Objective To study the effect of Shuxuening injection on treatment of left ventricular diastolic dysfunction.Methods Ninety cases of left ventricular diastolic dysfunction were divided into control group and test group randomly,45 cases for each group.Both groups were treated with standard management and the treatment group was given Shuxuening injection(20 mL) additionally for 2 weeks.E/A ratio,peak E,peak A,LVEF were observed before and after treatment.Result The clinical symptoms of the two groups were all improved after treatment.The test group had a higher overall effective rate than the control group.The ventricular diastolic function paramenters(E/A ratio,peak E,peak A,LVEF) in the test group were much better than that in the control group.Conclusion Shuxuening injection can improve the clinical symptoms and signs of left ventricular diastolic dysfunction.

Objective:To construct eukaryotic expression plasmids of pneumocystis carinii p55-v3 and p55-v0 antigenic genes and to identify their expression in COS-7 cells at mRNA level.Methods:Pneumocystis carinii total RNA was used as the template to amplify p55-v3 and p55-v0 antigenic gene by RT-PCR.The products were connected to pTA2 vector and then cloned in pVAX1 eukaryotic expression vector to construct recombinant plasmids as pVAX-p55-v3 and pVAX-p55-v0.After propagated in E.coli DH5α,the recombinant plasmids were transfected into COS-7 cells.After 24 h incubation,the RT-PCR was performed to identify the mRNA expression of p55-v3 and p55-v0 antigenic gene.Results:The recombinant plasmids were qualified by restrictive endonuclease digestion and sequencing.And when compared with that in GenBank,the homology of p55-v3 antigenic gene was 99.9% in nucleotides and 100% in amino acid.The homology between p55-v0 antigenic gene and the one reported previously in nucleotide and amino acid seguence were 99.8% and 100%.The results of RT-PCR confirmed that p55-v3 and p55-v0 antigenic genes were transfected into COS-7 cells successfully and the genes were expressed in the cells.Conclusion:In this study,the recombinant plasmids of pVAX-p55-v3 and pVAX-p55-v0 are conducted successfully and expressed in the COS-7 cells,which provide a basis for clarification of immunologic function of p55-v3 and study of DNA vaccine.

Male ; Humans ; Liquid Chromatography-Mass Spectrometry ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Varicocele/diagnosis* ; Biomarkers