1.Effect of Foreign Plasmid DNA on Material and Energy Metabolism of Spleen in Mice
Jian-Wen LIU ; Yong-Hui SHI ; Guo-Wei LE ;
China Biotechnology 2006;0(02):-
To study the influence of foreign plasmid DNA on spleen metabolism in immune-stimulated mice via the gastrointestinal tract. Mice were oral administered by pipette with 200?g of plasmid pcDNA3 and spleen were isolated at 4h and 18h after oral administration. Total RNA were extracted from spleen and spleen gene expression profile of Balb/c mice was analyzed by using Affymetrix oligonucleotide genechip after oral plasmid pcDNA3 administration. Functional cluster analysis was conducted by Genmapp and MAPPFinder software. By functional cluster analyzing the genes which were up-regulated more than twofolds, Genmapp results showed that plenty of metabolic pathway were induced in spleen after oral administration of plasmid pcDNA3. These metabolic process included purine metabolism, pyrimidine metabolism, protein synthesis, cholesterol synthesis, fatty acid synthesis, Glycolysis, TCA cycle and mitochondria oxidative phosphorylation pathway. The similar results also took place at 18h after oral administration. The result indicated that foreign plasmid DNA can modulate metabolism process in spleen of mice via the gastrointestinal tract, and may help understand the mechanism of action of foreign plasmid DNA uptaked via the gastrointestinal tract.
2.Clinical Features and Treatment of Eosinophilic Lymphoid Granuloma in Children
ling, JIN ; yong-hong, ZHANG ; hui-wen, SHI
Journal of Applied Clinical Pediatrics 2006;0(21):-
Objective To explore the clinical features and treatment of eosinophilic lymphoid granuloma(ELG) in children,in order to increase understanding of the disease.Methods Clinical features,laboratory examination,pathological examination,treatment and the-(rapeutic) effect of 5 patients with ELG were retrospectively studied,and the related literature were reviewed.Results Five cases were all boys.They all presented many subcutaneous swellings and lymphadenopathy.A case among them was associated with nephrotic syndrome,and 2 cases had the complication of eczema repeatedly,and 1 case ever had 2 times episodes of asthmatiform bronchitis.All of them had no hepatomegaly and splenomegaly,physically was well developed.Only chemotherapy was supplied to all the patients.During the course of therapy,4 cases among them recurred.After that,they were successfully treated with irradiation of lesion or cyclosporine(CsA).Conclusions ELG in children is as same as adult in clinicopathological features.It tends recur only with chemotherapy.We can get better curative effect with the synthetic treatment of chemotherapy,radiotherapy,operation or oral CsA and so on.
3.Reconstruction of superior peroneal retinaculum by using a portion of Achilles’ s tendon and suture anchors in treatment of chronic peroneal tendon dislocation
Rongmao SHI ; Hui TANG ; Yong SHA ; Yongqing XU
Journal of Regional Anatomy and Operative Surgery 2016;25(3):178-180
Objective To evaluate the clinical outcome of reconstruction of the superior peroneal retinaculum (SPR) by using a portion of Achilles’s tendon and suture anchors in the treatment of chronic peroneal tendon dislocation .Methods Seven patients in our hospital from January 2010 to January 2015 who underwent surgical reconstruction of SPR by using a portion of Achilles ’ s tendon and suture anchors were evaluated .The ankle ’ s range of motion ( ROM) and The American Orthopaedic Foot and Ankle Society ( AOFAS) ankle-hindfoot score were used to evaluate the clinical outcome .Results All 7 cases were followed up for 6 to 24 months,the average time was 14 months,with-out recurrence.The ankle’s ROM improved from (23.6 ±1.9) °preoperative to (30.0 ±1.5) °at the final follow-up,and the AOFAS An-kle Hindfoot Scale improved from (60.6 ±3.6) point preoperative to (93.6 ±3.9) point at the final follow-up.Conclusion Surgical re-construction of SPR by using a portion of Achilles ’ s tendon and suture anchors may be an effective alternative surgical method for chronic peroneal tendon dislocation .
4.31 cases of pneumoconiosis complicated with acute myocardial infarction in coal miners.
Yong WON ; Hui CHIN ; Yu-tzai CHEN ; Shi-hi LIU ; Shi-she WANG ; Wen-sho HSU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(3):171-171
Aged
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Coal Mining
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Female
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Humans
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Male
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Middle Aged
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Myocardial Infarction
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diagnosis
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etiology
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Pneumoconiosis
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complications
5.Role of Wnt/β-catenin signaling pathway in impaired wound healing of diabetes mellitus
Xiaolei SUN ; Yong LIU ; Meihong SHI ; Wu ZHONG ; Hong ZENG ; Hui YANG ; Sen SHI ; Xiangyu ZHOU ; Lixia NIE ; Yanzheng HE
Chinese Journal of Endocrinology and Metabolism 2011;27(1):57-61
Objective To investigate the role of Wnt/β-catenin signaling pathway in impaired wound healing of diabetes mellitus.Methods The back skin defect was produced in rats with type1diabetes.All of these rats were divided into normal group, diabetes group, lithium chloride group, and epidermal growth factor (EGF) group.The back wound healing and β-catenin expression were observed.Results There were no signs of infection in the wound of rats after injury.Compared with diabetic group, the wound healing time was shorter,wound healing rate was higher, wound cavity volume was smaller, granulation tissue was more mature, and β-catenin positive cell rate was higher in normal group, lithium chloride group, and EGF group(P<0.05 or P<0.01).Conclusions Wnt/beta-catenin signaling pathway is involved in the process of wound healing in diabetic rats.
6.The Influence of Peptidoglycan of Lactobacillus on Immune Function of Mouse
Guang-Wei SU ; Jin SUN ; Yong-Hui SHI ; Guo-Wei LE ;
China Biotechnology 2006;0(08):-
Mice was stimulated by peptidoglycan from Lactobacillus sp and detect cytokines production。It was found that PG induced the production of inflammatory cytokines (IL-1,TNF-? in peritoneal macrophages,IFN-? in spleen cell)and did not induce the IL-2 production in spleen cell. Affymetrix MOE430A genechip was used to analyze changed gene expression of immune cells. It was found that expression of cytokines and related genes were changed under peptidoglycan administration. This might induced by activation of TLR-NF-?B signal pathway.
7.Determination of L-carnitine in human seminal plasma by HPLC method and its clinical significance
Ke LI ; Wei LI ; Yong-Hui SHI ; Yu-Feng HUANG ; Xue-Jun SHANG ;
Chinese Journal of Laboratory Medicine 2001;0(02):-
Objective To develop and validate a simple and reliable HPLC method for the analysis of L-carnitine in human seminal plasma and to investigate its clinical significance as a potentially useful index of infertile.Methods After proteins in seminal plasma are precipitated with acetonitrile,L-carnitine in seminal plasma was derivatized to form its ester.HPLC separation of the sample solution was performed on a Lichrospher SiO_2 column and detected by ultraviolet absorbance at 260 nm.A mobile phase composed of acetonitrile-citrie acid buffer(containing 12 mmol/L triethanolamine,pH 5.0)was found to be the most suitable for this separation at a flow rate of 1.2 ml/min and enabled the baseline separation of the L-carnitine from interferences with isocratic elution.The L-carnitine levels in seminal plasma were studied in both 87 patients with infertility and 30 control subjects.Results Under the chromatographic conditions described, the L-carnitine derivative had a retention time of approximately 13 min.Good separation and detectability of L-carnitine in human seminal plasma sample were obtained.The method proved to be linear in the range of L-carnitine from 0 ?mol/L to 1000 ?mol/L.The relative standard deviations of within-and between-assay for L-carnitine analysis were 1.23% and 1.36%,respectively.The recoveries were 91.6% -96.5% for the human seminal plasma samples.L-carnitine concentrations in the populations were(392.7?107.2)?mol/L in the fertile group(n=30),(270.0?83.9)?mol/L in asthenozoospermia group(n=29),(187.9? 43.9)?mol/L in oligozoospermia group(n=19)and(175.7?67.1)?mol/L in oligoasthenozoospermia group(n=39).The large difference(P0.05).Conclusion The determination of L-carnitine level in seminal plasma may prove useful as a potentially biochemical marker of fertility and this is a useful guidance for the clinic therapy and the mechanismic study on the male reproduction.
8.Study of clinicopathologic features and p53 gene alterations in renal epithelioid angiomyolipoma.
Yan ZHU ; Juan-hong SHI ; Jing ZHAO ; Jing-jing XU ; Lin-hui WANG ; Yong-wei YU
Chinese Journal of Pathology 2012;41(8):558-559
Adenoma, Acidophil
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metabolism
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Adult
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Angiomyolipoma
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genetics
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metabolism
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pathology
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Carcinoma, Renal Cell
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metabolism
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Codon
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Diagnosis, Differential
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Exons
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Female
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Follow-Up Studies
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Gene Deletion
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Genes, p53
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Humans
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Kidney Neoplasms
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genetics
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metabolism
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pathology
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Male
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Melanoma-Specific Antigens
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metabolism
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Middle Aged
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Retrospective Studies
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Tumor Suppressor Protein p53
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genetics
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metabolism
9.Clinical study on Qinghouyan lozenge in treatment of acute pharyngitis.
Jiao-iiao YU ; Zhen-yu XUAN ; Yan RUAN ; Hui-yong ZHANG ; Ke-hua SHI ; Yu GUO
China Journal of Chinese Materia Medica 2015;40(2):351-355
To evaluate the clinical efficacy and safety of Qinghouyan lozenge in the treatment of acute pharyngitis due to Lung-heat and Yin-deficiency, and compare with Qinghouyan oral Liquid. Totally 144 subjects were enrolled and randomly divided into two groups (72 in the test group and 72 in the control group). The participants in the test group were given Qinghouyan lozenge for 5 days, and those in the control group were given Qinghouyan oral Liquid for 5 days. The effectiveness evaluation indexes were pharyngalgia/odynophagia disappearance rate, overall efficacy of TCM syndromes, TCM syndrome scores, and single syndrome and sign disappearance rate. During the test, the safety was evaluated by vital sign, lab examination indexes and adverse events. The results for the full analysis set showed that the couth disappearance rate, the incidence rate of TCM syndromes, and the throat/uvula congestion disappearance rate of the test group were higher than that of the control group (P < 0.05), with significant differences in the changes in syndrome scores between the two groups (P < 0.05). Altogether 3 adverse events were observed in the test group while 6 adverse events in the control group, without significant differences in the adverse event rate between the two groups (P < 0.05), serious abnormal laboratory examinations and vital signs. In conclusion, Qinghouyan lozenge has better efficacy in treatment of acute pharyngitis due to Lung-heat and Yin-deficiency than Qinghouyan oral liquid, with good safety.
Acute Disease
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Double-Blind Method
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Humans
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Medicine, Chinese Traditional
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Pharyngitis
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drug therapy
10.Treatment of retinal detachment after laser in situ kera-tomileusis
Ling-Yan, CHEN ; Lin, LÜ ; Yong-Hao, LI ; Shi-Yi, LI ; Hui, ZHONG ; Wang, FANG
International Eye Science 2011;11(6):947-950
AIM: To study the characteristics of retinal detachment surgery after laser in situ keratomileusis (LASIK).METHODS: Eleven eyes of ten patients that experienced rhegmatogenous retinal detachment after LASIK procedure participated in the study. The characteristics of retinal detachment, management and complications after surgery were analyzed . RESULTS: Retinal detachment was characterized by the large percentage of multiple peripheral holes (73%) and giant tears (27%). All eyes underwent sclera buckling, and three of them combined with pars plana vitrectomy (PPV) and silicone oil tamponade. Silicone oil was removed after 1 month. Retina was reattached successfully at the first retinal detachment surgery in all eyes except one that succeeded at the fourth time. One eye of LASIK flap dehiscence and one eye of corneal subepithelial opacity occurred after surgery.CONCLUSION: Patients after LASIK should be carefully examined under pupillary dilation during follow-up. Sclera buckling is necessary to most retinal detachment after LASIK, and corneal protection is important in the treatment.