2.Development and application of rapid molecular method for detection of as-ymptomatic infection of Leishmania
Guihua ZHAO ; Kun YIN ; Weixia ZHONG ; Yong CUI ; Hongfa WANG
Chinese Journal of Schistosomiasis Control 2015;(1):45-48,52
Objective To develop a rapid molecular biological method for detection of the asymptomatic infection of Leish?mania. Methods Two pairs of primers named RV1?RV2 and K13A?K13B were selected to be the fast diagnosis primers since they were designed according to the conserved region of Leishmania kinetoplast DNA(kDNA)minicircles. The PCR amplifica?tion products of Leishmania donovani promastigote from Shandong Province were sequenced to compare their conservatism. The method was applied to detect 105 venous blood samples from healthy home canine and 7 venous blood samples from home canine suffered from Kala?azar in Heishui County of Sichuan Province,and 75 venous blood samples from susceptible population(no leishmaniasis symptoms)and 7 venous blood samples from patients in Xinjiang Kashi area in order to verify the feasibility and accuracy of the method. Results The size of PCR products was consistent with the expected fragments with high conservative among Leishmania species. The positive rates of 105 home canine samples and 75 susceptible population samples were 37.14%(39/105)and 82.67%(62/75)rspectively,and the positive rates of home canine suffered from Kala?azar and patients were all 100%(7/7). Conclusion This rapid diagnosis method is suitable for detection of asymptomatic infection of Leishmania in Kala?azar endemic areas of China with high sensitive and specific,thus it has bright perspective to be used.
3.Etiology of severe acute respiratory syndrome.
Chang-an ZHAO ; Zhong-zhi LI ; Yong-hong YANG
Chinese Journal of Pediatrics 2003;41(6):439-440
4.Exploration of the alternative splicing variants of rat phospholipase C-gamma 1 pre-mRNA.
Zhong-Ying LIU ; Shen-Qiu LUO ; Yong-Zhong ZHAO
Journal of Southern Medical University 2007;27(2):191-194
OBJECTIVETo explore the expression of phospholipase C-gamma 1 (PLC-gamma1) alternative splicing variants in rats.
METHODSAccording to the sequence of human PLCG1 splicing variant, specific primers for rat PLC-gamma1 were designed and synthesized. The rat RNA was reverse transcribed into cDNA, which was amplified using the specific primers, and the PCR products were sequenced and analyzed using BLAST and bioinformatics methods. Totally 21 rat tissue samples were examined, including the heart, liver, lung, kidney, eyeball, and brain obtained in 3 different embryonic stages, 7 different early postnatal stages, and in adulthood.
RESULTSThe result did not show that rat PLC-gamma1 had the same splicing variant (PLC-gamma1a, NM_002660) as human does.
CONCLUSIONSThe same splicing variant of PLC-gamma1 detectable in human may not exist in rats, and the pre-mRNA may undergo splicing resulting predominantly in PLC-gamma1b mRNA. Very likely, the alternative splicing site of rat PLC-gamma1 is not identical to that of human.
Alternative Splicing ; Animals ; Base Sequence ; Molecular Sequence Data ; Phospholipase C gamma ; genetics ; RNA Precursors ; genetics ; RNA Splice Sites ; genetics ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA
5.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
6.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
7.Comparative analysis of CT imaging findings on pulmonary alveolar proteinosis and pneumoconiosis
LI Zhong xue ZHAO Xia zi LI Qian chang HUANG Shi wen TANG Yong zhong QIU Kun hai
China Occupational Medicine 2022;49(06):683-686
Objective
To analyze the difference in computed tomography (CT) imaging findings between pulmonary alveolar
pneumoconiosis Methods
proteinosis (PAP) and occupational pneumoconiosis (hereinafter referred to as ). A total of 44 patients
with PAP (PAP group) and 44 patients with pneumoconiosis (pneumoconiosis group) were selected as study subjects using
Results
convenient sampling method. The CT images of these two groups were comparatively analyzed. The detection rates of
- -
pulmonary CT pattern changes such as map like performance, ground glass opacity, paving stone sign and sphenoid wing like
vs vs
changes of pulmonary hilum in the PAP group were higher than those in the pneumoconiosis group (77.3% 0.0%, 75.0%
vs vs P
2.3%, 56.8% 0.0%, 18.2% 0.0%, all <0.01); the detection rates of lymphadenopathy and calcification of pulmonary hilum,
small pulmonary nodules, emphysema and interlobular septal thickening were lower in the PAP group than those in the
vs vs vs vs P Conclusion
pneumoconiosis group (34.1% 100.0%, 4.5% 100.0%, 2.3% 45.4%, 0.0% 22.7%, all <0.01). Paving
-
stone sign and map like performance were most commonly found in the CT imaging of patients with PAP, and it is uncommon in
pneumoconiosis. These changes could be used as the CT differential diagnosis of the two diseases.
8.Research Progress in TCM Protection for Exercise Fatigue Resistance
Junlian LIU ; Yue ZHONG ; Yong YI ; Quanchun FAN ; Guie BAI ; Shuang ZHAO
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(9):134-136
Exercise fatigue is a physiological phenomenon not only for athletes, but also for astronauts. It is vital for athletes with hard training and astronauts with the heavy exercises for the mission in manned space flight to know how to solve the exercise fatigue. This article reviewed current commonly used TCM protective measures against fatigue.
9.Effects of Cdc20 mutation on growth of mouse embryonic fibroblast
Juling FENG ; Lei ZHAO ; Juan XIE ; Mingshu MO ; Qingjun GUI ; Yong YOU ; Hui ZHONG ; Lisheng WANG
Acta Anatomica Sinica 2014;(3):383-387
Objective Investigation of biological characteristics of Cdc 20AAA/+APCmin/+ mouse embryonic fibroblast(MEFs) indicate the effect of Cdc20AAA/+on growth of mouse embryonic fibroblast and the possible mechanism . Methods MEFs of Cdc20AAA/+APCmin/+, Cdc20AAA/+, APCmin/+ and WT genotype were harvested from embryos for analysis.The growth characteristics of Cdc20AAA/+APCmin/+, Cdc20AAA/+,APCmin/+and WT mouse embryonic fibroblast were analyzed through growth curve analysis and foci formation assay .Separation of sister chromatid and the presence of aneuploid were detected by karyotype analysis .Results Cell proliferation assays showed that Cdc 20AAA/+APCmin/+cells grew at an accelerated rate compared with APC min/+MEFs(P<0.01).Foci formation assay showed that the clone forming ability was significantly increased .Cdc20AAA/+APCmin/+MEFs showed a significant increase in the frequency of aneuploid compared with WT MEFs , which had a karyotype of 38 and contained prematurely separated sister chromatids .Conclusion Cdc20 carrying a null allele (Cdc20AAA/+) may accelerate the growth and proliferation of APC min/+MEFs and present the growth characteristics of the tumor cells .The possible mechanism may be associated with chromosome instability .
10.Effects of palmitate-stimulated macrophages on invasion and migration of HepG2 cells
Yan WANG ; Yong YAN ; Shan ZHONG ; Xiongzhong RUAN ; Yaxi CHEN ; Lei ZHAO
Chinese Journal of Pathophysiology 2017;33(3):495-499
AIM:To investigate the impact of palmitate-stimulated macrophages on the invasion and migration of HepG2 cells and to explore the underlying mechanism .METHODS:Human acute monocytic leukemia cell line THP-1 were induced to macrophages by phorbol myristate acetate and were stimulated with palmitate (0.16 mmol/L).The culture supernatants were collected and used to incubate HepG 2 cells.The effect of palmitate on migration of the macrophages was detected by Transwell chamber assay .The mRNA expression of target genes was measured by RT-qPCR.The invasion and migration of the HepG 2 cells were assessed by invasion assay and scratch test .RESULTS:Palmitate promoted the migra-tion of the macrophages and increased the mRNA levels of interleukin -1β( IL-1β) , interleukin-6 ( IL-6 ) , tumor necrosis factor-α(TNF-α) and monocyte chemotactic protein-1 (MCP-1) in the macrophages.The invasion and migration of the HepG2 cells incubated with conditioned media from palmitate-stimulated macrophages were greater than those of the HepG 2 cells incubated with conditioned media from macrophages without palmitate .The media of palmitate-stimulated macrophages up-regulated the mRNA expression of cytokines and N-cadherin, and down-regulated the mRNA expression of E-cadherin in the HepG2 cells.CONCLUSION:Palmitate-stimulated macrophages promote the invasion and migration of HepG 2 cells through paracrine/endcrine loop.