1.Expression and Clinical Significance of miR-224 and miR-378e in Colorectal Cancer Tissues
Lifei GAO ; Yanfeng TIAN ; Zengren ZHAO ; Lijing ZHANG ; Xinqi HE ; Yongbin PEI
Tianjin Medical Journal 2013;(8):737-739
Objective To investigate the expression and clinical significance of microRNA-224 and microRNA-378e in colorectal cancer tissues and normal mucosa adjacent to tumor lesions. Methods The gene chip technology was used to detect the different expression of miRNA in colorectal carcinoma tissues and adjacent normal tissues, which was then confirmed by real-time PCR. The relationship between the pathology and clinical data was analyzed. Results The expres-sion level of miR-224 was significantly up-regulated in tumor tissue, while miR-378e was down-regulated in tumor tissue, which was confirmed by real-time PCR. The expression of miR-224 was strongly associated with histological types, while miR-378e was strongly associated with the infiltration depth of colorectal cancer. Conclusion miR-224 is a potent tumor promoter, while miR-378e is a potent tumor suppressor. Both miR-224 and miR-378e can be used as potential colorectal cancer molecular markers.
2.Expression of plasma miR-106a in colorectal cancer and its clinical significance.
Lijing ZHANG ; Limin MENG ; Zhibin FAN ; Bo LIU ; Yongbin PEI ; Zengren ZHAO
Journal of Southern Medical University 2014;34(3):354-357
OBJECTIVETo detect plasma miR-106a level in patients with colorectal cancer (CRC) and analyze its correlation to the clinicopathological features and disease diagnosis.
METHODSmiRNA expression profiling was performed using miRNA microarray chip for 3 colorectal adenocarcinoma samples and matched normal tissues. Plasma samples was collected from 50 colorectal cancer patients for quantitative analysis of miR-106a using real-time RT-PCR using 47 plasma samples from healthy volunteer as the control. Forty plasma samples were collected from these patients 7 days after operation to examine the changes in miR-106a expression.
RESULTSmiR-106a was differentially expressed in colorectal adenocarcinoma compared to normal tissues. The plasma levels of miR-106a expression were significantly higher in the cancer patients than in the healthy control group (P=0.012). miR-106a expression significantly decreased after the operation compared with its preoperative level (P<0.01), and no correlation was found between preoperative plasma miR-106a and the clinicopathological features including lymph node metastasis and TNM stage (P>0.05). miR-106a showed a receiver operating characteristic (ROC) curve area of 66.1%, a sensitivity of 62.3%, and a specificity of 68.2% in discriminating colorectal cancer patients from the control subjects.
CONCLUSIONplasma miR-106a is up-regulated in CRC patients, suggesting its potential value for the diagnosis of CRC.
Adult ; Aged ; Case-Control Studies ; Colorectal Neoplasms ; blood ; diagnosis ; Female ; Humans ; Lymphatic Metastasis ; Male ; MicroRNAs ; blood ; Middle Aged ; Sensitivity and Specificity ; Up-Regulation
3.Case-control study on the risk factors of type 2 diabetes of Dong nationality in Western Hunan.
Liya LIU ; Lizhang CHEN ; Jinxiong DAI ; Jiaxiong CHEN ; Ying LIANG ; Tingli PEI ; Yongbin HUANG
Journal of Central South University(Medical Sciences) 2013;38(10):1057-1064
OBJECTIVE:
To explore the risk factors of type 2 diabetes mellitus (T2DM) of Dong nationality in Western Hunan, and to provide a scientific basis for the prevention and treatment of T2DM in the district.
METHODS:
In the case-control study, the subjects were divided into a T2DM group, an impaired glucose regulation (IGR) group and a normal glucose tolerance (NGT) group through oral glucose tolerance test (OGTT). A questionnaire survey was conducted, and physical measurements and the detection of blood glucose, blood lipids and serum insulin were done.
RESULTS:
Univariate analysis found significant difference in age, fasting insulin (FINS), HOMA insulin resistance index (HOMA-IRI), HOMA β-cell function index (HOMA-βC), trigalloyl glycerol (TG), high density lipoprotein cholesterol (HDL-C), systolic blood pressure, and diastolic blood pressure in the glucose metabolism among different groups (P<0.05). Body mass index (BMI), waist/hip ratio (WHR), family history of diabetes, number of physical activities per week, dinner taste, fat and protein-rich foods, fresh vegetables and fruits intake were associated with T2DM. Multifactor non-conditional ordinal logistic regression indicated that age was the risk factor of T2DM and IGR. Compared with the group whose age was≤50, the odds ratios of the groups of 50
Blood Glucose
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Body Mass Index
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Case-Control Studies
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China
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epidemiology
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Diabetes Mellitus, Type 2
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epidemiology
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ethnology
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Ethnic Groups
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Glucose Tolerance Test
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Humans
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Insulin
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Insulin Resistance
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Insulin-Secreting Cells
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Lipids
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Obesity
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Overweight
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Risk Factors
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Socioeconomic Factors
4.Red light enhances folate accumulation in wheat seedlings.
Jianwei CHANG ; Chong XIE ; Pei WANG ; Zhenxin GU ; Yongbin HAN ; Runqiang YANG
Journal of Zhejiang University. Science. B 2021;22(11):906-916
Red, white, blue, green, and yellow lights were applied to investigate their effects on folate accumulation in wheat seedlings. The different lights, especially red light, significantly increased the total folate content. Total folate showed maximum accumulation under 30 μmol/(m
5.Effect of Shexiangbaoxin Pill on vascular endothelial function in patients with primary microvascular angina
Qi HUANG ; Xiangqian SUI ; Fengchun JIANG ; Wujian HE ; Dean PEI ; Shisheng WANG ; Yongbin HU
China Modern Doctor 2024;62(8):74-78
Objective The plasma von Willebrand factor(vWF)level in patients with primary microvascular angina(PMVA)were measured to evaluate the vascular endothelial function of the patients.The change of vWF level in patients after the treatment with Shexiangbaoxin Pill were observeg.Methods Totally 69 patients who were definitely diagnosed as PMVA,They were randomly divided into conventional treatment group(33cases)and ShexiangBaoxin Pill group(36cases).The plasma vWF levels of the two groups were measured before and after treatment.Results The level of vWF before treatment in conventional treatment group was(50.93±32.98)μg/L.The level of vWF before treatment in ShexiangBaoxin Pill group was(27.45±25.02)μg/L.The level of vWF in conventional treatment group after treatment was(49.65±35.12)μg/L.The level of vWF after treatment in ShexiangBaoxin Pill group was(17.37±15.68)μg/L.The difference of vWF decrease in Baoxin Pill group after treatment(10.08±16.47)μg/L,was lower than that in conventional treatment group(1.28±12.37)μg/L,the difference is significant(P<0.05).Conclusion Shexiang Baoxin Pill has the function of protecting vascular endothelium,and PMVA patients can benefit from treatment.