Adenosine Triphosphate ; Mouth ; metabolism ; Receptors, Purinergic P2X ; metabolism

Child ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; surgery

miRNA is a kind of endogenous non-coding short RNA. Mature miRNA was formed through the process of shearing and transporting after genetic transcription. miRNA exhibits many important biological functions through regulating expression and translation of target mRNAs. Different miRNAs may act as oncogenes or antioncogenes, and have tissue specificity. The progress of the tumorigenesis is usually accompanied by expression-profile changes of miRNAs. MiRNA regulates many tumor biological behaviors such as differentiation, proliferation, apoptosis, invasion, metastasis, and drug resistance of tumors. Furthermore, some miRNAs have clinical significance in predicting prognosis of tumor patients.

BACKGROUND: It is thought in traditional Chinese medicine that "the head is the residence of intelligence", "the head is the leader of body, and the focus of the spirit". Therefore, scalp electro-acupuncture can induce resuscitation, relieve mental confusion, dredge the meridian and eliminate the depression in treatment of post-stroke depression.OBJECTIVE: To observe the curative effect of head acupuncture on poststroke depression.DESIGN: Randomized controlled and single blind evaluationSETTING: Department of Acupuncture and Moxibustion, Nanfang Hospital of Southern Medical University; College of Acupuncture and Massage,Guangzhou University of Traditional Chinese Medicine.PARTICIPANTS: Totally 90 patients with post-stroke depression were from Affiliated Nanfang Hospital and,Zhujiang Hospital of Southern Medical University, First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine were recruited. The Score of all the patients in Hamilton Rating Scale for Depression (HRSD) were ＞ 20. Ninety patients were randomly divided as scalp acupuncture group (n=46) and routine acupuncture group (n=44).METHODS: The patients in the routine acupuncture group were treated mainly at points of three-yang meridians of foot and hand at the hemiplegia side. The points include jianyu (LI-15) , binao (LI-14) , quchi (LI-11),shousanli (LI-10), waiguan (SJ-5), hegu (LI-4), biguan (St-31), liangqiu (St-34), zusanli (St-36), yanglingquan (GB-34), kunlun (UB-60), jiexi (St41). The above points were divided into 2 groups, 1 group each day, alternately. Even reinforcing-reducing method was used, with 30-minute needle retention, 6 successive days treatment with 1 day rest, for 6 weeks totally.The patients in the scalp acupuncture were treated with, besides the above method, scalp acupuncture. Mid-parietal line, mid-frontal line, laterofrontal lineI (both sides)were chosen. After arrival of qi, G6805 type electro-acupuncture apparatus (interrupted wave, 50 Hz,4 V) was connected, with 30-minute needle retention, 6 suocessive days treatment with 1 day rest, 6 for 6 weeks totally. HRSD was used to evaluate the status of depression before and after treatment. Stroke score was used to evaluate the curative effect of stroke.MAIN OUTCOME MEASURES: HRSD scores and curative effect of apoplexy before and after treatment of the patients in two groups.RESULTS: According to the intention treatment, all 90 patients entered the result analysis. ① HRSD score after treatment was lower than that be fore treatment in the scalp acupuncture group,and that after treatment group in the routine acupuncture group (8.55±1.73,30.61±4.02,15.04±2.33,P ＜ 0.01 ). ②There was no significant difference of the total effective rate in the two groups , but some tendency indicated the effective rate in the scalp acupuncture group was better than that in the routine acupuncture group ( 100% ,98% ,x2=1.9167 ,P ＞ 0.05).CONCLUSION: Scalp electro-acupuncture has obvious curative effect on post-stroke depression. The amelioration of melancholia has a certain promoting effect on rehabilitation of apoplexy.

Objective To investigate the changes of T cells and disease development in the mice with Smad3 deficiency in the bone marrow cells.Methods Bone marrow cells obtained from Smad3 null(Smad3~(-/-))mice and wild type(Smad3~(+/+))mice were injected to Co~(60)-irradiated GFP mice,respectively.The general states of the bone marrow recipients were observed.The mice were sacrificed at the sixth week and the histopathological changes in the intestines were examined.The changes of T cells from lymph nodes were detected by flow cytometry.Results Smad3~(-/-) bone marrow recipient mice appeared a wasting syndrome and intestinal inflammation.The amount of CD4~+ CD62L~(lo) T cells in lymph nodes was significantly increased.Conclusion These results indicate that the mice with Smad3 deficiency in the bone marrow cells present an inflammatory disorder and their T cells are activated.

Objective To analyze the clinical and pathologieal characteristics of IgA nephmlogy secondary to primary sj(o)gren's syndrome(pSS).Method The clinical and pathological data of 8 patients with pSS and IgA in Changzheng Hospital from 2004 to 2009 were analyzed.Results The average age of seven female and one male was (44±8) years old.Five patients presented with edema and proteinuria.Eight patients presented with microhematuria.The average 24-hour proteinuria was (3±4) g.Two patients had hypertension.Serum creatinine levels of two patients were higher than normal level,the others'were normal.Light microscopy examination showed three patients were mild mesangial proliferation with Lee's classification grade Ⅰ;five patients had global sclerosis with Lee's classification grade Ⅱ,Ⅲ and Ⅳ.Positive IgA was mainly found under immunofluorescence microscopy.Electronic microscopy showed no electron-dense deposits.Conclusion IgA nephrology secondary to pSS is different from primary IgA nephrology under immunofluorescence microscopy.

Objective To improve the descriptions of health information network resources by analyzing the influen-cing factors of information retrieval.Methods The top 10 papers retrieved on 12 Websites in Chinese and English engines according their titles, keywords and descriptions in the HEAD file of HTML source code with 5 biomedical hot words as the search terms were comparatively analyzed and scored.Results The score of Websites in Chinese and English engines was higher than that of medical and health information network, the score of Chinese network information resources was higher than that of English network information resources .Conclusion The current de-scription of network health information resources is poor .Only by strengthening health information network con-struction and improving the description of health information resources , can more accurate health information be provided for the users.

SCI-E and SSCI covered papers on psychiatry published from 2002 to 2011 by authors of China, USA and Japan were analyzed using bibliometrics and the number, relative impact power and international cooperation of these papers were compared among the 3 countries, which showed that both the number and impact power of SCI-E and SSCI covered papers on psychiatry published by authors of China increased, the number of papers published by authors of China was catching up with that published by authors of Japan, and the impact power of papers published by authors of China was higher than that published by authors of Japan.However, they were significantly lower than those published by authors of USA.It is quite important to publish papers in journals with a high academic level and to strengthen international cooperation for increasing the domestic scientific research level in psychiatry.

Objective To explore the emotional socialization development between boys and girls aged 1-3 years. Methods A total of 1008 children were randomly selected with 571 boys and 437 girls. Chinese version of Urban Infant-Toddler Social and Emotional Assess-ment (CITSEA) was used to assess the emotional socialization development situation between boys and girls. Results There were statistical-ly significant differences between boys and girls in the overt behavior domain and ability domain of CITSEA (t>2.136, P<0.05), and was not in the covert behavior domain and imbalance domain (t<1.172, P>0.05). Conclusion There are gender differences in emotional socialization development of children.

Objective To investigate the expression of angiotensinⅡ (Ang Ⅱ ) and its type 1 receptor (AT1) in circulation, placenta and kidney of the rots preeclampsia. Methods Preedampsia rat model was developed by inhibitor of nitric oxide synthase (L-NAME). The systolic blood pressure (SBP), 24 h urine protein, hepatic and renal function were compared among the precelampsia group, the normal pregnant group and nonpregnant control group. The kidney tissue was observed by light microscopy. ELISA and radioimmunoassay were used to detect Ang Ⅱ in rat plasma and kidney homogenate respectively. Placental AT1 was measured by Westem blot. The level of kidney AT1 was evaluated by immunohistochemistry and Western blot. Results In preeclampsia rats, SBP and 24 h urine protein were significantly higher compared with control groups. Compared to normal pregnant group, plasma Ang Ⅱ of preeclampsia rats was much higher [(0.706±0.086) ng/L vs (0.540±0.085) ng/L, P＜0.05]; placental AT1 was increased by 46%(P＜ 0.05); kidney Ang Ⅱ was decreased signigicantly [(65.543±40.634) ng/g vs (165.543±33.078) ng/g, P＜0.05]. The expression of ATI in kidney of preeclampsia rats was reduced evidently,which was only 33% of normal pregnancy group and 59% of nonpregnant control greup,respeetively (P＜0.05). Conclusions In preeclampsia rat model, the circulating Ang Ⅱ is increased, the placental RAS isactivated, while the kidney BAS is suppressed. The underlying mechanism of proteinuria and kidney damage associated with this phenomenon in preeclampsia needs further research.