Objective To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.Methods Twenty-eight SD male young rats ( 4 weeks old ) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ±15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight ( bw) every day for 30 days.The bone marrow cells were collected for micronuclei and γ-H2 AX immunofluorescence analysis.Results The percentage of γ-H2 AX foci-positive cells (37.4 ±10.0)%in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ±3.1)% (t value was -17.59, P<0.01).No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte ( PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.Conclusion TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells , but has no effect on micronucleus of bone marrow cells in young rats.

Objective To explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.Methods Twenty-eight SD male young rats ( 4 weeks old ) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ±15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight ( bw) every day for 30 days.The bone marrow cells were collected for micronuclei and γ-H2 AX immunofluorescence analysis.Results The percentage of γ-H2 AX foci-positive cells (37.4 ±10.0)%in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ±3.1)% (t value was -17.59, P<0.01).No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte ( PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.Conclusion TiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells , but has no effect on micronucleus of bone marrow cells in young rats.

OBJECTIVETo explore the genotoxic effects of oral-exposed TiO2 nanoparticles on bone marrow cells in young rats.

METHODSTwenty-eight SD male young rats (4 weeks old) were randomly divided into 4 groups, which were exposed to TiO2 nanoparticles ((75 ± 15) nm, anatase) through intragastric administration at 0, 10, 50 and 200 mg/kg body weight (bw) every day for 30 days. The bone marrow cells were collected for micronuclei and γ-H2AX immunofluorescence analysis.

RESULTSThe percentage of γ-H2AX foci-positive cells (37.4 ± 10.0)% in the 50 mg/kg bw dose group were significantly higher than that in the control group (19.8 ± 3.1)% (t value was -17.59, P < 0.01). No significantly difference was found in polychromatic erythrocyte/normochromatic erythrocyte (PCE/NCE) ratio and PCE micronucleus rate between three experimental groups and control group.

CONCLUSIONTiO2 nanoparticles can increase the frequency of DNA double-strand breaks in bone marrow cells, but has no effect on micronucleus of bone marrow cells in young rats.

Animals ; Bone Marrow Cells ; DNA Damage ; Histones ; Male ; Micronuclei, Chromosome-Defective ; Nanoparticles ; Rats ; Titanium