ObjectiveTo study the influence of Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction) on signal pathway of c-jun N-terminal kinase (JNK) and inflammatory factors in mice with diabetic peripheral neuropathy (db/db mice),and discuss its mechanism in protecting peripheral nerve.Methods The db/db mice (n =40) were randomly divided into 5 groups:model group,lipoic acid group,high-dose Yiqi Huoxue Tongluo Fang group (6.4 g/kg,high-dose group),mid-dose Yiqi Huoxue Tongluo Fang group (3.2 g/kg,mid-dose group) and low-dose Yiqi Huoxue Tongluo Fang group (1.6 g/kg,low-dose group),and other db/m mice (n =8) were chosen into normal group.All groups were intragastrically given corresponding drugs once a day for 8 weeks.After the last administration of drugs,the plantar thermal response time and motor nerve conduction velocity (MNCV) were detected.The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by using enzyme-linked immunosorbent assay (ELISA).The protein expressions of JNK and phosphorylase JNK (p-JNK) in dorsal root ganglion (DRG) were detected by using Western blot method.Results Compared with normal group,MNCV was slower,plantar thermal response time was longer,levels of IL-1β and TNF-α increased,and protein expressions of JNK and pJNK are stronger in model group (P ＜ 0.01 or P ＜ 0.05).Compared with model group,Yiqi Huoxue Tongluo Fang promoted MNCV,shortened plantar thermal response time,decreased levels of IL-1 β and TNF-α and down-regulated protein expressions of JNK and p-JNK to some extent (P ＜ 0.01 or P ＜0.05).ConclusionYiqi Huoxue Tongluo Fang can inhibit the abnormal activation of JNK signal pathway and relieve inflammatory reaction to protect peripheral nerve in db/db mice.

OBJECTIVETo research the central molecular mechanism of gastric motility in functional dyspepsia (FD) rats treated with electroacupuncture (EA) at and points of stomach.

METHODSA total of 30 SD rats were randomized into a blank group, a model group, a Zhongwan+Weishu group, a Weishu group and a Zhongwan group, 6 rats in each group. FD rats were established by moderate clipping tail infuriation and irregular feeding except in the blank group. EA was used at "Zhongwan"(CV 12),"Weishu"(BL 21), and"Zhongwan"(CV 12) +"Weishu"(BL 21) in the corresponding groups for 7 days, once a day, and 20 min a time. No intervention was used in the blank and model groups. Grabbing and fixation were applied in the model group. Gastric antrum motion range and frequency were recorded by gastrointestinal pressure transducer. The expression of subunit NR1 of N-methyl-D-aspartate recepter (NMDAR) in dorsal motor nucleus of the vagus (DMV) was determined by Western blotting. The content of serum nitric oxide (NO) was measured by ELISA.

RESULTSCompared with the blank group, the gastric antrum motion range and NR1 in the DMV decreased and the serum NO content increased in the model group (all <0.05). Compared with the model group, the gastric antrum motion range and NR1 in the DMV increased and the serum NO content decreased in the three EA groups (all <0.05). Compared with the Zhongwan and Weishu groups, the gastric antrum motion range and NR1 in the DMV increased in the Zhongwan + Weishu group (all <0.05). Compared with Zhongwan + Weishu and Zhongwan groups, the expression of NO in the Weishu group decreased (both <0.05). The gastric antrum motion frequency among the 5 groups had no statistical significance (all >0.05).

CONCLUSIONEA at the and points can regulate the gastric motility in FD rats which may be by modulating the activity of NMDAR in the central DMV region, thus regulating the serum NO content.

Acupuncture Points ; Animals ; Dyspepsia ; therapy ; Electroacupuncture ; Gastrointestinal Motility ; N-Methylaspartate ; metabolism ; Nitric Oxide ; blood ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Stomach ; Vagus Nerve ; metabolism

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