1.Application of Luminex xMAP technology in infectious diseases.
Chinese Journal of Virology 2010;26(2):158-161
Communicable Diseases
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diagnosis
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Humans
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Influenza A Virus, H1N1 Subtype
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genetics
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Influenza, Human
;
diagnosis
;
virology
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Microspheres
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Nucleic Acid Amplification Techniques
;
methods
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Nucleic Acid Hybridization
;
methods
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Oligonucleotide Probes
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genetics
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Polymerase Chain Reaction
;
methods
;
Sensitivity and Specificity
2.Clinical observation of head and body acupuncture and moxibustion on stroke
Bo-qing WANG ; Jian-hua ZHAO ; Yong-mei ZHANG
Chinese Journal of Rehabilitation Theory and Practice 2004;10(8):490-491
ObjectiveTo observe effect of head and body acupuncture and moxibustion on stroke.Methods183 stroke patients were randomly divided into 3 groups, head acupuncture and moxibustion, body acupuncture and moxibustion, and head body acupuncture and moxibustion. After two months treatment, effects of 3 groups were evaluated.ResultsThere were no differences between head group and body group, body group and head body group, but there was significantly difference between head body group and head group (P<0.05).ConclusionThe head body acupuncture and moxibustion can gain the best clinical effect on stroke patients compared with simply head or body acupuncture and moxibustion.
3.Primary epithelioid sarcoma of the scalp: report of a case.
Lei YANG ; Yan-Qing DING ; Tong ZHAO ; Yong-Jian DENG
Chinese Journal of Pathology 2007;36(12):861-862
Adolescent
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Adult
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Head and Neck Neoplasms
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diagnosis
;
pathology
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Humans
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Magnetic Resonance Imaging
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Male
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Meninges
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pathology
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Sarcoma
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diagnosis
;
pathology
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Scalp
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pathology
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Skin Neoplasms
;
diagnosis
;
pathology
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Skull
;
pathology
4.Three successful cases treated with moxibustion and embedding needle at auricular points.
Yong-Qing LIN ; Ming chang ZHENG ; Bai-Xiao ZHAO
Chinese Acupuncture & Moxibustion 2013;33(6):571-572
Acupuncture Points
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Acupuncture, Ear
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Adult
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Dizziness
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therapy
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Headache
;
therapy
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Humans
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Male
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Middle Aged
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Moxibustion
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Rhinitis, Allergic, Seasonal
;
therapy
5.Expression of SGK in uterine decidua of patients with unexplained recurrent spontaneous abortion
Qing BAO ; Yong ZHAO ; Junqin YAN ; Wensong LIN ; Wei LIU
Chinese Journal of Primary Medicine and Pharmacy 2014;21(13):1947-1949
Objective To observe the expression of SGK in decidua in patients with unexplained recurrent spontaneous abortion(URSA) and to investigate its role in the course of apoptosis.Methods The expression of SGK was detected by immunohistochemistry in decidua of URSA(abortion group,n =50) and normal first trimester pregnant women(control group,n =30).The apoptotic index was detected by TUNEL.Results Compared with the control group,the positive expression of SGK decreased significantly in the abortion group,and the difference was statistically significant(x2 =6.78,P < 0.05).The average apoptotic index of the abortion group was (8.19 ± 3.58) %,which was significantly higher than (2.87 ±1.07)% of the control group(t =7.94,P<0.05).Conclusion Intensive apoptosis,the decreasing positive expression rate of SGK in decidua cells may play critical roles in URSA.
6.The clinical value of detecting the interleukin-6 levels in serum in the patients with sepsis
Youcheng ZHAO ; Qiong NAN ; Qing KE ; Hongjian WEI ; Yong DUAN
Journal of Chinese Physician 2001;0(05):-
Objective To investigate the regularity and significance of serum interleukin-6(IL-6) levels in the patients with sepsis.Methods Forty sepsis patients with APACHEⅡ scores over 12 and 30 normal controls were involved in the study.The IL-6 levels in serum were detected with radio-immunity analysis assay and the correlations among the IL-6 levels,the prognosis and APACHEⅡ score were investigated.Results In the patients with sepsis,the average level of IL-6 in serum was(152.02?55.77) pg/ml,which was significantly higher than that of the control group [(85.79?30.96) pg/ml](P
7.Low-density lipoprotein cholesterol levels and lipid-lowering treatment status among young and middle-aged ultra-high-risk patients with acute coronary syndrome in China.
Jun LIU ; Dong ZHAO ; Yong Chen HAO ; Na YANG ; Zhao Qing SUN ; Jing LIU
Chinese Journal of Cardiology 2022;50(12):1161-1168
Objective: To assess low-density lipoprotein cholesterol (LDL-C) levels and use of lipid-lowering treatment among young and middle-aged ultra-high-risk patients with acute coronary syndrome (ACS) in China. Methods: The study was based on the"Improving Care for Cardiovascular Disease in China (CCC)-ACS"project, a collaborative registry by and Chinese Society of Cardiology (CSC) and the American Heart Association. Hospitalized-patients with ACS were consecutively enrolled from 159 tertiary and 82 secondary hospitals across China, related clinical information was collected. This study included young and middle-aged hospitalized patients (18-59 years) with ACS from November 2014 to December 2019 registered in CCC-ACS project. Ultra-high-risk was defined according to Chinese expert consensus on lipid management of ultra-high-risk atherosclerotic cardiovascular disease (ASCVD) patients of CSC. The mean LDL-C levels at admission, pre-hospital lipid-lowering therapy and proportion of patients with LDL-C target achieved were analyzed. Results: A total of 42 230 patients younger than 60 years with ACS were included in this study. The mean age was (50.4±6.9) years, and 86.8% (36 676/42 230) of the ACS patients were male. Among them, 86.9% (36 687/42 230) met the criteria of ultra-high-risk. The mean level of LDL-C at admission was (2.8±1.0)mmol/L, only 5.3 % (1 948/36 687) patients achieved the targeted goal of LDL-C<1.4 mmol/L. Among the ultra-high-risk ASCVD patients, 17.5% (6 430/36 687) received lipid-lowering drugs before hospitalization, 96.4% (6 198/6 430) of whom received statins monotherapy. Among patients receiving pre-hospital statins, only 9.9% (626/6 323) patients reached an LDL-C<1.4 mmol/L at admission. Conclusions: The majority of young and middle-aged hospitalized patients with ACS are ultra-high-risk patients for ASCVD in China. Pre-hospital lipid-lowering drugs use is lower in these ultra-high-risk ASCVD patients and most patients do not reach the new LDL-C target level at admission.
Middle Aged
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United States
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Humans
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Male
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Adult
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Female
;
Cholesterol, LDL
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Acute Coronary Syndrome/drug therapy*
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Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use*
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China
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Atherosclerosis/drug therapy*
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Hypolipidemic Agents/therapeutic use*
8.Effect of monosialo-gangliosides on cerebral palsy of childen
Qing-hua ZHANG ; Yong ZHAO ; Yong WU ; Xiuling SUN ; Xueqin LI
Chinese Journal of Rehabilitation Theory and Practice 2004;10(7):425-426
Objective To observe the therapeutic effectiveness of monosialo gangliosides injection (GM-1) on cerebral palsy (CP) of children.Methods 87 children with CP were randomly divided into the observation group (51 cases) and control group (36 cases), and treated with physical therapy, acupuncture, massage and hyperbaric oxygen, but monosialo gangliosides was added to the observation group only. The development quotient (DQ) of each group was calculated by Gesell development schedules before and after the therapy respectively. Therapeutic effects of two groups were compared.Results The therapeutic effect of the observation group was more satisfactory than that of control group(P<0.05). The observation group expressed higher DQ and scores of gross motor, fine motor, language and reaction than that of control group. Conclusion Monosialo ganglioside is effective on CP.
9.Systems biology and traditional Chinese medicine (TCM) modernizatin.
Ji-yong ZHANG ; Qing-guo WANG ; Zhao WANG
China Journal of Chinese Materia Medica 2005;30(22):1725-1766
Systems biology is a new field in biology that aims at system-level understanding of biological systems. Based on the remarkable progress from molecular biology, systems biology focuses its research on investigating the biological systems to form their entirety. Considering the trend of TCM modernization and the coherence on the point of holism between systems biology and TCM, a comprehensive way for the research of TCM modernization was referred, which involves a new concept, componentomics.
Drug Combinations
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Drugs, Chinese Herbal
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Medicine, Chinese Traditional
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trends
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Models, Biological
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Neural Networks (Computer)
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Systems Biology
;
methods
;
trends
10.Validation of GlobalFiler® PCR Amplification Kit and the STR Polymorphism.
Zhi-yong LU ; Lu-yan XUE ; Qing-xia ZHANG ; Yi ZHAO ; Jin-jie LIU ; Hui TANG
Journal of Forensic Medicine 2015;31(4):273-276
OBJECTIVE:
To test the technical parameters of GlobalFiler® PCR Amplification Kit for its application to forensic application value and to investigate the genetic polymorphisms.
METHODS:
The validation was conducted in sensitivity, mixed samples, species specificity, adaptability, survivability, consistency, peak height balance and stability. The amplification and detection of the genomic DNA from 373 unrelated individuals from Beijing Han nationality were extracted by automation workstation.
RESULTS:
Global-Filer® PCR Amplification Kit was adaptive to some mixed, degraded and inhibited samples. The power of sensitivity and adaptability and peak height balance showed well. The distributions of genotype frequencies for 21 STR loci in the population were all in accordance with Hardy-Weinberg equilibrium (P > 0.05). The PIC value of the 21 STR loci was among 0.536 to 0.940; the H value was among 0.558 to 0.933; the DP value was among 0.783 to 0.992; the PE value was among 0.243 to 0.874.
CONCLUSION
GlobalFiler® PCR Amplification Kit is suitable for criminal cases and DNA database in forensic practice. And 21 STR loci in Beijing Han nationality have high polymorphism, which have application value in forensic practice and population genetics.
Asian People/genetics*
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Beijing
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Databases, Nucleic Acid
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Ethnicity
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Gene Frequency
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Genetic Loci/genetics*
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Genetics, Population
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Genotype
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Humans
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Polymerase Chain Reaction/standards*
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Polymorphism, Genetic
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Reproducibility of Results
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Species Specificity