1.Advances in the study of antitumor activities of the marine mollusks extracts
Fangfang HUANG ; Yongfang YANG ; Guofang DING
Journal of China Pharmaceutical University 2009;40(3):284-288
The extracts of marine mollusks( Abalone, Aplysia, Sepia, Arcagranosa linnaeus, Cyclina sinensis, Myti-lus coruscus, Chlamys farrei, Meretrix meretrix, Ostrea, Sinonovacula constricta and Solen gradis) classified as poly-saccharides, proteins and peptides, possess the antitumor, anti-aging, and anti-bacterial activities. Cancer patients undergoing long-term chemotherapy usually suffer from the damage to vital organs, and resistances affecting the outcomes of chemotherapy. Therefore, it is imperative to search for anti-cancer drugs with low toxicity and high efficiency. At present, several marine mollusks are being studied. Summary on the antitumor activities of the marine mollusks extrats is presented, which might provide a basis for the development of marine anti-cancer drugs.
2.Effect of Xiaoerkechuanning Granule on Allergic Asthma in Guinea Pigs
Yongfang DING ; Yunru PENG ; Mingqin SHEN
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(05):-
Objective To study the antiasthmatic effect of Xiaoerkechuanning Granule (KCN) in guinea pigs with allergic asthma. Method The allergic asthma model in guinea pigs was induced by ovalbumin (OVA), and detemine the incubation period of asthma, the content of malondiadehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) in blood serum, histopathologic changes of lungs. Results The KCN can markedly prolong the incubatin period, increase the activities of SOD, alleviate the histopathologic changes. Conclusion KCN has obviously protective effects on experimental allergic asthma in guinea pigs.
3.Antiproliferation of human colon carcinoma cell line(SW480)by Compound Changtai Granule
Shu ZHANG ; Mingqin SHEN ; Yongfang DING ; Xiaoning WANG
Chinese Traditional Patent Medicine 2010;(3):374-378
AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG)on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could successfully prevent SW480 cell from entering G_0/G_1 phase and G_2/M phase,decrease cell numbers in S phase at the same time.CONCLUSION:CCG has an obvious dose-response relationship in the range of 3.51 to 7.81 mg/mL.
4.Isolation and purification of an anticancer activity peptide from protein hydrolysate of Mytilus coruscus
Yongfang YANG ; Haiqiang YAN ; Guofang DING ; Fangfang HUANG
Journal of China Pharmaceutical University 2011;42(3):272-275
In this studies, an anticancer peptide was isolated from the enzymatic hydrolysate of Mytilus coruscus.CCK-8 assay was used as guide in activity screening through the isolation process of membrane filtration, DEAEsepharose chromatography, Sephadex G-25 exclusive chromatography and RP-HPLC. An anticancer peptide was obtained and identified as Asp-Leu-Tyr.
5.Antiproliferation of human colon carcinoma cell line (SW480) by Compound Changtai Granule
Shu ZHANG ; Mingqin SHEN ; Yongfang DING ; Xiaoning WANG
Chinese Traditional Patent Medicine 1992;0(03):-
AIM:To check the antiproliferative and apoptosis-inducing effect of Compound Changtai Granule(Radix et Rhizoma ginseng,Semen coicis,Rhizoma curcumae,etc)(CCG) on human colon carcinoma cell line(SW480).METHODS:Methy thiazolyl tetrazolium chromatomerry was used to observe the influence of several concentration of CCG on SW480 cell proliferation cultured in vitro at the various times,and flow cytometry was applied to examining the change in SW480 cell proliferation cycle and apoptosis rate.RESULTS:CCG could successfully prevent SW480 cell from entering G0/G1 phase and G2/M phase,decrease cell numbers in S phase at the same time.CONCLUSION:CCG has an obvious dose-response relationship in the range of 3.51 to 7.81 mg/mL.
6.Comparison of clinical effects of a novel stent assisted intestinal bypass and temporary loop ileostomy in laparoscopic low anterior resection of rectal cancer
Liushiyang XU ; Shiyu HU ; Wanbo HUANG ; Jianming XIE ; Jiabin YANG ; Yongfang YIN ; Haixiang DING ; Zhilong YAN
Chinese Journal of General Surgery 2023;38(6):401-406
Objective:To compare the clinical value of stent assisted intestinal bypass and temporary loop ileostomy in laparoscopic low anterior resection of rectal cancer.Method:In this retrospective analysis, 57 patients undergoing laparoscopic low anterior resection for rectal cancer in the First Affiliated Hospital of Ningbo University from Jan 2020 to Jan 2022 were divided into intestinal bypass group (36 cases) and loop ileostomy group (21 cases).Result:There were no significant differences in postoperative GI function recovery and postoperative complication rate between the two groups (all P>0.05). The levels of albumin, prealbumin and hemoglobin in the intestinal bypass group were better than those in the ileostomy group when evaluated on 3rd months after operation [(40.5±2.3) g/L vs. (38.1±2.6)g/L、(26.4±2.7)mg/dl vs. (24.5±2.0)mg/dl、(137.6±5.9) g/L vs. (134.0±7.0) g/L, t=3.605、2.743、2.085, all P<0.05]. Hospital expenses of the intestinal bypass group was lower [(571 000±7 500) yuan vs. (69 300±9 100) yuan, t=-5.477, P<0.05]. Conclusion:Compared with traditional ileostomy, the stent assisted intestinal bypass reduces trauma with lower expenses and improves patients' status after laparoscopic low anterior resection for rectal cancer.