1.Expression and clinical significance of Pin1 in pancreatic carcinoma
Songzhu YANG ; Meiju LIN ; Yongbo WANG ; Heshui WU
Journal of Endocrine Surgery 2011;05(1):24-26
Objective To investigate the expression of Pin1 in human pancreatic carcinoma as well as adjacent tissues and to discuss the role of Pinl in oncogenesis of pancreatic carcinoma. Methods Specimen of pancreatic carcinoma tissues and adjacent tissues were collected from 20 cases. Pin1 mRNA and protein expression in pancreatic neoplasm and corresponding adjacent nontumorous tissues were detected by real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR) and western blot. Results Pin1 was overexpressed at mRNA and protein level in pancreatic carcinoma tissues compared with that in their nontumorous counterparts ( 2.78 ± 1.02 vs 4.36 ± 1.27;5. 48 ± 1.69 vs 9.97 ± 1.86, P < 0.05 ). Pin1 expression was not correlated to clinical stage and pathological grading of the carcinoma. Conclusion Pin1 overexpression may play a key role in pancreatic carcinoma.
2.Study on Status of Nosocomial Transmission of Mycobacterium Tuberculosis and Measures for Prevention and Control
Fang WANG ; Bin YI ; Jun LIU ; Lin LIU ; Yongbo CHENG ; Xianchun TU
Chinese Journal of Nosocomiology 2009;0(21):-
OBJECTIVE To discuss the prevention and control measures for nosocomial transmission of Mycobacterium tuberculosis.METHODS The status of hospital-acquired tuberculosis and influencing factors on nosocomial transmission of M.tuberculosis at home and abroad were analyzed.Then prevention and control measures for nosocomial tuberculosis were brought forward.RESULTS To prevent and control of nosocomial transmission of M.tuberculosis,it was necessary to strengthen personnel management and environmental monitoring,control the direction of air flow in hospitals,strengthen respiratory protection of health care workers and management of tuberculosis patients.CONCLUSIONS To prevent and control M.tuberculosis hospital infections,comprehensive prevention and control measures are needed.
3.Stress distribution in natural maxillary central incisor and implant: a three-dimensional finite element analysis
Linzi WAN ; Lei SUN ; Xifeng WU ; Zhenyan LIN ; Liangwei XU ; Zhenzhe LIU ; Duoling XU ; Yang LI ; Yanmin ZHOU ; Yongbo GAO
Chinese Journal of Tissue Engineering Research 2015;19(16):2545-2550
BACKGROUND:Biomechanical compatibility is the necessary condition to ensure the stable osseointegration with implants that then can function over a long period; therefore, it is especialy important to get knowledge about distribution of stress and strain between the maxilary central incisor and its surrounding bone tissue. OBJECTIVE: Based on five different anatomical types of natural teeth, to study the regularity of stress distribution between the maxilary central incisor root and implant.METHODS: According to the five different anatomical types of natural maxilary central incisors, UGNX and ANSYS were used to set up three-dimensional finite element models (B1, B2, M1, M2, P1) for the implant and surrounding structures, which were under 100 N static load at angles of 0o, 30o, 45o, 60o, 90o with the long axis of teeth. Then, the stress distribution between the five kinds of maxilary central incisor roots and implants was analyzed. RESULTS AND CONCLUSION:Among the five different anatomical types, the equivalent stress for both the natural central incisor and implant were increased with the increasing of angles, and the implant had a higher raising trend. The equivalent stress for the natural tooth concentrated upon B1 for the maximum value and M1 for the minimum value; while the equivalent stress for the implant focused on the maximum value at M1 and the minimum value at M2. There was a gap of 2%-31% between the equivalent stresses for the natural tooth roots and a gap of 4%-21% for the implants. The stress distribution range for the implant was just smaler than that for the natural tooth roots. It implies that the bit force of implant and natural tooth is in positive proportion to the bite angles, and the bite force that implant can burden is smaler than that the central incisor can.
4.Effects of Shenmai injection combined with enteral nutrition on immune function of patients with severe cardiac insufficiency
Ke CUI ; Guoliang YU ; Yuanhuai ZHANG ; Yongbo JIANG ; Ronghai LIN ; Sheng ZHANG ; Lingzhu QIAN ; Yiping ZHOU
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2019;26(4):445-447
Objective To observe the effect of Shenmai injection combined with enteral nutrition (EN) on immune function in patients with severe cardiac insufficiency. Methods Fifty-seven patients with severe cardiac insufficiency admitted to the Department of Critical Care Medicine of Taizhou Hospital of Zhejiang Province from June 2015 to June 2018 were divided into an EN group (31 cases) and an EN group combined with Shenmai injection group (26 cases). The EN group was given EN on the basis of routine western medicine treatment, while in the EN combined with Shenmai injection group was treated additionally by intravenous drip of Shenmai injection 100 mL/d on the basis of above EN group treatment. The efficacies of the two groups were evaluated after consecutive 7-day treatment in the two groups. The changes in levels of subsets of T-lymphocytes (CD3+, CD4+, CD8+, CD4+/CD8+) and immunosuppressive cells CD14+ monocyte human leukocyte antigen DR (HLA-DR) were observed before and after treatment. Results After treatment, the levels of T-cell subsets CD3+, CD4+, CD4+/CD8+ and CD14+ monocytes HLA-DR in the peripheral blood of the two groups were significantly higher than those before treatment [CD3+: EN group was 0.539±0.126 vs. 0.379±0.093,Shenmai injection group was 0.652±0.185 vs. 0.393±0.091; CD4+: EN group was 0.402±0.121 vs. 0.275±0.066,Shenmai injection group was 0.524±0.168 vs. 0.281±0.077; CD4+/CD8+:EN group was 1.83±0.70 vs. 1.11±0.70,Shenmai injection group was 2.81±0.91 vs. 1.19±0.58; CD14+HLA-DR:EN group was (43.3±7.1)% vs. (35.4±5.7)%,Shenmai injection group was (54.9±6.2)% vs. (36.1±8.3)%]; After treatment, CD8+ in EN group decreased (0.223±0.052 vs. 0.253±0.081), while CD8+ in shenmai injection group increased (0.288±0.051 vs. 0.259±0.078), and the increase degrees of the above-mentioned indexes in EN combined with Shenmai injection group were more obvious than those in the EN group after treatment [CD3+: 0.652±0.185 vs. 0.539±0.126, CD4+: 0.524±0.168 vs. 0.402±0.121, CD8+: 0.288±0.051 vs. 0.223±0.052, CD4+/CD8+: 2.81±0.91 vs. 1.83±0.70, CD14+HLA-DR: (54.9±6.2)%, (43.3±7.1)%, all P < 0.05]. Conclusion The combined use of Shenmai injection and early EN can improve the immune function of T-lymphocytes in patients with severe cardiac insufficiency. The mechanism may be related to the enhancement of the activation of T lymphocytes and promotion of the CD14+ monocytes increase and immune function.
5.Synergistic Effect of Bone Marrow-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma in Streptozotocin-Induced Diabetic Rats.
Zhenzhen LIAN ; Xiaojing YIN ; Hua LI ; Lili JIA ; Xiuzhen HE ; Yongbo YAN ; Naihua LIU ; Kayiu WAN ; Xiaokun LI ; Shaoqiang LIN
Annals of Dermatology 2014;26(1):1-10
BACKGROUND: Diabetic wounds are a major clinical challenge, because minor skin wounds can lead to chronic, unhealed ulcers and ultimately result in infection, gangrene, or even amputation. Studies on bone marrow derived mesenchymal stem cells (BMSCs) and a series of growth factors have revealed their many benefits for wound healing and regeneration. Platelet-rich plasma (PRP) may improve the environment for BMSC development and differentiation. However, whether combined use of BMSCs and PRP may be more effective for accelerating diabetic ulcer healing remains unclear. OBJECTIVE: We investigated the efficacy of BMSCs and PRP for the repair of refractory wound healing in a diabetic rat model. METHODS: Forty-eight rats with diabetes mellitus induced by streptozotocin were divided into four groups: treatment with BMSCs plus PRP, BMSCs alone, PRP alone, phosphate buffered saline. The rate of wound closure was quantified. A histopathological study was conducted regarding wound depth and the skin edge at 7, 14, and 28 days after surgery. RESULTS: Wound healing rates were significantly higher in the BMSC plus PRP group than in the other groups. The immunohistochemistry results showed that the expression of platelet/endothelial cell adhesion molecule 1, proliferating cell nuclear antigen, and transforming growth factor-beta1 increased significantly in the BMSC plus PRP group compared to the other treatment groups. On day 7, CD68 expression increased significantly in the wounds of the BMSC plus PRP group, but decreased markedly at day 14 compared to the controls. CONCLUSION: The combination of BMSCs and PRP aids diabetic wound repair and regeneration.
Amputation
;
Animals
;
Bone Marrow
;
Cell Adhesion
;
Diabetes Mellitus
;
Gangrene
;
Immunohistochemistry
;
Intercellular Signaling Peptides and Proteins
;
Mesenchymal Stromal Cells*
;
Models, Animal
;
Platelet-Rich Plasma*
;
Proliferating Cell Nuclear Antigen
;
Rats*
;
Regeneration
;
Skin
;
Streptozocin
;
Ulcer
;
Wound Healing
;
Wounds and Injuries
6. A retrospective comparative study of continuous pumping for home enteral nutrition after esophagectomy
Liang DAI ; Hao FU ; Xiaozheng KANG ; Yongbo YANG ; Haitao ZHOU ; Zhen LIANG ; Hongchao XIONG ; Yao LIN ; Keneng CHEN
Chinese Journal of Surgery 2018;56(8):607-610
Objective:
To discuss the effect and safety of continuous pumping for home enteral nutrition after esophagectomy.
Methods:
The current study retrospectively analyzed the esophageal cancer patients who underwent transthoracic esophagectomy between January 2017 and November 2017 at First Department of Thoracic Surgery, Peking University Cancer Hospital and Institute. There were totally 108 cases, including 88 males and 20 females, with an average age of 62 years. The patients were divided into pump feeding group (
7.Correlation analysis of occupational burnout and social support for medical staff in the community in the context of normalized epidemic prevention and control
Xiong XIONG ; Yongbo LIN ; Changcai ZHU ; Jing ZHOU
Journal of Public Health and Preventive Medicine 2021;32(4):113-115
Objective To explore the current status and relevance of job burnout and social support among medical staff in the community of Hospitals under the control of Epidemic prevention and control and to provide scientific basis for targeted intervention measures. Methods The job burnout scale and social support scale were used to investigate and analyze the job burnout and social support of medical staff in Dongxihu District, Wuhan. Results Among 294 medical staff, 163 cases (55.44%) had occupational burnout, among which, mild to moderate and severe occupational burnout accounted for 46.26% and 9.18% respectively. The results of partial correlation analysis showed that the total score and various dimensions of the subject's job burnout were negatively correlated with the total score of the subject's social support, subjective support, and support utilization ( P<0.05). Conclusion In the context of normalized epidemic prevention and control of Epidemic prevention and control, medical staff suffered from severe job burnout; the social support of medical staff may affect their job burnout.
8. Comparison of disinfection effects of different disinfection methods for caps of three-porous polypropylene infusion bottles
Chengyu HE ; Zhengjuan SHI ; Fang YANG ; Yan LONG ; Xiaodong DENG ; Rong SU ; Lin YANG ; YongBo XIE ; Xiaoqing YANG ; Jing ZHANG
Chinese Journal of Practical Nursing 2020;36(3):176-179
Objective:
To compare the disinfection effect of three-hole polypropylene infusion bottle stopper by central sterilization method and groove central sterilization method, and to explore the best method of three-hole polypropylene infusion bottle cap disinfection.
Methods:
A total of 1 088 bottles of three-pore polypropylene infusion bottles from 10 hospitals in Panzhihua City and the surrounding areas of Chengdu were selected for disinfection, and the effects were compared by fluorescent powder tracing method and bacterial culture method respectively. The two methods were divided into two groups: bottle stopper Central Sterilization group and groove Central Sterilization group. Each nurse sterilized two groups of liquid.
Results:
The total area of fluorescent powder residues after disinfection of 272 bottles of infusion bottles with different specifications
9.PTMD: A Database of Human Disease-associated Post-translational Modifications.
Haodong XU ; Yongbo WANG ; Shaofeng LIN ; Wankun DENG ; Di PENG ; Qinghua CUI ; Yu XUE
Genomics, Proteomics & Bioinformatics 2018;16(4):244-251
Various posttranslational modifications (PTMs) participate in nearly all aspects of biological processes by regulating protein functions, and aberrant states of PTMs are frequently implicated in human diseases. Therefore, an integral resource of PTM-disease associations (PDAs) would be a great help for both academic research and clinical use. In this work, we reported PTMD, a well-curated database containing PTMs that are associated with human diseases. We manually collected 1950 known PDAs in 749 proteins for 23 types of PTMs and 275 types of diseases from the literature. Database analyses show that phosphorylation has the largest number of disease associations, whereas neurologic diseases have the largest number of PTM associations. We classified all known PDAs into six classes according to the PTM status in diseases and demonstrated that the upregulation and presence of PTM events account for a predominant proportion of disease-associated PTM events. By reconstructing a disease-gene network, we observed that breast cancers have the largest number of associated PTMs and AKT1 has the largest number of PTMs connected to diseases. Finally, the PTMD database was developed with detailed annotations and can be a useful resource for further analyzing the relations between PTMs and human diseases. PTMD is freely accessible at http://ptmd.biocuckoo.org.
Databases, Protein
;
Disease
;
genetics
;
Gene Regulatory Networks
;
Humans
;
Phosphorylation
;
Protein Processing, Post-Translational
;
Proteins
;
metabolism
;
Search Engine
10.Exploration of Postoperative Follow-up Strategies for Early Staged NSCLC Patients on the Basis of Follow-up Result of 416 Stage I NSCLC Patients after Lobectomy.
Liang DAI ; Wanpu YAN ; Xiaozheng KANG ; Hao FU ; Yongbo YANG ; Haitao ZHOU ; Zhen LIANG ; Hongchao XIONG ; Yao LIN ; Keneng CHEN
Chinese Journal of Lung Cancer 2018;21(3):199-203
BACKGROUND:
Currently, there is no consensus on the follow-up strategy (follow-up time interval and content) of non-small cell lung cancer (NSCLC) in the world, and the relevant clinical evidence is also very limited. In this study, we aimed to summarize the recurrence/metastasis sites and timings of stage I NSCLC patients based on their follow-up data, aiming to provide a basis of follow-up time interval and content for this group of patients.
METHODS:
We retrospectively analyzed the 416 stage I NSCLC patients that underwent continuous anatomic lobectomy between Jan. 2000 to Oct. 2013 in our prospective lung cancer database. According to the recurrence/metastasis sites and timings, the long term follow-up time interval and content were explored.
RESULTS:
The 5-yr disease free survival (DFS) and overall survival (OS) in the whole group were 82.4% and 85.4%, respectively. There were 76 cases (18.3%) had recurrence/metastasis during follow-up, among which the most frequent site was pulmonary metastasis (21 cases, 5.0%), followed by brain metastasis (20 cases, 4.8%), bone metastasis (12 cases, 2.9%), and mediastinal lymph node metastasis (12 cases, 2.9%). Among the factors that could influence recurrence/metastasis, patients with pT2a suffered from a higher recurrence/metastasis rate compared to patients with pT1 (P=0.006), with 5-yr DFS being 73.8% and 87.3%, respectively (P=0.002), and the 5-yr OS being 77.7% and 90.3%, respectively (P=0.011).
CONCLUSIONS
The commonest recurrence/metastasis sites of stage I NSCLC after anatomic lobectomy are lung, brain and mediastinal lymph nodes, the risk of recurrence/metastasis within 2 years were equal to that between 3 years and 5 years. The follow-up frequencies and content within 2 years could be adjusted according to T stages.
Adolescent
;
Adult
;
Aged
;
Aged, 80 and over
;
Carcinoma, Non-Small-Cell Lung
;
mortality
;
pathology
;
surgery
;
Female
;
Follow-Up Studies
;
Humans
;
Lung Neoplasms
;
mortality
;
pathology
;
surgery
;
Lymph Nodes
;
surgery
;
Lymphatic Metastasis
;
Male
;
Middle Aged
;
Neoplasm Staging
;
Pneumonectomy
;
Prospective Studies
;
Retrospective Studies
;
Young Adult