2.Cloning and Expression Analysis of two Endosperm Promoters in Rice
Yong-Lan CUI ; Xiao-Li ZHONG ; Yong-Ming ZHANG ; Zhong-Nan YANG ;
China Biotechnology 2006;0(08):-
The cloning of promoter is important for studying the genetic engineering and the regulation of gene expression in plants. Two promoters Os772 and Os359, which are predicted to be highly expressed in the endosperm of rice from the EST database were cloned. After construction of the Os772∶∶GUS and Os359∶∶GUS expression vectors, they were transformed into rice. X-Gluc staining of transgenic plants showed that Os772 and Os359 can promote GUS gene expression in matured endosperm but not in root, stem, leaf and flower. This result indicates Os772 and Os359 are two rice endosperm-specific promoters.
6.Continuous intravenous infusion of midazolam for treatment of status epilepticus in children.
Jian-min ZHONG ; Jian-hua LI ; Yong CHEN
Chinese Journal of Pediatrics 2004;42(4):299-300
Adolescent
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Age Factors
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Anti-Anxiety Agents
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administration & dosage
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adverse effects
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therapeutic use
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Child
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Child, Preschool
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Female
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Humans
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Infant
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Infusions, Intravenous
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Male
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Midazolam
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administration & dosage
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adverse effects
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therapeutic use
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Status Epilepticus
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drug therapy
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Treatment Outcome
8.Etiology of severe acute respiratory syndrome.
Chang-an ZHAO ; Zhong-zhi LI ; Yong-hong YANG
Chinese Journal of Pediatrics 2003;41(6):439-440
9.Biology and Future Clinical Perspectives of Adipose-tissue Derived Mesenchymal Stem Cells
Yong-Jiang MA ; Yu-Gu LI ; Zhong-Ying DOU ;
China Biotechnology 2006;0(05):-
Adipose-tissue mesenchymal stem cells, is one kind of multipotent stem cells, can differentiate into adipogenic, osteogenic, chondrogenic, myogenic cells and so on in vitro and in vivo. Human adipose tissue is plentiful, easily harvested in large quantity with little patient discomfort. Adipose-tissue derived mesenchymal stem cells may be an alternative stem cell source for mesenchymal tissue regeneration and engineering without ethical consideration of embryonic stem cells and severe pain resulted by bone marrow procurement. The research work on adipose-tissue mesenchymal stem cells and future clinical perspectives were reviewed.
10.Value of Doppler ultrasound in monitoring velocity waveforms in the fetal main pulmonary artery in patients with early onset severe preeclampsia
Yong GUAN ; Shengli LI ; Haiteng YE ; Wenjun ZHONG ; Chenhong WANG
Chinese Journal of Ultrasonography 2017;26(7):587-591
Objective To explore the change of Doppler velocity waveforms in the fetal main pulmonary artery in patients with early onset severe preeclampsia and to assess its value in fetal lung maturity.Methods A prospective study was conducted to analyze fetal main pulmonary artery velocity waveforms parameters including acceleration time(AT),ejection time(ET),acceleration time/ejection time (AT/ET),peak systolic velocity (PSV) in 30 cases of pregnant women with early onset severe preeclampsia.According to neonatal outcome,these cases were divided into respiratory distress syndrome (RDS) group (18 cases) and non-RDS group (12 cases).Each group was compared with 54 cases and 36 cases that were normal and had corresponding gestational age separately.Doppler parameters were compared between these two groups and their corresponding normal control groups.Results Gestational age at sonographic examination,gestational age at delivery and birth weight of neonates in RDS group were significantly lower than those in non-RDS group(all P <0.001).The value of AT,ET,AT/ET,PSV were lower in RDS group than those in gestational age-matched control group(all P <0.05),while there were no significant difference between non-RDS group and gestational age-matched control group(all P > 0.05).Conclusions Doppler ultrasound monitoring fetal main pulmonary artery velocity waveforms to evaluate fetal lung maturity in patients with early onset severe preeclampsia has clinical application value.