1.The expression and binding property of melatonin receptor in adult hepatoma tissue
Xiaobin KANG ; Yong MA ; Wei WEI
Chinese Pharmacological Bulletin 2010;26(2):174-177
Aim To determine whether there existed melatonin receptor (MR) in adult hepatoma tissue and to observe its property of binding.Methods Immuno-histochemistry was used to detect MR and identify its subcellular distribution. Specific binding and kinetic analyses of melatonin receptor were measured by radioligand binding assay.Results The results of immunohistochemistry showed that MT1 and MT2 existed in membrane, cytosol and nucleus of the hepatoma cells, but the expression of them principally localized in the membrane and cytosol. The specific binding assay properties of MR were presented as follows: the maximum binding capacity (B_(max)) was (0.29±0.07) pmol·g~(-1) protein. Equilibrium dissociation constant (K_d) was (48.7±6.5) pmol·L~(-1). The trait of MR, saturation and reversibility, was detected by ~(125)I-Mel specific binding kinetic analyses.Conclusions MR exists in adult hepatoma cells, furthermore, the subtypes of MR (MT1 and MT2) coexist in the membrane and cytosol, respectively,whose characters of the specific binding sites were low binding capacity,high affinity,saturation and reversibility.
2.A Clinico-Epidemilological Study of 55 Cases of Chidhood Idiopathic Thrombocytopenic Purpura.
Won Ho KANG ; Myung Hee KOOK ; Yong Sang YOO ; Jae Suk MA ; Tai Ju HWANG
Journal of the Korean Pediatric Society 1987;30(11):1207-1212
No abstract available.
Purpura, Thrombocytopenic, Idiopathic*
3.Effect of Acutely Increased Glucose Uptake on Insurin Sensitivity in Rats.
Yong Woon KIM ; Youl In MA ; Suck Kang LEE
Yeungnam University Journal of Medicine 1997;14(1):53-66
Insulin resistance is a prominent feature of diabetic state and has heterogeneous nature. However, the pathogenetic sequence of events leading to the emergence of the defect in insulin action remains controversial. It is well-known that prolonged hyperglycemia and hyperinsulinemia are one of the causes of development of insulin resistance, but both hyperglycemia and hyperinsulinemia stimulate glucose uptake in peripheral tissue. Therefore, it is hypothesized that insulin resistance may be generated by a kind of protective mechanism preventing cellular hypertrophy. In this study, to evaluate whether the acutely increased glucose uptake inhibits further glucose transport stimulated by insulin, insulin sensitivity was measured after preloaded glucose infusion for 2 hours at various conditions in rats. And also, to evaluate the mechanism of decreased insulin sensitivity, insulin receptor binding affinity and glucose transporter 4 (GLUT4) protein of plasma membrane of gastrocnemius muscle were assayed after hyperinsulinemic euglycemic clamp studies. Experimental animals were divided into five groups according to conditions of preloaded glucose infusion: group I, basal insulin (14+/-1.9 micronU/ml) and basal glucose (75+/-0.7 mg/dl), by normal saline infusion; group II, normal insulin (33+/-3.8 micronU/ml) and hyperglycemia (207+/-6.3 mg/dl), by somatostatin and glucose infusion; group III, hyperinsulinemia (134+/-34.8 micronU/ml) and hyperglycemia (204+/-4.6 mg/dl), by glucose infusion; IV, supramaximal insulin (100+/-2.2 mg/dl), by insulin and glucose infusion; group V, supramaximal insulin(4813+/-687.9 micronU/ml) and hyperglycemia (233+/-3.1 mg/dl), by insulin and glucose infusion. Insulin sensitivity was assessed with hyperinsulinemic euglycemic clamp technique. The amounts of preloaded glucose infusion(gm/kg) were 1.88+/-0.151 in group II, 2.69+/-0.239 in group III, 3.54+/-0.198 in groupIV, and 4.32+/-0.621 in group V. Disappearance rates of glucose (Rd, mg/kg/min) at steady state of hyperinsulinemic euglycemic clamp studies were 16.9+/-3.88 in group I, 13.5+/-1.05 in group II, 11.2+/-1.17 in group III, 13.2+/-2.05 in group IV, and 10.4+/-1.01 in group V. A negative correlation was observed between amount of preloaded glucose and Rd )r=-0.701, p<0.001) when all studies were combined. Insulin receptor binding affinity and content of GLUT4 were not significantly different in all experimental groups. These results suggest that increased glucose uptake may inhibit further glucose transport and lead to decreased insulin sensitivity.
Animals
4.Effect of Unilateral Pneumonectomy on the Compensatory Growing Pattern and the Function of the Type II Pneumocyte of the Contralateral Lung in Adult Rabbits.
Il Youl MA ; Jeong Ok HAH ; Yong Man LEE ; Suck Kang LEE
Journal of the Korean Pediatric Society 1988;31(10):1315-1320
No abstract available.
Adult*
;
Humans
;
Lung*
;
Pneumocytes*
;
Pneumonectomy*
;
Rabbits*
5.Association of viscera1 fat area measured by bioe1ectrica1 impedance ana1ysis with sex, age and ;metabo1ic risK factors
Fan YANG ; Kang LI ; Ying YANG ; Yan ZHANG ; Yanjun GONG ; Wei MA ; Jie JIANG ; Yong HUO
Chinese Journal of Interventional Cardiology 2016;24(3):134-141
Objective To investigate the differences in visceral fat area measured by bioelectrical impedance analysis in different sex and age groups, and explore the relationship between visceral fat area and other metabolic risk factors. Methods This study enrolled 72 in-patients in the department of cardiology in Peking University First Hospital between August, 2014 and October, 2014. The visceral fat area and the subcutaneous fat area were measured by DUALSCAN HDS-2000 in all patients. Results were compared between different sex and age groups and the relationship between visceral fat area and metabolic risk factors were analyzed. Resu1ts Male had larger visceral fat area than female [ ( 114. 04 ± 38. 27 ) cm2 vs. (92. 09 ±30. 57)cm2, P=0. 019], while female had larger subcutaneous fat areas than male [(223. 92 ± 73. 58)cm2 vs. (270. 35 ± 82. 13) cm2, P =0. 019] . Subcutaneous fat area and visceral fat area were positively correlated in both male ( r=0. 777, P﹤0. 001) and female ( r=0. 601, P=0. 002). There were no significant differences in visceral fat area among different age groups (P=0. 582). And visceral fat area had a positive correlation with body mass index (r=0. 748, P﹤0. 001), waist-hip ratio (r=0. 577, P﹤0. 001), abdominal circumference (r =0. 752, P ﹤0. 001) and HbA1c levels (r =0. 413, P =0. 001). Conc1usions There are sex differences in visceral fat area and subcutaneous fat area. The visceral fat area max be related to blood glucose levels and presence of diabetes.
6.Trabeculectomy punch combined with a novel RS tube for the treatment of punctal stenosis
Yong-Zhe, TANG ; Hong-Ling, LYU ; Hai-Zhi, MA ; Xiao-Xia, LIU ; Kang-Fu, LIANG
International Eye Science 2017;17(7):1365-1367
AIM: To investigate the feasibility and clinical effect of punctoplasty by using trabeculectomy punch combined with a novel RS tube for the treatment of punctal stenosis.METHODS: Totally 39 patients (39 eyes) with punctual stenosis were selected from October 2013 to October 2015 in the Second People`s Hospital of Foshan.All patients underwent punctoplasty by using trabeculectomy punch combined with a novel RS tube.These tubes were removed at 3mo after operation.A follow-up of 6mo was taken for final analysis.The fluorescein dye disappearance test score was recorded before the operation and at 1,3 and 6mo after the extubation.The curative effect of the operation at 6mo after the extubation was assess.RESULTS: Fluorescein dye disappearance test: the scores at 1,3 and 6mo after the extubation all decreased compared with the preoperative ones.The difference was statistically significant(P<0.05).At the last following up, 35 eyes (90%) were cured completely, 4 eyes (10%) were improved significantly, no patients recurred.Effective rate was 100%.No serious intraoperative and postoperative complications happened.CONCLUSION: Punctoplasty by using trabeculectomy punch combined with novel RS tubes is a safe and effective method for the punctul stenosis, which is easy to perform, with high success rate.
7.Clinical Observation on Acute Rheumatic Fever in Children.
Yong Sang YOO ; Won Ho KANG ; Jae Sook MA ; Tai Ju HWANG ; Ki Young LEE ; Sang Woo KIM
Journal of the Korean Pediatric Society 1986;29(4):20-26
No abstract available.
Child*
;
Humans
;
Rheumatic Fever*
8.A Case of Term Cesarean Delivery in Patient with Ascending Aortic Aneurysm : Complicated by Rupture of Aortic Dissection.
Jae Nam MA ; Tae In LEE ; Sun Hee KO ; Kang Woo JUNG ; Jong Cheon WEON ; Yong Pil KIM
Korean Journal of Perinatology 2001;12(1):54-57
No abstract available.
Aortic Aneurysm*
;
Humans
;
Rupture*
9.Clinical Analysis of 149 Cases of Twin Pregnancies.
Yeoung Chan PARK ; Sun Hee KO ; Tae In LEE ; Jae Nam MA ; Kang Woo JUNG ; Jong Cheon WEON ; Yong Pil KIM
Korean Journal of Obstetrics and Gynecology 2000;43(9):1539-1543
No abstract available.
Humans
;
Pregnancy, Twin*