PURPOSE: To evaluate the spatial distribution of various proton metabolites in the human brain with use of water-suppressed in vivo 1H MR spectroscopic imaging (MRSI) technique MATERIALS AND METHODS: All of water-suppressed in vivo 1H MRSI were performed on 1.5 T whole-body MRI/MRS system using Stimulated Echo Acquisition Method (STEAM) Chemical shift Imaging (CSI) pulse sequence. T1 -weighted MR images were used for CSl Field Of View (FOV; 24 cm). Voxel size of 1.S cm3 was designated from the periphery of the brain which was divided by 1024 x 16 x 16data points. RESULTS: Metabolite images of N-acetylaspartate (NAA), creatine/phosphocreatine (Cr) + choline/phosphocholine (Cho), and complex of gamma-aminobutyric acid (GABA) -I- glutamate (Glu) were obtained on the human brain. CONCLUSION: Our preliminary study suggests that in vivo 1H MRSl could provide the metabolite imaging to compensate for hypermetabolism on Positron Emission Tomography (PET) scans on the basis of the metabolic informations on brain tissues. The unique ability of in vivo 1H MRSI to offer noninvasive informations about tissue biochemistry in disease states will stimulate on clinical research and disease diagnosis.
Biochemistry ; Brain* ; Diagnosis ; gamma-Aminobutyric Acid ; Glutamic Acid ; Humans* ; Magnetic Resonance Imaging ; Positron-Emission Tomography ; Protons

Association of the hepatoid and choriocarcinomatous components in adenocarcinoma of the stomach is extremely unusual and raises a possibility of new approach understand the histogenesis of gastric hepatoid adenocarcinoma. This paper describes a Borrmann type III adenocarcinoma of the stomach with both choriocarcinomatous and hepatoid components in composite tumor pattern in a 50-year-old man. Tubular arrangement of differentiated embryonalcarcinoma was encountered in choricarcinomatous and hepatoid areas, which showed strong immunoreactivity to beta-HCG and AFP, respectively. The findings suggest that gastric adenocarcinoma may have a potential of differentiation toward embryonal carcinoma. from which both choriocarcinoma and hepatoid variant of gastric adenocarcinoma may develop by retrodifferentiation.
Adenocarcinoma

A computer program using automatic coding of the diagnosis has been used for report printing as well as data storage and retrieval system at the Department of Pathology, Seoul National University Hospital. Previous surgical pathology files were also computerized by the automatic coding system using personal computer, and 100,000 cases of surgical pathology data during 7 years were computerized at present. The cpmputerized surgical files were counted and listed according to topograph and morphologic diagnosis. It is available to print out the list of a specific diagnosis or to copy the records to a floppy diskette. Collection of cases in surgical pathology files using cpmputerized automatic coding system becomes much convenient and accurate than using stored file cards or log books. In addition, previous biopsy records of the patient are automatically searched during the routine work so that understanding of a patient as a whole is possible through the informations about previous pathologic diagnosis. We confirm that automatic coding methods is the most practical and economic method for computerization of the surgical pathology records.
Biopsy

A computer program using automatic coding of the diagnosis has been used for report printing as well as data storage and retrieval system at the Department of Pathology, Seoul National University Hospital. Previous surgical pathology files were also computerized by the automatic coding system using personal computer, and 100,000 cases of surgical pathology data during 7 years were computerized at present. The cpmputerized surgical files were counted and listed according to topograph and morphologic diagnosis. It is available to print out the list of a specific diagnosis or to copy the records to a floppy diskette. Collection of cases in surgical pathology files using cpmputerized automatic coding system becomes much convenient and accurate than using stored file cards or log books. In addition, previous biopsy records of the patient are automatically searched during the routine work so that understanding of a patient as a whole is possible through the informations about previous pathologic diagnosis. We confirm that automatic coding methods is the most practical and economic method for computerization of the surgical pathology records.
Biopsy

No abstract available.
Arthritis* ; Osteoarthritis* ; Transforming Growth Factor beta* ; Transforming Growth Factors*

Eight cases of combine hepatocellular-cholangiocarcinoma(HCC-CC) of the liver were analysed along with their growth patterns and histologic subtypes to draw a possible implication in understanding of their histogenesis. The relative incidence of combined HCC-CC among the surgically resected primary carcinomas of the liver(485 cases) was 1.6%. The combination patterns varied and were classified as follows; the multinodular tumor, each consisting of HCC or CC element(type 1) was found in 1 case, the single tumor mass with two distinct compartments of HCC and CC(type 2) in 3, and the single tumor with random mixture of two elements(type 3) comprised the remaining 4 cases. Of the 7 cases of single tumor mass(type 2 and 3) HCC and CC comprised the major component in 5 and 2 cases, respectively. The histologic features of both HCC and CC elements were not different from those of isolated HCC and CC, except for two of CC element in type 3 which disclosed the intermediated differentiation or socalled cholangiolocellular carcinoma in part. We conclude that there is no significant difference in the relative incidence of combined HCC-CC among primary carcinomas of the liver and their subtypes compared to that in other countries, regardless of high incidence of both HCC and CC in Korea. Also, we discussed a possible histogenisis along a hypothesis that some of the combined HCC-CC be the consequence of interposition of different cell type from a new subclone into the growth of an initial single cell type of primary carcinoma of the liver.
Incidence ; Carcinoma, Hepatocellular

No abstract available.
Apolipoproteins* ; Blood Pressure* ; Child* ; Cholesterol* ; Humans ; Hyperlipidemias ; Hypertension ; Obesity* ; Potassium* ; Sodium* ; Statistics as Topic*

No abstract available.
Arteriovenous Malformations*

In order to assess the extracellular matrix(ECM) secretion of corneal epithelial cells inoculated with herpes simplex virus-1 in vitro the indirect immunofluorescent technique was used. In this study, ECM such as fibronectin, type IV collagen and laminin were rich in cultured rabbit corneal epithelial cells under the normal condition, while those became poor after inoculation with herpes simplex virus-1. This result suggested that herpes simplex virus-1 inhibit ECM secretion of the corneal epithelial cells. These findings mean that delayed epithelial wound healing after herpes simplex virus-1 infection is due to suppressed secretion of ECM which is the principal component of corneal epithelial wound healing.
Collagen Type IV ; Epithelial Cells* ; Extracellular Matrix* ; Fibronectins ; Herpes Simplex ; Herpesvirus 1, Human* ; Laminin ; Simplexvirus ; Wound Healing

This study was performed to observe the retinal changes in rabbits by intravitreal inoculation of HSV-1. The solution of HSV-1 virus(Kos strain) was inoculated into the vitreous cavity in 5 eyes. All the eyes were checked with a slit lamp and an indirect ophthalmoscope. Two eyes that showed the retinal lesion were enucleated for the histopathologic examination. The focal infiltration of inflammatory cells was marked around the retinal vessels in one eye and there was the diffuse infiltration of plasma cells and lymphocytes in the choroid and the retina in the other eye. The pattern of proliferation and migration of retinal pigment epithelial cells was observed at the outer retina. The virus-infected nuclei were evident in the photoreceptor cells and ganglion cells. The viral particles and intranuclear inclusion were prominent in the deformed nuclei and free-floating viral particle was shown at the extracellular space of the necrotic retina. These results suggest that the intravitreal inoculation of HSV-1 might induce retinal necrosis. The inflammatory reactions was initiated at the vitreoretinal interface and perivascular area. Virus might be propagated through axons or infected cell from free-flating virus.
Axons ; Choroid ; Epithelial Cells ; Extracellular Space ; Ganglion Cysts ; Herpes Simplex* ; Herpesvirus 1, Human ; Intranuclear Inclusion Bodies ; Lymphocytes ; Necrosis* ; Ophthalmoscopes ; Photoreceptor Cells ; Plasma Cells ; Rabbits ; Retina ; Retinal Vessels ; Retinaldehyde* ; Simplexvirus ; Virion