1.Determination of the Content of Lomustine in Mouse Tumor Tissue by RP-HPLC
China Pharmacy 2001;0(09):-
OBJECTIVE: To determine the content of lomustine in mouse tumor tissue. METHODS: Lomustine was separated and determined on a reverse-phase C18 5um column with a mobile phase of acetonitrile-H2O(54 : 46), detected at 254nm and no internal standard. RESULTS: The ca[ibration curve was 1ineaI(r = 0. 9 999) within the range of 0. 98- 15. 68ug/ml for lomus tine. The recovery ratio of lomustine was 68. 64% (n = 9). The relative standard deviation(RSD) was 0. 32%. CONCLUSION: This method is simple and accurate
2.Analysis of HIV and HCV infection of heroin dependent patients
International Journal of Laboratory Medicine 2014;(22):3044-3045
Objective To understand the situation of HIV and HCV infection of heroin dependent patients .Methods HIV and HCV serological detection were proceed among 565 heroin dependent patients ,and the results were compared according to genders and nationalities .Results Among 565 heroin dependent patients ,the infection rates of HIV ,HCV ,and HIV/HCV for males were 14 .57% (72/494) ,59 .72% (295/494) ,and 13 .36% (66/494) respectively ,and those for females were 19 .72% (14/71) ,77 .46%(55/71) and 16 .90% (12/71) respectively .The infection rates for males were significantly lower than females (P<0 .05) .There were significant difference of the infection rates of HIV ,HCV and HIV/HCV between Han patients and Uyghur patients(P<0 .05) .Conclusion The heroin dependent patients are high risk population for HIV and HCV infection .Education and publication of drugs and infectious should be enhanced in order to control the spread of disease and reduce disease incidence .
3.Pregnant outcome in women over 41 gestational weeks
Xiaowei ZHANG ; Xiao SUN ; Yong WANG
Chinese Journal of Perinatal Medicine 1998;0(01):-
Objective To study the pregnant outcome of those women who were over 41 gesta tional weeks. Methods A retrospective study, including 2903 normal nulliparous women who had delivered between July 1,2002, and December 31,2004, was conducted. Totally, 461 cases of prolonged pregnancies were included. The rates of cesarean delivery, fetal distress, and neonatal asphyxia were compared between those over 41 weeks and the others. Results The rates of cesarean delivery (48. 81%) and fetal distress (43. 38%) in prolonged pregnant group were significantly increased than those of the others women(P
4.The theory and application of fluorescence resonance energy transfer
Xuegang SUN ; Lihua ZHANG ; Yong JIANG
Chinese Journal of Pathophysiology 1999;0(09):-
The structural and functional study of protein is a major topic of current functional genomics. Fluorescence resonance energy transfer (FRET) is one of few tools available for measuring nanometer scale distances and changes in distances in vivo . FRET is an ideal technology for detection of protein conformation and protein-protein interaction by using fluorescence protein, traditional organic dyes and other dyes as probes. It uses fluorescence protein, traditional organic dyes and other dyes as its probes. The application of FRET in the determination of intracellular events would be helpful for us to understand the structure and function of biology molecules. [
5.Comparison of analgesic effects between methylene blue and adriamycin magnetic gelatin microballoons
Yonghai SUN ; Hong ZHANG ; Yong WANG
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
Objective To compare the analgesic effects between methylene blue and adriamycin magnetic gelatin microballoons on blocking the cornu dorsale medullae spinalis of rabbit,and to investigate its mechanism.Methods 40 rabbits were randomly divided into 7 groups including sham operation group,gelatin microballoon controlled group(5mg and 15mg),methylene blue microballoon group(5mg and 15mg)and adriamycin microballoon group(5mg and 15mg).Under the guide of magnet,microballoons were infused into subarachnoid space of rabbits.The pain threshold of electronic stimulation and motor function of rabbits' hind limbs were observed continuously.The somatosensory evoked potential was recorded.Results The pain threshold was raised obviously(P
6.The change in somatosensory evoked potential caused by infusing adriamycin magnetic gelatin microspheres into subarachnoid space of rabbits
Yong WANG ; Yonghai SUN ; Hong ZHANG
Medical Journal of Chinese People's Liberation Army 2001;0(07):-
Objective To study the changes in somatosensory evoked potential caused by injection of adriamycin magnetic gelatin microspheres into subarachnoid space of rabbits. Methods Thirty rabbits were randomly divided into 5 groups: sham operation group (group S), gelatin microspheres 5mg (group C1), 15mg of gelatin microsphere (group C2) controlled groups, and adriamycin microspheres 5mg (group A1) and 15mg (group A2) groups. Under the effect of magnet, microspheres were injected into the subarachnoid space of rabbits. The pain threshold of electronic stimulation, motor function of rabbits′ hinder limbs and somatosensory evoked potential (SEP) were observed continuously. Results Obvious rise of pain threshold was found in group A2 (P
7.Association between dry eye and inflammatory response mediated by CCR5 and CXCR3 and their ligands
Hong, ZHANG ; Yong, SUN ; Xiao, AN ; Xueyi, CHEN
Chinese Journal of Experimental Ophthalmology 2015;33(7):633-637
Background Sustained abnormal tear secretion in dry eye patients may lead to ocular surface and lacrimal glands in the state of long-term inflammatory cell infiltration.Lacrimal gland suffers immune attack by lymphoproliferative,and inflammation interferes normal gland secretion,in which chemokines and their receptors on lymphocytes play a key role.Objective This study was to investigate the inflammation mechanism of delayed allergy induced by Th1 cell in the development of dry eye.Methods Sixty eyes of 30 patients with dry eye and matched 30 healthy volunteers were enrolled in Affiliated First Hospital of Xinjiang Medical University from June to December in 2012.Schirmer Ⅰ test (S Ⅰ t),break up time (BUT) of tear and corneal fluorescence stain (FLS) were performed on the subjects,and conjunctiva epithelial cells were obtained using cytological method of conjunctiva imprinting.Positive cell rates of CC chemokine receptor 5 (CCR5) and CXC chemokine receptor 3 (CXCR3) were detected by flow cytology,and the relative expression levels of regulated upon activation of normal T cells exp ressed and secreted (RANTES),macrophage inflammatory protein (MIP)-lα and MIP-1β,monokine induced by interferon-γ (IFN-γ) (MIG),interferon-γ inducible protein 10 (IP10) and interferon-inducible T-cell α chemoattractant (I-TAC) mRNA were quantitatively assayed by real-time PCR.Differences of the positive rates of CCR5,CXCR3 and lignds were compared between dry eye group and normal control group.Relationship between the positive rates of CCR5,CXCR3 and BUT,S Ⅰ t,FLS scores was analyzed.Results The values of BUT,S Ⅰ t were (2.90±1.37) seconds and (4.00±2.49) mm/5 minutes in the dry eye group,which were significantly lower than (8.56±4.69) seconds and (11.31 ±5.23) mm/5 minutes in the normal control group (t =3.172,2.186,both at P<0.05).FLS scores,positive rates of CCR5 and CXCR3 were0.90±0.57,(3.38±0.66) % and (2.64±0.47)% in the dry eye group,showing significant elevations in comparison with 0.14±0.06,(2.12±0.21) % and (1.12±0.11) % in the normal control group (t=2.297,3.151,5.454,all at P<0.05).In the dry eye group,the masculine rate of CCR5 was negatively correlated with BUT and S Ⅰ t (r=-0.473,-0.385,both at P<0.05).The masculine rate of CXCR3 was negatively correlated with BUT and S Ⅰ t (r =-0.753,-0.684,both at P<0.05).No considerable correlations between the positive rate of CCR5 with the positive rate of CXCR3 or FLS scores (r =0.231,0.336,both at P<0.05).The relative expression levels of RANTES,MIP-1α,MIG and IP10 mRNA in the dry eye group were significantly higher than those in the normal control group (t =3.091,2.894,2.688,2.245,all at P<0.05),but there were no significant differences in the relative expression levels of MIP-1β and I-TAC mRNA between the two groups (t =0.512,1.979,both at P>0.05).The positive correlations were seen between the masculine rate of CCR5 with the relative expression of RANTES or MIP-1α mRNA (r=0.473,0.285,both at P<0.05),but there was no obvious correlation between the masculine rate of CCR5 and the MIP-1 β mRNA expression(r=0.214,P>0.05).In addition,the masculine rate of CXCR3 was positive correlated with the expressions of MIG and IP10 mRNA (r=0.553,0.314,both at P<0.05),whereas the masculine rate of CXCR3 was not related to the expression of I-TAC mRNA (r=0.364,P>0.05).Conclusions Dry eye is probably along with the long-term infiltration of inflammatory cells.The delayed allergy induced by Th1 cells and the nature killed cells is probably the primary cause to xerophthalmia.CCR5,CXCR3 and their ligands might be the regulative targets in the inflammation mechanism of dry eye.
8.Treatment of submucous hysteromyoma under hysteroscope
Hua SUN ; Yong ZHANG ; Jian ZHENG
Chinese Journal of Minimally Invasive Surgery 2001;0(03):-
Objective To inquire clinical effects of hysteroscopic electroresection in the treatment of submucous hysteromyoma. Methods A retrospective analysis regarding curative effects of hysteroscopic electroresection in 72 cases of submucous hysteromyoma was carried out. Results The resected myoma was 1.5~7 cm in diameter, and the number of resected myoma was 1~3. The operation time was 10~90 min (mean, 30 min). The operation was accomplished on one session in all the 72 cases. No uterine perforation, bleeding and bowel injuries took place. Postoperative follow-up for 4~20 months in the 72 cases reported a satisfaction rate of 97 2% (70/72). Out of 6 patients who had pregnancy desire, 2 were pregnant with child and 1 of them had a delivery. Conclusions Hysteroscopic electroresection in the treatment of submucous hysteromyoma is safe and effective. It can markedly improve the menstruation and elevate the pregnancy rate.
9.Content Determination of Levodopa in Levodopa Inclusion Complex by HPLC
China Pharmacy 2001;0(10):-
OBJECTIVE:To establish a HPLC method for the determination of levodopa in levodopa inclusion complex.METHODS:The separation was performed on C18,and the mobile phase consisted of methanol-0.01mol?L-1 potassium dihydrogen phosphate buffer solution(25∶75,pH 3.0)with a flow rate of 1.0mL?min-1.The column temperature was 30℃,the detection wavelength was 280 nm and the sample size was 20?L.RESULTS:A good linear relationship was obtained for levodopa within the range of 5~80?g?mL-1(r=0.999 8),and its average recovery was 100.1%(RSD=0.19%,n=15).CONCLUSION:This method is simple,rapid,accurate,and it can be used for the content determination of levodopa in levodopa inclusion complex.
10.Influence of IL-35 on occurrence and development of heart failure
Chinese Journal of cardiovascular Rehabilitation Medicine 2015;24(6):673-676
Heart failure is a syndrom of heart disease with high morbidity and mortality .There are more and more evidence indicating immune activation developing important role in the occurrence and development of heart failure . Interleukin (IL)‐35 ,known as a negative regulatory factor of one of the members of IL‐12 family ,participates pathogenetic process of heart failure .The present article made a review for influence of IL‐35 on heart failure .