Background: Porcine circovirus type 2 (PCV2) is an important infectious pathogen implicated in porcine circovirus-associated diseases (PCVAD), which has caused significant economic losses in the pig industry worldwide. Objectives: A suitable viral vector-mediated gene transfer platform for the expression of the capsid protein (Cap) is an attractive strategy. Methods: In the present study, a recombinant adeno-associated virus 8 (rAAV8) vector was constructed to encode Cap (Cap-rAAV) in vitro and in vitro after gene transfer. Results: The obtained results showed that Cap could be expressed in HEK293T cells and BABL/c mice. The results of lymphocytes proliferative, as well as immunoglobulin G (IgG) 2a and interferon-γ showed strong cellular immune responses induced by Cap-rAAV. The enzyme-linked immunosorbent assay titers obtained and the IgG1 and interleukin-4 levels showed that humoral immune responses were also induced by Cap-rAAV. Altogether, these results demonstrated that the rAAV8 vaccine Cap-rAAV can induce strong cellular and humoral immune responses, indicating a potential rAAV8 vaccine against PCV2. Conclusions The injection of rAAV8 encoding PCV2 Cap genes into muscle tissue can ensure long-term, continuous, and systemic expression.

Objective To investigate the cognition status and possible ways of patients′ privacy divulgence among medical staffs and patients, and to explore feasible strategies to protect the privacy. Methods A self-designed questionnaire about privacy cognition was used to investigate 92 patients and 59 medical staffs on one day in July 2014. Results The majority of patients had certain cognition of individual privacy;34. 8%, 30. 4% and 28. 3% of the patients thought the test outcomes, history of infection, illness conditions were private issues;63. 0%, 39. 1% and 31. 5% of the patients thought the perineum, pygal and chest were private parts. Medical staffs had significant higher privacy cognition compared with the patients ( P<0. 05);86. 4%, 86. 4% and 78. 0% of the staffs thought the emotional experiences, the history of infection, test outcomes were private issues;100. 0%, 89. 8% and 86. 4% of the staffs thought the perineum, pygal and chest were private parts. The private information of the patients would be divulged during the medical care such as:patient rounds and physical examinations, asking for the history of the illness, nursing, room layout, and talking between the medical staffs. Conclusions The medical staffs should enhance and motivate the concept of privacy among the patients in order to reduce the differences between these two groups and to improve the relationship between doctors and patients.