The main pollution of coastal sea is heavy metal and eutrophication,which resulted in degeneration of its eco-function.Pollution of heavy metal is accompanied with eutrophication in sea water.This article studied on copper absorption capacity of several bacteria strains which were isolated from the surface of macroalga Gracilaria lemaneaformis that was cultivated in copper polluted sea.We got 6 strains of epiphytic bacteria from the seaweed,Ochrobactrum anthropi,Aeromonas salmonicida,Micrococcus lylae,Corynebacterium ulcerans,Pseudoalteromonas spp,Vibrio fluvialis.Attained from the experiment of copper biosorption,A.salmonicida had a maximal biosorption capacity among them,and Vibrio minimal.Based on this trail,A.salmonicida,C.ulcerans and Vibrio were selected for next trails included optimal biosorption time,optimal pH value and effect of pre-disposal by several chemical reagents on biosorption capacity.The results showed:optimal biosorption time was between 40 minutes and 60 minutes,and optical pH value 4～5.Pre-disposal by HCl leaded to lost the absorption capacity of A.salmonicida,but was opposite by NaOH and ethanol.For C.ulcerans and Vibrio,the copper absorption capacities were increased by them.

We here in the paper, not only introduce the development of electricsurgical operation equipment in our country and foreign countries but also represent the characters, principles and usages of several kinds electrosurgical equipment in foreign countries.
Argon ; Catheter Ablation ; instrumentation ; Electrocoagulation ; instrumentation ; Electrosurgery ; instrumentation ; Humans ; Ions

This article gires you a brief introduction about the main functions and general structure of Argon Ion high-frequency combined electrotome wihich can reduce hemorrhage in operation,prevent uncontrollable bloold-oozing,shorten the time of operation and decrease the risk of cross-infection.It also recommends some key technologies in the system,especially the use of a computer to monitor and control the system for the security and reliability of the system.

OBJECTIVESalvia miltorrhiza Bunge (SMB) is a traditional Chinese herb, which is considered to promote blood flow and remove blood stasis. This study examined whether SMB can alleviate injury induced by hypoxia/reoxygenation (H/R) in human kidney proximal tubular cells-2 (HK-2 cells).

METHODSThere were 3 experimental groups: control, H/R injury and SMB-treated H/R injury. H/R injury of HK-2 cells was induced by first covering the cells with and then removing liquid paraffin wax. Different concentrations of compound SMB solution (0.05%, 0.10%, 0.15% or 0.20%) were administered to the SMB-treated H/R injury group before the hypoxic injury. After 4, 12 and 24 hrs of hypoxia and 4, 12, 24 and 48 hrs of reoxygenation, morphologic changes of HK-2 cells were observed under an inverted microscope. Cell viability was measured by the MTT method. Lactate dehydrogenase (LDH) activity in the culture supernatants was assayed using biochemical methods; TNF-alpha levels were determined by radioimmunoassay (RIA).

RESULTSThe number of HK-2 cells was significantly reduced in the H/R injury group after hypoxia, and reached a nadir 24 hrs after hypoxia treatment. Various concentrations of SMB-treated groups showed significantly greater number of HK-2 cells than the H/R injury group. SMB solution (0.10%) produced the best effect. The levels of LDH and TNF-alpha in the H/R injury group were significantly increased, and reached a peak between 24 hrs of hypoxia and 4 hrs of reoxygenation when compared to the control group. Pre-treating with 0.10% SMB resulted in significantly lower levels of LDH and TNF-alpha than in the untreated H/R injury group at various time points of H/R.

CONCLUSIONSSMB has protective effects against H/R injury of HK-2 cells, possibly through inhibition of inflammatory cytokines.

Calcium ; metabolism ; Cell Hypoxia ; Cells, Cultured ; Cytoprotection ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Kidney Tubules, Proximal ; drug effects ; pathology ; L-Lactate Dehydrogenase ; metabolism ; Plant Extracts ; pharmacology ; Salvia miltiorrhiza ; Tumor Necrosis Factor-alpha ; analysis

The mechanisms of human bone marrow mesenchymal stem cells (hBMMSCs)-mediated immunomodulation are still not be completely clarified. In order to investigate the expression of B7-H1 on hBMMSCs and to explore whether B7-H1 mediated signaling pathway (B7-H1/PD-1) involves in the mechanisms of hBMMSCs-mediated immunomodulation, the hBMMSCs were isolated, cultured and identified, the B7-H1 expression on hBMMSCs was detected by flow cytometry, RT-PCR, and Western blot. The inhibitory effect of hBMMSCs on proliferation of T lymphocytes was observed in mixed lymphocyte culture, and then the functional anti-B7-H1 monoclonal antibody (mcAb) was used to block B7-H1, the proliferation of T lymphocytes was detected by using CCK-8. The results indicated that hBMMSCs highly expressed B7-H1 molecule, hBMMSCs effectively inhibited the proliferation of T lymphocytes with a dose-dependent manner, and the inhibitory proliferation of T lymphocytes by hBMMSCs could be partially restored when the anti-B7-H1 mAb was used to block the B7-H1, the inhibitory rate of T lymphocyte proliferation decreased from 64.1% to 38.75%. It is concluded that B7-H1 highly expresses on hBMMSCs, the B7-H1 mediated signaling pathway (B7-H1/PD-1) involves in the mechanisms for hBMMSCs-mediated immunomodulation.
Antigens, CD ; metabolism ; B7-H1 Antigen ; Bone Marrow Cells ; metabolism ; Cell Proliferation ; Humans ; Lymphocyte Activation ; Mesenchymal Stromal Cells ; metabolism ; T-Lymphocytes ; cytology

OBJECTIVETo evaluate the clinical significance of IgH and TCR gamma gene rearrangement in plasma free DNA in patients with non-Hodgkin Lymphoma (NHL).

METHODSPlasma free DNA in 74 patients with NHL were extracted and identified by Globin gene. IgH (FR3A/VLJH), TCR gamma (TVG/TJX) clonal rearrangements were amplified by PCR and compared with results of mononuclear cell DNA and pathological biopsy sample DNA.

RESULTSPlasma free DNAs were successfully obtained from 58 cases (35 B-NHL and 23 T-NHL) of newly diagnostic, refractory and relapsed NHL out of total 74 patients (78.4%), but not found in the rest 16 patients in remission. Of 35 B-NHL cases, 31 showed IgH rearrangement (88.6%), and none with TCR gamma rearrangement; of 23 T-NHL cases, 8 showed TCR gamma rearrangement (34.8%), and 2 with IgH gene rearrangement synchronously. In comparison with the results of IgH and TCR gamma gene rearrangement in biopsy samples in 30 B-NHL cases, 26 cases in plasma free DNA (86.7%) and 24 in biopsy samples (80%) were positive (P > 0.05). In 20 T-NHL patients, 7 cases in plasma cell-free DNA (35%) and 6 cases in biopsy samples (30%) were positive (P >0.05).

CONCLUSIONSTumor-derived DNA could be detected in plasma from underlying cancer patients. For NHL patients, detecting IgH and TCR gamma gene rearrangement in plasma free DNA has the same clinical significance as in biopsy samples.

Adolescent ; Adult ; Aged ; Child ; DNA ; blood ; Female ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Humans ; Immunoglobulin Heavy Chains ; genetics ; Lymphoma, Non-Hodgkin ; blood ; genetics ; Male ; Middle Aged ; Young Adult

Objective To research the location,composition,zoning,flow and combination form of medical system of Model 920 hospital ship, and to provide theoretical support for the design of the hospital ship. Methods The layout of medical system of the hospital ship was constructed based on the theories of ship engineering design,hospital architecture design and naval health service as well as the requirements for height and internal environment of medical system.Results The mode combining multi corridor and single column was used to design 8 operating rooms and accessories in the midship of No.01 deck.Conclusion The multi-corridor single-column combination operating area occupies less ship space resources and the surgical treatment of wounded and sick patients is efficient, which is suitable for the platform of the ship and is worthy of reference for the design of the medical system of the large-scale rescue platform on the sea. [Chinese Medical Equipment Journal,2018,39(5):39-43]

The purpose of the current study was to examine the pharmacokinetic profiles and tissue distribution of clevidipine, an ultra-short-acting calcium antagonist in Beagle dogs and Sprague-Dawley rats, respectively. The pharmacokinetics and biodistribution of its primary metabolite H152/81 were also evaluated. Dogs received intravenous infusion of clevidipine at a dose rate of 17 μg/(kg·min), and rats were given intravenous administration of clevidipine at a dose of 5 mg/kg. Dog plasma and rat tissues were collected and assayed by HPLC-MS/MS. It was found that plasma clevidipine quickly reached the steady state concentration. The terminal half-life was short (16.8 min), pointing out a rapid elimination after the end of the infusion. The total clearance was 5 mL/(min·kg). In comparison, plasma concentration of H152/81 was increased more slowly and was significantly higher than that of clevidipine. After intravenous administration, clevidipine was distributed rapidly into all tissues examined, with the highest concentrations found in the brain, heart and liver. Maximal concentrations of clevidipine were found in most tissues at 10 min post-dosing. However, the proportion of clevidipine distributed in all tissues was quite small (0.042‰) compared to the total administration dose. It was suggested that clevidipine was mainly distributed in blood and it transformed to inactive metabolite rapidly.
Animals ; Calcium Channel Blockers ; pharmacokinetics ; pharmacology ; Dogs ; Dose-Response Relationship, Drug ; Organ Specificity ; drug effects ; Pyridines ; pharmacokinetics ; pharmacology ; Rats

BACKGROUNDHemodynamic evaluation is crucial for the management of patients with pulmonary hypertention. Clinicians often prefer a rapid and non-invasive method. This study aimed to examine the feasibility of transthoracic echocardiography for the measurements of hemodynamic parameters in patients with pulmonary hypertension.

METHODSA prospective single-center study was conducted among 42 patients with pulmonary hypertension caused by different diseases. Transthoracic echocardiography and right-heart catheterization were performed within 24 hours. Pulmonary artery systolic, diastolic and mean pressure (PASP, PADP and PAMP), cardiac output (CO), and pulmonary capillary wedge pressure (PCWP) were measured by both methods. A linear correlation and a Bland-Altman analysis were performed to compare the two groups of hemodynamic parameters.

RESULTSA good correlation was found between invasive and non-invasive measurements for PASP (r = 0.96), PADP (r = 0.85), PAMP (r = 0.88), CO (r = 0.82), and PCWP (r = 0.81). Further agreement analysis done by the Bland-Altman method showed that bias and a 95% confidence interval for PASP, PADP, and CO were clinically acceptable while great discrepancies existed for PAMP and PCWP.

CONCLUSIONSThe non-invasive measurements by PASP, PADP, and CO in patients with pulmonary hypertension correlate well with the invasive determinations. Transthoracic echocardiography (TTE) was inappropriate for estimating PCWP and PAMP.

Adolescent ; Adult ; Cardiac Catheterization ; Cardiac Output ; Echocardiography ; Female ; Hemodynamics ; Humans ; Hypertension, Pulmonary ; physiopathology ; Male ; Middle Aged ; Prospective Studies ; Pulmonary Wedge Pressure

Objective To observe the clinical efficacy and safety of levosimendan injection combined with noninvasive ventilator in the treatment of elderly patients with acute heart failure.Methods One hundred and sixty-two elderly patients with acute heart failure were randomly divided into control group and treatment group with 81 cases per group.Two groups were received the noninvasive ventilator therapy.Control groups was treated with dobutamine hydrochloride injection 5 μg · kg-1 · min-1 with intravenous injection for 24 h.Treatment group was given levosimendan injection 12 μg · kg-1 with intravenous injection for 10 min,then intravenous injection for 24 h with 0.1 μg · kg-1 · min-1 The clinical efficacy,cardiac output (CO),cardiac index (CI) and heart rate (HR) and adverse drug reactions were compared between two groups.Results After treatment,the total effective rates in treatment and control groups were 96.29％ (78/81 cases) and 86.42％ (70/81 cases) with significant difference (P ＜ 0.05).After treatment,the main indexes in treatment and control groups were compared:the CO were (2.33 ±0.33) (1.79 ±0.24)L · min-1;the CI were (3.23 ±0.46),(2.35 ±0.32) L · min-1· m-2;HR were (97.65 ± 13.27),(112.12 ± 10.89) times · min-1,with statistically significant difference (all P ＜ 0.05).Adverse drug reactions in treatment group were based on dizziness,decreased blood pressure and flatulence,which in control group was based on dizziness and flatulence.The incidence of adverse drug reactions in treatment and control groups were 13.58％ and 11.11％,without significant difference (P ＞ 0.05).Conclusion Levosimendan injection combined with noninvasive ventilator has a definitive clinical efficacy in the treatment of elderly patients with acute heart failure,which can significantly reduce the HR,improve the levels of CO and CI,without increasing the incidence of adverse drug reactions.