Sponge Dysidea avara-associated bacteria were isolated by dilution-plate method and the bacterial anti-microbial activities were screened for Escherichia coli,Staphylococcus aureu, Pseudomonas fluorescens, Bacillus subtilis,Aspergillus niger, Candida albicans,Pacecilomyces variotii by agar diffusion method.At the same time, morphology observations, biochemical and physiological identification of active strains were made. In this paper, 149 strains were isolated from sponge Dysidea avara.20 strains, which amount to 13.4% of the total isolated strains, were found to have anti-microbial activity against fungi and gram-positive bacteria and were identified to be the genus Bacillus sp.

Eukaryotic DNA element called Matrix Attachment Regions (MARs) can function on regulating the structure and activity of chromosome. Traditional quantitation in vitro and indirect functional analysis can not always reflect MAR-involved physiological state. In order to study transcription regulation and make a try in methodism,? 1-antitrypsin MAR (?1-AT MAR) is cloned and incorporated into pEGFP-C1 vector. Non-MAR-containing and MAR-containing plasmids were then transfected into HEK-293 cells with LipofectamineTM 2000 respectively. Positive cell clones were assayed after 20 days of selection by G418. Semiquantitative RT-PCR and fluorescence microscope analysis show that this MAR has a positive effect on modulating nearby gene expression. Further, co-localization with newly CMV promoter and RNA polymeraseⅡ(RNAPⅡ) was detected by chromatin immunoprecipitation (ChIP), The PCR result demonstrates that more RNAPⅡwas recruited to the CMV promoter to initiate transcription in presence of MAR. ChIP can be used to confirm the MAR-mediated transcriptional activation and provide more reliable information than RT-PCR in real time. The technology is also providing a platform for our research in gene expression regulation.

Adult ; Diagnostic Errors ; Female ; Humans ; Middle Aged ; Myasthenia Gravis ; diagnosis

The microbial community structure of 4 kinds of sponges in South China Sea were revealed using culture-independent PCR- DGGE gene fingerprint technique as well as the mixed culturable microorganism. The relationship between the culturable and un-culturable microorganism was studied based on the different fingerprint profile. It was found that there is host specificity for microorganism in different sponges from the same marine area. Culture condition such as medium is an important factor for sponge-associated microbial cultivation and the culturable microorganism amount to only a little part of the total sponge-associated microorganisms.

A total of 104 marine bacterial strains were isolated from Stelletta tennui around Sanya area of South China Sea by dilution-plate method and were screened for anti-microbial activity on Escherichia coli, Staphylococcus aureu, Pseudomonas fluorescens, Bacillus subtilis, Aspergillus niger, Candida albicans and Pacecilomyces variotii by agar diffusion, paper disc diffusion assay and cell concentration counts methods. It was found that 23 strains, which are 22.2% of the total isolated strains, have anti-microbial activities. Among the 23 strains, A05, A08, A72 and A75 were morphologically, physiologically and biochemically characterized and identified to be the genus Bacillus. At the same time, it was proved that there are positive and negative synergistic effects between or among active strains, e.g., as for A72-75 combination, an obvious enhanced anti-microbial activities on inhibiting Candida albicans and Pseudomomas fluorescens growth was observed than A72 or A75.

0.05),but there were statistically difference within E15,E19,P2,P10(Pa

Objective To review our clinical experience on 12 patients with retroperitoneal infection who were treated with retroperitoneal laparoscopic debridement.Method This retrospective study included 12 patients with retroperitoneal infection who were treated with retroperitoneal laparoscopic dehridement and drainage.Results All the 12 patients recovered well and were finally discharged home.Conclusions Retroperitoneal laparoscopic debridement and drainage for retroperitoneal infection is a mini-invasive procedure.It was found to be safe,produced minimal bleeding and resulted in rapid postoperative recovery.It can be used as the first choice treatment in properly selected patients.

Objective: To observe the clinical efficacy of Zhi Shen Tiao Sui (regulating the mind and marrow) needling in treating depression after ischemic stroke. Methods: A total of 145 patients with depression after ischemic stroke were recruited between January 2016 and January 2018 and divided into two groups using random number table method, with 72 cases in the control group and 73 cases in the observation group. The control group received conventional treatment for ischemic stroke and medication, while the observation group was given additional Zhi Shen Tiao Sui acupuncture. The two groups were treated for 4 successive weeks. Clinical efficacy was analyzed after treatment. The neurological function pertinent scores [Hamilton depression scale (HAMD), clinical neurological deficit score (CNDS), activities of daily living (ADL), Fugl-Meyer assessment scale (FMA) and Barthel index (BI) scores], as well as the levels of cortisol (CORT) and adrenocorticotropic hormone (ACTH) were recorded in the two groups. Adverse reactions that occurred during treatment were also observed. Results: The clinical total effective rate was 82.2％ in the observation group, significantly higher than 66.7％ in the control group (P<0.05). After treatment, the HAMD and CNDS scores were markedly reduced in both groups (all P<0.05), and the ADL, FMA and BI scores were markedly increased (all P<0.05). The HAMD and CNDS scores were significantly lower in the observation group than in the control group after treatment (both P<0.05), and the ADL, FMA and BI scores were significantly higher in the observation group than in the control group (all P<0.05). The levels of CORT and ACTH declined significantly after treatment in the two groups (all P<0.05), and were markedly lower in the observation group than in the control group (both P<0.05). The adverse reaction rate was 2.7％ in the observation group, versus 4.2％ in the control group, and the between-group difference was statistically insignificant (P>0.05). Conclusion: Compared with sole use of Western medication, medication combined with Zhi Shen Tiao Sui acupuncture can promote the recovery of neurological function, down-regulate the levels of CORT and ACTH and enhance the clinical efficacy in treating patients with depression after ischemic stroke, with a relatively high safety rating.

Objective: To access glycemic control status and investigate its impact factors of patients with short-term history of type 2 diabetes mellitus in Pujiang Town, Minhang District, Shanghai. Methods: The clinical information was collected by a standard questionnaire in 1 994 type 2 diabetic mellitus patients from the community. FPG, HbA1c, and PPG after meal of 100 g steamed bread made of standard flour were measured. 429 patients with 5-years duration of diabetes were qualified in the final analysis. Ideal blood glucose control was FPG<7.0 mmol/L, PPG<10.0 mmol/L and HbA1c<7.0%. Results: The proportion of patients with ideal blood glucose, FPG, PPG, and HbA1c control were 7.9%, 32.6%, 12.6% and 38.2%, respectively. Patients with middle school- and high school-education were at lower risks for poor glycemic control (age- and sex- adjusted OR=0.60, 95% CI 0.37-0.96). Duration of diabetes, insulin resistance and blood pressure were negatively associated with glycemic control (age- and sex- adjusted, OR=1.95, 95% CI 1.30-2.91; OR=2.52, 95% CI 1.57-4.03; OR=1.94,95% CI 1.22-3.09, respectively). Conclusion: Patients in Pujiang community had substantially poor glycemic control. Less-educated diabetic patients should be emphasized on the importance of postprandial glycemic control.

PKS gene was screened by PCR from thirty strains of spone-associated bacteria including twenty-one actinomycetes isolated from Craniella anstrialiensis and nine bacillus isolated from Dysidea avara in the South China Sea.As a result,a 669 bp KS domain gene was successfully amplified from Bacillus C89.BLAST analysis showed that the KS domains were most closely related to the KS sequences of Bacillus subtilis subsp.subtilis str.168 with 96% similarity.Phylogenetic analysis demonstrated the KS domain belong to trans-AT KS domains.This study demonstrated the existence of PKS gene in bacteria associated with sponge Dysidea avara for the first time,and provided proof for the hypothesis that sponge-associated bacteria are perhaps the true producers of many novel bioactive compounds in sponge.Meanwhile,this study lays a basis for the microbial screening for polyketide compounds production.