ObjectiveTo evaluate the efficacy of the high-flux dialysis on maintaining hemodialysis patients. Methods60 hemodialysis patients were randomly divided into the ordinary PMMA membrane dialyzer group,the ordinary polysulfone membrane dialyzer group and the high-flux dialyzer group with 20 patients in each group.Before and after the single hemodialysis,the removing effect of serum β2- microglobulin (β2-MG)in the 3 kinds of dialyzer,and cholesterol,triglyceride,low density lipoprotein changes after 6 months were compared. ResultsThe ordinary PMMA membrane dialyzer group and high-flux dialyzer group can reduce the concentration of β2-MG,while the high-flux dialyzer group showed more significant effect.On the aspect of lipid metabolism,in the high-flux dialyzer group,serum total cholesterol,triglycerides and LDL levels decreased significantly,while the ordinary dialysis group had no significant change. ConclusionsThe high-flux dialysis can improve the dialysis efficiency,life quality and reduce the complications of patients.

Antiviral Agents ; therapeutic use ; Biomarkers ; blood ; Biopsy ; Diagnostic Imaging ; methods ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Hepatitis B, Chronic ; complications ; diagnosis ; pathology ; Humans ; Liver ; pathology ; Liver Cirrhosis ; diagnosis ; drug therapy ; pathology ; Reproducibility of Results

Objective　To find out the infection of brucella among local people.Methods　To implement requirement of brucella surveillance program according to the prevention and cure of brucella document.From 1996-1998,continous surveillance of the group people aged 7～60 years was done.Results　Three years surveillance showed that in Yuli county the brucella average infection rate is 13.11%,the rate of contracting disease is 2.62%.Such disease happened among different profession and different kinds of people.It shows that the brucela infection has transferred and diffused from pasturing area to rural section and town.Conclusions　The ways is to adopt the available prevention and cure ways,to avoid the source of brucella infection,to strengthen the prevention and cure of the brucella infection among the animals,to improve people's protection consciousness,to reduce the harm that disease done to people.

One of the strain of bivalent HFRS vaccine, Z37 strain was isolated from Rattus norvegicus and identified as SEO virus by serological test. The M segment cDNA of Hantavirus Z37 strain was obtained by reverse transcripti on and polymerase chain reaction, subsequently cloned into pGEM-T vector. The s equence of positive recombinants was determined by the method of dideoxy chain t ermination, which revealed that the M genomic segment is 3651 nucleotide in len gth with a predicated long open reading frame encoding a protein of 1133 amino acids. Comparison with HNT type (76-118, A9, HV-114 strains) indicated that th ere were 71.8%～72.1% homology at the nucleotide level, 76.2～76.7% homology at the amino acid level. Comparison with SEO type (R22,L99,80-39 strains) showed 95.3～96.1 homology at the nucleotide level, 95.3～99.2% homology at the amino acid level. The results of nucleotide and amino acid comparison indicated that Z 37 strain is SEO viruses in molecular level.

Objective To investigate the mastery degree of GCP (Good Clinical Practice) in Guang’anmen Hospital of China Academy of Chinese Medical Sciences (hereinafter referred to as“Guang’anmen Hospital”). Methods Totally 338 clinical researchers, covering 18 clinical professional sections and medical clinical trial institutions, in Guang’anmen Hospital with both qualification of clinical trials and GCP certificate were investigated in 23th-27th June 2014. The investigation was in the written answer sheet form, including four noun explanations and 36 multiple choice questions, which were related to GCP. Results 338 researchers could accurately explain the concepts of 4 nouns, and 57 (16.86%) researchers could do all multiple choice questions correctly. 141 (41.72%) researchers chose 1 wrong answer and 89 (26.33%) researchers chose 2 wrong answers. 39 (11.54%) researchers failed to answer 3-4 questions. Only 12 (3.55%) researchers failed to answer 5 or more questions. Conclusion The mastery degree of clinical researchers with qualification of clinical trials and GCP certificate in Guang’anmen Hospital is satisfied. In the future, pertinence and diverse training should be strengthened, in order to improve the overall level of the mastery degree of GCP.

BACKGROUND: Malnutrition and overnutrition are the two problems during growth and development of high-school students.OBJECTIVE: To understand the situation and relevant factors of malnutrition and overnutrition of high-school students.DESIGN: Transverse investigation was designed.SETTING: Health Supervision Institute of Chongqing Health Bureau; Department of Nutrition & Food Hygiene, Chongqing University of Medical Sciences; the Eighth Middle School of ChongqingPARTICIPANTS: Totally 896 students in 15 classes were collected from one full-time middle school in Shapingba district in Chongqing by cluster sampling method from March to May 2004. The students were excluded if they suffered from hereditary metabolic disease and secondary pathological malnutrition or obesity due to trauma or other diseases. All of participants were in the known in the investigation. Of those, 826 students filled up the questionnaire in detail (the effective rate was 92.19%), in which, 414 students were male and 412 students female, aged varied from 13 to 18 years.METHODS: The students chose were in the investigation with questionnaire and the contents of it included basic physical situation, state of sickness, situation of parents, dietary behavior, nutritional knowledge, dietary habits, etc. The students filled up the questionnaire by them own and handed over on the spot. The evaluation of student nutrition state adopted the heights and body masses in recent physical examination scale, in reference to the criteria in "height and body mass of Chinese students aged from 7 to 22 years". The standard body mass was 80% of body masses of people with same height, based on which, the standard body mass was 100%, that less than the standard by 90% was taken as malnutrition and more than it by 120% was taken as obesity. Non-conditional logistic stepwise regression was used to analyze the data so as to probe into the factors of malnutrition and obesity in middle-school students.MAIN OUTCOME MEASURES: [1] Morbidity of malnutrition and obesity in middle-school students. [2] Factors of malnutrition and obesity in middle-school students.RESULTS: Totally 826 students entered result analysis. [1] Morbidity of malnutrition and obesity: it was 9.44% and 24.21% respectively, in which,the morbidity of malnutrition.for female was higher than male [4.84%,14.08% (x2=15.1036, P ＜ 0.05)] and the morbidity of obesity in male was higher than female [30.09%, 18.2% (x2=10.5737, P ＜ 0.05)]. [2] Factors of malnutrition and obesity: significant correlation was present between malnutrition and 5 factors, named sex, indulgence in or being choosy food,sleeping time, breakfast and animal internal organs (P ＜ 0.05-0.01). Significant correlation was present between obesity and 5 factors, named sex,body mass index of father, eating velocity, midnight snack and indulgence in or being choosy food (P ＜ 0.05-0.01).CONCLUSION: It is discovered in the investigation that the morbidity of malnutrition and obesity in middle-school students are very high and that is all closely related to sex and indulgence in or being choosy food. In order to correct malnutrition and obesity in middle-school students, it is necessary to provide correct nutritional knowledge and help them arranging diet rationally and establishing healthy life pattern.

Objective　To evaluate the changes and clinical s ignificances of serum growth hormore (GH) and GH binding protein (GHBP) in patie nts with obstructive jaundice (OJ). Methods　Serum levels of GH, GHBP, insulin-like growth factor-Ⅰ (IGF-Ⅰ), endotoxin, prealbumin concentr ation and liver function in 60 male in patients with OJ were detected 1 d preope ratively and 1, 3, 7 d postoperatively. And 40 male patients with simple gallbla dder stone hospitalized in the same period were served as control. Resu lts　The levels of GH, GHBP and IGF-Ⅰ were significantly lower in pati ents with obstructive jaundice than in control group(P<0.01), which was corr elated with the levels of bilirubin, endotoxin and prealbumin (P<0.01). Conclusion　The obviously downregulation of GH/GHBP axis in patients with OJ probably results from the inbibition of expression of GH receptor due t o nutritional dysfunction and endotoxemia, and further aggravate the nutritional dysfuction and endoxemia, which may be one of the important factors in the path ophysiological changes of OJ.

Objective To investigate the variation of lipid profile in patients with acute myocardial infarction (AMI) of different age groups.Methods Total 1214 patients of AMI were admitted to our hospital from May 2007 to July 2011.The age of patients ranged 40-89 years,and patients were divided into 5 age groups with 10-years step.Differences in lipid levels were observed by using pair-wise comparison among different age groups.Results Pair-wise comparisons among different age groups indicated that TC levels were higher in 40-69 years age groups [(4.71 ± 1.08),(4.80 ± 1.30),(4.69 ± 1.03) mmol/L,respectively] than those in the 70-89 age groups [(4.41 ± 0.96),(4.38 ± 1.03) mmol/L],all P ＜ 0.05(t values were 2.381,2.323,4.382,3.965,3.317,3.096).TG levels among three age groups ＜ 59 years had no significant difference,which were higher than those in groups over 60 years.Among 50-89 years age groups,TG gradually reduced with increase of age.HDL levels were lower in 40-59 years age groups [(1.03 ± 0.23),(1.05 ± 0.26) mmol/L,respectively] than those in 60-89 years age groups [(1.11 ±0.28),(1.11±0.28),(1.16±0.34) mmol/L,respectively],allP＜0.05 (t values were-2.777,-2.789,-3.731,-2.543,-2.566,-3.644).Total cholesterol (TC) (r =-0.123,P ＜0.01),lowdensity lipoprotein cholesterol (LDL) (r =-0.139,P ＜ 0.01) and triglyceride (TG) (r =-0.287,P ＜0.01) were negatively but high-density lipoprotein cholesterol (HDL) (r =0.125,P ＜ 0.01) was positively correlated with age of patients.TC levels in female patients were higher than those in male patients in 50-89 years age groups; LDL levels in female patients were higher than those in male patients in 50-69 years age groups and HDL levels in female patients were higher than those in male patients in 40-79 years age groups (all P ＜ 0.05,t values were-2.878,-3.007,-3.352,-3.333).Conclusions Results show that the non-elderly AMI patients (＜60 years) have higher levels of TC,LDL and TG,and lower level of HDL than the elderly patients; the TC,LDL and HDL levels are higher in female AMI patients than those in male patients at the same age groups.

Objective To investigate the influence of transient receptor potential canonical 6 (TRPC6) on the proliferation of hUVEC induced by the supematant from human prostate cancer cells (DU145). Methods Experiment group was treated with the mixture of DU145 cells supernatant and DMEM medium in different ratio, control group was treated with DMEM medium. The change of cell proliferation was detected by MTT method, and TRPC6 gene and protein was detected by PCR and Western-blot methods respectively. Results After incubated for 48h with the supematant from cultured DU145 cells,the population of hUVECs increased obviously, and the expression of TRPC6 genes and protein had clearly up-regulation. Conclusion The supernatant from cultured DU145 cells could effectively enhance the proliferation of hUVECs. The mechanisms might be that the proliferation of human umbilical vein endothelial cells was mediated by TRPC6.

Objective To observe the influence of diabetes mellitus or insulin on fluctuations of serum potassium in patients with maintenance hemodialysis.Methods 52 cases of hemodialysis patients,including 30 non-diabetes patients,11 patients with diabetes but not using insulin,11 diabetes patients using insulin.At the time of 0,24,48 h during dialysis,we collected serum samples from the 52 patients,serum potassium was compared among the three groups.Results On 0,24,48 h,serum potassium level of the diabetes but not using insulin group had no significant difference with the non-diabetes group.While in diabetes using insulin group,serum potassium level was lower compared with the non-diabetes group or diabetes not using insulin group.Conclusions Diabetes itself had no effect on serum potassium fluctuations of maintenance hemodialysis (MHD) patients,however,insulin can reduce the pre-dialysis serum potassium levels of MHD patients.