Objective:To construct eukaryotic expression plasmids of pneumocystis carinii p55-v3 and p55-v0 antigenic genes and to identify their expression in COS-7 cells at mRNA level.Methods:Pneumocystis carinii total RNA was used as the template to amplify p55-v3 and p55-v0 antigenic gene by RT-PCR.The products were connected to pTA2 vector and then cloned in pVAX1 eukaryotic expression vector to construct recombinant plasmids as pVAX-p55-v3 and pVAX-p55-v0.After propagated in E.coli DH5α,the recombinant plasmids were transfected into COS-7 cells.After 24 h incubation,the RT-PCR was performed to identify the mRNA expression of p55-v3 and p55-v0 antigenic gene.Results:The recombinant plasmids were qualified by restrictive endonuclease digestion and sequencing.And when compared with that in GenBank,the homology of p55-v3 antigenic gene was 99.9% in nucleotides and 100% in amino acid.The homology between p55-v0 antigenic gene and the one reported previously in nucleotide and amino acid seguence were 99.8% and 100%.The results of RT-PCR confirmed that p55-v3 and p55-v0 antigenic genes were transfected into COS-7 cells successfully and the genes were expressed in the cells.Conclusion:In this study,the recombinant plasmids of pVAX-p55-v3 and pVAX-p55-v0 are conducted successfully and expressed in the COS-7 cells,which provide a basis for clarification of immunologic function of p55-v3 and study of DNA vaccine.

Objective:To evaluate the application effect of task-based teaching combined with formative evaluation in the teaching of Nursing Research. Methods:A total of 56 nursing students from Batch 2016 were selected as the research objects. The task-based teaching method and formative evaluation were applied in the teaching of Nursing Research to guide the students to participate in and complete the whole nursing research process. The changes of students' autonomous learning ability, learning behavior and innovation ability before and after the course were investigated by questionnaires, so as to evaluate the teaching effect. SPSS 24.0 was used for t test and chi-square test. Results:There were significant differences in the scores of students' independent learning ability before the course (90.43±9.45) and that after the course (94.73±9.64) ( t=-11.87, P<0.05), especially in the aspects of information ability, learning and cooperation ability ( P<0.05). There were significant differences in the score of students' innovation ability before the course (73.64±10.12) and that after the course (77.34±12.31) ( t=-3.05, P<0.05), especially in the aspects of innovative thinking ability and innovative practice ability ( P<0.05). In addition, the students' learning behavior also changed significantly, which was manifested in the increased action of preview, review and questioning. Conclusion:Task-based teaching method combined with formative evaluation can improve students' autonomous learning ability, learning behavior and innovation ability.

Objective: To explore the status and associated factors of the knowledge and health education needs about child sexual abuse of middle school students by sex, so as to provide a basis for targeted safety education of preventing child sexual abuse. Methods: From June to July 2023, 1 256 junior high school students from 2 middle schools in southern area of Shaanxi Province were selected by a convenience sampling method. A selfdesigned general information questionnaire, Childrens Sexual Assault Cognition Questionnaire, and Health Education Needs Questionnaire were used for questionnaire survey. Group comparisons were conducted using ttests, analysis of variance, and Chisquare tests. Multiple linear regression analysis was used to explore the factors related to cognitive scores of sexual assault, and binary Logistic regression analysis was used to explore the factors related to the willingness of middle school students to receive sexual assault safety education. Results: The correct response rate of the participants for knowledge of sexual assault cognition (CSA) was (80.97±12.09)%. For each item, the correct response rate ranged 46.7%-97.2%. The correct rate (78.98±12.23)% of male students knowledge of CSA was lower than female students (83.17±11.55)% statistically (t=-6.23, P<0.01). The factors influencing the participants knowledge of CSA included gender (β=0.16), mothers occupation (β=0.07) and experience of CSA safety education (β=0.10) (P<0.05). Most students (92.7%) were willing to receive CSA education. Girls (OR=1.72, 95%CI=1.06-2.77) and students who previously received CSA education (OR=6.98, 95%CI=4.44-10.96) had more willingness to receive CSA education (P<0.05). A total of 71.8% of middle school students preferred their parents as educators, while 43.4% of students chose onsite instruction as the preferred method of CSA education. Conclusions Middle school students have a strong willingness to accept CSA safety education. Knowledge of CSA and willingness to receive CSA safety education are related to gender and previous educational experience.

Objective :To evaluate diffusion-weighted imaging (DWI) sequence for detecting the perianal fistulas by comparing it with the conventional magnetic resonance imaging (MRI) sequences .Methods :MRI findings of 40 patients with the perianal fistula proved surgically and pathologically were retrospectively analyzed . The findings of the perianal fistulas on DWI , T2-weighed imaging with fat saturation (T2 WI FS ) and T1-weighed imaging with fat saturation (T1 WI FS ) contrasted-enhancement were recorded .The comparisons of DWI with T2 WI FS and T1 WI FS contrasted-enhancement for identifying the internal openings and fistulas were performed .Results :In the forty patients ,48 fistulas and 63 internal openings were found . Forty-three (89 .6% ) internal openings and 57 (90 .5% ) fistulas were found on DWI ;37 (77 .1% ) internal openings and 51 (81 .0% ) fistulas were found on T2 WI FS ;45 (93 .8% ) internal openings and 59 (93 .7% ) fistulas were found on T1 WI FS contrasted-enhancement .The comparison in accuracy shows that distinction between T2 WI FS and T1 WI FS contrasted enhancement was statistically significant ,but there has no statistically significant difference in diagnostic accuracy between T2 WI FS combined with DWI and T2 WI FS combined with T1 WI FS contrasted enhancement .Conclusions :The accuracy of plain scan including DWI for diagnosis of perianal fistula was similar with enhanced sequence .The reasonable combination of DWI and conventional sequence can further improve the applicability of the inspection ,which can be used as a routine diagnostic MRI examination sequence for perianal fistula .

Objective To carry out the comprehensive simulation and design of experiment teaching in pediatric nursing based on putting the students at the center position and evaluate the teaching effect . Methods Four classes from 6 parallel classes of undergraduate students of nursing major in 2010 Grade were chosen and randomly divided into the control group (n=90) and the experimental group (n=107).The control group received the traditional experiment teaching , and the experimental group received the comprehensive simulation and design of experiment teaching .The score of theoretical test and practical ability were evaluated , and the feeling in the comprehensive simulation and design of experiment teaching was investigated in two students.Results The score of theoretical test was (88.81 ±10.19) in the experimental group, and was (85.12 ±13.14) in the control group, and the difference was statistically significant (t=2.25, P<0.05).The scores of 8 kinds of pediatric nursing comprehensive experimental examination in the experimental group were better than those of the control group .Most students gave affirmation to the comprehensive simulation and design of experiment teaching .Conclusions The comprehensive simulation and design of experiment teaching in pediatric nursing based on putting the students at the center position can improve the students ’ learning effect .

Male ; Humans ; Liquid Chromatography-Mass Spectrometry ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Varicocele/diagnosis* ; Biomarkers