1.Correction: Methods of Hematoxylin and Eosin Image Information Acquisition and Optimization in Confocal Microscopy.
Woong Bae YOON ; Hyunjin KIM ; Kwang Gi KIM ; Yongdoo CHOI ; Hee Jin CHANG ; Dae Kyung SOHN
Healthcare Informatics Research 2016;22(4):355-355
In the article, Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy, there was a typographical error in the title.
2.Methods of Hematoxylin and Erosin Image Information Acquisition and Optimization in Confocal Microscopy.
Woong Bae YOON ; Hyunjin KIM ; Kwang Gi KIM ; Yongdoo CHOI ; Hee Jin CHANG ; Dae Kyung SOHN
Healthcare Informatics Research 2016;22(3):238-242
OBJECTIVES: We produced hematoxylin and eosin (H&E) staining-like color images by using confocal laser scanning microscopy (CLSM), which can obtain the same or more information in comparison to conventional tissue staining. METHODS: We improved images by using several image converting techniques, including morphological methods, color space conversion methods, and segmentation methods. RESULTS: An image obtained after image processing showed coloring very similar to that in images produced by H&E staining, and it is advantageous to conduct analysis through fluorescent dye imaging and microscopy rather than analysis based on single microscopic imaging. CONCLUSIONS: The colors used in CLSM are different from those seen in H&E staining, which is the method most widely used for pathologic diagnosis and is familiar to pathologists. Computer technology can facilitate the conversion of images by CLSM to be very similar to H&E staining images. We believe that the technique used in this study has great potential for application in clinical tissue analysis.
Diagnosis
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Eosine Yellowish-(YS)
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Fluorescence
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Hematoxylin*
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Image Processing, Computer-Assisted
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Methods*
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Microscopy
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Microscopy, Confocal*
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Staining and Labeling