Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; CD4 Antigens ; metabolism ; CD56 Antigen ; metabolism ; Cyclophosphamide ; therapeutic use ; Dendritic Cells ; pathology ; Diagnosis, Differential ; Doxorubicin ; therapeutic use ; Female ; Humans ; Leukemia, Myeloid ; metabolism ; pathology ; Lymphoma, Extranodal NK-T-Cell ; metabolism ; pathology ; Plasmacytoma ; drug therapy ; metabolism ; pathology ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; pathology ; Prednisone ; therapeutic use ; Skin Neoplasms ; drug therapy ; metabolism ; pathology ; Vincristine ; therapeutic use

Objective To compare the clinical features of space infections in the oral maxillofacial region between patients with diabetes mellitus and patients without diabetes.Methods A retrospective study was conducted on 61 patients diagnosed with space infections in the oral maxillofacial region and receiving treatment at the department of oral and The 4nd Affiliated Hospital of Kunming Medical University between 2013 and 2016.The patients were divided into 2 groups:diabetic group (n =28) and non-diabetic group (n =33).The study variables were demography,etiology,clinical and laboratory examinations (bacteriology,admission blood glucose level,admission white blood cell count and percent of neutrophil on admission).The outcome was compared.The length of hospital stay and complications were also compared.Statistical analyses of the results between groups were performed using the t test and chi square test.Results For oral and maxillofacial space infection,there was no statistically significant difference in gender between the diabetic group and (28 cases) and non diabetes group (33 cases) (x 2 test,P=0.5929);while there were statistically significant differences between two groups in age (t test,P＜0.001) and in the number of infection space (t test,P＜0.001);there was no significant difference between the two groups in the sources of infection (x 2,P＞0.05);The main source of infection was odontogenic,followed by adenogenous.There were statistically significant differences between two groups in blood glucose concentration at admission (t test,P＜0.001) and in the hospitalization time and complications (t test and x 2 test P＜0.05),while no statistically significant differences in the white blood cells count and neutrophil count (t test,P＞0.05).Compared with the non diabetic group,for patients in the diabetic group,the age of onset was older,the involvd gaps were more,the blood glucose concentration was higher on admission,the length of stay was longer,complications were more likely to occur,and the consequences were more serious.Conclusion Compared with non diabetes group,space infection in oral and maxillofacial region is more serious and the hospital stay is longer and has more complications in diabetic patients which should be paid higher attention to.

Objective To review the experience of aortic root internal drainage in the operation of type A aortic dissection.Methods This was a prospective and observational study.Clinical data were available from 20 cases of type A aortic dissection patients (A group) from March 2003 to March 2008,and anothcr 36 cases of type A aortic dissection patients (B group) from March 2008 to May 2013.All of patients received the operation of replacement of ascending aortic aneurysm and aortic arch replacement and descending aortic stented elephant trunk implantation in our hospital.The additional inside drainage were made between the aortic root aneurysm sac and right atrial appendage in B group.Perioperative and postoperative data including the operation time,the amount of bleeding,cardiopulmonary bypass time,the amount of red cell transfusion,number of postoperative cases re-operated for hemostasis,total drainage volume in 24 hours,amount of postoperative red blood cell transfusion,number of cases of postoperative pulmonary complications,and postoperative mortality rate were analyzed retrospectively.The data was analyzed using SPSS version10.0 software.The chi-square test was used for constituent ratios,whilest was applied to analysis of differences in above variables betweens two groups.Results There were significant differences in the amount of bleeding during surgery,cardiopulmonary bypass time,the amount of red cell transfusion,rate of postoperative re-operation,total drainage volume in 24 hours,amount of postoperative red blood cell transfusion,number of cases of postoperative pulmonary complications and postoperative mortality between the two groups (x2/t =2.658,2.381,2.265,3.056,6.862,2.896,2.316,7.215,7.668,P =0.012,0.034,0.007,0.016,0.013,0.032,0.008,0.008,respectively).Conclusions The method of aortic root internal drainage in the operation of type A aortic dissection could reduce the amount of bleeding and improve the success rate of operation.

Objective To investigate surgical techniques and results of arthroscopic reduction and fixation for the treatment of avulsion fracture of the posterior cruciate ligament from the tibia through an additional posterolateral portal. Methods An additional posterolateral portal was established by using the inside-out technique under arthroscopic view.After a protective sleeve was placed through the portal and the displaced fracture was reduced,the fragment was fixed by the guide wire drilled through the sleeve temporarily.If the fluoroscopic control showed an anatomic reduction of the fragment and good placement of the wire,the self-attacked cannulated screw and washer was placed intra-articularly over the guide wire through the sleeve for the directive internal fixation of the avulsion fracture.Aggressive rehabilitation programs were recommended postoperatively.Results Except 1 case with 11 mm displacement and rotation of the fragment treated by the arthrotomy after the failure of arthroscopic reduction,the arthroscopic operation was accomplished in all the remaining 10 cases.The operative time was 63～98 min(mean,87.3 min).No injuries of popliteal vessels or nerves occurred.The postoperative X-ray firms showed an anatomic reduction of the fragment and good placement of the screw and washer.With the negative posterior drawer test confirmed by the physical examination,normal range of motion of the injured knee joint and gait were achieved in the 10 patients at 4～7 weeks after surgery.The bone union was confirmed by X-ray films at the 3 months postoperatively.The computer KT-2000 arthrometer measurements of posterior tibial displacement of both knee joints showed side-to-side difference not more than 1.2 mm in 6 patients at 5 months after surgery.Conclusions The additional posterolateral portal can be established by the arthroscopic inside-out technique safely.The displaced fragment of the avulsion fracture of the posterior cruciate ligament from the tibia can be reduced and fixed with the self-attacked cannulated screw and washer arthroscopicly.

Objective To investigate the function of donor-derived dendritic cells (DCs) treated with NF-?B decoy in prolonging murine cardiac allograft survival time.Methods Donor bone marrow- derived DCs were treated with NF-?B decoy in vitro.Heterotopic abdominal heart transplantation was performed from BALB/c to C57BL/6 mice.Recipients were grouped according to different pretreat ments as follows:(1) Control group,infusion of PBS (0.2 ml) alone intravenously via the portal vein 7 days before heart transplantation;(2) CsA group,treated with sub-therapeutic CsA only for 7 days (10 mg?kg~(-1)?day~(-1)) through intraperitoneal injection after transplantation,and the same as control group before transplantation;(3) Control DCs group,infusion of only cultured 5th-day recipient DCs untreated with NF-?B ODN decoy;(4) Treated DCs group,infusion of recipient DCs pretreated with NF-~cB ODN decoy;(5) Combined treatment group,infusion of recipient DCs treated with NF-~cB ODN decoy before transplantation and intraperitoneal injection of sub-therapeutic CsA for 7 days (10 mg?kg~(-1)?day~(-1)) after transplantation;(6) Third party donor group,C3H/HeJ mice used as donor, and recipient (C57BL/6) was treated the same as combined treatment group.Every group had 8 mice and graft survival time was observed.Cytokines (IL-2,INF-?,IL-4 and IL-10) in recipient serums were analyzed by ELISA at 7th day after transplantation.Results The graft mean survival time (MST) in control group,CsA group,Control DCs group,treated DCs group,combined treatment group and third party donor group was 7 days,10.3 days,7.6 days,21.4 days,53.6 days and 9 days,respectively.There was significant difference in MST between treated DCs group and control group or control DCs group (P

BACKGROUND: Myogenic regulatory factors Myf-5 is an importance gene involved in muscle occurrence process to adjust and control, start and maintain the skeleton muscle cell growth. It has a possible relation with denervated skeletal muscle atrophy. OBJECTIVE: To detect the expression of Myf-5 gene in different skeletal muscle sitas and different periods following denervatad skeletal muscle atrophy.DESIGN, TIME AND SE'I-rlNG: Randomized control animal experiment was performed in the Shanxi Medical University between March and April in 2008. MATERIALS: A total of 24 Sprague-Dudley male rats was selected and divided randomly into four groups, with six in each, Le. sham operated group (innervations), denervated 2 d group, denarvated 7 d group and denervatad 28 d group. METHODS: Sham operations were made only for the rats in the sham operated group and sciatic nerve were not cut off. Sciatic nerves were cut off more than one centimeter at the mid-level of their right lower limb for the rats in denervated groups. The rats were executed by vertebrae dislocation method at 2, 7, 28 days after denervation. The skeletal muscles of the right lower limb (tibialis anterior, soleus, gastrocnemius and plantar) were dissected and dissociated. MAIN OUTCOME MEASURES: Myf-5 mRNA expression was detected by reverse transcription-polymerase chain reaction. Immunohistochemical stain (ABC method) with polyclonal antibody against Myf-5 protein was performed and the gray value was counted.RESULTS: Expressions of Myf-5 mRNA in denervatad skeletal muscle were up-regulated at 2, 7, 28 days in the eady stage of denervation (P < 0.05). The number of Myf-5 antibody positive-stained cell nuclei was the most in satellite cells at 28 days following denervation. CONCLUSION: At the early stage of denervated skeletal muscle atrophy in SD rats, expressions of Myf-5 in different skeletal muscles are all up-regulated. Denervation of rat skeletal muscle raises the Myf-5 expression in satellites cells.

BACKGROUND: MyoD expression of skeletal muscle of rats increases distinctly in the earlier period of denervation, which can greatly postpone denervated skeletal muscle atrophy. The clinical test testifies that electrical stimulation is an effective method to cure denervated muscle atrophy. But the influence of electrical stimulation on MyoD expression during denervated muscle atrophy is still unproved. OBJECTIVE: To discuss the influences of electrical stimulation on the gene expression of MyoD of skeletal muscle of rats. DESIGN, TIME AND SETTING: Randomized control animal experiment was performed in the Animal Experimental Center of Shanxi Medical University between July and November in 2008. MATERIALS: A total of 36 healthy Sprague-Duwley rats of either gender were divided randomly into three groups, control group, denervation group and electrical stimulation group. Each group contained 12 rats. METHODS: Standard models of right sciatic nerve dissociation and gastrocnemius denervation were established in right limb of each rat in denervation group and electrical stimulation group. Thirty-minute electrical stimulation was given to the denervated gastrocnemius muscle of each rat of electrical stimulation group once a day. The rats were executed at 2, 7, 14, 28 days after denervation to dissect and dissociate their gastrocnemius muscles of the right lower limbs. MAIN OUTCOME MEASURES: MyoD mRNA expression was detected by reverse transcription polymerase chain reaction, and MyoD protein level by immunohistochemistry. RESULTS: The expression of MyoD mRNA and MyoD protein level in specimens of denervation group and electrical stimulation group were up-regulated at 2, 7, 14, 28 days after denervation, with significant differences compared with blank control group (P

Purpose:To investigate the clinical feature of small-cell esophageal carcinoma,and try to find rational combined therapy modality.Methods:A retrospective study was conducted in a total of 11 cases of small cell esophageal carcinoma hospitalized from 1990 to 1999 and the literature of small cell esophageal carcinoma in recent years were re- viewed.Results:The median survival time of these patients is 11 months,five cases survived over 1 year,the 1 yr survival rate is 45.45%;only one patient survived over 3 years;nine cases received multimodality treatment,the median survival time was 12 months,and of which,the median survival time of the seven patients treated with combined regimens including surgical operation was 15 months,and the survival time was 10 months and 7 months respectively in two patients treated with combined regimens not including surgical operation.one patient underwent surgery alone,the survival time was 5 months;one patient received chemotherapy alone and survived 6 months.Conclusions:Small cell esophageal carcinoma has a poor prognosis.multimodality treatment including chemotherapy is an effective approach in improving survival;surgical operation play an important role which can not be replaced and preoperative chemotherapy combined with surgical resection and postoperative chemotherapy may be a better combined treatment modality in local regional small cell carcinoma of e- sophagus.

Objective To evaluate the efficacy and safety of composite milk clotting enzyme capsules in children with functional dyspepsia.Methods A multicenter,randomized,open-label clinical trial was carried out in 4Guangzhou hospitals during Feb.2012 to Mar.2013.Children with functional dyspepsia on basis of Rome Ⅲ criteria were enrolled and treated with composite milk clotting enzyme capsules.Total symptom score and drug-related adverse reactions were evaluated after one-week and two-week therapy.The treatment outcome were divided into very effective,fairly effective and ineffective according to the symptom score changes.Superiority test was done for effectiveness of the treatment based on efficacy difference by 10％ regarded as superiority.Results Two hundred and one children were enrolled.One hundred and ninety-six children completed the study as planned.After two-week therapy,better effective rate and total effective rate were 68.88％ and 87.76％,higher than those of one-week therapy (27.04％,76.02％) (U =2.935,P ＜ 0.05).The lower limits of 95 ％ confidence interval of difference in symptoms improve rate,better efficacy rate and symptom score decrease rate between two-week and one-week therapy were more than 10％,and two-week therapy was superior to one-week therapy.No significant drug-related adverse reaction was found during the study.Conclusions Composite milk clotting enzyme capsules can improve functional dyspepsia symptoms significantly.The effectiveness of two-week of treatment is better than that of one-week treatment.No obvious drug-related adverse reaction is observed.

Objective To evaluate the therapeutic efficacy of intratympanic dexamethasone injections combined with prednisone in patients with idiopathic sudden sensorineural hearing loss. Methods A total of 71 patients diagnosed with sudden hearing loss were treated with intratympanic dexamethasone injections plus prednisone (B group) or prednisone alone (A group). Hearing was evaluated by pure tone audiogram performed before initial treatment and at 4 weeks following the final treatment. Results The total recovery rate after the treatment was 81.8% in the B group and 55.3% in the A group. The diflference between two groups was statistically significant (P < 0.05). Conclusion The present study suggests that sudden sensorineural hearing loss patients treated with intratympanic dexamethasone combined with prednisone have a higher likelihood of hearing recovery than those treated with prednisone alone.