Objective The clinical characteristics and treatment outcomes of Listeria monocytogenes (LM) meningitis were reviewed. Methods The clinical data of a case of LM meningitis were retrospectively analyzed and a literature review was performed. Results The child initially presenting with fever, headache and vomit was treated with amoxicillin/clavulanic acid and ceftriaxone and the treatment effect was obvious. Cerebrospinal fluid and blood cultures were LM positive. In 17 related arti-cles, a total of 24 children with LM meningitis were reviewed. Among them, 4 patients died and 10 had hydrocephalus. The treatment with either ampicillin alone or in combination with aminoglycoside was effective. Conclusions LM meningitis was rare in immunocompetent children, but has high rates of mortality and sequelae.

Objective:To investigate the feasibility of 3-D computer aided design(CAD)for the framework of a mandibular Kennedy Class Ⅱ,Modification 2 arch.Methods:In terms of the principle of clinical design,the 3-D framework was built with the technique of mixed model of points cloud,curves and surfaces on the reconstructed model of a partially edentulous cast,including the tissue surfaces and polishing surfaces of every component,such as clasp,rest,lingual bar and mesh construction,etc.Results:Preliminarily,a CAD method for 3-D RPD framework design was established and a individualized RPD framework 3D geometric model was set up.Conclusion:CAD for RPD framework design based on the mixed model of points cloud,curves and surfaces is feasible.

Objective: To reconstruct three dimension (3D) model of a rtificial tooth and to establish standardized 3D artificial teeth graphic datab ase. Methods: Artificial teeth were incised with CGI autom atic cross section scanning instrument, then data points were picked up from e ach parallel layer contour of incised tooth, and 3D stereo models of artificia l tooth were retrieved using 3D reconstruction techniques. Results: 3D graphic models of 28 artificial teeth were established.The models may represent the graphic feature of the teeth. Conclusion: The present technic is feasible in the establishment of 3D graphic models of artificial tooth.

Objective:To establish three-dimension (3D) rectangular coordinate and to obtain relevant parameters of artificial tooth morphology for full denture.Methods:An artificial teeth coordinate was defined based on artificial tooth feature points, which were measured using 3D coordinate-measure-machine.Results:The 3D coordinate of 28 artificial teeth was established and the tooth parameters in the coordinate were obtained.Conclusion:The definition of artificial tooth coordinate using mathematic language is a based work of CAD full denture.

One hundred and six patients with hip fracture admitted in hospital from 2011 to 2014 were randomly divided into two groups: 52 patients received celecoxib and parecoxib ( trial group ) and 54 patients received oxycodone hydrochloride and bucinnazine (control group).On d1 and d7 after the surgery, serum levels of C-reactive protein[(26.2 ±5.4) and (10.4 ±2.8) mg/L], tumor necrosis factor-α[(40.6 ±6.1) and (31.8 ±5.3)ng/L], interleukin-6 [(22.3 ±5.7) and (18.3 ±5.2) ng/L] and prostaglandin E2 [(181.1 ± 19.4) and (153.3 ±25.8) ng/L] in trial group were all significantly lower than those in control group [(46.1 ± 14.0) and (22.1 ±5.2)mg/L, (53.2 ±5.3) and (44.6 ±7.4) ng/L, (43.3 ±11.0) and (29.1 ±8.6)ng/L, (354.3 ±105.9) and (251.0 ±37.3)ng/L, all P<005].The length of hospital stay [(11.0 ±2.2) d], rate of lung infections (6/52) and neurological abnormality (3/52) in trial group were significantly lower than those in control group [(15.5 ±1.8) d, 15/54 and 11/54, all P <0.05].The expression of CD4 +CD45 + T cells [(34.9 ±3.7)% vs.(42.5 ±4.3)%] was reduced and CD4 +Foxp3 +T cells [(19.7 ±3.8)% vs.(6.9 ± 1.5)%] was increased at 24 h after surgery in trial group compared with control group( all P<0.05).The results indicates that calecoxib can reduce the inflammatory reaction by promoting CD4 + to Foxp3 + CD4+T cell differentiation and may improve the prognosis of elderly patients with hip fracture.

ObjectiveTo observe the effect of rabbit skeletal muscle stem cells (RSMSCs) modified by adenovirus mediated bone morphogenetic protein-2 (BMP-2) gene ex vivo in combination with demineralized bone matrix (DBM) on repair of longer bone defect in rabbit.MethodsModel of radial bone defects (20 mm) of rabbits was established. 50 rabbits were divided into 5 groups, group A (AdrhBMP-2 trusduced RSMSCs/DBM group), group B (adGFP trusduced RSMSCs/DBM group), group C (not trusduced RSMSCs/DBM group), group D (DBM group), and group E (untreated group). Roentgenographic, histologic, biomechanical, bone density of all animals were examined at the end of 4th and 6th week after surgery.ResultsAt 4th week, radial bone defects healed in group A. The healing rates from group A to group E were 100%, 50%, 33%, 0%, and 0% respectively at 6th week.ConclusionRSMSCs modified by AdrhBMP-2 ex vivo in combination with DBM can repair radial longer segemental defect in rabbit. It's possible to be used in the clinical treatment of longer segemental bone defect.

Objective: To identify the most appropriate meteorological variable for forecasting the health risk of high temperatures. Methods: The surveillance on causes of death, meteorological data and surveillance on air quality among registered residents in Ningbo City, Zhejiang Province during the period between May and October from 2013 to 2019 were collected. The association models of daily minimum temperature, average daily temperature, daily maximum temperature, daily minimum heat index, average daily heat index, daily maximum heat index, average daily apparent temperature and torridity index with deaths and years of life lost (YLL) were created using time series analysis and distributed lag non-linear models, and the model fitting effect was evaluated using the minimum Akaike information criterion (AIC) procedure. The most appropriate meteorological variable for forecasting gender-, age- and mortality-specific health risks of high temperatures was identified. Results: A total of 120 628 deaths were reported during the study period, with daily deaths of 94 cases, and daily YLL rate of 19.74 person-years/105. Except for daily minimum heat index and torridity index, the exposure-response relationships between other six meteorological variables and deaths and overall YLL rate all appeared a “J” shape. The lowest AIC values and the optimal model fitting effects were measured for the association models between average daily temperature and whole populations, females, subjects at ages of 65 years and older, and deaths and YLL rates due to circulatory diseases and respiratory diseases. Conclusion High model fitting effects are observed between average daily temperature and deaths and YLL rates, which are more suitable for forecasting the health risk of high temperature.

Objective Based on active monitoring MRSA carriage for hospitalized patients, the relationship between colonization pressure and MRSA cross transmission in wards without rigorous contactisolation measures was analyzed, and the role of colonization pressure in predicting MRSA cross transmission was further evaluated. Methods From March to December 2009, active MRSA colonization screening was performed for 240 hospitalized patients in emergency ward and 94 cases in RICU in our hospital. rep-PCR method was employed to do homology analysis on MRSA strains obtained in this study. MRSA weekly colonization pressure, threshold colonization pressure ,cross transmission rate were calculated respectively. RR of MRSA cross transmission under higher level of colonization pressure and lower level of colonization pressure was analyzed. Results MRSA carriage rates on admission for patients in emergency wards and RICU were 6. 25% (15/2A0) and 13. 83 % (13/94) ,and MRSA cross transmission occurred in 13 weeks and 14 weeks in above two units, respectively. Threshold colonization pressures for above two units were 6. 49%and 17. 66%, respectively. For emergency ward, the MRSA cross transmission rate under higher level of colonization pressure was significantly higher than that under lower level of colonization pressure (x2 = 7. 10,P＜0. 01), the RR of MRSA transmission was 9. 61 (95% CI:1. 25-74.00). For RICU, the MRSA cross transmission rate under higher level of colonization pressure was significantly higher than that under lower level of colonization pressure(x2 = 12. 60, P＜0. 01 ), the RR of MRSA transmission was 15.87 (95% CI:2. 06-122. 10). Conclusions Higher level of colonization pressure is an important risk factor for MRSA transmission, and average colonization pressure can be used as a prediction index for MRSA transmission and strengthening prevention and control measures.

AIM: To observe the curative efficacy of Shenkang Bolt(SKB) in treating chronic renal failure(CRF) with blood silt and damp pathogen syndrome. METHODS: The randomized,parallel and controlled trial were used.38 cases in treatment group were treated with SKB,and 40 cases in control group were clystered with Chinese herbal medicine.The changes of the cumulative scores of traditional Chinese medicine(TCM) syndrome,the levels of serum creatinine(SCr) and creatinine clearance rate(CCr) before and after treatment in both groups were observed. RESULTS: The total effective rate was 81.5% in the treatment group,but 75% in the control group.The scores of TCM syndrome and the levels of SCr were both significantly decreased(P(0.05)). CONCLUSION: SKB can markedly improve the clinical manifestations of CRF with blood silt and damp pathogen syndrome,decrease the levels of SCr,but increase CCr.There was no difference between Chinese herbal clysis and Shenkang Bolt in curative effect,but it is convenent to take Shenkang Bolt.

Objective To investigate the diagnosis and application value of Color Doppler Flow Imaging(CDFI)and Transcranial Doppler(TCD)on blood hemodynamics of carotid artery and cerebral arteries in patients with type 2 diabetes mellitus.Methods Sixty-seven patients with type 2 diabetes mellitus and 53 normal subjects were examined on blood hemodynamics of carotid artery and cerebral arteries by CDFI and TCD,and their ultrasonographic features were recorded and compared.Results Forty-six of 67 patients have atherosclerosis on carotid artery by CDFI,56 of 67 patients have abnormal on cerebral arteries by TCD,there were significant difference between the patients and normal groups(P