uremia.Methods Uremic patients who did not receive hemodialysis were defined as A group (n =40) ; uremic patients who had received hemodialysis were divided into B group (n =45) ;healthy people were defined as C group (n =20) ;and chronic kidney disease (stage 2 ～ 4) patients were defined as D group (n =20).The diameter of intima-media thickness,and endothelium-dependent or independent dilation (EDD or EID) of radial artery in right forearm were detected with diasonography.The levels of ADMA were determined by high-performance liquid chromatography.Results Compared to C group,the levels of ADMA in A,B and D groups were significantly increased [C:(0.78 ±0.19) μmol/L,A:(1.51 ±0.16) μ mol/L,B:(1.13 ±0.14) μmol/L,D:(0.92 ±0.11) μmol/L; P ＜0.05].Compared to A group,the levels of ADMA were significantly decreased in B group (P ＜0.05).EDD and EID were decreased significantly in A,B and D groups compared to C group [EDD:C:(13.52±1.73)％ vs A:(7.32 ±0.54)％,B:(9.02 ±0.86)％,D:(10.13 ±1.25)％,P ＜0.05;EID:C:(14.45±1.85)％ vsA:(10.37 ±1.51)％,B:(9.54±1.39)％,D:(11.17±1.56)％,P ＜0.05].EDD in B group was significantly lower than A group (P ＜0.05).In group A,a negative correlation was found between EDD and the level of ADMA (r =-0.81,P =0.020).Conclusions ADMA level was significantly increased in uremic patients.A close correlation existed between ADMA and endothelial dysfunction of radial artery.

Objective To explore the protection of valsartan combined with simvastatin on kidney in early diabetic nephropathy rats. Methods Diabetic nephropathy rats model were induced by streptozocin (STZ) ,the experimental rats were randomly divided into 5 groups: control (group C), diabetic nephropathy (group D) ,diabetes treated with valsartan (group X) ,diabetes treated with simvastatin (group Z) ,and diabetes treated with combined valsartan and simvastatin ( group L). Blood glucose (BG), HbA1c, blood cholesterol ( TC), trigalloylglycerol ( TG ), blood ureanitrogen ( BUN ), serum creatinine (SCr) , urinary albumin excretion rate (UAER) were measured, and the podocyte ultrastructure was observed by transmission electronic microscopy. Results The levels of BG, HbA1c,TC,TG and UAER in group D increased significantly compared togroup C(BG:[20.3 ±3.2]mmol/L vs [6.1 -±0. 4]mmol/L;HbA1c:[7.18 ±0.47]% vs [3.37 ±0. 15]% ;TC: [2. 69 ±0. 35] mmol/L vs [1.28 ±0. 24] mmol/L;TG: [3.09 ±0. 37] mmol/L vs [1.18 ±0. 25]mmol/L) (P ＜ 0. 05 ). Creatinine clearance rates (Ccr) in group D ( [0. 89 ± 0. 19] ml/min ) decreased significantly compared to group C( [1.27 ±0. 33] ml/min) ,as well as group X,Z and L( Ps ＜ 0. 05 ). UAER in group D was significantly higher than that in group C ( [19. 87 ±3. 85] μg/24 h vs [3. 67 ± 1.01] μg/24 h) (P ＜ 0. 05 ), as well as group X, Z and L ( P ＜ 0. 05 ), and the improvement in group L was particularly significant ( P ＜ 0. 05 ). The projections of podocyte in group D severely syncretized, there were slightly improvement in group X, Z and L compared to group D, and the improvement in group L was remarkable. Conclusion The treatment with valsartan, simvastatin and their combination will effectively protect the kidney in early diabetic nephropathy rats,and the effect of using the combination therapy is much better.

Objective To study the effects of efflux pump inhibitors(CCCP and PAβN)on carbapenems in Pseudomonas aernginosa(P.aeruginosa)clinical isolates and investigate the association between the resistance to imipenem or meropenem and expression levels of efflux pumps of P.aeruginosa.Methods MICs of imipenem or meropenem combined with efflux pump inhibitors including carbonyl cyanide m-chlorophenylhydrazone(CCCP,107 strains)and Phe-Arg-β-naphthylamide(PAβN,71 strains)against imipenem-resistant strains were determined by agar dilution method,and changes of MICs were observed.For 32 strains with different resistant phenotypes to imipenem and meropenem,the mRNA expression levels of three efflux pump genes(mexA,mexD and mexF)were quantified by real time fluorescent quantitative PCR.Results The resistance rate of imipenem and meropenem didn't prove any significant difference in the presence of efflux pump inhibitors.The X2 value of imipenem combined with CCCP and PAβN were 0.338 and 0.086,respectively(P＞0.05),while that of meropenem combined with CCCP and PAβN were 1.065 and 1.458(P＞0.05).No significant in MICs of carbapenems were seen in over half of P. aeruginesa isolates. MICs of carbapenems was significantly downregulated for 4-fold or above in eight isolates. Overexpression of efflux pumps genes were present in 24 of 27 carbapenem-resistant isolates(88. 9% ). Efflux pumps genes including MexAB-OprM, MexCD-OprJ and MexEF-OprN were all overexpressed in 13 isolates,constituting 54. 2% of all carbapenem-resistant isolates. There were 3 isolates in which beth MexAB-OprM and MexCD-OprJ showed overexpression,constituting 12. 5%. Also,MexAB-OprM and MexEF-OprN overexpressed in 3 isolates. There were 2 isolates (8.3%) showing MexEF-OprN overexpression and MexAB-OprM alone. MexCD-OprJ didn't showed overexpression alone. Furthermore,the expression levels of efflux pumps genes mexA,mexD and mexF in isolates susceptible to both in imipenem and meropenem were 0. 48±0. 48,0. 48±0. 53 and 0. 30±0. 41,respectively,which were much lower than that in carbapenem-resistant ones (P＜0. 05 ). MexA gene was expressed at a higher level in meropenemresistant isolates than meropenem-susceptible ones (P＜0. 05 ). Conclusions When the concentration of CCCP and PAβN were 5 μg/ml and 20 μg/ml respectively,the efforts on the carhapenems resistance of P.aeruginosa were small Overexpression of MexAB-OprM might play an important role in meropenemresistance in P. aerugines. Overexpression of MexCD-OprJ and MexEF-OprN was associated with imipenemresistance. However,the relationship between them and meropenem-resistance need to be explored in the future.

OBJECTIVE: To analyze the principle mechanism of the arcus plantaris and its clinical application. METHODS: The states of forces sustained by the arcus plantaris were analyzed and calculated according to the mechanism of the quadratic parabolic arch. RESULTS: The aponeurosis plantaris corresponded to the pull rod of the arcus plantaris. The medial and lateral longitudinal arches formed by the pedal bones were stable with the rod, but unstable without the rod. In the latter condition, on loading, the force sustained by the parabolic arch became a force sustained by a simple beam, and the arcus plantaris tended to disappear and to be flattened. Clinically, 240 feet with talipes equinus were treated with triple arthrodesis. In 34 out of the reexamined 156 feet, the aponeurosis plantaris was cut in addition to the triple arthrodesis and was immobilized with cast for 3 months. One or two years later, their arcus plantaris disappeared, pain developed when walking, and some of them walked with the midtarsal joint against the ground. Then, the triple arthrodesis and shortening of the aponeurosis plantaris were applied on 18 cases, and osteotomy of the calcaneus and reconstruction of the aponeurosis plantaris were made on 10 cases and satisfactory effects were obtained. CONCLUSIONS: In order to achieve satisfactory therapeutic effects of the triple arthrodesis, we should reestablish the arcus plantaris and accurately treat the aponeurosis plantaris for the balance of the surrounding muscle force.

Objective To explore the effect of virtual reality(VR)technology on relieving intraoperative pain in patients receiving transcatheter hepatic artery chemoembolization(TACE).Methods A total of 76 patients,who received TACE from June 2023 to January 2024,were enrolled in this study.Using random number table method,the patients were divided into control group(n=38)and study group(n=38).Intraoperative routine nursing was carried out for the patients of the control group,while on the basis of routine nursing additional VR technology was adopted to relieve the intraoperative pain for the patients of the study group.The degree of intraoperative pain,anxiety symptoms,incidence of intraoperative adverse reactions and patient satisfaction with nursing were compared between the two groups.Results The degree of intraoperative pain in the study group was lower than that in the control group,but the difference between the two groups was not statistically significant(P＞0.05).The analgesic effect of VR was much obvious in patients aged≤55 years and in patients with vascular invasion of liver cancer(P＜0.05).The anxiety score in the study group was lower than that in the control group,the patient satisfaction score in the study group was higher than that in the control group,and the differences between the two groups were statistically significant(both P＜0.05).No statistically significant difference in the incidence of adverse reactions existed between the two groups(P＞0.05).Conclusion Immersive VR technology can effectively reduce the degree of intraoperative pain in patients receiving TACE,especially in patients aged ≤55 years.Besides,VR technology can also reduce the anxiety degree of patients,and improve the degree of intraoperative patient satisfaction with nursing.