Objective To observe the effect of electroacupuncture on the expression of vascular endothelial growth factor receptor-2 (VEGFR-2) mRNA and brain-derived neurotrophic factor (BDNF) protein in brain tissue of rats with focal cerebral ischemia/reperfusion.Methods The focal cerebral ischemia/reperfusion model was established with filament occlusion. 90 rats were randomly divided into control group (n=6), model group (n=42) and electroacupuncture group (n=42). They were observed 2 h, 12 h, 24 h, 3 d, 7 d, 14 d, 21 d after ischemia/reperfusion, 6 rats in each points. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of VEGFR-2 mRNA. Immunohistology method was used to detect the expression of BDNF protein. Results The expression of VEGFR-2 mRNA was higher in the electroacupuncture group than in the model group 12 h, 24 h, 3 d after reperfusion (P<0.05). The expression of BDNF protein in the hippocampus increased 2 h (P<0.05), reached peak 24 h (P<0.01) and still higher 3 d (P<0.05) in the electroacupuncture group than in the model group after reperfusion. Conclusion Electroacupuncture can upregulate the expression of VEGFR-2 mRNA and BDNF protein in brain tissue of rats with focal cerebral ischemia/reperfusion. Neuro-angiogenic factor is possibly the molecular basis of coordinated interaction of the nervous and vascular system.

Objective To compare iso-osmolar iodixanol and low-osmolar iohexol for the incidence of contrast- induced nephropathy(CIN) in patients with chronic congestive heart failure undergoing coronary interventional therapy. Methods The study included 220 consecutive patients with chronic congestive heart failure and undergoing coronary angiography (CAG) with or without percutaneous coronary intervention (PCI) bewteen Janurary 2015 and May 2016. Study participants were divided into two groups by random digits table:iso-osmolar group (110 patients) and low-osmolar group (110 patients). The patients in iso-osmolar group were given iodixanol, and the patients in low-osmolar group were given iohexol. Serum creatinine (SCr), glomerular filtration rate (GFR) and cystatin C (CysC) were detected before the procedure and on the first, third day after the procedure. Then, the incidence of contrast-induced nephropathy (CIN) in two groups within 72 h of the procedure were observed and compared. Results The levels of SCr, GFR, CysC before operation had no significant differences (P>0.05). The levels of SCr in two groups on the first day after operation were increased, but there was no significant difference between two groups (P>0.05). On the first day after operation, the level of GFR in iso-osmolar group was higher than that in low-osmolar group, the level of CysC in iso-osmolar group was lower than that in low-osmolar group, and there were significant differences (P<0.05). On the third day after operation, the level of GFR in iso-osmolar group was higher than that in low-osmolar group, the level of CysC in iso-osmolar group was lower than that in low-osmolar group, and there were significant differences (P<0.01). The overall incidence of CIN was 20.9%(46/220). The incidence of CIN in low-osmolar group was 29.1%(32/110), in iso-osmolar group was 12.7%(14/110), and there was significant difference (P<0.05). Conclusions In chronic congestive heart failure patients undergoing coronary interventional therapy, the iso-osmolar contrast iodixanol is associated with a lower incidence of CIN compared with low-osmolar iohexol.

Objective To examine the changes of red blood cell levels in the obese and non-obese patients with coronary heart disease (CHD) and its clinical significance. Methods 230 cases of coronary heart disease were selected and divided into the obese group and the nonobese group. Obesity and non-obesity were defined based on the body mass index (BMI I (Y) 28.0kg/m2), or waist-hip ratio (men> 0.9, women> 0.85). In addition, 130 healthy subjects were recruited as controls. The pathological status of coronary lesions was quantitatively analyzed according to the Coronary Vascular Image Segmentation Evaluation Criteria (American Heart Association 1984) and the Gensini scoring system. Results of the changes of both the hemoglobin levels and the red blood cell count in the obese group, the nonobese group with CHD and the control group were compared. Besides, Multivariant Logistic Regression Analysis was applied to assess the correlation between the red blood cells and the coronary artery disease. Results The red blood cell count and the level of hemoglobin in the obese group with CHD was higher than that in the non-obese group with CHD [(4.35 ± 0.55) and (4.13 ± 0.56) 109/L; (136.71 ± 15.87) and (129.96 ± 16.23) g/L, P < 0.05 in both]; the proportion of acute coronary syndrome in the obese group with CHD was higher in the obese group with CHD than that in the non-obese group with CHD (P<0.05); Multivariant logistic regression analysis also showed that the red blood cell count was positively correlated with obesity with CHD.Conclusion The red blood cell count and the level of hemoglobin in the obese group were higher than those in the non-obese group; the increase of red blood cell count and hemoglobin level is one of the independent risk factors for the obese patients with CHD.

Objective To find out the situation of household consumption of iodized salt in Hebei province so as to provide scientific basis for prevention and control of iodine deficiency disorders(IDD). Methods According to the "national iodine deficiency disorders surveillance program (Trial)", the county (city, district) was taken as a unit, township (town) and administrative villages were selected in accordance with the principle of systematic sampling, then households were chosen by random sampling to collect their edible salt in Hebei province from 2007 to 2009. Salt iodine content was detected by direct titration method. Results A total of 48 675, 48 448 and 48 756 salt samples were collected from 2007 to 2009, respectively. The consumption rate of qualified iodized salt from 2007 to 2009 was 91.16%, 91.96% and 96.17%, respectively. There were 24.6%(41/167)and 18.0%(30/167)counties with consumption rate of qualified iodized salt under 90% in 2007 and 2008, respectively. The percentage of counties with consumption rate of qualified iodized salt above 90% was 100.0%(167/167) in 2009. In general there was a significant differences in frequency distribution of consumption rates of qualified iodized salt among the three years(H = 10.778, P ＜ 0.01 ), and the difference was found significant between 2007 and 2009 as well as between 2008 and 2009(all P ＜ 0.05), but was not significant between 2007 and 2008(P ＞ 0.05). Conclusions The consumption rate of qualified iodized salt at household level increases annually from 2007 to 2009. By 2009,the consumption rate of qualified iodized salt in each county is more than 90%, which has reached the national standard stipulated in "the evaluation programs for fulfilling the goal of eliminating IDD at county level".

Administration, Inhalation ; Animals ; Female ; Male ; Pesticides ; analysis ; toxicity ; Rats ; Rats, Wistar

No abstract available.
Myxoma*

Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Chromosome Aberrations ; Chromosomes, Human, Pair 1 ; Chromosomes, Human, Pair 11 ; Female ; Humans ; Infant ; Leukemia, Myeloid, Acute ; drug therapy ; genetics ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; genetics ; Remission Induction

OBJECTIVETo improve the method for monitoring sleep state and respiratory rhythm of SD rats, providing a solution for rats' chewing on the wires, signal loss and instability problems in the animal model of sleep apnea syndrome (SAS).

METHODSWe improved monitoring electrodes of both electrocorticogram (ECoG) and electromyogram (EMG), signal circuit and animal operation.

RESULTSOperation time was shortened and wound exposure time was reduced, which made it easier for postoperative recovery. The ECoG and EMG signals were more stable with sharp image, and signal circuit lines had better conductivity and material durability, achieving continuous monitoring for a long time and high success rate. We could precisely distinguish the sleep wake state and the sleep apnea events in rats according to these signals.

CONCLUSIONThe improved method is more reliable and practical to test the small animal model of SAS, and is more easily to operate and the signals are more stable.

Animals ; Electroencephalography ; methods ; Electromyography ; methods ; Models, Animal ; Monitoring, Physiologic ; methods ; Rats ; Respiration ; Sleep ; Sleep Apnea Syndromes ; diagnosis

AlM: To describe the expression of hypoxia-inducible factor-1α ( HlF-1α) and erythropoietin ( EPO ) in rats' corneal and evaluate its potential effect on corneal neovascularization ( CNV) growth.
METHODS:The young SD rats (3mo) was chosen and randomly divided into 2 groups, which were experimental group and normal control group. CNV model was established by alkali burn, and the length and area of CNV was observed everyday after operation by slit lamp. After that, the expression of HlF-1α and EPO was measured by SABC and RT-PCR methods at 1, 3, 5, 7, and 14d after alkali burn. The data was analyzed by SPSS 20. 0.
RESULTS:The area of CNV was increasing at 1, 3, 5, 7, and 14d after alkali burn, and the peak point appear at 7d. The growth speed was decreased after 14d. SABC method told us that no HlF-1αand very tiny amount EPO was detected at normal rats' corneal. The expression of the two factors increased at 1d after alkali burn in corneal epithelium and endoderm. The results of RT - PCR showed that a few amounts of HlF-1α and EPO mRNA were detected at normal group. The expression of the two factors was increased at 3d after alkali burn, and the peak value was found at 7d, however, it was decreased at 14d. Statistical difference was found at different time (P<0. 05).
CONCLUSlON: HlF- 1α and EPO is closely related to CNV.

OBJECTIVE:To provide reference for improving the level of rational drug use in public hospitals at county level. METHODS:The relevant indicator data of rational drug use in our hospital was analyzed,and the relevant indicator data of the hos-pitals at county level around it was investigated and analyzed,a total of 36 parts of rational drug use management indicator system was established,including administrative management indicators,outpatient management indicators,hospital management indica-tors,and drug procurement management indicators,and it was constantly improved and optimized. Meanwhile,relevant indicator data of rational drug use from 2010 to 2014 was statistically analyzed to evaluate the effect of the effectiveness of the system. RE-SULTS:In the 5 years,the drug revenues accounted for revenue ratio(the ratio of drug)decreased by 10.3%;the use indicators of antibiotics in clinic showed gradual optimization,the proportion of outpatient antibiotic prescriptions decreased from 57.1% to 19.3%,the use rate of inpatient antibiotic decreased from 90.2% to 56.3%,the proportion of type I incision operation preventive use of antibiotics decreased from 94.6% to 28.6%,and the submission rate of microbiological sample of inpatients before they re-ceived the treatment of antibiotics increased from 8.4% to 32.7%;the proportion of essential medicines increased 25.5%,and pro-portion of use amount increased to 24.1%;the application of the system promoted the improvement of rational drug use and phar-macy management level in our hospital,and also helped the hospital successfully passed the national three class comprehensive hos-pital evaluation. CONCLUSIONS:The established rational drug use management indicator system is comprehensive and feasible, and can provide reference for strengthening rational drug use management and medical and health system decision in China.