Objective To compare and analyze the difference of ?-tubulin and aurora-A expression in human cervical cancer cells (CasKi ) and immortalized human cervical squamous H8 cells with positive HPV16 E6E7.Methods Difference of ?-tubulin and aurora-A expression in CasKi and H8 cells was analyzed by showing the fluorescence intensity of ?-tubulin with indirect immunofluorescence.Expression level of aurora-A mRNA was detected by RT-PCR.Expression level of ?-tubulin and aurora-A in CasKi and H8 cells was semi-quantitatively analyzed by Western blot.Results The immunofluorescence signal of ?-tubulin was stronger in Caski cells than in H8 cells (57.78?3.13 vs 37.37?2.37,P

Objective To compare the effects of different dose aprotinin on cardiopulmonary bypass induced inflammation and myocardial injury Methods Eighteen adult patients undergoing cardiopulmonary bypass (CPB) were divided into three groups (n=6): control,low dose aprotinin and full dose aprotinin Plasma levels of tumor necrosis factor ?(TNF?), interleukin 6(IL 6), interleukin 10(IL 10), intercellular adhesion molecule 1(ICAM 1), troponin T(TnT) and neutrophil integrin cluster of differentiation 11b(CD11b) were measured at following points: before CPB(T1);1 h of CPB (T2) ;30 min after weaning from CPB (T3); 4 h after weaning from CPB (T4) ; and 24 h after weaning from CPB(T5) Results TNF?: the values in two aprotinin using groups were lower than the control levels at T2, T3 and T4 , furthermore, in full dose group was lower than in low dose group at T4 , and lower than two other groups at T5 (P

[Objective]To investigate the value of 16-slice spiral CT(MSCT)postprocessing in styloid process in vivo and provide the vivisection data for diagnosing and curing styloid process syndrome.[Method]Totally 100 volunteers with normal styloid processes underwent 16-slice spiral CT(MSCT). Based on the initial datum,4 kinds of postprocessing including multiple planar reconstruction(MPR), curve planar reconstruction(CPR),surface shaded display(SSD) and volume rendering (VR) were performed.The length and inclination angle of stytoid processes were measured. The distance of between styloid processes and carotid canal external aperture,jugular foramen, stylomastoid foramen ,mastoid process, internal carotid artery and internal jugular vein were surveyed. [Result]The average length of styloid processes was 24.51 mm?3.05 mm, left was 24.33 mm?3.15 mm and right was 24.69 mm?2.95 mm. The anterior inclination angle was 19.35??2.92? and medial was 24.00??4.07?.The distance of between styloid processes and carotid canal external aperture, jugular foramen,stylomastoid foramen ,mastoid process , internal carotid artery and internal jugular vein were 8.53 mm?2.45 mm, 2.87 mm?0.88 mm, 2.34 mm?0.74 mm, 17.23 mm?1.85 mm,6.49 mm?1.58 mm and 3.70 mm?1.46 mm respectively. [Conclusion]Sixteen-slice Spiral CT postprocessing demonstrate the stytoid processes in vivo accurately and fully, among which multiple planar reconstruction and surface shaded display are more valuable, because they can evaluate the length and inclination of the styloid processes and the distance between the processes and their surrounding structures in vivo more accurately.

[Objective]To investigate the clinical value of computed radiography(CR),computed tomography(CT)and magnetic resonance imaging(MRI)in spinal tuberculosis.[Method]Forty-one patients with histologically confirmed spinal tuberculosis underwant imaging examinations.Forty-one patients were performed CR,22 CT and 31 MRI.[Result]CR of 38 patients clearly revealed bone changes and narrowed or disappeared disk space.CR of 30 patients well showed the abnormal soft tissue.CT scan of 22 patients clearly depicted calcification and no abnormal was found in pedicles in all the 22 CT scans.CT scan of 21 patients revealed bone changes of vertebral bodies and CT scan of 20 patients showed abnormal soft tissue.The soft tissue was involved in all 31 patients with spinal tuberculosis.Diskspaces were involved in 29 cases and the pedicles were normal in all 31 case.The lesions showed hypointense in T1 scan primarily,hyperintense in T2 scan primarily.[Conclusion]CR is the first imaging approach for spinal tuberculosis.CT well reveal the extent and minute calcification.The extent,abnormal soft-tissue and abnormal disk space are more dramatically demonstrated on MRI imaging.CR,in addition to CT and MRI,is helpful in diagnosis of spinaltubereuinsis.

AIM: To evaluate the quality of Rhizoma gastrodiea processed by different drying methods. METHODS: Vacuum freeze drying and oven drying were adopted. The content of gastrodin was determined by RP-HPLC. RESULTS: The content of gastrodin of Rhizoma Gastrodiae processed by vacuum freeze drying was significantly higher than that by boil with water and oven drying. CONCLUSION: The method of vacuum freeze (drying) can prevent Rhizoma Gastrodiae from degradation of gastrodin, to keep the appearance, to make Rhizoma Gastrodiae convenient for further processing.

ObjectiveTo investigate the effects of controlled subcutaneous analgesia(PCSA) with sufentanil in patients underwent abdominal surgery and the occurrence of adverse reactions. Methods90 patients underwent abdominal surgery were randomly divided intothree groups with 30 cases each. Group Ⅰ :sufentanil 0. 04μg · kg-1 ·h-1 for postoperative analgesia; Group Ⅱ : sufentanil 0. 06μg · kg-1 · h-1 for postoperative analgesia; Group Ⅲ :sufentanil 0.08μg/kg · h for postoperative analgesia;PCSA started at the end of operation with catheter buried in deltoid muscle. Visual analgesia scale(VAS) ,Rasmay scale and side effects were recorded at 4,8,16,24 and 48h after operation. ResultsVAS scores decreased significantly in group Ⅱ and group Ⅲ compared with that in group Ⅰ ( all P ＜ 0.05) ;There were no differences of Ramsay scores between groups ( all P ＞ 0. 05) ;There were three cases of respiratory depression in group Ⅲ. ConclusionPCSA with sufentanil (0.06μg/kg) had exact analgesic effects for postoperative abdominal surgery patients with less adverse reactions.

Objective To investigate the effect of continuous positive airway pressure (CPAP) on seizure frequency in epilepsy patients complicated with obstructive sleep apnea (OSA). Methods Subjects were divided into CPAP group (20 subjects) and medication group (22 subjects) according to whether they can tolerate CPAP. CPAP group were treated with CPAP combined with antiepileptic drugs. Medication group were treated with antiepileptic drugs alone. Seizure frequency and apnea-hypopnea index (AHI) were compared between groups before and after 4 weeks and 24 weeks of treatment. Results Baseline seizure frequency and apnea-hypopnea index (AHI) were compared between groups(P>0.05). A sig?nificant reduction of seizure frequency was observed in CPAP group after 4 weeks and 24 weeks of treatment compared to that before treatment (P<0.01). In medication group, no significant difference in seizure frequency was noted betweem 4 weeks and 24 weeks after treatment compared to that before treatment(P>0.05). A significant reduction of AHI was ob?served in the CPAP group after 24 weeks of treatment compared to that before treatment (P<0.01), but no change of AHI by treatment was observed in medication group (P>0.05). Conclusion Treatment of OSA in patients complicated with epilep?sy may improve seizure control in short and long term, but longer observation time and more samples are needed for further research.

Objective To summarize the experience of double layered anastomosis in thoracoscopic and laparoscopic esophagectomy,and to explore its impact on the postoperative anastomotic complications.Methods Patients with thoracoscopic and laparoscopic esophagectomy from September 2014 to Auguest 2015 were retrospectively included.The cervical anastomosis were conducted by hand-sewn double layered anastomosis on the posterior wall of the gastric remnant,with the anastomotic configuration of end-to-side.The patients' general information and postoperative complications were recorded and analyzed.Results 45 patients with esophageal squamous cell carcinoma were included.The major postoperative complications were gastric dilatation(6/45,13.3％),hoarseness(5/45,11.1％),anastomotic leak/gastric necrosis(2/45,4.4％),anastomotic stricture(0/45,0).All patients were discharged from hospital with no perioperative death.Conclusion Hand-sewn double layered anastomotic technique could be safely used in thoracoscopic and laparoscopic esophagectomy,which could assure the security of the anastomosis.The anastomotic complication rates for this technique are rare enough to be recommended,as compared with other anastomotic methods reported in the literatures.

Myeloid derived suppressor cells(MDSCs) are a subpopulation of immunosuppressive innate immune cells derived from bone marrow stem cells,which negatively regulate immune response in tumor progression.MDSCs have a powerful suppressive function and significant heterogeneity.They can modulate innate and adaptive immune responses through a variety of mechanisms to promote the development of tumor,and also facilitate angiogenesis and metastasis of tumor via non immunologic mechanisms.In recent years,as increasingly mature research for the differentiation,proliferation and suppressive function of MDSCs,the derived researches of MDSC-targeted tumor immunotherapy will finally contribute to increasing the efficiency of vaccine and tumor therapy in the future.

OBJECTIVE: To explore the association between low flow rate and reperfusion injury during the process of preserving liver by cold machine perfusing perfluorochemical oxygen carder solution. METHODS: Forty-four male adult Wister rats were randomly divided into normal, control, experimental Ⅰ and experimental Ⅱ groups. In the normal group, the removed liver was performed isolated reperfusion guided by Clavien method. In the other 3 groups, the removed liver was infused through the portal vein by the 4-5 ℃ conserved perfluorochemical oxygen carder solution. The infused rate was controlled at 0.4, 0.2, 0.1 mL/(min·g) with 18 hours perfusion, After that, isolated reperfusion was performed. The activity of aspartate aminotransferase, alanine transaminase and endotheiin mass concentration of the effluent was detected at minutes 10, 30, and 60 after reperfusion. The histopathological changes of liver under light and election microscopy were also observed. RESULTS: The activity of aspartate aminotransferase, alanine transaminase and endothelin mass concentration had no remarkable differences between the experimental Ⅰ group and control group (P > 0.05). The differences between the experimental Ⅱ group and control group were remarkable (P < 0.05). The light and electron microscopy showed that the histopathological changes of liver in the control and experimental Ⅰ groups was lightener than experimental Ⅱ group. CONCLUSION: During the process of preserving liver by cold machine perfusion, the rate of 0.1 ml/(min·g) perfluorochemical oxygen carder solution increase the injury of hepatocyte and sinusoidal endothelial cells, which eventually result in severity of reperfusion injury.